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pEMF037

Plasmid

#157634

Purpose

Batis maritima MHT

Depositor

Jonathan Silberg

Article

Fulk et al ACS Synth Biol. 2020 Nov 20;9(11):3104-3

Insert

MHT

Expression

Bacterial

Available Since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV4-Tax(M20)

Plasmid

#23285

Depositor

Warner Greene

Article

Smith et al Genes Dev. 1990 Nov . 4(11):1875-85.

Insert

Tax(M20)

Expression

Mammalian

Mutation

T130A L131S

Available Since

May 3, 2010

Availability

Academic Institutions and Nonprofits only
LentiGuide-BC-start

Plasmid

#127168

Purpose

Entry plasmid for two-step guide-barcode cloning

Depositor

Paul Blainey

Article

Feldman et al Cell. 2019 Oct 17;179(3):787-799.e17. do

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

N/A

Available Since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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pET22b-CPDSacI

Plasmid

#38255

Depositor

Matthew Bogyo, Aimee Shen

Article

Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

Type

Empty backbone

Tags

6xHis and Vibrio cholerae MARTX toxin cysteine pr…

Expression

Bacterial

Promoter

T7

Available Since

July 19, 2012

Availability

Academic Institutions and Nonprofits only

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pDONR223_NKX2-5

Plasmid

#221479

Purpose

Gateway cloning of NKX2-5 overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-5 (NKX2-5 Human)

Use

Gateway donor vector

Available Since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pMAH-ER-bapaUGAc

Plasmid

#201674

Purpose

boronic acid- and peptide-assisted UDP-GlcNAc sensor in ER

Depositor

Huiwang Ai

Article

Zhang et al ACS Sens. 2023 Jul 22. doi: 10.1021/acss

Insert

ER-bapaUGAc

Tags

ER leading sequence and ER retention sequence

Expression

Mammalian

Promoter

CMV SP6

Available Since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA4TO-CAMSAP1L1-24xGCN4-IRES-puro

Plasmid

#60912

Purpose

Enocdes Camsap1L1 fused to the SunTag for single molecule imaging

Depositor

Ron Vale

Article

Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

Insert

Camsap1L1

Expression

Mammalian

Available Since

March 13, 2015

Availability

Academic Institutions and Nonprofits only

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pLRD17

Plasmid

#208269

Purpose

Production of syn-stemod-13(17)-ene in E. coli MEV20

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Inserts

tHMGR
osCPS4
osKSL11

Expression

Bacterial

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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CF-iSH

Plasmid

#20159

Depositor

Tobias Meyer

Article

Suh et al Science. 2006 Dec 1. 314(5804):1454-7.

Insert

CFP-FKBP-p85 (Pik3r2 Mouse)

Expression

Mammalian

Available Since

March 24, 2009

Availability

Academic Institutions and Nonprofits only

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pBAD-D4

Plasmid

#37473

Depositor

Amy Palmer, Roger Tsien

Article

Palmer et al Chem Biol. 2006 May;13(5):521-30.

Insert

D4 second generation cameleon (calcium sensor)

Expression

Bacterial

Promoter

Ara

Available Since

Oct. 11, 2012

Availability

Academic Institutions and Nonprofits only
pWS3805

Plasmid

#185746

Purpose

Sub-array spacer 1/4

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Insert

CRISPRai sub-array spacer 1/4

Use

CRISPR

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pWS3806

Plasmid

#185747

Purpose

Sub-array spacer 2/4

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Insert

CRISPRai sub-array spacer 2/4

Use

CRISPR

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pFF530

Plasmid

#61447

Purpose

IPTG-inducible msd_kanR(ON)

Depositor

Timothy Lu

Article

Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

Insert

msd_kanR(ON)

Use

Synthetic Biology

Promoter

pLacO

Available Since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pRec

Plasmid

#104572

Purpose

Expresses Cas1, Cas2 under PLTetO-1 promoter, and TetR and LacI constitutively.

Depositor

Harris Wang

Article

Sheth et al Science. 2017 Dec 15;358(6369):1457-1461

Insert

PTet-cas12, tetR, lacI

Use

Synthetic Biology

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pCFD7

Plasmid

#73916

Purpose

gRNA vector for AsCpf1

Depositor

Simon Bullock

Article

Port et al Nat Methods. 2016 Oct;13(10):852-4. doi:

Insert

tRNA:hAsCpf1-gRNA:tRNA

Use

CRISPR

Expression

Insect

Promoter

dU6:3

Available Since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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P-BT3332

Plasmid

#188110

Purpose

pBolux harboring the promoter region upstream of BT3332

Depositor

Guy Townsend

Article

Modesto et al Nat Commun. 2023 Jan 17;14(1):275. doi:

Insert

The B. thetaiotaomicron BT3332 promoter cloned into pBolux

Expression

Bacterial

Promoter

BT3332

Available Since

Jan. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pBJ077_zACNE20_E1b_2xLyn_mCherry

Plasmid

#209281

Purpose

Expresses membrane-labelled mCherry in the zebrafish heart

Depositor

Adam Cohen

Article

Jia et al Nature. 2023 Oct;622(7981):149-155. doi:

Insert

mCherry2

Use

Zebrafish expression

Promoter

zACNE20_E1b

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pACS125

Plasmid

#204593

Purpose

GST-lamin A C522A (389-646)

Depositor

Tom Misteli

Article

Schibler et al Elife. 2023 May 23;12:e80653. doi: 10.75

Insert

human lamin A C522A (389-646)

Tags

GST - lamin A C522A (389-646)

Expression

Bacterial

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-myc- GFP-Flag

Plasmid

#82471

Purpose

Expresses myc-GFP-Flag

Depositor

Daniel Schoenberg

Article

Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

Insert

Myc-GFP-Flag

Tags

Flag and Myc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pUK

Plasmid

#64185

Purpose

harbours marker genes URA3 and LYS2

Depositor

Markus Ralser

Article

Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

Aug. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pSB1C3-pT3-tetR-sfGFP

Plasmid

#140871

Purpose

T3 promoter driven synthesis of TetR-sfGFP

Depositor

Neal Devaraj

Article

Niederholtmeyer et al Nat Commun. 2018 Nov 28;9(1):5027. doi:

Insert

TetR-sfGFP expressed with T3 promoter

Use

Synthetic Biology

Tags

sfGFP

Expression

Bacterial

Promoter

T3

Available Since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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pLK

Plasmid

#64186

Purpose

harbours marker genes LEU2 and LYS2

Depositor

Markus Ralser

Article

Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

Aug. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pICSL11137

Plasmid

#191783

Purpose

Kanamycin resistance in plants

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

35S::nptII-T-Nos

Expression

Plant

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pFRT-TODestGFP_HuC

Plasmid

#65762

Purpose

expresses GFP-tagged HuC in mammalian cells

Depositor

Thomas Tuschl

Insert

ELAVL3 (ELAVL3 Human)

Tags

FLAG HA and V5

Expression

Mammalian

Promoter

CMV, 2x Tet

Available Since

June 4, 2015

Availability

Academic Institutions and Nonprofits only

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GPI-Gamillus

Plasmid

#197914

Purpose

Monomer expression vector with plasma membrane localization signal

Depositor

Salvatore Chiantia

Article

Petrich et al PLoS One. 2023 Aug 3;18(8):e0285486. doi

Insert

glycosylphosphatidylinositol-Gamillus

Expression

Mammalian

Mutation

none

Available Since

April 19, 2023

Availability

Academic Institutions and Nonprofits only

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pWJ1

Plasmid

#90484

Purpose

For gene disruption in Clostridium acetobutylicum ATCC 824 and Clostridium beijerinckii NCIMB8052

Depositor

Sheng Yang

Article

Zhang et al J Microbiol Methods. 2015 Jan;108:49-60.

Insert

pIM13 Origin

Expression

Bacterial

Available Since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pTY137 psfGFP-bimax2

Plasmid

#202410

Purpose

Expresses sfGFP-bimax2 to inhibit importin-alpha-mediated nuclear import

Depositor

Tim Mitchison

Article

Yoo et al J Cell Biol. 2021 Jul 5;220(7). pii: 212

Insert

bimax2

Tags

sfGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pKS2

Plasmid

#24625

Depositor

Jay Keasling

Article

Steen et al Nature. 2010 Jan 28;463(7280):559-62.

Insert

'tesA

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 27, 2010

Availability

Academic Institutions and Nonprofits only

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pBSV2_2

Plasmid

#118226

Purpose

Empty E. coli - B. burgdorferi shuttle vector; kanamycin resistant

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

Type

Empty backbone

Expression

Bacterial

Available Since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

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EZ-L545

Plasmid

#173024

Purpose

Encodes OptoEXP2 circuit

Depositor

Jose Avalos

Article

Zhao et al ACS Synth Biol. 2021 May 21;10(5):1143-1

Insert

VP16-EL222

Tags

SV40 NLS

Expression

Yeast

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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