20,093 results

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pHREAC

Plasmid

#134908

Purpose

Overexpression of recombinant proteins in plants thanks to very efficient translational efficiency. Empty vector, clone ORF using Bsa1 sites. Download fully annotated map under Resource Information.

Depositor

George Lomonossoff

Article

Peyret et al Plant Methods. 2019 Sep 18;15:108. doi:

Type

Empty backbone

Use

Synthetic Biology

Expression

Plant

Promoter

double 35S

Available Since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pLGB13

Plasmid

#126618

Purpose

Suicide vector for allelic replacement in Bacteroides, erythromycin selection and aTC-inducible ss-Bfe1 counterselection

Depositor

Laurie Comstock

Article

Garcia-Bayona et al mBio. 2019 Aug 13;10(4). pii: mBio.01762

Type

Empty backbone

Use

Allellic exchange in bacteria

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pHR-EF1Alpha-puro-T2A-Tet-on 3G-TRE3G-Ascl1

Plasmid

#118593

Purpose

Expresses a puromycin resistance cassette with Tet-on 3G from the EF1Alpha promoter and Ascl1 from the TRE3G promoter

Depositor

Stanley Qi

Article

Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

Insert

Puro-T2A-Tet-on 3G/Ascl1 (Ascl1 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1Alpha/TRE3G

Available Since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pCasKP-apr

Plasmid

#117231

Purpose

Bacterial expression of Cas9 nuclease and lambda-Red system in Klebsiella Pneumoniae

Depositor

Quanjiang Ji

Article

Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

Insert

Cas9

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

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anxA2-GFP

Plasmid

#107196

Purpose

Expresses human annexin A2-GFP fusion protein for live cell imaging

Depositor

Volker Gerke, Ursula Rescher

Article

Rescher et al J Cell Sci. 2000 Nov;113 ( Pt 22):3931-8

Insert

annexin A2 (ANXA2 Human)

Tags

Green Fluorescent Protein

Expression

Mammalian

Mutation

Ala residue 65 in human sequence replaced by Glu …

Promoter

CMV

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-eGFP-hcGAS

Plasmid

#108674

Purpose

For production of retrovirus and transduction of mammalian cells, allowing expression of N-terminally eGFP-tagged human cGAS

Depositor

Andrew Jackson, Martin Reijns

Article

Mackenzie et al Nature. 2017 Aug 24;548(7668):461-465. d

Insert

eGFP-cGAS (CGAS Human)

Use

Retroviral

Promoter

LTR

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pHR-mCh-Cry2olig

Plasmid

#101222

Purpose

Cry2olig(E490G)

Depositor

Clifford Brangwynne

Article

Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

Insert

Cry2olig

Use

Lentiviral

Expression

Mammalian

Mutation

E490G

Promoter

SFFV

Available Since

Feb. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pEB2-mScarlet-I

Plasmid

#104007

Purpose

Plasmid encoding mScarlet-I

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mScarlet-I

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to DsRed (MSKGE AVIKEF… N6A, R…

Promoter

proC

Available Since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pEN435 - pCAGGS-TagBFP-hGeminin-2A-mCherry-hCdt1-rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt Tigre targeting

Plasmid

#92139

Purpose

Targeting vector to introduce a Fucci cell cyle reporter cassette mCherry(G1)/BFP(S/G2) at the mouse TIGRE acceptor locus using puromycin selection

Depositor

Benoit Bruneau

Article

Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

Insert

chromatin licensing and DNA replication factor 1 (GMNN, CDT1 Human)

Use

Mouse Targeting

Expression

Mammalian

Available Since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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pLV-Ftractin-mCherry

Plasmid

#85131

Purpose

lentiviral expression of an F-actin live-cell marker (mCherry)

Depositor

Tobias Meyer

Article

Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

Insert

Ftractin-mCherry (amino acids 9-52 of rat ITPKA, C-temrinally tagged with mCherry)

Use

Lentiviral

Tags

mCherry

Promoter

EF1a

Available Since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pmScarlet_H2A_C1

Plasmid

#85051

Purpose

In vivo visualization of the nucleus (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

H2A (H2AC16 Human)

Tags

mScarlet

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pNCS-CyOFP

Plasmid

#74278

Purpose

Constitutive expression of CyOFP in bacteria

Depositor

Michael Lin

Article

Chu et al Nat Biotechnol. 2016 May 30. doi: 10.103

Insert

CyOFP

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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pTriEx4-Cdc42-2G

Plasmid

#68814

Purpose

FRET-based biosensor reporting on endogenous Cdc42 activation (for expression in E. coli, insect and vertebrate cells)

Depositor

Olivier Pertz

Article

Martin et al Sci Rep. 2016 Feb 25;6:21901. doi: 10.10

Insert

Cdc42 activation reporter

Tags

His and Myc

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pORI28

Plasmid

#71595

Purpose

EC1000 containing pORI28. Used as the source of plasmid pORI28. With pTRK669, part of a system for chromosomal integration in gram positive bacteria.

Depositor

Todd Klaenhammer, Jan Kok

Article

Leenhouts et al Mol Gen Genet. 1996 Nov 27;253(1-2):217-

Type

Empty backbone

Use

Integration vector for bacteria

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/DEST/hTfR-HA

Plasmid

#69610

Purpose

human transferrin receptor with an HA tag fused at the C-terminus

Depositor

Robin Shaw

Article

Smyth et al Circ Res. 2012 Mar 30;110(7):978-89. Epu

Insert

human transferrin receptor (TFRC Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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MDM2p-Mdm2-YFP

Plasmid

#53962

Purpose

Expresses YFP tagged Mdm2 from MDM2 promoter

Depositor

Uri Alon, Galit Lahav

Article

Lahav et al Nat Genet. 2004 Feb;36(2):147-50. Epub 2

Insert

MDM2 (MDM2 Human)

Tags

EYFP

Expression

Mammalian

Promoter

MDM2

Available Since

Aug. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pLenti.CAG.H2B-Dendra2.W

Plasmid

#51005

Purpose

Lentivector that expresses H2B-Dendra2 from internal CAG enhancer-promoter

Depositor

Rusty Lansford

Insert

H2B-Dendra2

Use

Lentiviral

Tags

histone2B

Expression

Mammalian

Promoter

CAG

Available Since

June 2, 2014

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yoEGFP-Kan

Plasmid

#44900

Purpose

Yeast tagging vector to create gene fusion with yeast optimized EGFP with Kan selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoEGFP

Expression

Yeast

Available Since

May 9, 2013

Availability

Academic Institutions and Nonprofits only

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3XFlagNICD3

Plasmid

#20185

Depositor

Raphael Kopan

Article

Ong et al J Biol Chem. 2006 Feb 24. 281(8):5106-19

Insert

Notch 3 Intracellular Domain (Notch3 Mouse)

Tags

3X Flag

Expression

Mammalian

Mutation

Contains murine Notch 3 Intracellular Domain (Met…

Promoter

CMV

Available Since

Dec. 13, 2012

Availability

Academic Institutions and Nonprofits only

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pCI-neo.mCherry-NEDD4

Plasmid

#38316

Depositor

Quan Lu

Article

Nabhan et al EMBO Rep. 2010 Aug;11(8):605-11. Epub 20

Insert

Nedd4 (NEDD4 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

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