We narrowed to 10,848 results for: ENA

1. R0010 (pLacI)_AB

   Plasmid

   #66004

   Purpose

   MoClo Basic Part: Controllable promoter - pLacI - lacI regulated (repressed by LacI+CAP. LacI, C0012, inhibited by IPTG) [A:R0010:B]

   Depositor

   Douglas Densmore

   Article

   Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

   Insert

   Controllable promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. mVenus-NES-SspB-MCS-P2A-iLIDcaax

   Plasmid

   #173864

   Purpose

   Mammalian expression of mVenus-NES-SspB-P2A-iLIDcaax (opto-control) containing multiple cloning sites (MCS)

   Depositor

   Takao Nakata

   Article

   Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

   Insert

   mVenus-NES-SspB-MCS-P2A-iLIDcaax
   

   Use

   Tags

   C-terminal CAAX-box and mVenus

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOPINK-OTUB2 (full-length, aa 1-234)

   Plasmid

   #61421

   Purpose

   Expresses human OTUB2 (full-length) in E. coli.

   Depositor

   David Komander

   Article

   Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

   Insert

   OTUB2 (Ubiquitin thioesterase OTUB2) (OTUB2 Human)
   

   Use

   Tags

   His6-GST-3C

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDIRECT_26G

   Plasmid

   #91149

   Purpose

   Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA , Plant Selection: PvUbi2:bar

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Engineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   D10A, H840A

   Promoter

   Available since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRSET-pr-mEos4b (mEosFP4b-V69T)

   Plasmid

   #99226

   Purpose

   Bacterial expression of the primed conversion capable protein pr-mEos4b (mEos4b-V69T).

   Depositor

   Periklis Pantazis

   Article

   Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

   Insert

   pr-mEos4b
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   V69T for primed conversion applications

   Promoter

   T7

   Available since

   Oct. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPPC001

   Plasmid

   #171138

   Purpose

   For integration of Sp.pCas9-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) with miniTn7T method

   Depositor

   Jesse Zalatan

   Article

   Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

   Insert

   Sp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   SoxS has R93A and S101A mutations

   Promoter

   Sp.pCas9, BBa_J23107

   Available since

   Oct. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. G1397 DddAtox-N–SaKKH-Cas9(D10A)

   Plasmid

   #157839

   Purpose

   expresses split DddAtox-Cas9 construct in mammalian cells

   Depositor

   David Liu

   Article

   Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

   Insert

   G1397 DddAtox-N–SaKKH-Cas9(D10A)–UGI–SV40 NLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. DCT.VV.Orf42

   Plasmid

   #12730

   Purpose

   This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

   Depositor

   Jack Bennink, Jon Yewdell

   Article

   Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

   Insert

   VV.Orf42
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 1, 2006

   Availability

   Academic Institutions and Nonprofits only

9. pGEXNKI-GST-3C-LIC

   Plasmid

   #108710

   Purpose

   Expression of a GST-tagged target protein with 3C protease cleavage site.

   Depositor

   Titia Sixma

   Article

   Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

   Type

   Empty backbone

   Use

   Tags

   GST-3C protease cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Aug. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPKm-196

    Plasmid

    #90511

    Purpose

    pcDNA3 - CMVmin PIF6-DBD, expressing PIF6-DBD under CMV promoter

    Depositor

    Todd Coleman

    Article

    Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

    Insert

    PIF6-DBD
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDIRECT_25G

    Plasmid

    #91144

    Purpose

    Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA , Plant Selection: PvUbi2:hpt II

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Engineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D10A, H840A

    Promoter

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. DCT.VV.Orf117

    Plasmid

    #12805

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jack Bennink, Jon Yewdell

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf117
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2006

    Availability

    Academic Institutions and Nonprofits only

13. pDIRECT_23D

    Plasmid

    #91141

    Purpose

    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers, Plant Selection: 2x35S:bar

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D10A, H840A

    Promoter

    Available since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSLiP-G2-RacE

    Plasmid

    #188966

    Purpose

    L-Rhamnose inducible catalytically inactive Cas9 with sgRNA and racE gene for bacterial gene knockdown

    Depositor

    Seung-Goo Lee

    Article

    Kim et al Nucleic Acids Res. 2022 Dec 9;50(22):131

    Inserts

    dCas9
    sgRNA: gttagacgctgattacatggactagg
    racE
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PrhaBAD

    Available since

    Sept. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYPQ292 (AaCas12b)-D570A-TV-MS2-TV

    Plasmid

    #136381

    Purpose

    CRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) fused with TV activation system, followed by T2A and MCP-TV fusion protein

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Insert

    AaCas12b (D570A)-TV-T2A-MCP-TV
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D570A

    Promoter

    Available since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRvCAHS1-mEGFP-NLS

    Plasmid

    #205013

    Purpose

    The vector contains 1kbp of the upstream and downstream regions of the cahs1 gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between.

    Depositor

    Kazuharu Arakawa

    Article

    Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12

    Insert

    mEGFP-NLS flanked by 1kbp upstream and downstream of the cahs1 gene from Ramazzottius varieornatus
    

    Use

    Tardigrade expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRvSAHS1-mCherry-NLS

    Plasmid

    #205009

    Purpose

    The vector contains 1kbp of the upstream and downstream regions of the sahs1 gene from Ramazzottius varieornatus, with the mCherry gene with NLS sequence positioned in between.

    Depositor

    Kazuharu Arakawa

    Article

    Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12

    Insert

    mCherry-NLS flanked by 1kbp upstream and downstream of the sahs1 gene from R. varieornatus
    

    Use

    Tardigrade expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. LentiCRISPR v2-sgAMPK alpha1 clone2

    Plasmid

    #162120

    Purpose

    Lentiviral sgRNA plasmid targeting human AMPK alpha1

    Depositor

    Boyi Gan

    Article

    Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

    Insert

    sgAMPK alpha1 (PRKAA1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. DCT.VV.Orf196

    Plasmid

    #12884

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jack Bennink, Jon Yewdell

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf196
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

20. SpyCas9 PE2-3HA

    Plasmid

    #169853

    Purpose

    Mammalian Expression, SpyCas9 prime editor with 3HA-tag

    Depositor

    Scot Wolfe

    Article

    Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

    Insert

    SpCas9-H840A-M-MLV-3HA
    

    Use

    Tags

    3XHA tag and bpSV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available since

    June 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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