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pCDNA5/FRT/TO_HaloTag7_T2A_EGFP

Plasmid

#169325

Purpose

Expression of HaloTag7 in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Type

Empty backbone

Expression

Mammalian

Available Since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pAF0362 Ef1a-Cp36_attP-Puro-p2a-mCherry donor

Plasmid

#193469

Purpose

Cp36 Donor with promoter for mcherry and puro expression

Depositor

Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

Cp36_attP

Expression

Mammalian

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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phCMV-229E-D15

Plasmid

#188908

Purpose

express truncated 229E spike for pseudovirus production

Depositor

David Nemazee

Insert

229E spike (S 229E)

Expression

Mammalian

Mutation

c-terminal 15aa deletion

Promoter

CMV

Available Since

Aug. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-EGFR-V5/HIS

Plasmid

#201102

Purpose

expression of human EGFR receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

human EGFR receptor tyrosine kinase, full length, wildtype (EGFR Human)

Tags

V5/His

Expression

Mammalian

Promoter

CMV

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pCXLE-Sox2AV-T2A-Klf4-E2A-Myc

Plasmid

#210017

Purpose

episomal SKM (expressing mouse Sox2-A61V + Klf4 + Myc) for integration-free naïve reset.

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

Sox2AV-t2a-Klf4-e2a-cMyc (Klf4, Sox2 Mouse)

Expression

Mammalian

Mutation

Sox2A61V is a point mutant of Sox2 that enhances …

Promoter

CAG

Available Since

Dec. 14, 2023

Availability

Academic Institutions and Nonprofits only

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human EB1 in pEGFP N1 to express EB1-GFP (JB131)

Plasmid

#39299

Depositor

Tim Mitchison, Jennifer Tirnauer

Insert

EB1 (MAPRE1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only

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pCMV-RdLight1

Plasmid

#125706

Purpose

Expresses the red genetically encoded dopamine sensor RdLight1 in mammalian cells.

Depositor

Lin Tian

Article

Patriarchi et al Nat Methods. 2020 Nov;17(11):1147-1155.

Insert

RdLight1

Tags

Flag tag

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-jGCaMP8s-WPRE

Plasmid

#179256

Purpose

AAV-mediated expression of GCaMP8s under the CAG promoter

Depositor

Loren Looger

Has Service

AAV Retrograde, AAV1, and AAV9

Insert

jGCaMP8s

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCDH-CMV-V5-RFP-LATS1

Plasmid

#235689

Purpose

Express RFP tagged LATS1 gene in mammalian cells using the CMV promoter

Depositor

Duojia Pan

Article

Wang et al Cell. 2022 Nov 10;185(23):4376-4393.e18.

Insert

LATS1 (LATS1 Human)

Use

Lentiviral

Tags

V5-RFP

Expression

Mammalian

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pK170.AAV-TRE-Cre-WPRE (Supernova)

Plasmid

#85040

Purpose

For AAV-TRE-Cre based Supernova, pK170 should be used with pK168 etc.

Depositor

Takuji Iwasato

Article

Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

Insert

nlsCre-WPRE

Use

AAV

Expression

Mammalian

Available Since

Nov. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pBABE_hygro_mRFP1_NRF2

Plasmid

#136579

Purpose

mammalian expression of mRFP1-tagged human NRF2

Depositor

Kevin Janes

Article

Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

Insert

NRF2 (NFE2L2 Human)

Use

Retroviral

Tags

N-mRFP1-FLAG

Expression

Mammalian

Available Since

July 14, 2020

Availability

Academic Institutions and Nonprofits only

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pACYCT2

Plasmid

#45799

Depositor

Luc Ponchon

Article

Ponchon et al Nucleic Acids Res. 2013 Aug;41(15):e150.

Type

Empty backbone

Expression

Bacterial

Available Since

Sept. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pJEP314-pAAV-U6SaCas9gRNA(SapI)-CMV-SaCas9-DIO-pA

Plasmid

#113691

Purpose

U6 driven SaCas9 gRNA expression cassette followed by a CMV driven inverted SaCas9. SaCas9 is floxed to render the system cre-dependent.

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Insert

SaCas9

Use

AAV, CRISPR, and Cre/Lox

Tags

NLS

Promoter

Cytomegalo Virus(CMV)

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pHR-UCOE-SFFV-Zim3-dCas9-BFP

Plasmid

#188776

Purpose

dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

Depositor

Marco Jost, Jonathan Weissman

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

Zim3-dCas9-mTagBFP2

Use

Lentiviral

Tags

HA-2xNLS-mTagBFP2 and Zim3 KRAB-NLS fusion

Promoter

SFFV

Available Since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pBSTNAV

Plasmid

#45801

Depositor

Luc Ponchon

Article

Ponchon et al Nucleic Acids Res. 2013 Aug;41(15):e150.

Type

Empty backbone

Expression

Bacterial

Available Since

Sept. 5, 2013

Availability

Academic Institutions and Nonprofits only

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psfaMs-Tn5

Plasmid

#183637

Purpose

Expresses anti-mouse nanobody fused with Tn5

Depositor

Goncalo Castelo-Branco

Article

Bartosovic et al Nat Biotechnol. 2023 Jun;41(6):794-805.

Insert

anti mouse secondary nanobody fused to Tn5

Tags

anti-mouse nanobody

Expression

Bacterial

Available Since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-CD14

Plasmid

#13645

Depositor

Doug Golenbock

Insert

CD14 (CD14 Human)

Expression

Mammalian

Available Since

June 12, 2007

Availability

Academic Institutions and Nonprofits only

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p413 TEF NAPstar4.3

Plasmid

#232743

Purpose

Expresses NADPH/NADP+ biosensor NAPstar4.3 in S. cerevisiae.

Depositor

Bruce Morgan

Article

Scherschel et al Nat Commun. 2024 Dec 19;15(1):10704. doi

Insert

NAPstar4.3

Expression

Yeast

Available Since

April 4, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGGS-Stargazin-mEGFP-iLID

Plasmid

#178523

Purpose

Expresses Stargazin-mEGFP-iLID in mammalian cells

Depositor

Kazuhiro Aoki

Article

Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

Insert

Stargazin-mEGFP-iLID

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-2xFYVE

Plasmid

#140047

Purpose

Expresses GFP-tagged HRS 2xFYVE in mammalian cells

Depositor

Harald Stenmark

Article

Gillooly et al EMBO J. 2000 Sep 1;19(17):4577-88. doi:

Insert

tandem HRS FYVE domain (Hgs Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

two FYVE domains (amino acids 147-223), linked by…

Promoter

CMV

Available Since

May 3, 2020

Availability

Academic Institutions and Nonprofits only

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