We narrowed to 14,039 results for: TIM

1. CRY2olig-mCh-Nck

   Plasmid

   #60034

   Purpose

   Expresses a fusion containing CRY2olig (PHR, E490G) with mCherry and 3 SH3 domains of Nck

   Depositor

   Chandra Tucker

   Article

   Taslimi et al Nat Commun. 2014 Sep 18;5:4925. doi: 10.

   Insert

   CRY2-mCh-NCK (CRY2 Mustard Weed, Human)
   

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   M354I, E490G CRY2

   Promoter

   CMV

   Available Since

   Nov. 12, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-T7-AsCas12a-P2A-EGFP (RTW2861)

   Plasmid

   #160140

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized AsCas12a with a c-terminal NLS(nucleoplasmin), 3x HA tag, and P2A-EGFP

   Depositor

   Keith Joung, Benjamin Kleinstiver

   Article

   Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

   Insert

   human codon optimized AsCas12a with NLS(nucleoplasmin)-3xHA-P2A-EGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   NLS(nucleoplasmin)-3xHA-P2A-EGFP

   Expression

   Mammalian

   Promoter

   CMV and T7

   Available Since

   Feb. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pENN.AAV.GFAP.iGluSnFr.WPRE.SV40

   Plasmid

   #98930

   Purpose

   AAV expression of glutamate sensor from GFAP promoter. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:

   Has Service

   AAV1, AAV5, and AAV9

   Insert

   iGluSnFr
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   GFAP

   Available Since

   Jan. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.2/NS5-YFP-NLS-mut

   Plasmid

   #120832

   Purpose

   Ectopic expression of ZIKV protein

   Depositor

   Jan Rehwinkel

   Article

   Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

   Insert

   ZIKV NS5-eYFP "NLS-mut"
   

   Tags

   eYFP-V5

   Expression

   Mammalian

   Mutation

   R372A/Q373A/K387A/H388A/K389A

   Promoter

   CMV

   Available Since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. non-targeting control gRNA (BRDN0001148500)

   Plasmid

   #80260

   Purpose

   3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   Non-targeting control gRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Aug. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEB2-mRuby3 Addgene

   Plasmid

   #104005

   Purpose

   Plasmid encoding mRuby3 Addgene

   Depositor

   Philippe Cluzel

   Article

   Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

   Insert

   mRuby3 Addgene
   

   Use

   Low copy

   Expression

   Bacterial

   Mutation

   Mutations relative to eqFP611 (MSKGEE LIKENMRMKVV…

   Promoter

   proC

   Available Since

   Dec. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYNL-C1_Rab11a

   Plasmid

   #65705

   Purpose

   Expresses YNL-Rab11a in mammalian cells

   Depositor

   Yasushi Okada

   Article

   Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   Rab11a (Rab11a Mouse)
   

   Use

   Luciferase

   Tags

   YNL

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. NEK7 gRNA (BRDN0001162196)

   Plasmid

   #77740

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human NEK7

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   NEK7 (NEK7 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSUPER-retro-puro-shOrai1

   Plasmid

   #89933

   Purpose

   Knockdown human Orai1 expression

   Depositor

   Shengyu Yang

   Article

   Sun et al J Cell Biol. 2014 Nov 24;207(4):535-48.

   Insert

   Orai1 shRNA (ORAI1 Human)
   

   Use

   Retroviral

   Available Since

   May 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1(+)- e18 LOF

    Plasmid

    #131729

    Purpose

    Expresses a loss of function mutant of an engineered variant of RAD18 ("e18")

    Depositor

    Alberto Ciccia

    Article

    Nambiar et al Nat Commun. 2019 Jul 30;10(1):3395. doi:

    Insert

    e18/D221A (Loss of function mutant of RAD18 with SAP domain deleted and UBZ point mutation) (RAD18 Human)
    

    Tags

    FLAG and HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. SspB-mCherry-DHPH-TIAM1

    Plasmid

    #176104

    Purpose

    Heterodimerization with iLID, Rac1 activation

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

    Insert

    TIAM1 (TIAM1 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. PIK3C2B gRNA (BRDN0001145952)

    Plasmid

    #76714

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PIK3C2B

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PIK3C2B (PIK3C2B Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. RV-Cag-Dio-EYFP-P2A-cMaf

    Plasmid

    #98174

    Purpose

    Retroviral vector encoding Cre-dependent expression of EYFP-P2A-Maf

    Depositor

    Oscar Marin

    Article

    Mi et al Science. 2018 Feb 22. pii: science.aar68

    Insert

    cMaf (codon optimzed) (Maf Mouse)
    

    Use

    Retroviral

    Mutation

    Codon optimized

    Promoter

    CAG

    Available Since

    July 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEB2-mRuby3

    Plasmid

    #104004

    Purpose

    Plasmid encoding mRuby3

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    mRuby3
    

    Use

    Low copy

    Expression

    Bacterial

    Mutation

    Mutations relative to eqFP611 (MSKGEE LIKENMRMKVV…

    Promoter

    proC

    Available Since

    Dec. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. TK1 gRNA (BRDN0001145069)

    Plasmid

    #76424

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TK1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    TK1 (TK1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. WDR5-HaloTag

    Plasmid

    #200881

    Purpose

    Mammalian expression of human WDR5 with C-terminal HaloTag fusion

    Depositor

    Stefan Knapp

    Article

    Schwalm et al Cell Chem Biol. 2023 Jun 20:S2451-9456(2

    Insert

    WDR5-HaloTag (WDR5 Human)
    

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-hCjeCas9-NLS(SV40)-3xFLAG (KAC579)

    Plasmid

    #133789

    Purpose

    CAG promoter expression plasmid for human codon optimized CjeCas9 nuclease with C-terminal NLS (SV40)

    Depositor

    Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    human codon optimized CjeCas9
    

    Tags

    NLS(SV40)-3xFLAG

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    CAG

    Available Since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pENTR4-EGFP_opt

    Plasmid

    #106334

    Purpose

    Gateway Entry vector for EGFP (codon optimized for efficient gene synthesis)

    Depositor

    Thomas Tuschl

    Article

    Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

    Insert

    EGFP
    

    Use

    Gateway entry vector

    Expression

    Bacterial and Mammalian

    Mutation

    codon optimized for gene synthesis without affect…

    Available Since

    March 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. NUAK1 gRNA (BRDN0001162598)

    Plasmid

    #75577

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human NUAK1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    NUAK1 (NUAK1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28a(ICOS)

    Plasmid

    #177903

    Purpose

    Bacterial expression of his10-tagged human ICOS Intracellular Domain

    Depositor

    Ron Vale

    Article

    Hui et al Science. 2017 Mar 31;355(6332):1428-1433

    Insert

    ICOS (aa 162-199 only) (ICOS Human)
    

    Tags

    10xHis-Thrombin cut site-T7 tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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