We narrowed to 31,051 results for: SON

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CMV_Npu-ABEmax N-terminal

Plasmid

#137173

Purpose

Expresses the N-terminal split-intein fragment of ABEmax from the CMV promoter

Depositor

David Liu

Article

Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

Insert

Npu-ABEmax_N-terminal

Expression

Mammalian

Mutation

Cas9 D10A

Promoter

CMV

Available Since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pET_RGG-RGG-RGG

Plasmid

#124938

Purpose

Expression of RGG-RGG-RGG

Depositor

Matthew Good, Daniel Hammer, Benjamin Schuster

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

RGG-RGG-RGG (using LAF-1 RGG domain) (laf-1 Nematode)

Tags

His

Expression

Bacterial

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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Gata4-GFP donor

Plasmid

#113115

Purpose

Donor plasmid for knock-in eGFP fusing to the c-terminal of mouse Gata4 coding sequence

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

Gata4 donor region including homology arms and eGFP

Use

Synthetic Biology

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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BICDN-CIBN

Plasmid

#102253

Purpose

Expresses fusion of dynein adapter BICDN (1-594) and CIB1 (1-170)

Depositor

Bianxiao Cui

Article

Duan et al Chem Biol. 2015 May 21;22(5):671-82. doi

Insert

BICDN-CIB1 (CIB1 Mustard Weed)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pZac2.1 EFs-SaCas9-U6-Sa DMDR7-U6-SaDMDL2

Plasmid

#78607

Purpose

A single vector AAV-Cas9 system containing SaCas9 under EFs promoter, gRNAs targeting Dmd introns 22 and 23

Depositor

Amy Wagers

Article

Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

Insert

EFs-SaCas9-U6-Sa DMDR7-U6-SaDMDL2

Use

AAV and CRISPR

Tags

HA tag

Expression

Mammalian

Promoter

EF1a-short; U6

Available Since

Sept. 16, 2017

Availability

Academic Institutions and Nonprofits only
pCrysβ:ECFP-LexOP:mCherry-KrasG12V

Plasmid

#89888

Purpose

Human KRasG12V fused to mCherry downstream of LexOP, placed within a non-autonomous maize dissociation (Ds) element for integration in zebrafish. Contains pCrysB-ECFP for screening.

Depositor

Tatjana Sauka-Spengler

Article

Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

Insert

mCherry-KRasG12V

Use

Unspecified

Tags

mCherry

Available Since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pADtet-His6-MscL

Plasmid

#83373

Purpose

Expresses wild type MscL in mammalian cells

Depositor

Allen Liu

Article

Heureaux et al Cell Mol Bioeng. 2014 Sep;7(3):307-319.

Insert

Mechanosensitive channel of large conductance (mscL E. coli)

Use

Adenoviral

Tags

His6 tag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 28, 2016

Availability

Academic Institutions and Nonprofits only
mCherry-ALK1-C-13

Plasmid

#54968

Purpose

Expresses mCherry-ALK1 in mammalian cells.

Depositor

Michael Davidson

Insert

ALK1 (ACVRL1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only
SpCas9-NG-YE1-BE4max

Plasmid

#198535

Purpose

Expresses human codon-optimized SpCas9-NG-YE1-BE4max and blasticidin resistance: EFS promoter-SpCas9-NG-YE1-BE4max-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

Insert

SpCas9-NG-YE1-BE4max

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Promoter

EFS

Available Since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pNS35 (multiAsCas12a piggyBac)

Plasmid

#217336

Purpose

piggyBac expression of multiAsCas12a

Depositor

Luke Gilbert

Article

Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

Insert

multiAsCas12a

Use

CRISPR

Tags

6xMycNLS and HA-SV40NLS-P2A-TagBFP2

Expression

Mammalian

Mutation

R1226A/E174R/S542R/K548R

Promoter

CAG

Available Since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA-puro-TagBFP-β-ACTIN-3′UTR-24xMS2

Plasmid

#205161

Purpose

express β-ACTIN-3′UTR-24xMS2

Depositor

Weirui Ma

Article

Hu et al Elife. 2023 Mar 3;12:e82178. doi: 10.755

Insert

β-ACTIN-3′UTR-24xMS2

Tags

tagBFP

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pEVOL-NBK-1

Plasmid

#207639

Purpose

A plasmid for the expression of M. mazei pyrrolysine aminoacyl-tRNA synthetase/tRNA pair (PylRS/tRNAPyl) in E. Coli.

Depositor

Cole DeForest

Article

Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

Insert

Pyl-tRNACUA and 2x pyrrolysyl-tRNA synthetase (MmPylRS)

Expression

Bacterial

Mutation

The evolved synthetase NBK-1 contains mutations Y…

Promoter

araBAD

Available Since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pSL5069 (pDonor(AAVS1),CRISPR_Pse)

Plasmid

#200904

Purpose

Encodes a mini-transposon derived from Pseudoalteromonas Tn7016 with a splice acceptor-T2A-PuroR-T2A-eGFP cargo, and a CRISPR RNA under a U6 promoter. Total transposon size = 2,024 bp.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

Mini Tn7016 (AAVS1 NGS), PseCRISPR (tSL425)

Expression

Mammalian

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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HiBiT-FZD7

Plasmid

#195845

Purpose

Expresses human FZD7 with an N-terminal HiBiT

Depositor

Gunnar Schulte

Article

Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

Insert

FZD7 (FZD7 Human)

Tags

HiBiT

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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Td-CBEmax

Plasmid

#196600

Purpose

Mammalian expression of Td-CBEmax

Depositor

Dali Li

Article

Chen et al Nat Biotechnol. 2022 Nov 10. doi: 10.103

Insert

bpNLS-TadA-8e (N46L/E27R)-32aa linker-nSpCas9-bpNLS-P2A-UGI-bpNLS

Tags

6*His

Expression

Mammalian

Mutation

TadA-8e (N46L/E27R); S. pyogenes Cas9 (D10A)

Available Since

March 7, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-EF1alpha-VP64-dCas9-VP64_Blast

Plasmid

#192650

Purpose

3rd generation lenti vector encoding VP64-dCas9-VP64 with 2A Blast resistance marker

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

VP64-dCas9-VP64

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A and N863A in Cas9

Promoter

EF1a

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-EF1alpha-dCas9-KRAB_Hygro

Plasmid

#192663

Purpose

3rd generation lenti vector encoding dCas9-KRAB with 2A Hygro resistance marker

Depositor

Jeffrey Miner

Article

Omachi et al Cell Rep. 2026 Mar 24;45(3):117006. doi:

Insert

dCas9-KRAB

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A, D839A, H840A and N863A in Cas9

Promoter

EF1a

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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HaloTag-ER-3

Plasmid

#186960

Purpose

ER lumen localizaing HaloTag

Depositor

Ke Xu

Article

Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

Insert

CALR (CALR Human)

Tags

HaloTag

Expression

Mammalian

Promoter

CMV Promoter

Available Since

Sept. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pEx-deletion-tet

Plasmid

#172214

Purpose

For allelic exchange in tetracyclin-sensitive Prevotella copri strains

Depositor

Till Strowig

Article

Li et al EMBO J. 2021 Dec 1;40(23):e108287. doi:

Type

Empty backbone

Use

Allelic exchange in prevotella copri

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKS2-TVA-eGFP-N2cG

Plasmid

#175439

Purpose

Cre-on (DIO) helpers for N2c rabies monosynaptic tracing; TVA, eGFP and N2cG under Synpasin promotoer

Depositor

Gordon Fishell

Article

Pouchelon et al Cell Rep. 2021 Nov 9;37(6):109993. doi:

Insert

TVA-P2A-eGFP-P2A-N2cG

Use

AAV and Cre/Lox

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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