We narrowed to 17,688 results for: She

1. pCR4-MBII52-probe

   Plasmid

   #67647

   Purpose

   antisense RNA probe for RNase protection or nothern

   Depositor

   Stefan Stamm

   Article

   Falaleeva et al Gene. 2015 Jul 26. pii: S0378-1119(15)00

   Insert

   MBII52 snoRNA (Snord115 Mouse)
   

   Use

   For in vitro transcription

   Available Since

   Aug. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pSA107

   Plasmid

   #59785

   Purpose

   drives ZIF-1 protein in C. elegans ubiquitously

   Depositor

   Jeremy Nance

   Article

   Armenti et al Development. 2014 Dec;141(23):4640-7. do

   Inserts

   cdc-42 promoter (NEWENTRY Nematode)
   zif-1 coding sequence (zif-1 )
   unc-54 3' UTR
   

   Expression

   Bacterial

   Available Since

   Oct. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOPTN1-55-EGFP

   Plasmid

   #68838

   Purpose

   deleted OPTN was inserted into pEGFP-N1 backbone

   Depositor

   Beatrice Yue

   Article

   Turturro et al Springerplus. 2014 Feb 19;3:99. doi: 10.

   Insert

   optineurin (OPTN Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   aa 1-55 only

   Promoter

   CMV

   Available Since

   Nov. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

4. AKT3

   Plasmid

   #39038

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   AKT3 (AKT3 Human)
   

   Tags

   His Tev

   Expression

   Bacterial

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

5. MS2_SC2_S_Omicron

   Plasmid

   #180229

   Purpose

   Generation of MS2 Virus-Like Particles packaged with the sequence for the WHO Omicron SARS-CoV-2 S gene (Amino Acid 319-869)

   Depositor

   Paul Freemont

   Inserts

   Maturation Protein
   Coat Protein Dimer
   S gene (S SARS-CoV-2)
   

   Expression

   Bacterial

   Mutation

   Omicron (Amino Acid 319-869)

   Promoter

   T7

   Available Since

   Jan. 28, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. MS2_SC2_S_WT

   Plasmid

   #175044

   Purpose

   Generation of MS2 Virus-Like Particles packaged with the sequence for the WT SARS-CoV-2 S gene (Amino Acid 319-869)

   Depositor

   Paul Freemont

   Inserts

   Maturation Protein
   Coat Protein Dimer
   S gene (S SARS-CoV-2)
   

   Expression

   Bacterial

   Mutation

   WT (Amino Acid 319-869)

   Promoter

   T7

   Available Since

   Oct. 18, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pDM_noro_S6_lacZA

   Plasmid

   #118817

   Purpose

   T7 RNAP-driven expression of norovirus sense orientation toehold switches (S6) with a lacZ alpha subunit reporter.

   Depositor

   Alexander Green

   Article

   Ma et al Synth Biol (Oxf). 2018;3(1):ysy018. doi:

   Insert

   Norovirus toehold switch S6
   

   Expression

   Bacterial

   Available Since

   Jan. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSP64T-dnWnt-11

   Plasmid

   #16857

   Depositor

   Jim Smith

   Article

   Tada et al Development. 2000 May . 127(10):2227-38.

   Insert

   dnWnt-11 (wnt11b.L Frog)
   

   Use

   Xenopus expression

   Mutation

   amino acids 1-282. Dominant Negative.

   Available Since

   May 27, 2008

   Availability

   Academic Institutions and Nonprofits only

9. human KANK1 Ankyrin Repeats pET151

   Plasmid

   #164053

   Purpose

   Expresses human KANK1 Ank domain in bacterial cells

   Depositor

   Ben Goult

   Insert

   Kidney Ankyrin Repeat-Containing Protein 1 (KANK1 Human)
   

   Tags

   His-tag, TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1(+)MBII85

    Plasmid

    #67643

    Purpose

    MBII85 snoRNA expression cassett, contains snoRNA in natural intron surrounded by natural exons.

    Depositor

    Stefan Stamm

    Article

    Falaleeva et al Gene. 2015 Jul 26. pii: S0378-1119(15)00

    Insert

    MBII85 snoRNA
    

    Expression

    Mammalian

    Available Since

    Aug. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. human EB1-GFP mutagenized S16 R17 H18 D19 W23 N25 all converted to A (JB139)

    Plasmid

    #39300

    Depositor

    Tim Mitchison, Jennifer Tirnauer

    Insert

    EB1 (MAPRE1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S16A, R17A, H18A, D19A, W23A and N25A

    Promoter

    CMV

    Available Since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pCDNA3-FLAG-SENP3-C526A

    Plasmid

    #115522

    Purpose

    Mammalian expression construct

    Depositor

    Martine Roussel

    Article

    Kuo et al Cell Cycle. 2008 Nov 1;7(21):3378-87. do

    Insert

    SENP3-C526A (Senp3 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    C526A

    Available Since

    Sept. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKA-144

    Plasmid

    #79839

    Purpose

    Expresses RpBphP1-mCherry-Intersectin1 DHPH domain under CMVd1

    Depositor

    Vladislav Verkhusha

    Article

    Kaberniuk et al Nat Methods. 2016 May 9. doi: 10.1038/nm

    Insert

    RpBphP1-mCherry-Intersectin1 DHPH domain
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMVd1

    Available Since

    Aug. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLJM1-FLAG-Sestrin2-E451A

    Plasmid

    #73677

    Purpose

    overexpression

    Depositor

    David Sabatini

    Article

    Wolfson et al Science. 2016 Jan 1;351(6268):43-8. doi:

    Insert

    Sesn2 (SESN2 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    E451A changed Glutamate 451 to Alanine

    Promoter

    CMV

    Available Since

    March 30, 2016

    Availability

    Academic Institutions and Nonprofits only

15. CYP7A1 (3SN5)

    Plasmid

    #32874

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    CYP7A1 (CYP7A1 Human)
    

    Expression

    Bacterial

    Mutation

    amino acids 25-503 only

    Available Since

    Jan. 13, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

16. pTorPE-Y-GECO2f

    Plasmid

    #105065

    Purpose

    A yellow fluorescent Ca2+ indicator variant with low binding affinity and fast kinetics for expression in bacterial

    Depositor

    Robert Campbell

    Article

    Zhao et al Sci Rep. 2018 Aug 6;8(1):11758. doi: 10.

    Insert

    Y-GECO2f
    

    Tags

    6x His tag

    Expression

    Bacterial

    Promoter

    pBAD

    Available Since

    April 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV-SYN-PIK3CD

    Plasmid

    #203730

    Purpose

    AAV vector plasmid expressing human phosphoinositide 3-kinase catalytic subunit delta (PIK3CD) under the human synapsin (SYN) promoter

    Depositor

    Richard Eva, Bart Nieuwenhuis

    Insert

    PIK3CD (PIK3CD Human)
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Human synapsin promoter

    Available Since

    July 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRK5-FLAG-Sestrin2-S190W

    Plasmid

    #73670

    Purpose

    overexpression

    Depositor

    David Sabatini

    Article

    Wolfson et al Science. 2016 Jan 1;351(6268):43-8. doi:

    Insert

    Sesn2 (SESN2 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S190W changed serine 190 to tryptophan

    Promoter

    CMV

    Available Since

    March 30, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA6-rbNHERF1

    Plasmid

    #32942

    Depositor

    Edward Weinman

    Article

    Weinman et al Am J Physiol Renal Physiol. 2009 Dec;297

    Insert

    SLC9A3R1 (SLC9A3R1 rabbit)
    

    Tags

    T7 tag, his, and partial FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUASp YFP Rac1 CA

    Plasmid

    #52235

    Purpose

    Drosophila expression of YFP-Rac1 constitutively active mutant

    Depositor

    Matthew Scott

    Insert

    Rac1 (Rac1 Fly)
    

    Use

    Drosophila

    Tags

    YFP

    Expression

    Insect

    Mutation

    Q61L; CA mutation

    Promoter

    GAL4

    Available Since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

21. WDR5

    Plasmid

    #36900

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    WDR5 (WDR5 Human)
    

    Tags

    His tag with TEV cleavage

    Expression

    Bacterial

    Mutation

    mutation version of plasmid #60121 3UR4

    Available Since

    Aug. 8, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

22. pAIO-AsCpf1

    Plasmid

    #137846

    Purpose

    AsCpf1 and gRNA expression plasmid for P. falciparum; no selection in parasites.

    Depositor

    Josh Beck

    Article

    Nessel et al Cell Microbiol. 2020 Jan 28:e13168. doi:

    Insert

    AsCpf1-NLS-FLAG
    

    Use

    CRISPR

    Available Since

    April 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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23. pT7_circRAJ31_C873U

    Plasmid

    #69932

    Purpose

    Expresses RAJ31 riboregulator encased in a permuted intron exon ribozyme, which circularises the riboregulator. This mutant is deficient in circularisation. Under control of a T7 promoter.

    Depositor

    Alfonso Jaramillo

    Article

    Rostain et al Biodes Res. 2020 Sep 12;2020:1916789. do

    Insert

    pT7_circRAJ31_C873U
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Hs.KRAS4a Q61L

    Plasmid

    #83171

    Purpose

    Gateway ORF Entry clone of human KRAS4A [NM_033360.3] with stop codon (for native or N-terminal fusions), Q61L mutation

    Depositor

    Dominic Esposito

    Insert

    KRAS (KRAS Human)
    

    Use

    Gateway entry clone

    Mutation

    Q61L

    Available Since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pPNT6-eGFP

    Plasmid

    #71303

    Purpose

    This vector can be used for homologous recombination and allows to obtain recombinant neomycin resistant fluorescent cells that can loose fluorescence after Cre expression

    Depositor

    Marco Musso, Gian Luca Forni

    Type

    Empty backbone

    Use

    Cre/Lox

    Expression

    Mammalian

    Available Since

    March 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. LMNB2

    Plasmid

    #36896

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    LMNB2 (LMNB2 Human)
    

    Tags

    His tag with TEV cleavage

    Expression

    Bacterial

    Mutation

    contains Laminb2 Ig-like domain, amino acid resid…

    Available Since

    June 19, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

27. MAT2B

    Plasmid

    #39080

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    MAT2B (MAT2B Human)
    

    Tags

    Tev His Flag

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

28. Hs.KRAS4b A146T

    Plasmid

    #83150

    Purpose

    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), A146T mutation

    Depositor

    Dominic Esposito

    Insert

    KRAS (KRAS Human)
    

    Use

    Gateway entry clone

    Mutation

    A146T

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLPS-hAkt1-gfp

    Plasmid

    #49388

    Purpose

    mammalian expression of human Akt1 fused to EGFP

    Depositor

    Randall McKinnon

    Insert

    Akt1 (AKT1 Human)
    

    Use

    Cre/Lox; Acceptor vector

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    tga to cca at nt 1725 with respect to GenBank ID …

    Promoter

    CMV

    Available Since

    Nov. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

30. pCMV-T7-SpG-P2A-mScarlet (MNW006)

    Plasmid

    #174136

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpG(D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet

    Depositor

    Benjamin Kleinstiver

    Insert

    human codon optimized SpG with BPNLS-3xFLAG-P2A-mScarlet
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-mScarlet

    Expression

    Mammalian

    Mutation

    SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R

    Promoter

    CMV and T7

    Available Since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. Hs.KRAS4a Q61R

    Plasmid

    #83172

    Purpose

    Gateway ORF Entry clone of human KRAS4A [NM_033360.3] with stop codon (for native or N-terminal fusions), Q61R mutation

    Depositor

    Dominic Esposito

    Insert

    KRAS (KRAS Human)
    

    Use

    Gateway entry clone

    Mutation

    Q61R

    Available Since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. DHRS4

    Plasmid

    #39071

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    DHRS4 (DHRS4 Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

33. pTXB1-CRP/CAP

    Plasmid

    #51563

    Purpose

    Expression of E. coli CRP/CAP for protein purification (Intein-chiting binding domain fussion, New England Biolabs pTXB1 backbone

    Depositor

    Sankar Adhya, Francisco Malagon

    Insert

    CRP/CAP
    

    Tags

    Intein-Chiting Binding Domanin

    Expression

    Bacterial

    Available Since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. ASPH

    Plasmid

    #39094

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    ASPH (ASPH Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

35. NLuc CMV-Neo Flexi Vector pFC32K SARS-CoV2 5'Nsp1

    Plasmid

    #163319

    Purpose

    Encodes for first COVID protein - NSP1 fused to nano-luciferase

    Depositor

    Dawn Chandler

    Insert

    Nsp1 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Luciferase

    Tags

    NanoLuc

    Expression

    Mammalian

    Available Since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. Myc-gamma3 (in pCDNA3.1)

    Plasmid

    #118041

    Purpose

    GABAA receptor expression (gamma3 subunit) Myc-tag near N-terminus

    Depositor

    Angel de Blas

    Insert

    GABRG3 (Gabrg3 Rat)
    

    Tags

    cMyc epitope EQKLISEEDL inserted between fourth a…

    Expression

    Mammalian

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. Myc-gamma1 (in pCDNA3.1)

    Plasmid

    #118038

    Purpose

    GABAA receptor expression (gamma1 subunit) Myc-tag near N-terminus

    Depositor

    Angel de Blas

    Insert

    GABRG1 (Gabrg1 Rat)
    

    Tags

    cMyc epitope EQKLISEEDL inserted between fourth a…

    Expression

    Mammalian

    Available Since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. Myc-gamma3 (in pCAGGS)

    Plasmid

    #118042

    Purpose

    GABAA receptor expression (gamma3 subunit) Myc-tag near N-terminus

    Depositor

    Angel de Blas

    Insert

    GABRG3 (Gabrg3 Rat)
    

    Tags

    cMyc epitope EQKLISEEDL inserted between fourth a…

    Expression

    Mammalian

    Available Since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. POT5-TEF2p-ycrtYB-ADH1t

    Plasmid

    #65359

    Purpose

    TU for combinatorial construction of ?-carotene pathway

    Depositor

    Junbiao Dai

    Article

    Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

    Insert

    TEF2p-ycrtYB-ADH1t
    

    Use

    Unspecified

    Available Since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. POT2-TEF2p-ycrtE-ADH1t

    Plasmid

    #65353

    Purpose

    TU for combinatorial construction of ?-carotene pathway

    Depositor

    Junbiao Dai

    Article

    Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

    Insert

    TEF2p-ycrtE-ADH1t
    

    Use

    Unspecified

    Available Since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge