We narrowed to 19,278 results for: CAL

Showing: 4501 - 4520 of 19278 results

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TDG004_pLenti_CMVtight_Kir2.1_CFP

Plasmid

#178820

Purpose

tet-on expression of an inward-rectifier potassium channel Kir2.1 carrying an CFP tag

Depositor

Adam Cohen

Article

Tian et al Nat Methods. 2023 Jul;20(7):1082-1094. d

Insert

Kir2.1_CFP (Kcnj2 Mouse)

Use

Lentiviral

Tags

CFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2022

Availability

Academic Institutions and Nonprofits only

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POLArISact

Plasmid

#164971

Purpose

Expresses POLArISact, an orientation probe that specifically binds to F-actin, in mammalian cells

Depositor

Sumio Terada

Article

Sugizaki et al Proc Natl Acad Sci U S A. 2021 Mar 16;11

Insert

POLArISact

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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Lenti_PGK-Cas9-P2A-HygR

Plasmid

#164135

Purpose

Lentiviral construct for the expression of SpCas9 driven by PGK promoter in mammalian cells

Depositor

Hyongbum Kim

Article

Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

Insert

SpCas9 (cas9 Synthetic)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pHR-CMV-TetO2_3C-Avi-His6_IRES-mTurquoise2

Plasmid

#113890

Purpose

Mammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of cytosolic mTurquoise2

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Type

Empty backbone

Use

Lentiviral

Tags

3C-Avi-His6

Promoter

CMV-MIE-TetO2

Available Since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pDisplay-Gncp-iGluSnFR

Plasmid

#107337

Purpose

Noncircularly permutated (ncp) variant of iGluSnFR. Membrane-displayed.

Depositor

Robert Campbell

Article

Wu et al ACS Chem Biol. 2018 Jan 22. doi: 10.1021

Insert

GltI based and noncircularly permutated glutamate biosensor

Expression

Mammalian

Promoter

CMV

Available Since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-H-RAN-GFP1

Plasmid

#106418

Purpose

H-RAN-GFP1 (RANbody GFP1::Sm-HAtag)

Depositor

Joshua Sanes

Article

Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

Insert

H-RAN-GFP1

Tags

HA, His, and Multiple HA

Expression

Mammalian

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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LITE1.0_pAAV_hSyn_TALE(Grm2)-NLS-CIB1_2A_GFP_WPRE_bGHpA

Plasmid

#47453

Purpose

epiLITE / LITE1.0 TALE-CIB1. CIB1 binds to blue-light-activated CRY2PHR. Particular TALE targeted to Grm2 promoter. Synapsin promoter for neuronal expression.

Depositor

Feng Zhang

Article

Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

Insert

TALE (N136,Grm2,C63)-cib1

Use

AAV; Tale

Tags

HA

Expression

Mammalian

Promoter

hSyn human synapsin (mammalian neuronal expressio…

Available Since

Oct. 22, 2013

Availability

Academic Institutions and Nonprofits only

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pDONR223-PRKCA

Plasmid

#23550

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PRKCA (PRKCA Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
TRPML1-MYC

Plasmid

#18824

Depositor

Craig Montell

Article

Venkatachalam et al J Biol Chem. 2006 Jun 23. 281(25):17517-

Insert

TRPML1 (MCOLN1 Human)

Tags

Myc

Expression

Mammalian

Available Since

July 14, 2008

Availability

Academic Institutions and Nonprofits only

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pCS2+ MT Beta Catenin (Xenopus)

Plasmid

#13435

Depositor

Marc Kirschner

Article

Lee et al J Cell Biol. 2001 Sep 3. 154(5):983-93.

Insert

Beta Catenin (ctnnb1.L Frog)

Tags

Myc Tag

Expression

Mammalian

Available Since

Nov. 1, 2006

Availability

Academic Institutions and Nonprofits only
pBMN mCherry-TAX1BP1(ΔUBD)

Plasmid

#119689

Purpose

For mammalian expression of TAX1BP1 without its UBD

Depositor

Michael Lazarou

Article

Padman et al Nat Commun. 2019 Jan 24;10(1):408. doi:

Insert

TAX1BP1 (TAX1BP1 Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Mutation

UBD deletion (1-755)

Available Since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1_MeCP2(deltaNC)

Plasmid

#110190

Purpose

Expression of mouse MeCP2 (deltaNC) in mammalian cells

Depositor

Adrian Bird

Article

Tillotson et al Nature. 2017 Oct 19;550(7676):398-401. d

Insert

MeCP2_e2 delta NC (mouse cDNA) - EGFP (Mecp2 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

delta N and C termini

Promoter

CMV

Available Since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-DIO ChRmine-oScarlet-KV 2.1-WPRE

Plasmid

#183536

Purpose

Optogenetics

Depositor

Karl Deisseroth

Article

Fan et al Cell. 2023 Feb 2;186(3):543-559.e19. doi

Insert

ChRmine-oScarlet-Kv2.1

Use

AAV

Mutation

None

Promoter

CaMKIIa

Available Since

Feb. 9, 2023

Availability

Academic Institutions and Nonprofits only

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CXCR7-DuET

Plasmid

#213224

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

CXCR7 (ACKR3 Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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H6GB1tevP53SS(1-393)

Plasmid

#200311

Purpose

Bacterial expression of human superstable p53 as His6GB1tev fusion

Depositor

Peter Wright

Article

Krois et al Biochemistry. 2022 Dec 6;61(23):2709-271

Insert

p53 (TP53 Human)

Tags

His6-GB1-tev

Expression

Bacterial

Mutation

M133L, V203A, N239Y, N268D

Promoter

T7

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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ALDH18A1

Plasmid

#155402

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

ALDH18A1 (ALDH18A1 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX neo ACTN1-GFP

Plasmid

#138293

Purpose

Mammalian Expression, Lentiviral. Expression of human alpha-actinin-1-GFP fusion protein

Depositor

Gaudenz Danuser

Article

Park et al Nature. 2020 Feb 12. pii: 10.1038/s41586

Insert

ACTN1 (ACTN1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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px459 sgAtg5

Plasmid

#175023

Purpose

CRISPR-Cas9 plasmid targeting exon 2 of human Atg5.

Depositor

David Tumbarello

Article

Assar et al Front Cell Dev Biol. 2020 Aug 4;8:733. d

Insert

ATG5 (ATG5 Human)

Expression

Mammalian

Available Since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-AktAR2-NLS

Plasmid

#175012

Purpose

Nuclear-targeted AktAR2.

Depositor

Jin Zhang

Article

Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

Insert

AktAR-NLS

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pDendra2-H2b

Plasmid

#157817

Purpose

Fluorescent reporter for histone H2B tracking

Depositor

Xin Chen

Article

Ma et al Cell Rep. 2020 Aug 11;32(6):108003. doi:

Insert

Histone H2B (H2bc3 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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