We narrowed to 23,593 results for: promoter

1. pUC-hU6-crScaffold_SV40-BFP

   Plasmid

   #224860

   Purpose

   crRNA scaffold for RfxCas13d expressed from hU6 promoter and reporter BFP protein expressed from SV40 promoter

   Depositor

   Gabor Balazsi

   Article

   Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Oct. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRK-flag-USP19 C506S

   Plasmid

   #78584

   Purpose

   Expression flag-USP19 C506S in mammalian cells

   Depositor

   Yihong Ye

   Article

   Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

   Insert

   flag-USP19 C506S (USP19 Human)
   

   Tags

   flag

   Expression

   Mammalian

   Mutation

   mutate 506 cysteine residue to serine

   Promoter

   CMV

   Available Since

   June 22, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pGEX-p53 R175H

   Plasmid

   #39480

   Depositor

   Ie-Ming Shih

   Article

   Guan et al Cancer Res. 2011 Nov 1;71(21):6718-27. E

   Insert

   TP53 (TP53 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   P72R and R175H

   Available Since

   Sept. 25, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pLPP3

   Plasmid

   #209965

   Purpose

   Contains Level 0 Part: constitutive Promoter (P25) for the construction of Level 1 plasmids

   Depositor

   Thorsten Mascher

   Article

   Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

   Insert

   Promoter (P25)
   

   Expression

   Bacterial

   Available Since

   Sept. 13, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pLPP7

   Plasmid

   #209969

   Purpose

   Contains Level 0 Part: autoinducible Promoter (PfabZ) for the construction of Level 1 plasmids

   Depositor

   Thorsten Mascher

   Article

   Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

   Insert

   Promoter (P_fabZ)
   

   Expression

   Bacterial

   Available Since

   Sept. 13, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pLPP8

   Plasmid

   #209970

   Purpose

   Contains Level 0 Part: autoinducible Promoter (PgadB) for the construction of Level 1 plasmids

   Depositor

   Thorsten Mascher

   Article

   Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

   Insert

   Promoter (P_gadB)
   

   Expression

   Bacterial

   Available Since

   Sept. 13, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pLPP9

   Plasmid

   #209971

   Purpose

   Contains Level 0 Part: constitutive Promoter (PldhD) for the construction of Level 1 plasmids

   Depositor

   Thorsten Mascher

   Article

   Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

   Insert

   Promoter (P_ldhD)
   

   Expression

   Bacterial

   Available Since

   Sept. 13, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pLPP1

   Plasmid

   #209963

   Purpose

   Contains Level 0 Part: mock / negative control Promoter (P-nc) for the construction of Level 1 plasmids

   Depositor

   Thorsten Mascher

   Article

   Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

   Insert

   Promoter (mock)
   

   Expression

   Bacterial

   Available Since

   Sept. 13, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pLPP2

   Plasmid

   #209964

   Purpose

   Contains Level 0 Part: constitutive Promoter (P48) for the construction of Level 1 plasmids

   Depositor

   Thorsten Mascher

   Article

   Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

   Insert

   Promoter (P48)
   

   Expression

   Bacterial

   Available Since

   Sept. 13, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pXPR_212

    Plasmid

    #133457

    Purpose

    U6 promoter expresses customizable Spyo-guide; EFS promoter expresses SaurCas9 and 2A site provides puromycin resistance

    Depositor

    John Doench

    Article

    DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1

    Insert

    control guide
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEX-p53

    Plasmid

    #39479

    Depositor

    Ie-Ming Shih

    Article

    Guan et al Cancer Res. 2011 Nov 1;71(21):6718-27. E

    Insert

    TP53 (TP53 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    P72R

    Available Since

    Sept. 25, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pXPR_213

    Plasmid

    #133456

    Purpose

    H1 promoter expresses customizable Saur-guide; EF1a promoter expresses SpyoCas9 and 2A site provides blasticidin resistance

    Depositor

    John Doench

    Article

    DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1

    Insert

    Control guide
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. USP19 sgRNA2

    Plasmid

    #78586

    Purpose

    delete USP19 gene in human cell

    Depositor

    Yihong Ye

    Article

    Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

    Insert

    USP19 sgRNA2 (USP19 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only

14. 1456_pDEST_miniTol2_R4-R2_Cryst-eGFP

    Plasmid

    #171795

    Purpose

    pDEST miniTol2-clone for R4-R2 3-component gateway assembly (p5E + pME). Include a eGFP selection marker (Crystallin promoter Lens/Eyes)

    Depositor

    Jean Giacomotto

    Article

    Tromp et al Front Physiol. 2023 Aug 2;14:1221310. do

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pT7-AsCas12a_crRNA-site4 (MSP3511)

    Plasmid

    #160139

    Purpose

    T7 promoter expression plasmid for in vitro transcription of AsCas12a crRNA with spacer #4

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

    Insert

    AsCas12a crRNA with spacer #4 (spacer=GGAATCCCTTCTGCAGCACCTGG)
    

    Use

    In vitro transcription of sgrna from t7 promoter

    Promoter

    T7

    Available Since

    Feb. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pENTR_hEMSY

    Plasmid

    #125156

    Purpose

    Gateway entry clone

    Depositor

    Alexander Stark

    Article

    Haberle et al Nature. 2019 Jun;570(7759):122-126. doi:

    Insert

    EMSY (EMSY Human)
    

    Use

    Gateway entry vector

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEBG-TFII-I delta isoform

    Plasmid

    #22188

    Depositor

    Ananda Roy

    Article

    Cheriyath et al Mol Cell Biol. 1998 Aug . 18(8):4444-54.

    Insert

    TFII-I (GTF2I Human)
    

    Tags

    GST and His

    Expression

    Mammalian

    Available Since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLV-SFFV-RAB10 Q68L-WPRE-UbC-Emerald

    Plasmid

    #203803

    Purpose

    Lentiviral vector plasmid expressing human RAB10 mutation Q68L (constitutively active mutant) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

    Depositor

    Bart Nieuwenhuis, Richard Eva

    Inserts

    RAB10 (RAB10 Human)
    Emerald
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Q68L

    Available Since

    July 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-nDsRedFL-miR9/9*

    Plasmid

    #177805

    Purpose

    The pre-miR-9/9* sequence has been inserted into the EcoRV of pCAG-nDsRedFL-intron.

    Depositor

    Rikako Sanuki

    Article

    Suzuki et al Genes Cells. 2020 Nov;25(11):741-752. do

    Insert

    miR-9/9*
    

    Expression

    Mammalian

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEGFP-C1 beta-catenin Y654E

    Plasmid

    #16073

    Depositor

    Erin Schuman

    Article

    Murase et al Neuron. 2002 Jul 3. 35(1):91-105.

    Insert

    beta-catenin (CTNNB1 Chicken)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Y654E, *(see below)

    Available Since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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