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pLenti6.2_3xFLAG_V5_ mmIfih1_V5

Plasmid

#160110

Purpose

Expresses murine Ifih1 in mammalian cells

Depositor

Rizwan Haq

Publication

Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

Insert

Ifih1 (Ifih1 Mouse)

Use

Lentiviral

Tags

3xFLAG, V5 and V5

Expression

Mammalian

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA6-N-Flag-SREBP2

Plasmid

#134284

Purpose

Expresses Flag-tagged SREBP2 in mammalian cells

Depositor

Bruce Beutler

Publication

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

SREBP2 (Srebf2 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

mouse SREBP2 precursor

Promoter

CMV

Available since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pMTL3

Plasmid

#127966

Purpose

constitutive expression of KRAB-dCas9-BFP-KRAB from the AAVS1 locus

Depositor

Martin Kampmann

Publication

Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

AAVS1-CAG-KRAB-dCas9-BFP-KRAB

Use

TALEN

Expression

Mammalian

Promoter

CAG

Available since

Nov. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pMTL5

Plasmid

#127967

Purpose

DHFR-degron controlled expression of KRAB-dCas9-BFP-KRAB from the AAVS1 locus

Depositor

Martin Kampmann

Publication

Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

AAVS1-CAG-DHFR-KRAB-dCas9-BFP-KRAB

Use

TALEN

Expression

Mammalian

Promoter

CAG

Available since

Nov. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pFRT_TO_FlagHA_USP10

Plasmid

#127102

Depositor

Thomas Tuschl

Publication

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

USP10 (USP10 Human)

Expression

Mammalian

Available since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHAGE-XPO1

Plasmid

#116804

Purpose

Lentiviral expression of XPO1

Depositor

Gordon Mills, Kenneth Scott

Publication

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

XPO1 (XPO1 Human)

Use

Lentiviral

Mutation

WT

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHAGE-PDGFRB

Plasmid

#116770

Purpose

Lentiviral expression of PDGFRB

Depositor

Gordon Mills, Kenneth Scott

Publication

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PDGFRB (PDGFRB Human)

Use

Lentiviral

Mutation

WT

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pHAGE-CDK6

Plasmid

#116725

Purpose

Lentiviral expression of CDK6

Depositor

Gordon Mills, Kenneth Scott

Publication

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

CDK6 (CDK6 Human)

Use

Lentiviral

Mutation

WT

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pET-mKelly2

Plasmid

#119783

Purpose

Expresses mKelly2 tagged N-terminally with an MHHHHHHGSG tag

Depositor

Stephen Mayo

Publication

Wannier et al Proc Natl Acad Sci U S A. 2018 Nov 27;11

Insert

mKelly2

Tags

6xHis tag

Expression

Bacterial

Promoter

T7

Available since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-SpdCas9-VP64

Plasmid

#115794

Purpose

Expresses SpdCas9-Vp64 fusion protein when packaged into AAV

Depositor

Nadav Ahituv

Publication

Matharu et al Science. 2018 Dec 13. pii: science.aau06

Insert

VP64

Use

AAV

Tags

3XFLAG and dCas9-VP64

Expression

Mammalian

Promoter

CMV

Available since

Jan. 19, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCDF-MCM4/6

Plasmid

#116951

Purpose

Expresses human MCM4 and human MCM6

Depositor

James Chong

Publication

Hesketh et al J Biol Chem. 2015 Mar 20;290(12):7973-9.

Inserts

Human MCM6 (MCM6 Human)
Human MCM4 (MCM4 Human)

Tags

His6

Expression

Bacterial

Available since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

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p2K7bsdUBI-mCherry-STIM1

Plasmid

#114178

Purpose

Lentiviral vector for expression of mCherry-STIM1

Depositor

Nicolas Demaurex

Publication

Nunes-Hasler et al Nat Commun. 2017 Nov 24;8(1):1852. doi:

Insert

STIM1 (STIM1 Human)

Use

Lentiviral

Tags

mCherry (inserted after signal peptide)

Expression

Mammalian

Promoter

Ubiquitin

Available since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAJM.847

Plasmid

#108524

Purpose

PhlFAM + PPhlF-YFP Reporter. DAPG sensor for use in non-Marionette strain.

Depositor

Christopher Voigt

Publication

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

PhlFAM + PPhlF-YFP

Expression

Bacterial

Mutation

PhlF: S50G S73I R95H A121V M137I N144D T155I R200C

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMBP-AaCas12b

Plasmid

#113433

Purpose

Expresses Expresses 10xHis-MBP fused to AaCas12b

Depositor

Jennifer Doudna

Publication

Chen et al Science. 2018 Apr 27;360(6387):436-439.

Insert

AaCas12b

Tags

MBP

Expression

Bacterial

Promoter

T7

Available since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAJM.771

Plasmid

#108534

Purpose

NahRAM +PSalTTC-YFP Reporter. Sal sensor for use in non-Marionette strain.

Depositor

Christopher Voigt

Publication

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

NahRAM + PSalTTC-YFP

Expression

Bacterial

Mutation

NahR: I25V P72H V118A R248C

Available since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLenti-xCas9RA-P2A-Puro

Plasmid

#110871

Purpose

Lentiviral vector for constitutive expression of xCas9(3.7)RA in mammalian cells (codon optimized)

Depositor

Lukas Dow

Publication

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

xCas9(3.7)RA

Use

Lentiviral

Tags

FLAG

Mutation

A262T, R324L, S409I, E480K, E543D, M694I, E1219V

Promoter

EF1s

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1D V5-His-TOPO-KGA.wt

Plasmid

#110332

Purpose

Mammalian expression of glutaminase isoform KGA (wild type) with V5 and 6xHis tags

Depositor

Sandra Martha Gomes Dias

Publication

Cassago et al Proc Natl Acad Sci U S A. 2012 Jan 24;10

Insert

KGA glutaminase (GLS Human)

Tags

6xHis and V5

Expression

Mammalian

Promoter

CMV

Available since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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plenti-UbcP-HA-CRBN-pGK-HYG

Plasmid

#107378

Purpose

Lentiviral expression of HA-CRBN

Depositor

William Kaelin

Publication

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

cereblon (CRBN Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

UbcP

Available since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBBR1k-con-GFPuv

Plasmid

#106386

Purpose

Constitutive trc promoter expressing gfpuv on BBR1 origin

Depositor

Brian Pfleger

Publication

Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

Insert

GFPuv

Expression

Bacterial

Promoter

trc

Available since

March 22, 2018

Availability

Academic Institutions and Nonprofits only

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YET1000: CAG-human dLbCpf1(D832A)-NLS-3xHA-3xFLAG-DmrA(X4)

Plasmid

#104571

Purpose

Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to four DmrA domains

Depositor

Keith Joung

Publication

Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

human codon optimized ‘dead’ Cpf1 fused to four DmrA domains

Use

CRISPR

Tags

NLS-3xHA-3xFLAG-4xDmrA

Expression

Mammalian

Mutation

D832A

Promoter

CAG

Available since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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