165,075 results

1. pTcI42-Wasabi

   Plasmid

   #86118

   Purpose

   mWasabi reporter gene gated by temperature-sensitive Lambda repressor TcI42

   Depositor

   Mikhail Shapiro

   Article

   Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

   Inserts

   TcI42
   mWasabi
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   A67T, K6N, S33T, Y61H, L119P, F122C

   Promoter

   pR/pL and pR/pL, LacI

   Available Since

   Feb. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKm-HABE

   Plasmid

   #170332

   Purpose

   a modularized single-plasmid adenosine base editing (A-to-G) tool for Sinorhizobium meliloti

   Depositor

   Deqiang Duanmu

   Article

   Wang et al Front Microbiol. 2021 Jun 18;12:686008.

   Insert

   Rhizobium codon-optimized ABEs
   

   Use

   CRISPR

   Mutation

   Cas9n-D10A

   Available Since

   Aug. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. R777-E035 Hs.DUSP2

   Plasmid

   #70319

   Purpose

   Gateway ORF clone of human DUSP2 [NM_004418.3] with stop codon (for native or N-terminal fusions)

   Depositor

   Dominic Esposito

   Insert

   DUSP2 (DUSP2 Human)
   

   Use

   Gateway entry clone

   Available Since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. EXT1- D164A-pLJC2-3XFLAG

   Plasmid

   #215828

   Purpose

   Ectopic expression of catalytically inactive mutant (EXT1-D164A-FLAG) in mammalian cells

   Depositor

   Yoav Shaul

   Article

   Solaimuthu et al Proc Natl Acad Sci U S A. 2024 Jan 16;12

   Insert

   Exostosin glycosyltransferase 1 Mutant (EXT1 Human)
   

   Use

   Lentiviral

   Tags

   3X Flag

   Expression

   Mammalian

   Available Since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLZ12Km2-P23R:TA:fflucRT

   Plasmid

   #88901

   Purpose

   Toxin-antitoxin stabilized bioluminescent reporter plasmid for Streptococcus expression

   Depositor

   Thomas Proft

   Article

   Loh et al Appl Microbiol Biotechnol. 2013 Nov;97(2

   Insert

   toxin-antitoxin + Red-shifted firefly luciferase
   

   Expression

   Bacterial

   Available Since

   April 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. mH2A1.1-CT-MYC

   Plasmid

   #45166

   Depositor

   Brian Chadwick, Hunt Willard

   Article

   Chadwick et al Hum Mol Genet. 2001 May 1;10(10):1101-13

   Insert

   H2Afy (MACROH2A1 Human)
   

   Tags

   HIS and MYC

   Expression

   Mammalian

   Available Since

   July 2, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV_CAG_FLEX_PKIαmut-P2A-mRuby3_WPRE

   Plasmid

   #196678

   Purpose

   AAV plasmid for mammalian Cre-dependent expression of PKIαmut-P2A-mRuby3 under a CAG promotor w/ WPRE enhancer. PKIαmut bears the R20G/R21G mutation that renders it non-functional.

   Depositor

   Haining Zhong

   Article

   Ma et al Nature. 2022 Nov;611(7937):762-768. doi:

   Insert

   PKIαmut-P2A-mRuby3
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Available Since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-TORCAR-NLS

   Plasmid

   #64931

   Purpose

   Nuclear-targeted FRET biosensor for monitoring mTORC1 kinase activity.

   Depositor

   Jin Zhang

   Article

   Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

   Insert

   TORCAR-NLS (EIF4EBP1 Human, Synthetic)
   

   Tags

   Cerulean, Nuclear localization signal (NLS), and …

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTcI-Wasabi

   Plasmid

   #86101

   Purpose

   mWasabi reporter gene gated by temperature-sensitive Lambda repressor TcI

   Depositor

   Mikhail Shapiro

   Article

   Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

   Inserts

   TcI
   mWasabi
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   A67T

   Promoter

   pR/pL and pR/pL, LacI

   Available Since

   Feb. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-CaMKIIa-eArchT3.0-P2A-EGFP-WPRE-hGHpA

    Plasmid

    #51110

    Purpose

    Forebrain principal neuron expression of eArchT3,0 with physically uncoupled EGFP fluorophore

    Depositor

    Jonathan Ting

    Insert

    eArchT3.0
    

    Use

    AAV

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Promoter

    CaMKIIa

    Available Since

    April 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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