We narrowed to 19,149 results for: Tec;

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FUGW-d2GFP-ZEB1 3'UTR

Plasmid

#79601

Purpose

FUGW lentiviral vector with constitutively expressed destabilized GFP (d2GFP) regulated by the human ZEB1 3'UTR. When used in combination with Ecad-RFP, comprises the Z-cad dual sensor.

Depositor

Jeffrey Rosen

Article

Toneff et al BMC Biol. 2016 Jun 17;14(1):47.

Insert

d2GFP-Zeb1 3'UTR

Use

Lentiviral

Available Since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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pAF-HdrB3

Plasmid

#184908

Purpose

carrying CRISPR/Cas9 system for genome editing in Acidithiobacillus ferridurans

Depositor

Radhakrishnan Mahadevan

Article

Chen et al Environ Sci Technol. 2023 Aug 22;57(33):

Insert

Cas9-sgRNA-homology arms

Use

CRISPR; Broad-host-range

Expression

Bacterial

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-KG/Net1

Plasmid

#28260

Depositor

Jeffrey Frost

Article

Carr et al J Biol Chem. 2009 Sep 4. 284(36):24269-8

Insert

Net1 (Net1 Mouse)

Tags

GST

Expression

Bacterial

Available Since

April 11, 2011

Availability

Academic Institutions and Nonprofits only
FokI-dCas9

Plasmid

#52970

Purpose

Expresses Fok1 nuclease domain fused to catalytically inactive Cas9 DNA-binding domain in mammalian cells

Depositor

David Liu

Article

Guilinger et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

Insert

Fok1 fused to dCas9

Use

CRISPR

Tags

Fok1 nuclease (homodimeric wildtype) and NLS

Expression

Mammalian

Mutation

D10A and H840A in Cas9

Promoter

CMV

Available Since

May 21, 2014

Availability

Academic Institutions and Nonprofits only

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pMM114

Plasmid

#127211

Purpose

BeYDV replicon with constitutive Luc expression, WUS2 and IPT, sgRNA targeting PDS

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

WUS2, IPT, sgRNA, Luc

Expression

Plant

Mutation

codon optimized CDSs

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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TurboID-V5_pRS415

Plasmid

#107167

Purpose

expresses V5-tagged TurboID in the yeast cytosol

Depositor

Alice Ting

Article

Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

Insert

TurboID (BirA mutant)

Expression

Yeast

Mutation

Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

Available Since

May 4, 2018

Availability

Academic Institutions and Nonprofits only

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pLX_311-Cas9

Plasmid

#96924

Purpose

for Cas9 knockout, lentiviral expression of Cas9. Alternate plasmid name: pXR111

Depositor

John Doench, William Hahn, David Root

Article

Doench et al Nat Biotechnol. 2014 Sep 3. doi: 10.1038

Insert

Cas9

Use

Lentiviral

Tags

3xFlag and V5

Expression

Mammalian

Promoter

EF1a

Available Since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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pGWB508-cTP-mCherry

Plasmid

#213862

Purpose

Binary vector for the expression of mCherry flanked with an N-terminal plastid transit peptide in plants (for Agrobacterium-mediated genetic transformation and visualization of plastid.)

Depositor

Yutaka Kodama

Article

Ishikawa et al Plant Cell Rep. 2023 Mar;42(3):599-607.

Insert

Plastid transit peptide fused mCherry-UnaG

Expression

Bacterial and Plant

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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BPK2660

Plasmid

#70709

Purpose

Human expression plasmid for SaCas9 sgRNA(84nt in length) (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sa-sgRNA(84)

Depositor

Keith Joung

Article

Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pXPR_011

Plasmid

#59702

Purpose

This lentiviral vector can be used to assay Cas9 activity.

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2014 Sep 3. doi: 10.1038

Insert

EGFP sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Sept. 3, 2014

Availability

Academic Institutions and Nonprofits only

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35S-GAL4BD-GWY/pAM

Plasmid

#201233

Purpose

Used to produce and express in planta N-terminal fusion proteins with the GAL4 BD

Depositor

Michael Ayliffe

Article

Chen et al Plant J. 2023 Jun;114(6):1209-1226. doi:

Insert

35S-GAL4BD-GWY

Expression

Plant

Promoter

CaMV 35S

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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35S-GWY-GAL4BD/pAM

Plasmid

#201231

Purpose

Used to produce and express in planta N-terminal fusion proteins with the GAL4 BD

Depositor

Michael Ayliffe

Article

Chen et al Plant J. 2023 Jun;114(6):1209-1226. doi:

Insert

35S-GWY-GAL4BD

Expression

Plant

Promoter

CaMV 35S

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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3xHA-miniTurbo-NLS_pCDNA3

Plasmid

#107172

Purpose

expresses 3xHA-tagged miniTurbo in the mammalian nucleus

Depositor

Alice Ting

Article

Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

Insert

miniTurbo (BirA mutant)

Expression

Mammalian

Mutation

aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

Available Since

May 4, 2018

Availability

Academic Institutions and Nonprofits only

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pBT29-splitD

Plasmid

#122599

Purpose

clone M13 phage by SapI Golden Gate

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

M13 bacteriophage genes

Expression

Bacterial

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pBT29-splitC

Plasmid

#122598

Purpose

clone M13 phage by SapI Golden Gate

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

M13 bacteriophage genes

Expression

Bacterial

Available Since

Aug. 21, 2019

Availability

Academic Institutions and Nonprofits only

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tdTomato-N1

Plasmid

#54642

Purpose

Localization: N1 Cloning Vector, Excitation: 554, Emission: 581

Depositor

Michael Davidson, Nathan Shaner, Roger Tsien

Article

Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

Has Service

Cloning Grade DNA

Type

Empty backbone

Tags

tdTomato

Expression

Mammalian

Promoter

CMV

Available Since

June 20, 2014

Availability

Academic Institutions and Nonprofits only

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COM-epegRNA-Dnmt1

Plasmid

#211376

Purpose

Dnmt1 targeting epegRNA with COM insertion in gRNA scaffold for v3b PE-eVLP production

Depositor

David Liu

Article

An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

Insert

COM-epegRNA

Expression

Mammalian

Available Since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pBT137-splitC

Plasmid

#122605

Purpose

clone M13 phage by SapI Golden Gate

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

M13 bacteriophage genes

Expression

Bacterial

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pBT137-splitD

Plasmid

#122606

Purpose

clone M13 phage by SapI Golden Gate

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

M13 bacteriophage genes

Expression

Bacterial

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pXPR_206

Plasmid

#96920

Purpose

for SaCas9 knockout, lentiviral expression of S. aureus Cas9 (SauCas9) and gRNA scaffold

Depositor

John Doench, David Root

Article

Najm et al Nat Biotechnol. 2018 Feb;36(2):179-189.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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