We narrowed to 13,971 results for: EGFP
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Plasmid#222876PurposeRetroviral vector encoding biosensor chains to detect rapamycin (FRB/FKBP domains) and respond with split NanoLuciferase reconstitution (11S/114 fragment). Mixed WT/V84R Glycophorin A (GPA) scaffold.DepositorInsertFRB-GPA(V84R)-NanoLuc114-T2A-FKBP-GPA(WT)-NanoLuc11S
UseRetroviralTagsMycExpressionMammalianMutationFRB chain: V84R in GPAPromoterMSCV LTRAvailable SinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
Dyn1 R271E D291R
Plasmid#198342PurposeMammalian expression plasmid of GFP-chimera proteins.DepositorAvailable SinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
Dyn1 R271E E720R
Plasmid#198344PurposeMammalian expression plasmid of GFP-chimera proteins.DepositorAvailable SinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lnig-INTR-GFP
Plasmid#208904Purposeencodes the inotocin receptor (oxytocin/vasopressin-like) from the ant Lasius niger, tagged with GFPDepositorInsertINTR
TagsEGFPExpressionMammalianAvailable SinceNov. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
Interface-4 (b)
Plasmid#204435PurposeMammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.DepositorAvailable SinceOct. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFSynW SYT9 D197, 199, 330, 332N IRES GFP
Plasmid#195703PurposeLentiviral plasmid encoding SYT9 with D197, 199, 330, 332N mutations followed by an internal ribosomal entry site followed by EGFP under the human synapsin promoterDepositorAvailable SinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
Interface-1 (a)
Plasmid#204428PurposeMammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.DepositorInserthOpa1 isoform 1 (OPA1 Human)
TagsEGFPExpressionMammalianMutationR627A K663A R683APromoterCMVAvailable SinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
Interface-1 (b)
Plasmid#204429PurposeMammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.DepositorInserthOpa1 isoform 1 (OPA1 Human)
TagsEGFPExpressionMammalianMutationE626A T630A K668A E671APromoterCMVAvailable SinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
Interface-1 (c)
Plasmid#204430PurposeMammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.DepositorAvailable SinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
Interface-1 (d)
Plasmid#204431PurposeMammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.DepositorAvailable SinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
Interface-1 (e)
Plasmid#204432PurposeMammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.DepositorAvailable SinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
Interface-2
Plasmid#204433PurposeMammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.DepositorInserthOpa1 isoform 1 (OPA1 Human)
TagsEGFPExpressionMammalianMutationK614A H615A D835APromoterCMVAvailable SinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
Palpha6 and Interface-4 (a)
Plasmid#204434PurposeMammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.DepositorInserthOpa1 isoform 1 (OPA1 Human)
TagsEGFPExpressionMammalianMutationF860A Y861A Y862A Y863A Q864A R865A H866A F867APromoterCMVAvailable SinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
Interface-9
Plasmid#204440PurposeMammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.DepositorInserthOpa1 isoform 1 (OPA1 Human)
TagsEGFPExpressionMammalianMutationE213A Q217A R557A D565APromoterCMVAvailable SinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
Palpha2
Plasmid#204441PurposeMammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.DepositorInserthOpa1 isoform 1 (OPA1 Human)
TagsEGFPExpressionMammalianMutationW771A K772A K773A R774A W775A L776A Y777A W778A K…PromoterCMVAvailable SinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
trp1D-proLYS2-GFP-PKCdelta(C1a+C1b)-NAT
Plasmid#184774PurposeMarker for DAG. Yeast expression of mouse PKCdelta under LSY2 promoter. Uses antibiotic resistance marker natMX6. Replaces endogenous TRP1 upon genome integration, leading to trp1D.DepositorAvailable SinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
OA-1085L
Plasmid#191376PurposepBac-U6-crGFP-array-UTR-opie2-eGFP-3xp3-tdTomatoDepositorInsert4 gRNAs targeting GFP
ExpressionInsectAvailable SinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5-STABLE2-neo-RvCAHS3
Plasmid#192472PurposeStable expression of RvCAHS3 (fly codon optimized) in Drosophila cellsDepositorInsertCAHS3
TagsT2A-EGFP-T2A-neoRExpressionInsectPromoterAc5 promoterAvailable SinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pv6_CBX7_2xChromo-W35A
Plasmid#179397PurposeCell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7_2xChromo(W35A)DepositorInsertCBX7_2xChromo(W35A)
TagsAviTag and EGFPExpressionMammalianMutationChromodomain W35APromoterCAGGSAvailable SinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
PLL5.0-Anillin ShRNA-GFP-AnillinA740D, E758K
Plasmid#187274PurposeLentiviral expression of shRNA targeting Anillin + reconstitution of Anillin A740D,E758KDepositorInsertAnillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra) (ANLN Human)
UseLentiviralTagsEGFPMutationAnillin A740D, E758KPromoterU6, 5'LTRAvailable SinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pv6_CBX1_2xChromo-W42A
Plasmid#179394PurposeCell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX1_2xChromo(W42A)DepositorInsertCBX1_2xChromo(W42A)
TagsAviTag and EGFPExpressionMammalianMutationChromodomain W42APromoterCAGGSAvailable SinceAug. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pv6_CBX7_Chromo_TAF3_Phd-DW890/891AA
Plasmid#179402PurposeCell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7_Chromo - TAF3_Phd(DW890/891AA)DepositorInsertCBX7_Chromo - TAF3_Phd(DW890/891AA)
TagsAviTag and EGFPExpressionMammalianMutationPhd domain DW890/891AAPromoterCAGGSAvailable SinceAug. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pv6_CBX7_Chromo-W35A_TAF3_Phd
Plasmid#179401PurposeCell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7_Chromo(W35A) - TAF3_PhdDepositorInsertCBX7_Chromo(W35A) - TAF3_Phd
TagsAviTag and EGFPExpressionMammalianMutationChromodomain W35APromoterCAGGSAvailable SinceAug. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pv6_MBD1_MBD-R22A
Plasmid#179405PurposeCell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MBD1_MBD(R22A)DepositorInsertMBD1_MBD(R22A)
TagsAviTag and EGFPExpressionMammalianMutationMBD R22APromoterCAGGSAvailable SinceAug. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJET-osr1-KI-donor
Plasmid#184875PurposeDonor template for knock-in of p2a-CreERT2 into the medaka osr1 locus via CRISPR/Cas9-mediated homology directed repairDepositorInsertsOdd-Skipped Related Transcription Factor 1 5' homology arm
Odd-Skipped Related Transcription Factor 1 3' homology arm
p2a-CreERT2
Myosin light chain 2 promoter
EGFP
UseCRISPR and Cre/LoxAvailable SinceJuly 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GFP-IRES-hSNCA (S129D)
Plasmid#185719PurposeAAV expression of GFP and human α-Synuclein with S129D mutation from hSyn promoterDepositorAvailable SinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GFP-IRES-hSNCA (S129A)
Plasmid#185718PurposeAAV expression of GFP and human α-Synuclein with S129A mutation from hSyn promoterDepositorAvailable SinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GFP-IRES-hSNCA (A11P/V70P)
Plasmid#185717PurposeAAV expression of GFP and human α-Synuclein with A11P and V70P mutations from hSyn promoterDepositorInsertsynuclein, alpha (SNCA Human)
UseAAVMutationChanged Ala 11 to Pro, Val 70 to ProPromoterhSynAvailable SinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GFP-IRES-mSNCA(1-95)
Plasmid#185716PurposeAAV expression of GFP and C-terminal truncated (1-95 amino acid) mouse α-Synuclein from hSyn promoterDepositorAvailable SinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX458_sgAgo2_Nterm
Plasmid#170920PurposeExpresses spCas9 and sgRNA targeting the N-terminus of Ago2 for N-terminal taggingDepositorAvailable SinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX458_sgAgo1_Nterm
Plasmid#170921PurposeExpresses spCas9 and sgRNA targeting the N-terminus of Ago1 for N-terminal taggingDepositorAvailable SinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRB131
Plasmid#180174PurposepAAV construct GluN1A714L (shRNA resistant)DepositorAvailable SinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pStr-KDEL_EPV-CXCL9-mCherry-SBP
Plasmid#170678PurposeExpresses ER-targeted streptavidin-KDEL (hook) and CXCL9-mCherry-SBP targeted to the ER lumen with an N-terminal EPV tripeptide (cargo) for RUSH.DepositorInsertsTagsSBP and mCherryExpressionMammalianMutationChanged threonine 23 to glutamate (T23E)PromoterIRES and T7Available SinceJan. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
SIN40C.SFFV.GFP.miR-ctrl
Plasmid#169305PurposeConstitutive overexpression of miR-ctrl with GFP as a reporter fluorescent proteinDepositorInsertmiR-ctrl
UseLentiviralTagsEGFPPromoterSFFVAvailable SinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
R9-FS1
Plasmid#125208PurposeExpressing human Rad9b frameshift mutation found in a neural tube defect patientDepositorAvailable SinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
R9-FS2
Plasmid#125209PurposeExpressing human Rad9b frameshift mutation found in a neural tube defect patientDepositorAvailable SinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
prCOrev
Plasmid#153961PurposeUsed to detect crossover-type DNA recombination events elicited by iso-positional nicking of two homologous DNA fragments (recombination analogous to Holliday's model)DepositorInsert5' and 3' EGFP fragments sharing a 386-bp sequence, separated by a NeoR gene cassette and arranged in the opposite direction
UseRetroviral; A reporter plasmid detecting dna reco…TagsNLS-ZFExpressionMammalianMutationNo mutationPromoterLTRAvailable SinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
prCO
Plasmid#153960PurposeUsed to detect crossover-type DNA recombination events elicited by iso-positional nicking of two homologous DNA fragments (recombination analogous to Holliday's model)DepositorInsert5' and 3' EGFP fragments sharing a 386-bp sequence, separated by a NeoR gene cassette and arranged in the same direction
UseRetroviral; A reporter plasmid detecting dna reco…TagsNLS-ZFExpressionMammalianMutationNo mutationPromoterLTRAvailable SinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONOR-SNCAe2-WT
Plasmid#85845PurposeDonor plasmid for SNCA exon2 wild type sequence. Also contains TagBFP and EGFPDepositorAvailable SinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
ORAI1-YFP V107M-L273D-L276D
Plasmid#114183PurposeORAI1 channel with C-terminal YFP, carrying the constitutively activating mutation V107M from TAM, and the mutations L273D-L276D leading to a deficiency in STIM1-mediated gating.DepositorInsertORAI1-YFP V107M-L273D-L276D (ORAI1 Human)
TagsYFPExpressionMammalianMutationV107M/L273D/L276DAvailable SinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
ORAI1-YFP T184M-L273D-L276D
Plasmid#114184PurposeORAI1 channel with C-terminal YFP, carrying the activating mutation T184M from TAM, and the mutations L273D-L276D leading to a deficiency in STIM1-mediated gating.DepositorInsertORAI1-YFP T184M-L273D-L276D (ORAI1 Human)
TagsYFPExpressionMammalianMutationT184M/L273D/L276DAvailable SinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONOR-SNCAe3-A53T
Plasmid#85848PurposeDonor plasmid for SNCA exon3 A53T sequence. Also contains EGFP and tagBFPDepositorInsertSNCA exon 3 homology arms (SNCA Human)
ExpressionBacterial and MammalianAvailable SinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
AL62_pDonor.DMD.TS
Plasmid#100287PurposeDMD intron 43-targeting plasmidDepositorInsertEGFP expression unit (DMD Synthetic, Human)
UseCRISPR and Synthetic Biology; Human targetingExpressionMammalianAvailable SinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSR13
Plasmid#69155Purposeread-outloxN mCherry to GFP switch, with swsn-1 promoter, gene (partially cDNA) and UTR, for integration on on ttTi5605, Mos Chr IIDepositorInsertsUseCre/Lox; MossciExpressionWormMutationcodon-optimzed index 1.0, codon-optimzed index 1.…Promoterrps-27 and swsn-1Available SinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
NKP91 GFP (1034)
Plasmid#62023Purposemammalian expression of nuclear envelope transmembrane proteinDepositorAvailable SinceJan. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEMS1156
Plasmid#29071PurposeExpression of the eGFP reporter gene was not detected in the brain and eye.DepositorAvailable SinceJan. 25, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEMS1387
Plasmid#29186PurposeExpression of the eGFP reporter gene was not detected in the brain and eye.DepositorAvailable SinceNov. 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEMS1216
Plasmid#29110PurposeExpression of the eGFP reporter gene was not detected in the brain and eye.DepositorAvailable SinceOct. 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEMS1432
Plasmid#29227PurposeExpression of the reporter gene was not detected in the brain and eye using eGFP/cre.DepositorAvailable SinceNov. 28, 2011AvailabilityAcademic Institutions and Nonprofits only -
C1m
Plasmid#120955PurposeMoClo golden gate assembly CD part for deGFP (deGFP3 with 4aa N-truncation, 9aa C-truncation, F64L, and C203T (numbered with original sequence); see https://www.fpbase.org/protein/degfp3/)DepositorInsertdeGFP
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only