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We narrowed to 6,765 results for: sas

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Showing: 4501 - 4520 of 6765 results

  2. pAAV-CMV-dSa VP64 Hbb

    Plasmid
    #99693
    Purpose
    Expresses dSa Cas9 fused to gold standard VP64 activator domain and Sa sgRNA for Hbb promoter, vector allows for activation of mouse Hbb, can be packaged and delivered as AAV
    Depositor
    George Church
    Article
    Vora et al bioRxiv 2018. 298620
    Insert
    dCas9 and gRNA targeting Hbb (gRNA: GGGGTAAGGGGAGCAAGGTC) (Hbb Mouse, Synthetic)
    Use
    AAV and CRISPR
    Tags
    VP64
    Mutation
    dead Cas9
    Available Since
    May 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    99693-plasmid-map-sequence-id-194561

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  7. BPK2101

    Plasmid
    #65770
    Purpose
    Bacterial expression plasmid for S. aureus Cas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSaCas9-NLS-3xFLAG-T7-BsaIcassette-Sa-sgRNA
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized SaCas9, and SaCas9 gRNA
    Use
    CRISPR
    Tags
    NLS-3xFlag
    Expression
    Bacterial
    Promoter
    T7 (x2)
    Available Since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    65770-plasmid-map-sequence-id-112650

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  8. pAAV-SCP1-dSa VPR mini.-2X snRP-1 Hbb

    Plasmid
    #99692
    Purpose
    Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Hbb, vector allows for strong activation of mouse Hbb, can be packaged and delivered as AAV
    Depositor
    George Church
    Article
    Vora et al bioRxiv 2018. 298620
    Insert
    dCas9 and gRNA targeting Hbb (Hbb Synthetic)
    Use
    AAV
    Tags
    VPR mini
    Mutation
    dead Cas9
    Available Since
    Jan. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    99692-plasmid-map-sequence-id-194594

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  14. pONSY-CoH2B:Venus

    Plasmid
    #111877
    Purpose
    This plasmid expresses Venus fluorescent protein fused to endogenous Histone H2B (H2B) gene of Capsaspora (CAOG_01818). It can be used to transfect Capsaspora cells and visualize nucleus in vivo.
    Depositor
    Inaki Ruiz-Trillo
    Article
    Parra-Acero et al Development. 2018 May 11. pii: dev.16210
    Insert
    Capsaspora Histone H2B (CoH2B) fused to Venus
    Use
    Capsaspora owczarzaki
    Tags
    Venus
    Promoter
    Elongation Factor 1 alpha (EF1a) from Capsaspora
    Available Since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    111877-plasmid-map-sequence-id-213489

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  19. pJP102

    Plasmid
    #176480
    Purpose
    Expresses mScarlet under control of the Capsaspora EF1 promoter and NatR under control of the Capsaspora actin promoter
    Depositor
    Duojia Pan
    Article
    Phillips et al Elife. 2022 Jun 6;11. pii: 77598. doi: 1
    Inserts
    actin > NatR
    EF1 > mScarlet
    Use
    Capsaspora owczarzaki expression
    Available Since
    June 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    176480-plasmid-map-sequence-id-346562

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  20. pJP103

    Plasmid
    #176481
    Purpose
    Expresses mScarlet under control of the Capsaspora EF1 promoter and HygR under control of the Capsaspora actin promoter
    Depositor
    Duojia Pan
    Article
    Phillips et al Elife. 2022 Jun 6;11. pii: 77598. doi: 1
    Inserts
    actin > HygR
    EF1 > mScarlet
    Use
    Capsaspora owczarzaki expression
    Available Since
    June 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    176481-plasmid-map-sequence-id-346559

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Showing: 4501 - 4520 of 6765 results