165,343 results

1. pLHCX-gpASNase1

   Plasmid

   #121526

   Purpose

   Expresses gpASNase1 in mammalian cells.

   Depositor

   Lucas Sullivan, Matthew Vander Heiden

   Article

   Sullivan et al Nat Cell Biol. 2018 Jul;20(7):782-788. d

   Insert

   gpASNase1
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Bacterial and Mammalian

   Promoter

   CMV

   Available Since

   Sept. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSIN-PAmCherry-KFERQ-NE

   Plasmid

   #102365

   Purpose

   Lentiviral overexpression of PA-mCherry-KFERQ fusion

   Depositor

   Shu Leong Ho

   Article

   Ho et al Autophagy. 2019 Apr 14:1-24. doi: 10.108

   Inserts

   PA-mCherry
   KFERQ peptide
   

   Use

   Lentiviral

   Tags

   NE

   Expression

   Mammalian

   Promoter

   EF-1a

   Available Since

   Dec. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. tetO-GFPnls-mPGK-rtTA-IRES-Puro

   Plasmid

   #69545

   Purpose

   Tet-On system expressing GFP tagged with 2x nuclear localization signals.

   Depositor

   Charles Gersbach

   Insert

   eGFP
   

   Use

   Lentiviral

   Tags

   2x nuclear localization sequence and T2A "sk…

   Available Since

   April 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUCmini-iCAP-AAV9-X1.1

   Plasmid

   #196836

   Purpose

   non-standard AAV2 rep-AAV9-X1.1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

   Depositor

   Viviana Gradinaru

   Article

   Chen et al Nat Commun. 2023 Jun 8;14(1):3345. doi:

   Insert

   Synthetic construct isolate AAV9-X1.1 VP1 gene
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   p41

   Available Since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-CMV-EGFP-NLS

   Plasmid

   #165441

   Purpose

   Expresses nuclear EGFP from CMV promoter

   Depositor

   Esteban Engel

   Article

   Maturana et al Gene Ther. 2021 Jun 28. pii: 10.1038/s41

   Insert

   EGFP-NLS
   

   Use

   AAV

   Tags

   NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA HA HP1 beta

   Plasmid

   #24079

   Depositor

   Naoko Tanese

   Article

   Vassallo et al Proc Natl Acad Sci U S A. 2002 Apr 30. 9

   Insert

   HP1 beta (CBX1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Oct. 27, 2010

   Availability

   Academic Institutions and Nonprofits only

7. EGFP-Tau(WT)

   Plasmid

   #187023

   Purpose

   Expresses EGFP tagged human 2N4R tau (WT) in mammalian cells with CMV promoter

   Depositor

   George Bloom

   Article

   Rajbanshi et al Alzheimers Dement. 2023 Jan 12. doi: 10.

   Insert

   MAPT (MAPT Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSLQ9714: pHR-PGK-SV40_NLS-dCasMINI-V4-NZF-c-Myc_NLS-mCherry-WPRE

   Plasmid

   #203220

   Purpose

   Expression of dCasMINI-V4-NZF-mCherry in mammalian cells

   Depositor

   Stanley Qi

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   dCasMINI-V4-NZF-mCherry
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   April 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSpCas9n(BB)-2A-GFP (PX461)

   Plasmid

   #48140

   Purpose

   Cas9n (D10A nickase mutant) from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNA

   Depositor

   Feng Zhang

   Article

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Insert

   hSpCas9n
   

   Use

   CRISPR

   Tags

   3XFLAG and GFP

   Expression

   Mammalian

   Mutation

   Cas9 D10A nickase mutant

   Promoter

   Cbh

   Available Since

   Oct. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. AiP11818-pAAV-AiE2004m_3xC2-minCMV-SYFP2-WPRE3-BGHpA (Alias: CN1818)

    Plasmid

    #164458

    Purpose

    AiE2004m_3xC2 is an optimized enhancer sequence designed to drive AAV-mediated transgene expression in L4/5_IT cortical neurons

    Depositor

    Allen Institute for Brain Science, Jonathan Ting

    Article

    Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

    Insert

    SYFP2
    

    Use

    AAV

    Promoter

    minCMV

    Available Since

    Feb. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGWB-cLUC

    Plasmid

    #174051

    Purpose

    Gateway compatible plasmid containing C-terminal luciferase for split-luciferase complementation assay

    Depositor

    Alberto Macho

    Article

    Yu et al PLoS Pathog. 2020 Sep 25;16(9):e1008933.

    Type

    Empty backbone

    Use

    Luciferase

    Expression

    Plant

    Available Since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGTR

    Plasmid

    #63143

    Purpose

    PCR template to construct polycistronic tRNA-gRNA

    Depositor

    Yinong Yang

    Article

    Xie et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

    Insert

    gRNA-tRNA fusion
    

    Use

    CRISPR and Synthetic Biology

    Available Since

    April 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. plentiCRISPR-v2 Puro EIF2AK3/PERK_sgRNA

    Plasmid

    #218666

    Purpose

    sgRNA targeting human EIF2AK3/PERK

    Depositor

    Kivanc Birsoy

    Article

    Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

    Insert

    EIF2AK3 (EIF2AK3 Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLifeAct-mScarlet3_N1

    Plasmid

    #189767

    Purpose

    Construct for labelling actin filaments in mammalian cells with lifeact-mScarlet3

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    LifeAct-mScarlet3
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR223-MET

    Plasmid

    #23889

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    MET (MET Human)
    

    Use

    Gateway donor vector

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. PB-TRE-EGFP-EF1a-rtTA

    Plasmid

    #104454

    Purpose

    PiggyBac plasmid expressing EGFP under the control of a doxycycline-inducible promotor

    Depositor

    Volker Busskamp

    Article

    Sauter et al Methods Mol Biol. 2019;1942:101-121. doi

    Inserts

    enhanced green fluorescent protein
    reverse tetracycline-controlled transactivator 3
    

    Use

    Piggybac

    Expression

    Mammalian

    Available Since

    April 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. MAP2K2-NanoLuc Fusion Vector

    Plasmid

    #238877

    Purpose

    Express MAP2K2-NanoLuc Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    MAP2K2 (MAP2K2 Human)
    

    Tags

    NanoLuc (R)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. neurod1:Gal4; cmlc2:EGFP

    Plasmid

    #173478

    Purpose

    Gal4 is driven by -5kb neurod1 promoter. The vector contains a cmlc2:EGFP marker

    Depositor

    John Rawls

    Article

    Ye et al Cell Host Microbe. 2021 Feb 10;29(2):179

    Insert

    Gal4
    

    Use

    Zebrafish expression

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGP-AAV-syn-iGABASnFR2-WPRE

    Plasmid

    #218874

    Purpose

    AAV-mediated expression of improved GABA sensor (positive change in fluorescence)

    Depositor

    GENIE Project

    Article

    Kolb et al bioRxiv 2025.03.25.644953

    Has Service

    AAV1 and AAV5

    Insert

    iGABASnFR2
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    S99A F102Y F104Y L178S

    Promoter

    Synapsin

    Available Since

    June 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. IDG_CACNB2_OE_1

    Plasmid

    #161684

    Purpose

    Overexpresses CACNB2

    Depositor

    Mike McManus

    Insert

    CACNB2 (CACNB2 Human)
    

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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