We narrowed to 29,515 results for: REP
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Plasmid#186753PurposeA basic cloning vector for inserting genes with 5' and 3' UTRs for later subcloning via Golden Gate assembly.DepositorTypeEmpty backboneUseSynthetic Biology; Cloning backboneTagsExpressionMutationPromoterT7Available sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only
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pAAV-EF1a-double floxed-Gat1(R69K)-HA-WPRE-HGHpA
Plasmid#184636PurposeExpresses the GABA membrane transporter Gat1 R69K mutant fused to HA, driven by the Ef1a promoter, in a Cre-dependent fashionDepositorInsertGat1 (Slc6a1 Mouse)
UseAAVTagsExpressionMutationGat1 gene with Arginine 69 changed to Lysine, to …PromoterEF1aAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT2h70CRET(#325)
Plasmid#184060Purposecyclofen-inducible CRE activation in zebrafish transient transgenicDepositorInsertCRE-ERT2
UseZebrafish transient transgenesisTagsExpressionMutationERT2: G400V, M543A, L544A, deleted 1-281;PromoterZf-Hsp70-4Available sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-mCherry-SynaptoZip
Plasmid#177318PurposeAAV expression of the synaptic activity-marker SynaptoZip fused to mCherry driven by human Synapsin promoterDepositorInsertmCherry-SynaptoZip (Vamp2 Rat)
UseAAVTagsMyc and mCherryExpressionMammalianMutationPromoterhSynAvailable sinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pN1mcherry-NKD2G2A
Plasmid#174334Purposeencodes human NKD2 G2A (2nd Gly residue to Ala) mutant cDNA with a C-terminal mCherry fusion.DepositorInsertHomo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
UseTagsmCherry tagExpressionMammalianMutationmutation of a.a 2 Gly to AlaPromoterCMVAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pN1mcherry-NKD2EFMUT
Plasmid#174333Purposeencodes human NKD2 132DFD134>AFA mutant (two D mutated to A)cDNA with a C-terminal mCherry fusion.DepositorInsertHomo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
UseTagsmCherry tagExpressionMammalianMutationNKD2132DFD134>AFA (substitutions of D132 and D…PromoterCMVAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
FG11F-C-FLAG-hNKD2
Plasmid#174330Purposeencodes human NKD2 cDNA with a C-terminal FLAG tag in lentiviral vector. Does not encode any fluorescence/resistance marker.DepositorInsertHomo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
UseLentiviralTagsFLAG TagExpressionMammalianMutationPromoterUbCAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
MO70-C-FLAG-hNKD2
Plasmid#174327Purposeencodes human NKD2 cDNA with a C-terminal FLAG tag in retroviral expression vector. Encodes GFP from IRES site and puromycin resistanceDepositorInsertHomo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
UseRetroviralTagsFLAG TagExpressionMammalianMutationPromoterCMVAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4TO-3XFLAG Halo R99A nsp1
Plasmid#175422PurposeExpresses R99A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.DepositorInsertCoV2 R99A nsp1
UseTags3XFLAG HaloExpressionMammalianMutationPromoterCMVAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Bmpr2 gRNA resistant
Plasmid#163408PurposeExpresses BMPR-2 resistant to the gRNAs(#163388-90) in mammalian cellsDepositorInsertBmpr2 (Bmpr2 Mouse)
UseTagsExpressionMammalianMutationgRNA-targeting sequences are substituted with nuc…PromoterAvailable sinceAug. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP_A893E-talin1 (1-2541)
Plasmid#166116PurposeFor expression of full-length mouse talin-1 carrying mutation A893E in mammalian cells. Contains C-terminal EGFP tag.DepositorInsertA893E-Talin-1 (Tln1 Mouse)
UseCmv promoterTagsEGFPExpressionMammalianMutationA893EPromoterAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP_L1539P-talin1 (1-2541)
Plasmid#166119PurposeFor expression of full-length mouse talin-1 carrying mutation L1539P in mammalian cells. Contains C-terminal EGFP tag.DepositorInsertL1539P-Talin-1 (Tln1 Mouse)
UseCmv promoterTagsEGFPExpressionMammalianMutationL1539PPromoterAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP_L2509P-talin1 (1-2541)
Plasmid#166123PurposeFor expression of full-length mouse talin-1 carrying mutation L2509P in mammalian cells. Contains C-terminal EGFP tag.DepositorInsertL2509P-Talin-1 (Tln1 Mouse)
UseCmv promoterTagsEGFPExpressionMammalianMutationL2509PPromoterAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP_S1750F-talin1 (1-2541)
Plasmid#166120PurposeFor expression of full-length mouse talin-1 carrying mutation S1750F in mammalian cells. Contains C-terminal EGFP tag.DepositorInsertS1750F-Talin-1 (Tln1 Mouse)
UseCmv promoterTagsEGFPExpressionMammalianMutationS1750FPromoterAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP_D2086V-talin1 (1-2541)
Plasmid#166122PurposeFor expression of full-length mouse talin-1 carrying mutation D2086V in mammalian cells. Contains C-terminal EGFP tag.DepositorInsertD2086V-Talin-1 (Tln1 Mouse)
UseCmv promoterTagsEGFPExpressionMammalianMutationD2086VPromoterAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP_R1368W-talin1 (1-2541)
Plasmid#166117PurposeFor expression of full-length mouse talin-1 carrying mutation R1368W in mammalian cells. Contains C-terminal EGFP tag.DepositorInsertR1368W-talin1-Talin-1 (Tln1 Mouse)
UseCmv promoterTagsEGFPExpressionMammalianMutationR1368WPromoterAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP_Y1389C-talin1 (1-2541)
Plasmid#166118PurposeFor expression of full-length mouse talin-1 carrying mutation Y1389C in mammalian cells. Contains C-terminal EGFP tag.DepositorInsertY1389C-Talin-1 (Tln1 Mouse)
UseCmv promoterTagsEGFPExpressionMammalianMutationY1389CPromoterAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-EGxxFP-PIGA
Plasmid#153958PurposeUsed for EGxxFP assay with PIGA intron 5 and exon 6 sequencesDepositorInsertPIGA (a 401-bp fragment from intron 5 and exon 6)
UseA plasmid specific for egxxfp assayTagsN/AExpressionMammalianMutationNo mutationPromoterCAG promoterAvailable sinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
Donor-PIGAmut
Plasmid#153548PurposeUsed as a donor vector for the targeted knock-in of a mutation in PIGA exon 6DepositorInsertmutant PIGA (a 1,991-bp fragment from intron 5 and exon 6 with a 3-bp mutation in exon 6)
UseDonor plasmidTagsN/AExpressionMutationa 3-bp mutation in exon 6PromoterNo promoterAvailable sinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
GFP-Miro1 (1-177 fused to 562-618)
Plasmid#127627PurposeFirst GTPase domain of human Miro1 fused to C-terminusDepositorInsertMiro1 (RHOT1 Human)
UseTagseGFPExpressionMammalianMutation1-177 fused to 562-618PromoterAvailable sinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only