12,469 results

1. pLCHKO_Ptbp_ex8_1

   Plasmid

   #155081

   Purpose

   Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases (CHyMErA system)

   Depositor

   Benjamin Blencowe, Jason Moffat

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLCHKO_Ptbp_ex8_4

   Plasmid

   #155084

   Purpose

   Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases (CHyMErA system)

   Depositor

   Benjamin Blencowe, Jason Moffat

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLCHKO_Ptbp_ex8_2

   Plasmid

   #155082

   Purpose

   Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases (CHyMErA system)

   Depositor

   Benjamin Blencowe, Jason Moffat

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRDAX-537 C-Tag Venus210[2] L20 Itgb1 mCherry

   Plasmid

   #130016

   Purpose

   PPI (BiFC/TriFC) assay vector

   Depositor

   Gary Lewin

   Insert

   Itgb1 (Itgb1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pKLV-gRNA-Bcan-Ntrk1_4

   Plasmid

   #136413

   Purpose

   Lentiviral expression of gRNAs targeting intron 13 of murine Bcan and intron 10 of murine Ntrk1. Also constitutively expresses Puromycin linked to TagBFP.

   Depositor

   Massimo Squatrito

   Article

   Oldrini et al Nat Commun. 2018 Apr 13;9(1):1466. doi:

   Insert

   U6_sgRNA(Bcan)_U6_sgRNA(Ntrk1)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pKLV-gRNA-Myb-Qk_1

   Plasmid

   #136415

   Purpose

   Lentiviral expression of gRNAs targeting intron 4 of murine Qk and intron 9 of murine Myb. Also constitutively expresses Puromycin linked to TagBFP.

   Depositor

   Massimo Squatrito

   Article

   Oldrini et al Nat Commun. 2018 Apr 13;9(1):1466. doi:

   Insert

   U6_sgRNA(Myb)_U6_sgRNA(Qk)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. EGFP-Mm_Plk5(300-595) PBD domain

   Plasmid

   #136316

   Purpose

   Mammalian expression of mouse Plk5-D300 (PBD domain 300-595) with N-terminal EGFP

   Depositor

   Guillermo de Cárcer, Marcos Malumbres

   Article

   de Carcer et al Mol Cell Biol. 2011 Mar;31(6):1225-39. d

   Insert

   PLK5 (Plk5 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   fragment 300-595

   Promoter

   Available since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. EGFP-Mm_Plk5(D168A)

   Plasmid

   #136318

   Purpose

   Mammalian expression of mouse Plk5-KD (D168A) with N-terminal EGFP

   Depositor

   Guillermo de Cárcer, Marcos Malumbres

   Article

   de Carcer et al Mol Cell Biol. 2011 Mar;31(6):1225-39. d

   Insert

   PLK5 (Plk5 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D168A

   Promoter

   Available since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pENTR-Mm_MyoGEF(WT)

   Plasmid

   #136327

   Purpose

   Mouse MyoGEF-WT in Gateway pENTR

   Depositor

   Guillermo de Cárcer, Marcos Malumbres

   Article

   de Carcer et al Nat Med. 2017 Aug;23(8):964-974. doi: 10

   Insert

   PLEKHG6 (Plekhg6 Mouse)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pENTR-Mm_MyoGEF(T575A)

    Plasmid

    #136328

    Purpose

    Mouse MyoGEF-T575A mutant in Gateway pENTR

    Depositor

    Guillermo de Cárcer, Marcos Malumbres

    Article

    de Carcer et al Nat Med. 2017 Aug;23(8):964-974. doi: 10

    Insert

    PLEKHG6 (Plekhg6 Mouse)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    T575A

    Promoter

    Available since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMAL-C2X dAmp/Kana+-2MT

    Plasmid

    #154353

    Purpose

    Bacterial expression of 2MT

    Depositor

    Wanzhong He

    Article

    Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

    Insert

    2MT
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCas9n-Nanog-R

    Plasmid

    #122303

    Purpose

    Expresses sgRNA targeting mouse Nanog and Cas9 nickase in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

    Insert

    sgRNA for mouse Nanog (Nanog Synthetic, Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Antares-SEPLuc C1A4E

    Plasmid

    #141088

    Purpose

    precursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosis; utilizes weaker mutant of IRES and weaker precursor of Nanoluc

    Depositor

    Jeak Ling Ding

    Article

    Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

    Insert

    Antares-T2A-puromycin-IRESv24-SEP-C1A4E
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Please see depositor comments

    Promoter

    CMV

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Anti-ZNF423 [N328B/37R]

    Plasmid

    #140077

    Purpose

    Mammalian Expression Plasmid of anti-ZNF423 (Human). Derived from hybridoma N328B/37.

    Depositor

    James Trimmer

    Article

    Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-ZNF423 (Human) recombinant mouse monoclonal antibody (ZNF423 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    dual CMV

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Anti-Mad3 [N129A/6R]

    Plasmid

    #140075

    Purpose

    Mammalian Expression Plasmid of anti-Mad3 (Human). Derived from hybridoma N129A/6.

    Depositor

    James Trimmer

    Article

    Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-Mad3 (Human) recombinant mouse monoclonal antibody (MXD3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    dual CMV

    Available since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Anti-Kir2.2 K+ channel [N124B/38R]

    Plasmid

    #140074

    Purpose

    Mammalian Expression Plasmid of anti-Kir2.2 K+ channel (Rat). Derived from hybridoma N124B/38.

    Depositor

    James Trimmer

    Article

    Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-Kir2.2 K+ channel (Rat) recombinant mouse monoclonal antibody (Kcnj12 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    dual CMV

    Available since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. SEPLuc-E2A-cyOFP1

    Plasmid

    #141084

    Purpose

    precursor of the bioluminescent reporter pHLuc, for detecting extracellular pH changes in tumor acidosis

    Depositor

    Jeak Ling Ding

    Article

    Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

    Insert

    SEPLuc-E2A-cyOFP1
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Anti-Kv1.5 K+ channel [K7/45R]

    Plasmid

    #140069

    Purpose

    Mammalian Expression Plasmid of anti-Kv1.5 K+ channel (Rat). Derived from hybridoma K7/45.

    Depositor

    James Trimmer

    Article

    Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-Kv1.5 K+ channel (Rat) recombinant mouse monoclonal antibody (Kcna5 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    dual CMV

    Available since

    June 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Anti-KCNT1/Slo2.2/Slack K+ channel [N3/26R]

    Plasmid

    #140064

    Purpose

    Mammalian Expression Plasmid of anti-KCNT1/Slo2.2/Slack K+ channel (Rat). Derived from hybridoma N3/26.

    Depositor

    James Trimmer

    Article

    Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-KCNT1/Slo2.2/Slack K+ channel (Rat) recombinant mouse monoclonal antibody (Kcnt1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    dual CMV

    Available since

    June 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. sg1

    Plasmid

    #113966

    Purpose

    Single short guide RNA targeting GTATAGCATACATTATACG

    Depositor

    Jordan Green

    Article

    Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

    Insert

    sg1
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. sg2

    Plasmid

    #113967

    Purpose

    Single short guide RNA targeting TACCACATTTGTAGAGGTT

    Depositor

    Jordan Green

    Article

    Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

    Insert

    sg2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. sg3

    Plasmid

    #113968

    Purpose

    Single short guide RNA targeting CAATGTATCTTATCATGTC

    Depositor

    Jordan Green

    Article

    Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

    Insert

    sg3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. sg2+sg3

    Plasmid

    #113970

    Purpose

    Double short guide RNA targeting TACCACATTTGTAGAGGTT & CAATGTATCTTATCATGTC

    Depositor

    Jordan Green

    Article

    Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

    Insert

    sg2+sg3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMBP-parallel1-GK5_v2 (D30N+D280N+D443N)

    Plasmid

    #134291

    Purpose

    Expresses GK5 (isoform 2)-D30N/D280N/D443N mutant in bacteria

    Depositor

    Bruce Beutler

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

    Insert

    Gk5 (Gk5 Mouse)
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    changed Aspartic Acids 30, 280 and 443 to Asparag…

    Promoter

    T7

    Available since

    May 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pBOB-N-3HA-GK_v2

    Plasmid

    #134277

    Purpose

    Lentivector encoding 3XHA-tagged GK (isoform 2)

    Depositor

    Bruce Beutler

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

    Insert

    Gk (Gk Mouse)
    

    Use

    Lentiviral

    Tags

    3X HA

    Expression

    Mammalian

    Mutation

    553 amino acids, isoform 2

    Promoter

    CMV

    Available since

    May 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pBOB-N-Flag-GK (281-473)

    Plasmid

    #134273

    Purpose

    Lentivector encoding Flag-tagged GK truncation (amino acids 281-473)

    Depositor

    Bruce Beutler

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

    Insert

    Gk (Gk Mouse)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    mouse Gk amino acids 281-473

    Promoter

    CMV

    Available since

    May 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pBOB-N-Flag-GK (12-272)

    Plasmid

    #134272

    Purpose

    Lentivector encoding Flag-tagged GK truncation (amino acids 12-272)

    Depositor

    Bruce Beutler

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

    Insert

    Gk (Gk Mouse)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    mouse Gk amino acids 12-272

    Promoter

    CMV

    Available since

    May 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBOB-N-Flag-GK_v1

    Plasmid

    #134270

    Purpose

    Lentivector encoding Flag-tagged GK (isoform 1)

    Depositor

    Bruce Beutler

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

    Insert

    Gk (Gk Mouse)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    mouse Gk isoform 1; 524 amino acids

    Promoter

    CMV

    Available since

    May 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pRDAX-VALENTIA-005 Lyn-Neptune//BsaI

    Plasmid

    #130186

    Purpose

    EMMA Kit # 1000000119 extension

    Depositor

    Gary Lewin

    Insert

    Lyn-Neptune
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pRDAX-VALENTIA-010 LifeAct-Neptune//BsaI

    Plasmid

    #130191

    Purpose

    EMMA Kit # 1000000119 extension

    Depositor

    Gary Lewin

    Insert

    LifeAct-Neptune
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pRDAX-VALENTIA-015 Vimentin-Neptune//BsaI

    Plasmid

    #130196

    Purpose

    EMMA Kit # 1000000119 extension

    Depositor

    Gary Lewin

    Insert

    Vimentin-Neptune
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pRDAX-VALENTIA-016 NLSNoLS-Sirius//BsaI

    Plasmid

    #130197

    Purpose

    EMMA Kit # 1000000119 extension

    Depositor

    Gary Lewin

    Insert

    NLSNoLS-Sirius
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pRDAX-VALENTIA-017 NLSNoLS-SuperNova//BsaI

    Plasmid

    #130198

    Purpose

    EMMA Kit # 1000000119 extension

    Depositor

    Gary Lewin

    Insert

    NLSNoLS-SuperNova
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pRDAX-VALENTIA-033 NLSNoLS-Eos//BsaI

    Plasmid

    #130214

    Purpose

    EMMA Kit # 1000000119 extension

    Depositor

    Gary Lewin

    Insert

    NLSNoLS-Eos
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pRDAX-615 C-Tag Venus210[1] L20 Stat3(R609Q) EBPF2

    Plasmid

    #130090

    Purpose

    PPI (BiFC/TriFC) assay vector

    Depositor

    Gary Lewin

    Insert

    Stat3(R609Q)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R609Q

    Promoter

    Available since

    May 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pRDAX-614 N-Tag Venus210[1] L10 Stat3(R609Q) EBPF2

    Plasmid

    #130089

    Purpose

    PPI (BiFC/TriFC) assay vector

    Depositor

    Gary Lewin

    Insert

    Stat3(R609Q)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R609Q

    Promoter

    Available since

    May 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pRDAX-611 C-Tag Venus210[2] L32 Stat3 mCherry

    Plasmid

    #130086

    Purpose

    PPI (BiFC/TriFC) assay vector

    Depositor

    Gary Lewin

    Insert

    Stat3 (Stat3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pRDAX-603 C-Tag Venus210[1] L20 Stat3 EBPF2

    Plasmid

    #130078

    Purpose

    PPI (BiFC/TriFC) assay vector

    Depositor

    Gary Lewin

    Insert

    Stat3 (Stat3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pRDAX-527 C-Tag GFP[1] L32 Tln1(rod) mCherry

    Plasmid

    #130054

    Purpose

    PPI (BiFC/TriFC) assay vector

    Depositor

    Gary Lewin

    Insert

    Tln1 rod
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pRDAX-526 N-Tag GFP[1] L20 Tln1(rod) mCherry

    Plasmid

    #130053

    Purpose

    PPI (BiFC/TriFC) assay vector

    Depositor

    Gary Lewin

    Insert

    Tln1 rod
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pBOB-C-Flag-GK5-toku (1-174)

    Plasmid

    #134261

    Purpose

    Lentivector encoding Flag-tagged toku form of GK5 (amino acids 1-174)

    Depositor

    Bruce Beutler

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

    Insert

    Gk5 (Gk5 Mouse)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    mouse Gk5 amino acids 1-174

    Promoter

    CMV

    Available since

    May 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. Antares-SEPLuc IRESv24

    Plasmid

    #141087

    Purpose

    precursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosis; utilizes weaker mutant of IRES

    Depositor

    Jeak Ling Ding

    Article

    Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

    Insert

    Antares-T2A-puromycin-IRESv24-SEPLuc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. mPLD2-PX (62-192)

    Plasmid

    #119128

    Purpose

    Bacterial expression of human phox homology (PX) domain, mPLD2-PX (62-192)

    Depositor

    Brett Collins

    Article

    Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

    Insert

    mPLD2-PX (62-192)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    April 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. Y365A or A365Y inducible rescue

    Plasmid

    #131323

    Purpose

    gRNA to generate i-WT-r or i-MT-r systems

    Depositor

    Danny Reinberg

    Article

    Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

    Insert

    Y365A or A365Y inducible rescue
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pSECC-sgSIK1-B

    Plasmid

    #138666

    Purpose

    Expresses a mouse SIK1-targeting sgRNA, Cas9, and Cre-recombinase

    Depositor

    Reuben Shaw

    Article

    Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

    Insert

    sgSIK1 mouse (Sik1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    March 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pMBP-parallel1-GK5_v2 (D443N)

    Plasmid

    #134293

    Purpose

    Expresses GK5 (isoform 2)-D443N mutant in bacteria

    Depositor

    Bruce Beutler

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

    Insert

    Gk5 (Gk5 Mouse)
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    changed Aspartic Acid 443 to Asparagine

    Promoter

    T7

    Available since

    March 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pMBP-parallel1-GK5_v2 (D30N+D280N)

    Plasmid

    #134292

    Purpose

    Expresses GK5 (isoform 2)-D30N/D280N mutant in bacteria

    Depositor

    Bruce Beutler

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

    Insert

    Gk5 (Gk5 Mouse)
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    changed Aspartic Acids 30 and 280 to Asparagine

    Promoter

    T7

    Available since

    March 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pMBP-parallel1-GK5_v2 (D280N)

    Plasmid

    #134290

    Purpose

    Expresses GK5 (isoform 2)-D280N mutant in bacteria

    Depositor

    Bruce Beutler

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

    Insert

    Gk5 (Gk5 Mouse)
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    changed Aspartic Acid 280 to Asparagine

    Promoter

    T7

    Available since

    March 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pMBP-parallel1-GK5_v2 (D30N)

    Plasmid

    #134289

    Purpose

    Expresses GK5 (isoform 2)-D30N mutant in bacteria

    Depositor

    Bruce Beutler

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

    Insert

    Gk5 (Gk5 Mouse)
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    changed Aspartic Acid 30 to Asparagine

    Promoter

    T7

    Available since

    March 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pcDNA6-C-Flag-GK5_v1

    Plasmid

    #134281

    Purpose

    Expresses Flag-tagged GK5 (isoform 1) in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

    Insert

    Gk5 (Gk5 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    mouse Gk5 isoform 1; 516 amino acids

    Promoter

    CMV

    Available since

    March 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge