We narrowed to 15,987 results for: nes
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Plasmid#169699PurposeTemplate plasmid for generating C-terminal 3xFLAG epitope tag fusions to bacterial genes by "Lambda red" recombineering.DepositorInsert3xFLAG tag adjacent to FRT-flanked aph (KanR) gene
Tags3xFLAG tagExpressionBacterialAvailable SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-SaCas9-U6-sgGrin2b
Plasmid#124851PurposeMutagenesis of Grin2bDepositorInsertGrin2b (Grin2b Mouse)
UseAAV, CRISPR, and Mouse TargetingAvailable SinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1080 - prRosa26v2 LSL FLAG-SpCas9
Plasmid#113163PurposeA donor plasmid with homologous arms matching rat Rosa26 and expressing a Cre-dependent FLAG-tagged SpCas9DepositorInsertSpCas9
UseCRISPRTagsFLAGExpressionMammalianPromoterCAGAvailable SinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOBCol3.6-GFPtpz
Plasmid#110211PurposeLabels immature osteoblasts and connective tissue cells expressing type 1 collagen.DepositorInserteGFPtopaz
TagsNoneExpressionMammalianMutationNonePromoter3.6 kb rat type 1 collagen + first intronAvailable SinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
LeGO-lnc-MONC
Plasmid#86966PurposeExpresses lncRNA MONC (Alias: MIR99AHG; LINC00478)DepositorAvailable SinceFeb. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pECFP-C1-hSERT
Plasmid#70104Purposemammalian expression of hSERT with N-terminal CFP fusionDepositorAvailable SinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-Cep123 (CCDC123/Cep89)
Plasmid#67787PurposeThis plasmid encodes EGFP fused to the N-terminus of isoform 1 of Cep123 (CCDC123/Cep89)DepositorAvailable SinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUAS-hActin-luc2
Plasmid#48081PurposeLuciferase Reporter with Gal4 DNA binding site upstream of hACTB promoterDepositorInsertLuciferase
ExpressionMammalianMutationremoved hsp70 promoter, inserted amplified 615 bp…Available SinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
MLM3709
Plasmid#49943PurposeExpresses a 3x Flag TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding siteDepositorAvailable SinceDec. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
NCD-GFP
Plasmid#24507DepositorAvailable SinceApril 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
DNMT3b KO Neo
Plasmid#16651DepositorInsertDNMT3b knock-out construct (DNMT3B Human)
ExpressionBacterialAvailable SinceMarch 10, 2008AvailabilityAcademic Institutions and Nonprofits only -
pMIG-N19RhoA
Plasmid#14120DepositorInsertN19 Human RhoA (RHOA Human)
UseRetroviralTagsMycMutationSite directed mutagenesis of Thr to Asn at Codon …Available SinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pTBL235 NheI-Cas9-KpnI-GSAlinker-FseI-TdT-NotI
Plasmid#126480PurposeExpresses Cas9-GSAlinker-TdT in mammalian cells.DepositorInsertsExpressionMammalianMutationChanged codons to reduce repetitive sequences.PromoterCMVAvailable SinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLM-AMA15.0 Cas9
Plasmid#138944PurposeFungal AMA1 plasmid with sp-Cas9, ribozymes based "plug-and-play" sgRNA transcription unit, Terbinafine and Phleomycin selection markersDepositorInsertp40S:sp_Cas9_2xNLS; HH-HDV sgRNA transcription unit, Terbinafine (ergA) and Phleomycin (ble) selection markers
UseCRISPR and Synthetic Biology; Ama1 autonomously r…TagsNLSPromoterp40S (AN0465) Aspergillus nidulansAvailable SinceJan. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag GCK
Plasmid#20492DepositorAvailable SinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCAG-HiBiT-CD63
Plasmid#162591PurposeN-terminally HiBiT-tagged human CD63 under CAG promoterDepositorInserthuman CD63 (CD63 Human, Synthetic, human)
TagsHiBiTExpressionMammalianPromoterCAG promoterAvailable SinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
BC1209-pHR-dSV40-dCas9-14xGFP11-P2A-BFP
Plasmid#164038PurposeExpression of dCas9-14xGFP11 in mammalian cellsDepositorInsertdCas9-14xGFP11
UseCRISPR and LentiviralExpressionMammalianMutationD10A and H840A in Cas9PromoterdSV40Available SinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
DDX39B_pLX307
Plasmid#98331PurposeLentiviral expression of DDX39BDepositorInsertDDX39B (DDX39B Human)
UseLentiviralTagsV5ExpressionMammalianMutationS296PPromoterE1FaAvailable SinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-dCas9/MSCV-EGFP
Plasmid#82613PurposeExpresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.DepositorInsert3xFLAG-dCas9
UseRetroviralTags3xFLAG tag and NLS (nuclear localization signal)Mutationhuman codon-optimized, D10A + H840APromoterLTRAvailable SinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-MAP1LC3A
Plasmid#34824DepositorInsertmicrotubule-associated protein 1 light chain 3 alpha (MAP1LC3A Human)
TagsHis and TEVExpressionBacterialAvailable SinceMarch 25, 2013AvailabilityAcademic Institutions and Nonprofits only