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pdCas9-J23111

Plasmid

#113149

Purpose

Plasmid constitutively expressing dCas9 protein

Depositor

Chong Zhang

Article

Wang et al Nat Commun. 2018 Jun 26;9(1):2475. doi:

Insert

dCas9

Expression

Bacterial

Mutation

D10A & H840A

Available Since

Jan. 6, 2020

Availability

Academic Institutions and Nonprofits only

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Klc1-GFP

Plasmid

#186617

Purpose

Encodes Kinesin Light Chain 1 fused to GFP

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Kinesin Light Chain 1 (KLC1 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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HBV 1.3-mer P-null replicon

Plasmid

#65462

Purpose

containing 1.3 units of the P-null HBV genome (subtype ayw)

Depositor

Wang-Shick Ryu

Article

Wang et al J Virol. 2009 Jun;83(11):5815-24. doi: 1

Insert

1.3 units of the P-null HBV genome

Expression

Mammalian

Available Since

May 29, 2015

Availability

Academic Institutions and Nonprofits only

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pX459-SpCas9-NG

Plasmid

#171370

Purpose

SpCas9-NG-mediated genome editing.

Depositor

Masahito Ikawa

Article

Oura et al Commun Biol. 2021 Jun 23;4(1):771. doi:

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6 for sgRNA; CBh for Cas9-2A-puroR

Available Since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

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sc AAV miR26a eGFP

Plasmid

#21894

Depositor

Joshua Mendell

Article

Kota et al Cell. 2009 Jun 12. 137(6):1005-17.

Insert

EF1 alpha -miR26a GFP p(A)

Use

AAV; Sc

Tags

GFP

Expression

Mammalian

Mutation

N/A

Available Since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

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C1-MPAct-mCherry

Plasmid

#155222

Purpose

Monitor changes in the density of membrane-proximal F-actin (MPA)

Depositor

Tobias Meyer

Article

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

F-tractin (aa 9-52 of rat ITPKA) fused to mCherry C-term tagged with the CaaX sequence derived from Kras4b

Tags

mCherry

Expression

Mammalian

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pMRE131

Plasmid

#118485

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mTurquoise2

Expression

Bacterial

Available Since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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SpyTag-β-Lactamase-SpyCatcher

Plasmid

#52656

Purpose

Expresses SpyTag-SpyCatcher mediated cyclized version of TEM-1 β-lactamase (BLA). Cyclization of BLA led to an increase in thermal resilience of >60 degrees celsius.

Depositor

Mark Howarth

Article

Schoene et al Angew Chem Int Ed Engl. 2014 Jun 10;53(2

Insert

TEM-1 β-Lactamase

Tags

SpyCatcher and SpyTag

Expression

Bacterial

Promoter

T7 promoter

Available Since

May 1, 2014

Availability

Academic Institutions and Nonprofits only

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pMJ529: pU6-SpuFz1_MS2-omegaRNA(75nt)-Guide(B2M)

Plasmid

#205278

Purpose

Human expression vector for SpuFz1 omegaRNA 75-nt scaffold + 5' MS2- (Guide:B2M)

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

SpuFz1 omegaRNA

Expression

Mammalian

Available Since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-Ef1a-InteinC-CreC

Plasmid

#187615

Purpose

AAV vector encoding C-terminal portion of split-Intein-mediated split-Cre

Depositor

Il Hwan Kim

Article

Kim et al Cell Rep. 2022 Jun 7;39(10):110906. doi:

Insert

InteinC-CreC

Use

AAV

Expression

Mammalian

Available Since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-TRE-tdKatushka2-WPRE

Plasmid

#193337

Purpose

Expresses tdKatushka2 under the TRE promoter in mammalian cells

Depositor

Takeshi Imai

Article

Leiwe et al Nat Commun. 2024 Jun 25;15(1):5279. doi:

Insert

tdKatushka2

Expression

Mammalian

Promoter

TRE

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV SOCS-3

Plasmid

#11486

Depositor

Ronald Kahn

Article

Ueki et al Mol Cell Biol. 2004 Jun . 24(12):5434-46

Insert

SOCS-3 (Socs3 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

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pCAFNF-ECFP-Actb

Plasmid

#169787

Purpose

An actin FRET sensor. Must be expressed with Venus-actin for FRET.

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Actb (Actb Mouse)

Tags

ECFP

Expression

Mammalian

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST-hisMBP-AsCpf1-EC

Plasmid

#79007

Purpose

Expressing his-MBP tagged AsCpf1 (E.coli codon optimized)

Depositor

Jin-Soo Kim

Article

Hur et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

Insert

AsCpf1

Tags

6xHis-MBP-NLS and NLS-HA

Expression

Bacterial

Promoter

tac promoter

Available Since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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pSF11-wmTagBFP2

Plasmid

#184934

Purpose

Codon optimized blue fluorescent protein mTagBFP2 in C.elegans expresison plasmid

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

mTagBFP2

Expression

Worm

Promoter

tag-168

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-Ef1a-CreN-InteinN

Plasmid

#187614

Purpose

AAV vector that encodes N-terminal portion of split-Intein-mediated split-Cre

Depositor

Il Hwan Kim

Article

Kim et al Cell Rep. 2022 Jun 7;39(10):110906. doi:

Insert

CreN-InteinN

Use

AAV

Expression

Mammalian

Available Since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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pMGE-T7

Plasmid

#153021

Purpose

Single-plasmid multi-gene expression system. Each genes under the control of a T7 and the lacO promoter sequences.

Depositor

Vito Valiante

Article

Kufs et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

Type

Empty backbone

Expression

Bacterial

Promoter

T7

Available Since

June 23, 2020

Availability

Academic Institutions and Nonprofits only

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EGFP-mCherry-Ki67

Plasmid

#183750

Purpose

Expresses EGFP-mCherry-Ki67

Depositor

Daniel Gerlich

Article

Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

Insert

MKI67 (MKI67 Human)

Tags

EGFP and mCherry

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-PIK3C2B

Plasmid

#161989

Purpose

Mammalian expression vector containing PIK3C2B tagged with GFP

Depositor

David Bryant, Volker Haucke

Article

Marat et al Science. 2017 Jun 2;356(6341):968-972. d

Insert

PIK3C2B (PIK3C2B Human)

Tags

GFP

Expression

Mammalian

Mutation

siRNA resistance to siRNA#2 with 4 silent mutatio…

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pMRE132

Plasmid

#118486

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

sGFP2

Expression

Bacterial

Available Since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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