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pMaCTag-P34

Plasmid

#120030

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 3xFLAG

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

3xFLAG

Use

Pcr template

Tags

3xFLAG

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pTubulin-mTagBFP2

Plasmid

#184931

Purpose

Tubulin labeling with blue fluorescent protein mTagBFP2

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

TUBA1B-mTagBFP2

Tags

mTagBFP2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-HA-hTRAF3

Plasmid

#44032

Depositor

Shao-Cong Sun

Article

Liao et al J Biol Chem. 2004 Jun 18;279(25):26243-5

Insert

TNF receptor-associated factor 3 (TRAF3 Human)

Tags

1xHA

Expression

Mammalian

Mutation

S393G

Available Since

April 4, 2013

Availability

Academic Institutions and Nonprofits only
pMJA179

Plasmid

#128537

Purpose

Expresses anti-Prunus dulcis scFv Ab

Depositor

Marcos Alcocer

Article

de la Cruz et al J Biotechnol. 2016 Jun 10;227:112-9. doi

Insert

scFv Ab clone PD1F6

Expression

Yeast

Promoter

AOX1

Available Since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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PX552-mScn2a-3xV5-EF1-smFP-flag

Plasmid

#182561

Purpose

For mouse Nav1.2 knockin with 3xV5 at the C-terminal

Depositor

James Zhe Liu

Article

Liu et al J Neurosci. 2022 Jun 6;42(28):5482-98. d

Inserts

mouse Scn2a gRNA and 3xV5 donor DNA
smFP-flag

Use

AAV and CRISPR

Promoter

EF1 and U6

Available Since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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pMRE137

Plasmid

#118491

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mClover3

Expression

Bacterial

Available Since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pBS-TRE-tdKatushka2-WPRE

Plasmid

#193337

Purpose

Expresses tdKatushka2 under the TRE promoter in mammalian cells

Depositor

Takeshi Imai

Article

Leiwe et al Nat Commun. 2024 Jun 25;15(1):5279. doi:

Insert

tdKatushka2

Expression

Mammalian

Promoter

TRE

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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TB:7xE-Box:MiniP

Plasmid

#133880

Purpose

7 copies of the E-box motif

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

Insert

7 copies of the E-box motif

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Available Since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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pRJPaph-bjGFP

Plasmid

#67032

Purpose

Plasmid for Paph-bjGFP integration downstream of B. diazoefficiens (B. japonicum) scoI

Depositor

Julia Vorholt

Article

Ledermann et al Mol Plant Microbe Interact. 2015 Jun 2.

Inserts

B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
bjGFP

Use

Conjugative bacterial vector

Available Since

Sept. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV5 Flag-Smad1

Plasmid

#14044

Depositor

Joan Massague

Article

Liu et al Nature. 1996 Jun 13. 381(6583):620-3.

Insert

Smad1 (SMAD1 Human)

Tags

Flag

Expression

Mammalian

Available Since

May 10, 2007

Availability

Academic Institutions and Nonprofits only

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pLVX-IRES-Gβγ-BERKY3

Plasmid

#158426

Purpose

BRET biosensor for detection of free Gβγ in lentiviral vector pLVX-IRES-Hyg

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

Lyn11-Nluc-ER/K linker-3xYFP-GRK3ct

Use

Lentiviral

Promoter

CMV

Available Since

Sept. 17, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pMaCTag-P28

Plasmid

#120039

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 3xHA

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

3xHA

Use

Pcr template

Tags

3xHA

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMRE133

Plasmid

#118487

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

sYFP2

Expression

Bacterial

Available Since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pMRE162

Plasmid

#118510

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

sGFP2

Expression

Bacterial

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pMRE130

Plasmid

#118484

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

TagBFP2

Expression

Bacterial

Available Since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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TB:6xAP-1_RE:MiniP

Plasmid

#133884

Purpose

6 copies of the AP-1 binding motif

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

Insert

6 copies of the AP-1 binding motif

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Available Since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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pegfp-N1-TFEB-S3A,R4A

Plasmid

#44446

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

Insert

TFEB (TFEB Human)

Tags

GFP

Expression

Mammalian

Mutation

Changed both Serine 3 and Arginine 4 to Alanine

Promoter

CMV

Available Since

April 26, 2013

Availability

Academic Institutions and Nonprofits only

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pMSC234

Plasmid

#216357

Purpose

xhNup93-Nb3t (+2Cys) Nanobody production

Depositor

Dirk Görlich

Article

Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

Insert

xhNup93-Nb3t (+2Cys)

Use

Affinity Reagent/ Antibody

Tags

14xHis-NEDD8

Expression

Bacterial

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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AviTag-SpyTag003-MBP

Plasmid

#184227

Purpose

Expresses AviTag and SpyTag003 linked to maltose binding protein in the bacterial cytoplasm. AviTag enables site-specific biotinylation by BirA. SpyTag003 enables reaction with SpyCatcher003.

Depositor

Mark Howarth

Article

Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:

Insert

AviTag-SpyTag003-MBP

Tags

AviTag, His6, SpyTag003, and Thrombin cleavage si…

Expression

Bacterial

Promoter

T7

Available Since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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ECFP-Bak

Plasmid

#31501

Depositor

Richard Youle

Article

Nechushtan et al J Cell Biol. 2001 Jun 11. 153(6):1265-76

Insert

human Bak (BAK1 Human)

Tags

ECFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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