Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

Filter By

We narrowed to 8,304 results for: construct

Showing: 4501 - 4520 of 8304 results

  1. pUC19-E6KTN8

    Plasmid
    #103076
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Article
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    E6KTN8(Cas9 coding gene from Actinomyces sp. oral taxon 180 str. F0310)
    Use
    CRISPR; Cloning vector
    Tags
    Expression
    Mutation
    human codon-optimized
    Promoter
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103076-plasmid-map-sequence-id-201715

    Enlarge

  2. pUC19-A1IQ68

    Plasmid
    #103080
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Article
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    A1IQ68(Cas9 coding gene from Neisseria meningitidis serogroup A / serotype 4A (strain Z2491))
    Use
    CRISPR; Cloning vector
    Tags
    Expression
    Mutation
    human codon-optimized
    Promoter
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103080-plasmid-map-sequence-id-201720

    Enlarge

  3. pUC19-A5EIM8

    Plasmid
    #103081
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Article
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    A5EIM8(Cas9 coding gene from Bradyrhizobium sp. (strain BTAi1 / ATCC BAA-1182))
    Use
    CRISPR; Cloning vector
    Tags
    Expression
    Mutation
    human codon-optimized
    Promoter
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103081-plasmid-map-sequence-id-201721

    Enlarge

  4. pUC19-A7HP89

    Plasmid
    #103082
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Article
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    A7HP89(Cas9 coding gene from Parvibaculum lavamentivorans (strain DS-1 / DSM 13023 / NCIMB 13966))
    Use
    CRISPR; Cloning vector
    Tags
    Expression
    Mutation
    human codon-optimized
    Promoter
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103082-plasmid-map-sequence-id-201722

    Enlarge

  5. pUC19-B5JU35

    Plasmid
    #103087
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Article
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    B5JU35(Cas9 coding gene from gamma proteobacterium HTCC5015)
    Use
    CRISPR; Cloning vector
    Tags
    Expression
    Mutation
    human codon-optimized
    Promoter
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103087-plasmid-map-sequence-id-201737

    Enlarge

  6. pUC19-B5XLC1

    Plasmid
    #103088
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Article
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    B5XLC1(Cas9 coding gene from Streptococcus pyogenes serotype M49 (strain NZ131))
    Use
    CRISPR; Cloning vector
    Tags
    Expression
    Mutation
    human codon-optimized
    Promoter
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103088-plasmid-map-sequence-id-201738

    Enlarge

  7. pUC19-C4ZA16

    Plasmid
    #103090
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Article
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    C4ZA16(Cas9 coding gene from Eubacterium rectale (strain ATCC 33656 / VPI 0990))
    Use
    CRISPR; Cloning vector
    Tags
    Expression
    Mutation
    human codon-optimized
    Promoter
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103090-plasmid-map-sequence-id-201740

    Enlarge

  8. KS6A1_HUMAN_D1

    Plasmid
    #79720
    Purpose
    This plasmid encodes the kinase domain of KS6A1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
    Depositor
    John Chodera, Markus Seeliger, Nicholas Levinson
    Article
    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/
    Insert
    KS6A1 (RPS6KA1 Human)
    Use
    Tags
    His10-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 21, 2016
    Availability
    Academic Institutions and Nonprofits only

  9. KC1G3_HUMAN_D0

    Plasmid
    #79685
    Purpose
    This plasmid encodes the kinase domain of KC1G3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
    Depositor
    John Chodera, Markus Seeliger, Nicholas Levinson
    Article
    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/
    Insert
    KC1G3 (CSNK1G3 Human)
    Use
    Tags
    His10-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 21, 2016
    Availability
    Academic Institutions and Nonprofits only

  10. 1203A-CMV-5’DR-EGFP-bGHpolyA

    Plasmid
    #221452
    Purpose
    EGFP reporter construct with single unprocessed direct repeat (35nt) at 5' end
    Depositor
    Omar Akbari
    Insert
    eGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    221452-plasmid-map-sequence-id-437434

    Enlarge

  11. 1203B-CMV-3’DR-EGFP-bGHpolyA

    Plasmid
    #221453
    Purpose
    EGFP reporter construct with single unprocessed direct repeat (35nt) at 3' end
    Depositor
    Omar Akbari
    Insert
    eGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    221453-plasmid-map-sequence-id-437437

    Enlarge

  12. pIVTR(T7G)-PuroR-F2A-mClover3

    Plasmid
    #172291
    Purpose
    In vitro transcription of PuroR-F2A-mClover3 synthetic construct
    Depositor
    Patrik Verstreken
    Insert
    PuroR-F2A-mClover3
    Use
    Other
    Tags
    Expression
    Mutation
    Promoter
    T7 promoter G-initiating
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    172291-plasmid-map-sequence-id-340310

    Enlarge

  13. pANY4

    Plasmid
    #164897
    Purpose
    Two valuable constructs for either IPTG-induced or temperature-induced expression can be simultaneously obtained in a single cloning process by utilizing plasmid pANY4.
    Depositor
    Yingfeng An
    Insert
    ccdB cassette
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    164897-plasmid-map-sequence-id-327986

    Enlarge

  14. pNP172

    Plasmid
    #106363
    Purpose
    To insert a single copy of a construct at a site on Chr II, corresponding to mKate expressed specifically in the epidermis; can be used for N- or C-terminal fusion.
    Depositor
    Jonathan Ewbank
    Insert
    ttTi5605_SEC_col-12p_mkate2 x _3'tbb-2
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    July 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    106363-plasmid-map-sequence-id-211528

    Enlarge

  15. MZB209

    Plasmid
    #228492
    Purpose
    BirA encoded pACYC vector for co-expression with Avi-tagged constructs (Bacterial Expression)
    Depositor
    Richard Baker
    Insert
    BirA (birA )
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7 Promoter
    Available since
    Jan. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228492-plasmid-map-sequence-id-455391

    Enlarge

  16. pGL3-U6-XsgRNA-ccdB-EF1a-Puromycin

    Plasmid
    #115519
    Purpose
    To construct multiple sgRNA expression vector for CRISPR
    Depositor
    Xingxu Huang
    Insert
    ccdB-sgRNA scaffold
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    115519-plasmid-map-sequence-id-224555

    Enlarge

  17. pCASCADE

    Plasmid
    #65821
    Purpose
    Control guide RNA plasmid E. coli , low phosphate induction
    Depositor
    Michael Lynch
    Insert
    control guideRNA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    E . coli ugpB gene promoter, low phosphate induc…
    Available since
    July 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    65821-plasmid-map-sequence-id-111971

    Enlarge

  18. pBsd-P2a-Fkbp-3xF-GGSG

    Plasmid
    #235514
    Purpose
    N-term tagging construct: clone into ZraI and AfeI sites
    Depositor
    Brian Hendrich
    Insert
    FKBP
    Use
    Mouse Targeting
    Tags
    Bsd, FKBP, 3xFLAG
    Expression
    Mutation
    None
    Promoter
    Available since
    June 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    235514-plasmid-map-sequence-id-473990

    Enlarge

  19. pGGSG-FKBP-2HA-P2a-Pac

    Plasmid
    #235521
    Purpose
    C-term tagging construct: clone into PmlI and AfeI sites
    Depositor
    Brian Hendrich
    Insert
    FKBP
    Use
    Mouse Targeting
    Tags
    FKBP, 2xHA, Puro
    Expression
    Mutation
    None
    Promoter
    Available since
    May 29, 2025
    Availability
    Academic Institutions and Nonprofits only
    235521-plasmid-map-sequence-id-474041

    Enlarge

  20. pBsd-T2a-Fkbp-3xF-GGSG

    Plasmid
    #235515
    Purpose
    N-term tagging construct: clone into ZraI and AfeI sites
    Depositor
    Brian Hendrich
    Insert
    FKBP
    Use
    Mouse Targeting
    Tags
    Bsd, FKBP, 3xFLAG
    Expression
    Mutation
    None
    Promoter
    Available since
    May 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    235515-plasmid-map-sequence-id-474007

    Enlarge

Showing: 4501 - 4520 of 8304 results