We narrowed to 9,193 results for: Pol;

1. pET21a - SodB

   Plasmid

   #89613

   Purpose

   Expresses recombinant 6HIS tagged T. cruzi superoxide dismutase B protein in E. coli upon IPTG induction

   Depositor

   Judy Lieberman

   Article

   Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

   Insert

   Superoxide dismutase B
   

   Tags

   6HIS and T7 tag (gene 10 leader)

   Expression

   Bacterial

   Available Since

   April 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSicoR-mCh-Chd1i1

   Plasmid

   #21904

   Depositor

   Miguel Ramalho-Santos

   Article

   Gaspar-Maia et al Nature. 2009 Aug 13;460(7257):863-8. doi

   Insert

   Chd1-i1
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   Sept. 30, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET:ACAT:GFP

   Plasmid

   #60740

   Purpose

   A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'ACAT' between -11 and -8.

   Depositor

   Matthew Bennett

   Article

   Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

   Insert

   GFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Nov. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. CS2-GFP-Ben

   Plasmid

   #47966

   Purpose

   Expression of fly Ben

   Depositor

   Laura Lee

   Article

   Merkle et al Development. 2009 Feb;136(3):449-59. doi

   Insert

   Bendless (ben Fly)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Dec. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

5. CAG-EGFP-Otx2shRNA2

   Plasmid

   #73982

   Purpose

   Otx2 shRNA2 (based on mir155) was inserted into the 3'UTR of EGFP.

   Depositor

   Connie Cepko

   Article

   Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

   Insert

   EGFP-Otx2-shRNA2
   

   Use

   RNAi

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. CAG-mCherry-CrxshRNA

   Plasmid

   #73987

   Purpose

   Crx shRNA (mir30 backbone) was inserted into the 3'UTR of mCherry.

   Depositor

   Connie Cepko

   Article

   Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

   Insert

   shRNA that targets the mouse Crx gene
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. AL13Rb-HsfA2

   Plasmid

   #161012

   Purpose

   PP7-tagged reporter driven by the Arabidopsis HsfA2 promoter

   Depositor

   Krishna Niyogi

   Article

   Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

   Insert

   PP7 and H2B-mScarlet
   

   Expression

   Plant

   Available Since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLMB684

   Plasmid

   #83779

   Purpose

   Xylose biosensor plasmid

   Depositor

   Philip Poole

   Article

   Pini et al Plant Physiol. 2017 Jul;174(3):1289-1306

   Insert

   upstream region of RL2720 (rbsC)
   

   Available Since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. CAG-EGFP-Otx2shRNA1

   Plasmid

   #73981

   Purpose

   Otx2 shRNA1 (mir155 backbone) was inserted into the 3'UTR of EGFP.

   Depositor

   Connie Cepko

   Article

   Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

   Insert

   shRNA that targets the mouse Otx2 gene
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. CRISPRmTmG2

    Plasmid

    #69992

    Purpose

    The CRISPR construct targets near the LoxP sites in Rosa-pCA-loxP-mTdtomato-loxP-mEGFP mice.

    Depositor

    Connie Cepko

    Article

    Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

    Insert

    gRNA that targets near LoxP sites
    

    Available Since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCellFree_G03 PPARG

    Plasmid

    #67099

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    PPARG (PPARG Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pKM149

    Plasmid

    #134178

    Purpose

    Bacterial expression of his-CENP-C 510-end

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Cell. 2014 Jul 17;158(2):397-411. doi: 1

    Insert

    his-CENP-C 510-end (CENPC Human)
    

    Expression

    Bacterial

    Mutation

    aa 510-end

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLMB687

    Plasmid

    #83781

    Purpose

    Sucrose biosensor plasmid

    Depositor

    Philip Poole

    Article

    Pini et al Plant Physiol. 2017 Jul;174(3):1289-1306

    Insert

    Region upstream pRL120556 containing all of pRL120555
    

    Available Since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSicoR-mCh-Chd1i4

    Plasmid

    #21908

    Depositor

    Miguel Ramalho-Santos

    Article

    Gaspar-Maia et al Nature. 2009 Aug 13;460(7257):863-8. doi

    Insert

    Chd1-i4
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Sept. 30, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBM23-pyrF-NT

    Plasmid

    #174384

    Purpose

    pBM23 contained pyrF and mini-CRISPR.

    Depositor

    Jing Han

    Article

    Lin et al Commun Biol. 2021 Aug 25;4(1):1007. doi:

    Insert

    pyrF-NT
    

    Use

    Template for cloning fragments for chromosomal in…

    Expression

    Bacterial

    Available Since

    Nov. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHFX-B60-EPSUD

    Plasmid

    #174390

    Purpose

    Plasmids for gene knockout were constructed based on suicide plasmid pHFX-B60

    Depositor

    Jing Han

    Article

    Lin et al Commun Biol. 2021 Aug 25;4(1):1007. doi:

    Insert

    EPSUD
    

    Use

    Archaeal gene knockout vector for haloferax medit…

    Available Since

    Nov. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBM23-pyrF-CS

    Plasmid

    #174396

    Purpose

    pBM23 contained pyrF and mini-CRISPR.

    Depositor

    Jing Han

    Article

    Lin et al Commun Biol. 2021 Aug 25;4(1):1007. doi:

    Insert

    pyrF-CS
    

    Use

    Template for cloning fragments for chromosomal in…

    Expression

    Bacterial

    Available Since

    Nov. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. CTAG_linker-eGFP

    Plasmid

    #136098

    Purpose

    FP for C-term fusion with CDS12 without linker

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    CTAG_AlaSerGly10xAlaGlySer_eGFP
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. CTAG_linker-mTurquoise2

    Plasmid

    #136100

    Purpose

    FP for C-term fusion with CDS12 without linker

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    CTAG_AlaSerGly10xAlaGlySer_mTurquoise2
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Available Since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. CDS_GUS

    Plasmid

    #136084

    Purpose

    For histochemical _-glucuronidase activity assays. This GUS CDS does not have an intron.

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    CDS_GUS
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Available Since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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