Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

178,151 results

Showing: 4541 - 4560 of 178151 results

  1. pET-PE2-His (IK1822)

    Plasmid
    #170103
    Purpose
    T7 promoter bacterial expression plasmid for PE2 (nSpCas9(H840A)-MMLV-RT) with C-terminal 6xHis-tag and bipartite NLS
    Depositor
    Keith Joung
    Publication
    Petri et al Nat Biotechnol. 2021 Apr 29. pii: 10.103
    Insert
    bpNLS-nSpCas9(H840A)-MMLVRT-bpNLS-6xHis
    Use
    CRISPR
    Tags
    6xHis tag
    Expression
    Bacterial
    Mutation
    Bacteria codon-optimized nSpCas9 (H840A) & M-…
    Promoter
    T7
    Available since
    May 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    170103-plasmid-map-sequence-id-332603

    Enlarge

  4. HDM_SARS2_Spike_del21_D614G

    Plasmid
    #158762
    Purpose
    Mammalian expression vector for expressing SARS-2 Spike with 21 aa del at C-terminus and pt mutation D to G at site 614
    Depositor
    Jesse Bloom
    Publication
    Crawford et al Viruses. 2020 May 6;12(5). pii: v1205051
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SARS2-Spike_del21_D614G (S Synthetic, Severe acute respiratory syndrome coronavirus 2)
    Expression
    Mammalian
    Mutation
    deleted amino acids 1257-1278, changed aspartic a…
    Promoter
    CMV
    Available since
    Aug. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    158762-plasmid-map-sequence-id-309495

    Enlarge

  6. pLVX-EF1alpha-SARS-CoV-2-nsp10-2xStrep-IRES-Puro

    Plasmid
    #141376
    Purpose
    Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus
    Depositor
    Nevan Krogan
    Publication
    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:
    Insert
    SARS-CoV-2-nsp10 (ORF1ab SARS-CoV-2)
    Use
    Lentiviral
    Tags
    2xStrep
    Expression
    Mammalian
    Mutation
    human codon optimized
    Promoter
    EF1alpha
    Available since
    April 9, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    141376-plasmid-map-sequence-id-288095

    Enlarge

  8. pHREAC

    Plasmid
    #134908
    Purpose
    Overexpression of recombinant proteins in plants thanks to very efficient translational efficiency. Empty vector, clone ORF using Bsa1 sites. Download fully annotated map under Resource Information.
    Depositor
    George Lomonossoff
    Publication
    Peyret et al Plant Methods. 2019 Sep 18;15:108. doi:
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Plant
    Promoter
    double 35S
    Available since
    Dec. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    134908-plasmid-map-sequence-id-342556

    Enlarge

  14. pAAV.Syn.GCaMP6f.WPRE.SV40

    Plasmid
    #100837
    Purpose
    AAV expression of ultrasensitive protein calcium sensor from Synapsin promoter
    Depositor
    Douglas Kim, GENIE Project
    Publication
    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d
    Has service
    AAV1, AAV5, and AAV9
    Insert
    GCaMP6f
    Use
    AAV
    Expression
    Mammalian
    Mutation
    GCaMP3-T302L R303P A317E D380Y T381R S383T R392G
    Promoter
    Synapsin
    Available since
    Nov. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    100837-plasmid-map-sequence-id-198307

    Enlarge

  19. pTT5-hSDC1

    Plasmid
    #52326
    Purpose
    expresses human syndecan 1 in HEK293-EBNA1 (293E) suspension culture.
    Depositor
    Gordon Laurie
    Publication
    Zhang et al J Biol Chem. 2013 Apr 26;288(17):12090-1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SDC1 (SDC1 Human)
    Expression
    Mammalian
    Mutation
    R117Q mutation (R95Q according to numbering in Fi…
    Promoter
    CMV
    Available since
    Dec. 8, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    52326-plasmid-map-sequence-id-134443

    Enlarge

Showing: 4541 - 4560 of 178151 results