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FLT4 gRNA (BRDN0001147213)

Plasmid

#75630

Purpose

3rd generation lentiviral gRNA plasmid targeting human FLT4

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

FLT4 (FLT4 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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FLT4 gRNA (BRDN0001149037)

Plasmid

#75632

Purpose

3rd generation lentiviral gRNA plasmid targeting human FLT4

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

FLT4 (FLT4 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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DLG1 gRNA (BRDN0001147622)

Plasmid

#76620

Purpose

3rd generation lentiviral gRNA plasmid targeting human DLG1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

DLG1 (DLG1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

June 27, 2016

Availability

Academic Institutions and Nonprofits only

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CSNK1D gRNA (BRDN0001146126)

Plasmid

#76715

Purpose

3rd generation lentiviral gRNA plasmid targeting human CSNK1D

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CSNK1D (CSNK1D Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

June 27, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR223_DKK1_WT

Plasmid

#82250

Purpose

Gateway Donor vector containing DKK1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

DKK1 (DKK1 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a-AlkB

Plasmid

#228218

Purpose

Express wild type AlkB protein in E. coli BL21(DE3)

Depositor

Junchao Shi

Article

Shi et al Nat Cell Biol. 2021 Apr;23(4):424-436. d

Insert

Alpha-ketoglutarate-dependent Dioxygenase (alkB Synthetic)

Tags

His tag

Expression

Bacterial

Mutation

codon optimized

Promoter

T7

Available Since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pUC57-2xFLAG-mAID-AMBRA1 HDR donor template

Plasmid

#172609

Purpose

Donor plasmid containing the HDR template for the in-frame, N-terminal 2xFLAG-mAID tagging of AMBRA1 gene

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

2xFLAG-mAID-AMBRA1 (AMBRA1 Human, Synthetic)

Use

CRISPR; Human targeting

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_CBFA2T3_GLIS2

Plasmid

#205786

Purpose

Express mEGFP-tagged fusion protein, CBFA2T3_GLIS2 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

CBFA2T3_GLIS2 (CBFA2T3, GLIS2 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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SMARCA2-GFP

Plasmid

#65390

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

SMARCA2 (SMARCA2 Human)

Tags

EmGFP

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

Oct. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pF1-Hs Cdk1/cyclin B1

Plasmid

#177330

Purpose

Co-expression of the human active Cdk1/cyclin B1 kinase complex

Depositor

Andreas Boland

Article

Yu et al Nature. 2021 Aug;596(7870):138-142. doi:

Inserts

Cyclin-dependent kinase 1 (CDK1 Human)
G2/mitotic-specific cyclin-B1 (CCNB1 Human)

Tags

2 x Strep-tag and 8 x His

Expression

Insect

Available Since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223_GPS2_WT

Plasmid

#81816

Purpose

Gateway Donor vector containing GPS2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

GPS2 (GPS2 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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TFORF0381

Plasmid

#142256

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

SMARCA2 (SMARCA2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-DIO-HA-HCARD-P2A-mCitrine

Plasmid

#228902

Purpose

For AAV packaging and Cre-dependent expression of a peripherally restricted DREADD

Depositor

Bryan Roth

Article

Kang et al Cell. 2024 Dec 26;187(26):7433-7449.e20.

Has Service

AAV8

Insert

hydroxycarboxylic acid receptor 2 (Hcar2 Mouse)

Use

AAV and Synthetic Biology

Tags

HA and P2A mCitrine

Expression

Mammalian

Mutation

R108K

Promoter

Chicken Beta Actin

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pSAD-5PSD95-EGFP-SynPhRFP

Plasmid

#217979

Purpose

G-Deleted Rabies genomic plasmid to express 5PSD95-EGFP and SynPhRFP

Depositor

Edward Callaway

Article

Patino et al Elife. 2023 Dec 14;12:e89297. doi: 10.75

Inserts

5PSD95-EGFP (Dlg4 Rat)
SynPhRFP (Syp Mouse)

Use

G-deleted rabies

Tags

EGFP and RFP

Promoter

None

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-Blast POLE1

Plasmid

#160802

Purpose

Express POLE1

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

POLE1 (POLE Human)

Use

Retroviral

Tags

3xFLAG

Mutation

shPOLE_1 and shPOLE2 resistant

Available Since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1 PA-PLA1 (EGFP-PA-PLA1)

Plasmid

#162880

Purpose

Expression in mammalian cells of Phosphatidic Acid preferring Phospholipase A1 tagged with EGFP

Depositor

Frederic Meunier

Article

Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p

Insert

Phosphatidic acid-preferring phospholipase A1 (PA-PLA1) (DDHD1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-Blast POLE1 N-ISCmut

Plasmid

#160803

Purpose

Express N-terminal ISC mutant POLE1

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

POLE1 N-ISCmut (POLE Human)

Use

Retroviral

Tags

3xFLAG

Mutation

shPOLE_1 and shPOLE2 resistant, C651S, C654S, C66…

Available Since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pRetroX-TetOne-Puro-p62

Plasmid

#204548

Purpose

Expresses p62 in a doxycycline-dependent manner in mammalian cells

Depositor

Masaaki Komatsu

Article

Kurusu et al Dev Cell. 2023 May 9:S1534-5807(23)00191

Insert

p62 (SQSTM1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

None

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pTT3-ecdICAM1-ST3-His

Plasmid

#210666

Purpose

Expression of the extracellular domain of human ICAM1 fused to Spytag003 + Histag in HEK293 (or similar)

Depositor

Omer Dushek

Article

Patel et al EMBO J. 2024 Jan;43(1):132-150. doi: 10.

Insert

Extracellular domain of human ICAM-1 fused to Spytag003 and Histag (ICAM1 Human, Synthetic)

Tags

Histag and Spytag003

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST-Myc-FBW7 WD40 (All deleted)

Plasmid

#197455

Purpose

The plasmid expresses delta WD40 deleted version of Myc-tagged FBW7 human isoform 1. WD40 All are deleted. Used for mammalian overexpression and detection by western blotting.

Depositor

Omar Khan

Article

Khan et al Nat Commun. 2021 Apr 6;12(1):2043. doi:

Insert

FBW7 (FBXW7 Human)

Tags

Myc

Expression

Mammalian

Mutation

FBW& dimerization domain deleted.

Promoter

CMV

Available Since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

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