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pDEST-pSP172BSSPE-Swa1::RfA-HA

Plasmid

#186405

Purpose

Destination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter HA tag under control of a reg. sequence in the Swa1 restriction site.

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Type

Empty backbone

Use

Gateway destination vector

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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PDEST-pSP172BSSPE-pFOGc::RfA-HA

Plasmid

#186406

Purpose

Destination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter HA tag under control of the pFOG ectodermal regulatory sequence.

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Type

Empty backbone

Use

Gateway destination vector

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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ppih.001.177.W133A

Plasmid

#137655

Purpose

Expresses N-terminal GST-(His)6-Thrombin cleavage site fusion of human gene or portion of gene with point mutation in bacterial strains. pET based vector.

Depositor

Tara Davis, Melissa Jurica

Article

Rajiv et al Biochem J. 2017 Oct 25;474(21):3689-3704

Insert

ppih (PPIH Human)

Tags

GST-(His)6-thrombin

Expression

Bacterial

Mutation

001-177-W133A

Available Since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-foxd4l1.1-AB1-myc

Plasmid

#185511

Purpose

Expression of Xenopus laevis foxd4

Depositor

Sally Moody

Article

Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

Insert

foxd4l1.1

Use

In vitro transcription

Tags

myc

Mutation

deletion of IDILGE in acidic blob

Available Since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-foxd4l1.1-AB1

Plasmid

#185510

Purpose

Expression of Xenopus laevis foxd4

Depositor

Sally Moody

Article

Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

Insert

foxd4l1.1

Use

In vitro transcription

Mutation

deletion of IDILGE in acidic blob

Available Since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-foxd4l1.1-deltaAB-myc

Plasmid

#185503

Purpose

Expression of Xenopus laevis foxd4

Depositor

Sally Moody

Article

Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

Insert

foxd4l1.1

Use

In vitro transcription

Tags

myc

Mutation

acidic blob (aa 21-33) deleted

Available Since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-foxd4l1.1-deltaAB

Plasmid

#185502

Purpose

Expression of Xenopus laevis foxd4

Depositor

Sally Moody

Article

Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

Insert

foxd4l1.1

Use

In vitro transcription

Mutation

acidic blob (aa 21-33) deleted

Available Since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-foxd4l1.1-QR-myc

Plasmid

#185521

Purpose

Expression of Xenopus laevis foxd4

Depositor

Sally Moody

Article

Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

Insert

foxd4l1.1

Use

In vitro transcription

Tags

myc

Mutation

replaces Q>R upstream of GARQYLNI

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-foxd4l1.1-QR

Plasmid

#185520

Purpose

Expression of Xenopus laevis foxd4

Depositor

Sally Moody

Article

Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

Insert

foxd4l1.1

Use

In vitro transcription

Mutation

replaces Q>R upstream of GARQYLNI

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2-foxd4l1.1-LA-myc

Plasmid

#185519

Purpose

Expression of Xenopus laevis foxd4

Depositor

Sally Moody

Article

Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

Insert

foxd4l1.1

Use

In vitro transcription

Tags

myc

Mutation

replaces L>A upstream of GARQYLN

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-foxd4l1.1-LA

Plasmid

#185518

Purpose

Expression of Xenopus laevis foxd4

Depositor

Sally Moody

Article

Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

Insert

foxd4l1.1

Use

In vitro transcription

Mutation

replaces L>A upstream of GARQYLNI

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-foxd4l1.1-GARP-myc

Plasmid

#185517

Purpose

Expression of Xenopus laevis foxd4

Depositor

Sally Moody

Article

Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

Insert

foxd4l1.1

Use

In vitro transcription

Tags

myc

Mutation

Replaces Q to P in GARQYNLI

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-foxd4l1.1-GARP

Plasmid

#185516

Purpose

Expression of Xenopus laevis foxd4

Depositor

Sally Moody

Article

Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

Insert

foxd4l1.1

Use

In vitro transcription

Mutation

Replaces Q to P in GARQYNLI

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-foxd4l1.1-GARG-myc

Plasmid

#185515

Purpose

Expression of Xenopus laevis foxd4

Depositor

Sally Moody

Article

Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

Insert

foxd4l1.1

Use

In vitro transcription

Tags

myc

Mutation

Replaces Q to G in GARQYNLI

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-foxd4l1.1-GARG

Plasmid

#185514

Purpose

Expression of Xenopus laevis foxd4

Depositor

Sally Moody

Article

Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

Insert

foxd4l1.1

Use

In vitro transcription

Mutation

Replaces Q to G in GARQYNLI

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-foxd4l1.1-F>E-myc

Plasmid

#185509

Purpose

Expression of Xenopus laevis foxd4

Depositor

Sally Moody

Article

Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

Insert

foxd4l1.1

Use

In vitro transcription

Tags

Myc

Mutation

F>E

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-foxd4l1.1-F>E

Plasmid

#185508

Purpose

Expression of Xenopus laevis foxd4

Depositor

Sally Moody

Article

Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

Insert

foxd4l1.1

Use

In vitro transcription

Mutation

F>E

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-foxd4l1.1-deltaRII-Cterm-myc

Plasmid

#185505

Purpose

Expression of Xenopus laevis foxd4

Depositor

Sally Moody

Article

Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

Insert

foxd4l1.1

Use

In vitro transcription

Tags

myc

Mutation

RII domain and C-term deleted

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-foxd4l1.1-deltaRII-Cterm

Plasmid

#185504

Purpose

Expression of Xenopus laevis foxd4

Depositor

Sally Moody

Article

Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

Insert

foxd4l1.1

Use

In vitro transcription

Mutation

RII domain and C-term deleted

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2-foxd4l1.1-deltaN-myc

Plasmid

#185500

Purpose

Expression of Xenopus laevis foxd4

Depositor

Sally Moody

Article

Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

Insert

foxd4l1.1

Use

In vitro transcription

Tags

myc

Mutation

N-terminus deleted

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-foxd4l1.1-deltaC-myc

Plasmid

#185501

Purpose

Expression of Xenopus laevis foxd4

Depositor

Sally Moody

Article

Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

Insert

foxd4l1.1

Use

In vitro transcription

Tags

myc

Mutation

C-terminus deleted

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2-foxd4l1.1-A6-myc

Plasmid

#185507

Purpose

Expression of Xenopus laevis foxd4

Depositor

Sally Moody

Article

Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

Insert

foxd4l1.1

Use

In vitro transcription

Tags

myc

Mutation

FSIENIM to AAAAAAM

Available Since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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pLPC-FLAG.HA.HA.Mm.RAP1.I312R

Plasmid

#184643

Purpose

Retroviral expressions of mouse RAP1 I312R

Depositor

Agnel Sfeir

Article

Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

Insert

rap1

Use

Retroviral

Tags

HA FLAG

Expression

Mammalian

Mutation

I312R

Promoter

CMV

Available Since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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pJET-10-mCherry-R11b

Plasmid

#179891

Purpose

Donor with homologous arms for Rab11b to knock in mCherry

Depositor

Patrick Caswell

Article

Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

Insert

RAB11B, member RAS oncogene family (RAB11B Human)

Use

CRISPR; Donor for homologous based knock in

Tags

mCherry

Expression

Bacterial and Mammalian

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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ppBAalbNixE1E3E4 PUb-YFP

Plasmid

#173666

Purpose

transgenesis vector for Aedes albopictus ectopic expression of the Nix gene

Depositor

Eric Marois

Article

Lutrat et al Commun Biol. 2022 Mar 7;5(1):210. doi: 1

Inserts

Nix masculinisation gene
YFP

Tags

SV40 NLS

Expression

Insect

Mutation

Nix isoforms with exons E1, E3, E4

Promoter

Aedes aegypti PUb and own

Available Since

May 11, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-HsTNRC6A_L

Plasmid

#146843

Purpose

Insect Expression of HsTNRC6A

Depositor

Elisa Izaurralde

Article

Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

Insert

HsTNRC6A (TNRC6A Human)

Expression

Insect

Mutation

A339T, K1578E compared to the sequence of isoform…

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-MS2-HA-HsNanos3-del4-20_X

Plasmid

#147945

Purpose

Mammalian Expression of HsNanos3-del4-20

Depositor

Elisa Izaurralde

Article

Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

Insert

HsNanos3-del4-20 (NANOS3 Human)

Expression

Mammalian

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pN1-HsSmg5-G120E-del133-155-V5-SBP-MBP_V

Plasmid

#147767

Purpose

Mammalian Expression of HsSmg5-G120E-del133-155

Depositor

Elisa Izaurralde

Article

Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

Insert

HsSmg5-G120E-del133-155 (SMG5 Human)

Expression

Mammalian

Mutation

one silent mutation C969T compared to the sequenc…

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pnEA-NvG-HsNot8_13-240_U

Plasmid

#147649

Purpose

Bacterial Expression of HsNot8_13-240

Depositor

Elisa Izaurralde

Article

Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

Insert

HsNot8_13-240 (CNOT8 Human)

Expression

Bacterial

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pN1-HsSmg5-G120E-siRNAres-V5-SBP-MBP_S

Plasmid

#147470

Purpose

Mammalian Expression of HsSmg5-G120E-siRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

HsSmg5-G120E-siRNAres (SMG5 Human)

Expression

Mammalian

Mutation

one silent mutation C969T compared to the sequenc…

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pTrichoGate-14

Plasmid

#160714

Purpose

For C-terminal tags with Trichoderma optimised Synthetic 3X-c-Myc tag peptide (EQKLISEEDL) bordered by BsaI restriction sites

Depositor

Artemio Mendoza

Article

Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

Insert

Trichoderma optimised Synthetic 3X-c-Myc tag peptide (EQKLISEEDL)

Use

Synthetic Biology

Available Since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pOME0136

Plasmid

#178398

Purpose

KSHV Kaposinclouster_RevCompl-KapATGdel_Syn

Depositor

Dirk Dittmer

Article

Alzhanova et al PLoS Pathog. 2021 Jan 7;17(1):e1009033.

Insert

Kaposinclouster_RevCompl-KapATGdel_Syn

Expression

Mammalian

Available Since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pOME0009R

Plasmid

#178380

Purpose

Expresses KSHV K5

Depositor

Dirk Dittmer

Article

Alzhanova et al PLoS Pathog. 2021 Jan 7;17(1):e1009033.

Insert

KSHV ORF K5

Expression

Mammalian

Available Since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pOME0013R

Plasmid

#178383

Purpose

Expresses KSHV Orf16

Depositor

Dirk Dittmer

Article

Alzhanova et al PLoS Pathog. 2021 Jan 7;17(1):e1009033.

Insert

KSHV ORF 16

Expression

Mammalian

Available Since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pOME0001R

Plasmid

#178368

Purpose

Expresses KSHV K1 stop

Depositor

Dirk Dittmer

Article

Alzhanova et al PLoS Pathog. 2021 Jan 7;17(1):e1009033.

Insert

KSHV K1

Expression

Mammalian

Available Since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pOME0007R

Plasmid

#178377

Purpose

Expresses KSHV K3

Depositor

Dirk Dittmer

Article

Alzhanova et al PLoS Pathog. 2021 Jan 7;17(1):e1009033.

Insert

KSHV ORF K3

Expression

Mammalian

Available Since

Jan. 5, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pOME0008L

Plasmid

#178379

Purpose

Expresses KSHV K3

Depositor

Dirk Dittmer

Article

Alzhanova et al PLoS Pathog. 2021 Jan 7;17(1):e1009033.

Insert

KSHV ORF K3

Use

Lentiviral

Available Since

Jan. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pOME0136MR

Plasmid

#178399

Purpose

KSHV Kaposinclouster_RevCompl-KapATGdel_Syn

Depositor

Dirk Dittmer

Article

Alzhanova et al PLoS Pathog. 2021 Jan 7;17(1):e1009033.

Insert

Kaposinclouster_RevCompl-KapATGdel_Syn

Expression

Mammalian

Available Since

Jan. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
L1-Ck1-hs1-l-chlo

Plasmid

#163937

Purpose

For integration in the chloroplast trnG_trnfM region: HS1-L, genome position: 40216_ 41843, Genbank accession no. MH635409

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

Marchantia polymorpha trnG_trnfM region left homologous sequence

Expression

Bacterial

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
L1-Ck1-hs2-l-chlo

Plasmid

#163938

Purpose

For integration in the chloroplast rbcL_trnR region: HS2-L, genome position: 56139_57662, Genbank accession no. MH635409

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

Marchantia polymorpha rbcL_trnR region homologous sequence

Expression

Bacterial

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
L1-Ck4-hs1-r-chlo

Plasmid

#163940

Purpose

For integration in the chloroplast trnG_trnfM region: HS1-R, genome position: 41847_ 43622, Genbank accession no. MH635409

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

Marchantia polymorpha trnG_trnfM region right homologous sequence

Expression

Bacterial

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
L1-Ck4-hs2-r-chlo

Plasmid

#163941

Purpose

For integration in the chloroplast rbcL_trnR region: HS2-R, genome position: 57681- 59304, Genbank accession no. MH635409

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

Marchantia polymorpha rbcL_trnR region right homologous sequence

Expression

Bacterial

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
L1_UBE2:eG-Lt-Ck4

Plasmid

#136130

Purpose

L1 in position 4, mark cell outline with eGFP

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-MpUBE2:eGFP-Lti6b_3tNos-35S

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
PROM5_MpU6

Plasmid

#136120

Purpose

Mp U6 promoter (type III RNA polymerase promoter) to drive expression of gRNA for CRISPR/Cas9

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

PROM5_MpU6

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
CDS_Cas9-NLS

Plasmid

#136121

Purpose

Puchta Arabidopsis codon-optimised Cas9 for CRISPR

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CDS_AtcoCas9-NLS

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
CDS12_Cas9-NLS

Plasmid

#136122

Purpose

Puchta Arabidopsis codon-optimised Cas9 for CRISPR. CDS12 for N-term fusion with a CTAG

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CDS12_AtcoCas9-NLS(noStop)

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
L1_NOP1:mS-N7-Ck2

Plasmid

#136126

Purpose

L1 in position 2, example of weak promoter

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-MpNOP1:mScarletI-N7_3tNos-35S

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
L1_UBE2:mT-N7-Ck2

Plasmid

#136127

Purpose

L1 in position 2, control of expression

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-MpUBE2:mTurquoise2-N7_3tNos-35S

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
L1_UBE2:mT-N7-Ck3

Plasmid

#136128

Purpose

L1 in position 3, control of expression

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-MpUBE2:mTurquoise2-N7_3tNos-35S

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
L1_UBE2:eG-Lt-Ck3

Plasmid

#136129

Purpose

L1 in position 3, mark cell outline with eGFP

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-MpUBE2:eGFP-Lti6b_3tNos-35S

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 167,150 results for: Gene