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19,776 results

Showing: 4501 - 4520 of 19776 results

  9. pcDNA-dCas9-p300 Core (D1399Y)

    Plasmid
    #61358
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation D1399Y) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, S. pyogenes, Human)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; D1399Y mutation i…
    Promoter
    CMV
    Available Since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61358-plasmid-map-sequence-id-99789

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  12. pEvol-MjaYRS

    Plasmid
    #153557
    Purpose
    Amber suppression for incorporating 3-aminotyrosine to proteins in E. coli
    Depositor
    Huiwang Ai
    Article
    Zhang et al Nat Chem Biol. 2020 Sep 14. pii: 10.1038
    Inserts
    MJaYRS (first copy)
    MJaYRS (second copy)
    amber suppression tRNA under proK promoter
    Expression
    Bacterial
    Promoter
    glnS and pBAD
    Available Since
    July 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    153557-plasmid-map-sequence-id-306290

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  14. ER-GA

    Plasmid
    #209871
    Purpose
    Dimerization dependent fluorescent protein GA anchored to cytosolic face of the endoplasmic reticulum membrane with N-terminal targeting sequence of rabbit CYP2C1
    Depositor
    Sarah Cohen
    Article
    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25
    Insert
    ddGFP A
    Tags
    Targeting domain of CYP2C1 MDPVVVLGLCLSCLLLLSLWKQ…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    209871-plasmid-map-sequence-id-415464

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  15. PX551

    Plasmid
    #60957
    Purpose
    pAAV-pMecp2-SpCas9-spA (AAV-SpCas9). AAV plasmid expressing Cas9 in neurons under control of truncated mecp2 promoter.
    Depositor
    Feng Zhang
    Article
    Swiech et al Nat Biotechnol. 2014 Oct 19. doi: 10.103
    Insert
    SpCas9
    Use
    AAV and CRISPR
    Tags
    HA
    Expression
    Mammalian
    Promoter
    pMecp2
    Available Since
    Nov. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    60957-plasmid-map-sequence-id-181490

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  18. p5E-mpeg1.1

    Plasmid
    #75023
    Purpose
    Can be used to drive expression in zebrafish microglia.
    Depositor
    Nicholas Cole
    Article
    Don et al Zebrafish. 2016 Sep 15.
    Insert
    macrophage expressed 1, tandem duplicate 1 (mpeg1.1 Zebrafish)
    Use
    5' entry vector for multisite gateway three …
    Promoter
    mpeg1.1
    Available Since
    Oct. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    75023-plasmid-map-sequence-id-141914

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Showing: 4501 - 4520 of 19776 results