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162,619 results

Showing: 4541 - 4560 of 162619 results

  3. mCherry-TGNP-N-10

    Plasmid
    #55145
    Purpose
    Localization: Golgi Complex, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    TGNP (TGOLN2 Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    55145-plasmid-map-sequence-id-339930

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  7. pR26-CMVconst

    Plasmid
    #127373
    Purpose
    Allows for constitutive gene expression from the murine ROSA26 safe harbor locus upon CRISPR/Cas9-mediated genomic insertion and stable selection.
    Depositor
    Lance Miller
    Article
    Routh et al Front Immunol. 2020 Feb 13;11:57. doi: 1
    Type
    Empty backbone
    Use
    CRISPR and Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    CMV enhancer/promoter
    Available since
    Feb. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    127373-plasmid-map-sequence-id-248011

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  13. pSPIH6

    Plasmid
    #190676
    Purpose
    E. coli plasmid for single step cloning of SUMO-peptide-intein (SPI) fusion proteins (contains SUMO and His-tagged intein)
    Depositor
    John Vederas
    Article
    Lamer et al BMC Biotechnol. 2023 Apr 5;23(1):11. doi
    Inserts
    Small ubiquitin-like modifier
    Mxe GyrA intein with C-terminal chitin binding domain and His tag
    Use
    Tags
    Chitin binding domain, His tag, and N-terminal Hi…
    Expression
    Bacterial
    Mutation
    Added C-terminal his tag and None
    Promoter
    T7
    Available since
    Feb. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    190676-plasmid-map-sequence-id-383624

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  14. pM162

    Plasmid
    #173221
    Purpose
    Expresses ScFV-GCN4-GFP10M2-GB1-T2A-OptGFP(1-9)-GB1 and Puro-T2A-MCP-mCherry-GFP11-GB1
    Depositor
    Hajin Kim
    Article
    Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do
    Inserts
    ScFV-GCN4-GFP10M2-GB1-T2A-OptGFP (1-10)-GB1
    MCP-mCherry-GFP11-GB1
    Use
    CRISPR and Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    173221-plasmid-map-sequence-id-341549

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  15. pETM6-G6-vioABE-4A6-vioC

    Plasmid
    #73439
    Purpose
    Deoxyviolacein pathway (vioABEC) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variants G6 (vioABE) and 4A6 (vioC) for orthogonal flux redirection.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    PG6-vioABE-P4A6-vioC
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PG6 and P4A6 (orthogonal T7-lac variants)
    Available since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    73439-plasmid-map-sequence-id-135338

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  17. pCBh_NLS_dCas13X_NLS-pA-U6-DR-BpiI-BpiI-DR-pSV40-EGFP-pA-pSV40-mCherry-pA

    Plasmid
    #191796
    Purpose
    vector for encoding a human codon-optimized dead Cas13X (dCas13X) driven by CBh promoter, guide RNAs compatible with Cas13X driven by hU6, EGFP driven by SV40 promoter, and mCherry driven by SV40 promoter
    Depositor
    Huawei Tong
    Article
    Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103
    Insert
    humanized dCas13X
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    R84A, H89A, R739A, R740A, H745A, H746A, H747A
    Promoter
    CBh, SV40, hU6
    Available since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    191796-plasmid-map-sequence-id-375578

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  18. pTC223

    Plasmid
    #70019
    Purpose
    Cas9/sgRNA targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based on Bean Yellow Dwarf Virus; sgRNA= 7
    Depositor
    Daniel Voytas
    Article
    Cermak et al Genome Biol. 2015 Nov 6;16(1):232. doi:
    Insert
    Nuclease (Cas9/sgRNA) + Donor + GVR
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Dec. 1, 2015
    Availability
    Academic Institutions and Nonprofits only
    70019-plasmid-map-sequence-id-124558

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Showing: 4541 - 4560 of 162619 results