We narrowed to 24,019 results for: Nov
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Plasmid#186553PurposeNegative Control mediating endosomal localizationDepositorInsert10R-8His-EGFP
UseTags8xHisExpressionBacterialMutationPromoterT7Available sinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSHS306 - Bacterial expression plasmid for SpCas9, HNH FRET variant
Plasmid#87350PurposeS867C/S355C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, HNH-1 FRET constructDepositorInsertCas9
UseTagsHis-MBPExpressionBacterialMutationC80S/S355C/C574S/S867CPromoterT7Available sinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
SARS-CoV-2_N_deltaNIDR
Plasmid#157868Purposeexpress MBP tagged SARS-CoV-2 Nucleocapsid protein without the N-terminal IDRDepositorInsertN (N SARS-CoV-2)
UseTagsMBPExpressionBacterialMutationdeltaNIDRPromoterAvailable sinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYFAC-riboB
Plasmid#168975PurposeFilamentous fungi vector with AMA1; 2µ replicon; URA3; AmpR ; riboBDepositorTypeEmpty backboneUseSynthetic Biology; Expression in aspergillus nidu…TagsExpressionMutationPromoterAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-T2A-Nluc-3xP3-RFP
Plasmid#127563PurposeCRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Nluc-SV40 and 3xP3-RFP visible eye marker.DepositorInsertNluc
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCytERM-LSSmGFP
Plasmid#186540PurposeVisualization of the ER in cells using LSSmGFP (ex/em = 400/510 nm); compatible with OSER assay.DepositorInsertLSSmGFP
UseTagsCytochrome p450 first 29 animo acids (Oryctolagus…ExpressionMammalianMutationhfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206KPromoterCMVAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
WFS1 P724L-Flag-pcDNA3
Plasmid#13012DepositorInsertWolfram Syndrome 1 (WFS1 Human)
UseTagsFlagExpressionMammalianMutationchanged P 724 to LPromoterAvailable sinceSept. 26, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCK760
Plasmid#192643PurposeExpresses BBa_J23110-sfGFP (capacity monitor) and J3-Bba_J23117-mRFP (CRISPRa reporter) on pSC101-AmpRDepositorInsertBBa_J23110-sfGFP
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterBBa_J23110Available sinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAGM37443
Plasmid#153218PurposeLevel 2 binary vector for plant transformation; for cloning Cas9 and guide RNAs; single copy in Agrobacterium.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC19-U6-AasgRNA
Plasmid#121954PurposeMammalian expression, Genome editing, gRNA scaffoldDepositorInsertAaCas12b single chimeric gRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKH163_pSmart-mNEON-YIPF4
Plasmid#214982Purposeendogenous mneon tag for YIPF4DepositorInsertmNEON with YIPF4 homology (YIPF4 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSCT-GAL93-PSPC1
Plasmid#46321DepositorInsertPSPC1 (PSPC1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLN342 (FuGW-SSX1p-mKate2)
Plasmid#105172PurposeSSX1 promoter drives mKate2 expressionDepositorInsertSSX1p-mKate2
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFPL-Cg
Plasmid#61652PurposeGateway vector for generating cyan fluorescent protein fusions in fungiDepositorInsertenhanced cyan fluorescent protein
UseTagsExpressionPlantMutationPromoterAvailable sinceMarch 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pALS343 (pGEX-2TP-cTevHis-Gibson/MCS)
Plasmid#139788PurposeVector for recombinant protein expression in E. coli; PreScission-cleavable N-terminal GST and TEV-cleavable C-terminal 6xHisDepositorTypeEmpty backboneUseTags6xHis (cleavable by TEV protease) and GST (cleava…ExpressionBacterialMutationPromoterAvailable sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMP4516
Plasmid#107775PurposeExpression of ECFPDepositorInsertpLacZ-ECFP in pBBR1-mcs5
UseTagspLacZ-ECFPExpressionBacterialMutationPromoterpLacZAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJM126
Plasmid#49339Purposebacterial expression of RFA1, RFA2, and RFA3DepositorUseTagsExpressionBacterialMutationPromoterT7Available sinceJan. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pT7-agrBD-I
Plasmid#53439PurposeT7-dependent expression of the type-I agrB and agrD genes of S. aureus RN4220DepositorInsertagrBD locus
UseSynthetic BiologyTagsV5 epitope, 6x HIS (not expressed on agrB or agrD…ExpressionBacterialMutationnonePromoterT7-lacAvailable sinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRN3P_T3_BE3_IVT
Plasmid#177015PurposePlasmid to be used as DNA template for in-vitro RNA transcription of the BE3 base editor (C to T) by T3 RNA polymeraseDepositorInsertBE3
UseCRISPR; Vector for in-vitro transcriptionTagsExpressionMutationPromoterT3 promoterAvailable sinceDec. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH1924
Plasmid#179205PurposePnmr-1::UNC-7-UrSL-wCherry unc-54 3' UTR C.elegans neural expression of UNC-7-wCherryDepositorInsertUNC-7
UseTagswCherryExpressionWormMutationPromoterPnmr-1Available sinceFeb. 28, 2022AvailabilityAcademic Institutions and Nonprofits only