159,557 results

1. EMTB-3XGFP

   Plasmid

   #26741

   Depositor

   William Bement

   Article

   Miller et al Nat Cell Biol. 2009 Jan . 11(1):71-7.

   Insert

   ensconsin (MAP7 Human)
   

   Use

   Probe for microtubules

   Tags

   3x EGFP and Myc

   Expression

   Mammalian

   Mutation

   Only contains the microtubule binding-domain of e…

   Promoter

   Available since

   Feb. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET30-HPF1-His-Sumo-Flag

   Plasmid

   #111577

   Purpose

   Expresses full length HPF1 in E. coli

   Depositor

   Thomas Muir

   Article

   Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

   Insert

   HPF1 (HPF1 Human)
   

   Use

   Tags

   6xHis-Sumo-FLAG

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. Tg(mpeg1:LifeAct-mRuby)

   Plasmid

   #166977

   Purpose

   labels F-actin in macrophages

   Depositor

   Anna Huttenlocher

   Article

   Barros-Becker et al J Cell Sci. 2017 Nov 15;130(22):3801-380

   Inserts

   mpeg1 (mpeg1.1 Zebrafish)
   LifeAct (actb1 Frog)
   

   Use

   Tags

   Lifeact-mRuby and mRuby

   Expression

   Mutation

   Promoter

   Available since

   Oct. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. 2xP53_RE::FLuc

   Plasmid

   #178316

   Purpose

   P53 Firefly luciferase reporter

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Methods Mol Biol. 2022;2524:409-432. doi

   Insert

   P53 FLuc Reporter
   

   Use

   Luciferase and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV.CMV.SV40.THBS1-HA.SV40(polyA)

   Plasmid

   #154850

   Purpose

   Expresses murine Thrombospondin-1 (THBS1) protein with HA-tag at C-terminus

   Depositor

   Kevin Park

   Article

   Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

   Insert

   Thrombospondin-1 (Thbs1 Mouse)
   

   Use

   AAV

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3puro-mGFP-FMR1 isoform1-sh1Resistant

   Plasmid

   #225462

   Purpose

   Express mEGFP-fusion protein of FMR1 isoform 1

   Depositor

   Christine Mayr

   Article

   Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

   Insert

   FMR1 isoform 1 (FMR1 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pet30b-7M SrtA

   Plasmid

   #51141

   Purpose

   expression plasmid for C-terminal his tagged, calcium independent S.aureus SrtA with enhanced catalytic activity

   Depositor

   Hidde Ploegh

   Insert

   S.aureus SrtA 7M
   

   Use

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   P94R, E105K, E108Q, D160N, D165A, K190E, K196T

   Promoter

   T7

   Available since

   Feb. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFB2A

   Plasmid

   #195283

   Purpose

   Bicistronic DTR and Hygromycin resistance for positive/negative selection

   Depositor

   Scott Lowe

   Article

   Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

   Insert

   DTR 2A Hygro (HBEGF Synthetic, Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   Jan. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBAD-I-SceI

   Plasmid

   #60960

   Purpose

   Plasmid that allows an arabinose-inducible expression of the I-SceI homing endonuclease - for the en passant (two-step Red-mediated) mutagenesis

   Depositor

   Nikolaus Osterrieder

   Article

   Tischer et al Biotechniques. 2006 Feb;40(2):191-7.

   Insert

   I-SceI restriction enzyme
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX330A-1x2

    Plasmid

    #58766

    Purpose

    Expresses Cas9 nuclease and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 nuclease
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRK172-6xHis-0N4R-P301S-tau-HiBiT

    Plasmid

    #187835

    Purpose

    Expression of 6xHis-tagged P301S tau of 0N4R isoform with a C-terminal HiBiT tag for bimolecular luminescence with LgBiT.

    Depositor

    William McEwan

    Article

    Tuck et al Cell Rep. 2022 May 3;39(5):110776. doi:

    Insert

    6XHis-0N4R-P301S-tau-HiBiT
    

    Use

    Tags

    6xHistidine and HiBiT

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pmirGLO-3'UTR-TNF

    Plasmid

    #153069

    Purpose

    Human TNF 3'UTR region cloned downstream of luciferase reporter gene

    Depositor

    Silvia Monticelli

    Article

    Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

    Insert

    3' UTR region of TNF gene (TNF Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    July 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-TLR2-YFP

    Plasmid

    #13016

    Depositor

    Doug Golenbock

    Insert

    Toll-Like Receptor 2 (TLR2 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHR-tdPP7-3xmCherry

    Plasmid

    #74926

    Purpose

    Expresses tdPP7-3xmCherry

    Depositor

    Marvin Tanenbaum

    Article

    Yan et al Cell. 2016 May 5;165(4):976-89. doi: 10.

    Insert

    PP7-3xmCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCap-SG5-Apr

    Plasmid

    #227579

    Purpose

    Capture plasmid with apramycin resistance and pSG5 replicon

    Depositor

    Chengzhang Fu

    Article

    Xie et al Science. 2024 Dec 13;386(6727):eabq7333.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV G-CEPIA1er

    Plasmid

    #58215

    Purpose

    Green fluorescent indicator for calcium imaging in the endoplasmic reticulum

    Depositor

    Masamitsu Iino

    Article

    Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

    Insert

    G-CEPIA1er
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    G-GECO1.1 E299D M304L D328N N345K F360W G369S E37…

    Promoter

    CMV

    Available since

    July 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDB179-TurboPETase

    Plasmid

    #218693

    Purpose

    Encodes codon optimized TurboPETase enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

    Insert

    TurboPETase
    

    Use

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti-PGK-Hygro-PIP-FUCCI

    Plasmid

    #138714

    Purpose

    Lentiviral expression of the cell cycle marker PIP-FUCCI

    Depositor

    Jean Cook

    Article

    Grant et al Cell Cycle. 2018;17(21-22):2496-2516. do

    Insert

    PIP-FUCCI
    

    Use

    Lentiviral

    Tags

    mCherry and mVenus

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK

    Available since

    May 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBJ FLAG-hPar1

    Plasmid

    #53226

    Purpose

    mammalian expression of human Par1 with N-terminal FLAG tag

    Depositor

    Shaun Coughlin

    Article

    Ishii et al J Biol Chem. 1993 May 5. 268(13):9780-6.

    Insert

    Par1 (F2R Human)
    

    Use

    Tags

    FLAG and bovine prolactin signal sequence (MDSKGS…

    Expression

    Mammalian

    Mutation

    Deletion of aa1-39 (native signal peptide)

    Promoter

    SV40

    Available since

    Aug. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMito-iRFP670

    Plasmid

    #45462

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

    Insert

    iRFP670
    

    Use

    Tags

    human cytochrome C oxidase subunit VIII

    Expression

    Mammalian

    Mutation

    T2A, S45T, V116I, D124E, M170L, K180M, D202L, I20…

    Promoter

    CMV

    Available since

    June 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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