We narrowed to 4,634 results for: HRE

1. pJC20cdc8.A18T

   Plasmid

   #99360

   Purpose

   Bacterial expression vector containing cDNA encoding for the fission yeast tropomyosin, Cdc8 with the amino acid substitution Ala-18-Thr.

   Depositor

   Dan Mulvihill

   Article

   Johnson et al Biochem Biophys Res Commun. 2017 Oct 25.

   Insert

   cdc8 (cdc8 Fission Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Alanine 18 to Threonine

   Promoter

   T7

   Available Since

   Oct. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJC20cdc8.110

   Plasmid

   #99363

   Purpose

   Bacterial expression vector containing cDNA encoding for the temperature sensitive fission yeast tropomyosin mutant, Cdc8.110.

   Depositor

   Dan Mulvihill

   Article

   Johnson et al Biochem Biophys Res Commun. 2017 Oct 25.

   Insert

   cdc8 (cdc8 Fission Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Alanine 18 to Threonine and Glutamic acid 31 to L…

   Promoter

   T7

   Available Since

   Oct. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTH731-2µ-RLuc/minCFLuc

   Plasmid

   #40601

   Depositor

   Tobias von der Haar

   Article

   Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

   Inserts

   Firefly Luciferase
   Renilla luciferase
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   All codons of the original FLuc gene were exchang…

   Promoter

   ADH1 and TDH3

   Available Since

   Oct. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGL4-AARE-luc2P-Hygro

   Plasmid

   #101787

   Purpose

   Luferase reporter plasmid containing three tandem repeats of the amino acid response element (AARE)

   Depositor

   Seiichi Oyadomari

   Article

   Miyake et al FASEB J. 2016 Feb;30(2):798-812. doi: 10

   Insert

   3xAARE
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   minimal TATA-box promoter with low basal activity

   Available Since

   Sept. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-Tet-MKK6(DD)-Puro

   Plasmid

   #86094

   Purpose

   Tet/Dox inducible (TetR) constitutively active mutant MKK6/MAP2K6 in lentiviral vector

   Depositor

   Cindy Miranti

   Article

   Frank et al J Cell Sci. 2017 Apr 26. pii: jcs.197152

   Insert

   MKK6 (MAP2K6 Human)
   

   Use

   Lentiviral

   Tags

   Myc tag

   Expression

   Mammalian

   Mutation

   Serine 207 and Threonine 211 both changed to Aspa…

   Promoter

   CMV/TetO

   Available Since

   Nov. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA-EGFP MASTL G44S (siRNA resistant)

   Plasmid

   #191012

   Purpose

   Expresses EGFP-tagged kinase-dead MASTL G44S with resistance to MASTL siRNA (ACGCCTTATTCTAGCAAATTA)

   Depositor

   Johanna Ivaska

   Article

   Taskinen et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   Microtubule-associated serine/threonine kinase like (MASTL Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   G44S

   Promoter

   Available Since

   Nov. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMG071 MBP-GSK3β_S9A-HA-His, GST_λPPase

   Plasmid

   #196185

   Purpose

   Co-expresses MBP-GSK3β_S9A-HA-His (human GSK3β with S9A mutation as a fusion protein with MBP, HA, and His-tags) and GST_λPPase in E.coli to produce unphosphorylated MBP-GSK3β_S9A-HA-His

   Depositor

   Jesse Zalatan

   Article

   Gavagan et al Elife. 2023 Aug 7;12:e85444. doi: 10.755

   Inserts

   GSK3B (GSK3B Human)
   λPPase
   

   Use

   Tags

   GST, HA, His6, and MBP

   Expression

   Bacterial

   Mutation

   changed Serine 9 to Alanine

   Promoter

   Tac

   Available Since

   Jan. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBridge-tyrosinase.σ1A

   Plasmid

   #197381

   Purpose

   Expression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-1 σ1A fusion protein in yeast (yeast three-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.

   Inserts

   tyrosinase cytosolic tail
   AP-1 σ1A
   

   Use

   Tags

   GAL4-DNA binding domain fragment, HA tag, and SV4…

   Expression

   Yeast

   Mutation

   Contains an extra 42 nucleotides encoding 14 resi…

   Promoter

   ADH1 and MET25

   Available Since

   March 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTH734-2µ-RLuc/stopCFLuc

   Plasmid

   #40609

   Depositor

   Tobias von der Haar

   Article

   Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

   Inserts

   Firefly Luciferase (nonsense mutant)
   Renilla luciferase
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   All codons of the original FLuc gene were exchang…

   Promoter

   ADH1 and TDH3

   Available Since

   Oct. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. Flag-KLF6-4D (1054)

    Plasmid

    #49490

    Purpose

    expresses human Flag tagged KLF6 with 4D mutation

    Depositor

    Scott Friedman

    Article

    Lang et al Oncogene. 2012 Oct 22. doi: 10.1038/onc.

    Insert

    KFL6-4D (KLF6 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    three serines and one tyrosine mutated to aspart…

    Promoter

    CMV

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

11. RKF1 X030_pECIA14

    Plasmid

    #114856

    Purpose

    Prey vector RKF1 X030_pECIA14 should be used with bait vector RKF1 X030_pECIA2.

    Depositor

    Youssef Belkhadir

    Article

    Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

    Insert

    AT1G29750 (RKF1 Mustard Weed)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Metallothionein (Copper-Inducible)

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGL4.23 A82V/T230A/D637G 2014 EBOV Delta-Mucin-Like-Domain

    Plasmid

    #86026

    Purpose

    Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression vector

    Depositor

    Jeremy Luban, Pardis Sabeti

    Article

    Diehl et al Cell. 2016 Nov 3;167(4):1088-1098.e6. do

    Insert

    Makona Ebola Virus Glycoprotein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Lacks mucin-like domain; Changed alanine 82 to va…

    Promoter

    CMV IE

    Available Since

    July 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTH752-CEN-RLuc/min103maxCFLuc

    Plasmid

    #38218

    Depositor

    Tobias von der Haar

    Article

    Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

    Inserts

    Firefly Luciferase
    Renilla luciferase
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    The first 103 codons of the original FLuc gene we…

    Promoter

    ADH1 and TDH3 (=GPD)

    Available Since

    Oct. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTH751-CEN-RLuc/min53maxCFLuc

    Plasmid

    #38217

    Depositor

    Tobias von der Haar

    Article

    Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

    Inserts

    Firefly Luciferase
    Renilla luciferase
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    The first 53 codons of the original FLuc gene wer…

    Promoter

    ADH1 and TDH3 (=GPD)

    Available Since

    Oct. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. psiCHECK-1-BRAF_WT&Mut-SM

    Plasmid

    #215916

    Purpose

    This plasmid encodes luciferase which has siRNA binding sites in 3'UTR for detecting the siRNA silencing activity.

    Depositor

    Kumiko Ui-Tei

    Article

    Song et al Cancers (Basel). 2022 Jun 28;14(13):3162

    Insert

    three tandem repeats of seed-matched (SM) sequence of siBRAF_WT&Mut (BRAF Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. psiCHECK-1-BRAF_WT-SM

    Plasmid

    #215917

    Purpose

    This plasmid encodes luciferase which has siRNA binding sites in 3'UTR for detecting the siRNA silencing activity.

    Depositor

    Kumiko Ui-Tei

    Article

    Song et al Cancers (Basel). 2022 Jun 28;14(13):3162

    Insert

    three tandem repeats of seed-matched (SM) sequence of siBRAF_ WT (BRAF Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. psiCHECK-1-BRAF_Mut1-SM

    Plasmid

    #215918

    Purpose

    This plasmid encodes luciferase which has siRNA binding sites in 3'UTR for detecting the siRNA silencing activity.

    Depositor

    Kumiko Ui-Tei

    Article

    Song et al Cancers (Basel). 2022 Jun 28;14(13):3162

    Insert

    three tandem repeats of seed-matched (SM) sequence of siBRAF_ Mut1 (BRAF Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    BRAF

    Promoter

    Available Since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. psiCHECK-1-BRAF_Mut2-SM

    Plasmid

    #215919

    Purpose

    This plasmid encodes luciferase which has siRNA binding sites in 3'UTR for detecting the siRNA silencing activity.

    Depositor

    Kumiko Ui-Tei

    Article

    Song et al Cancers (Basel). 2022 Jun 28;14(13):3162

    Insert

    three tandem repeats of seed-matched (SM) sequence of siBRAF_ Mut2 (BRAF Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    BRAF

    Promoter

    Available Since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AAV-TRE-dSaCas9-NLS-3xHA-3xSunTag_bGHpA

    Plasmid

    #177344

    Purpose

    AAV expression of a catalytically inactive SaCas9 (dSaCas9) fused with three copies of SunTag sequence from doxycycine-inducible promoter

    Depositor

    Naoki Yamamoto

    Article

    Yamamoto et al Research Square [Preprint] 2021. doi: 10

    Insert

    dead hSaCas9
    

    Use

    AAV and CRISPR

    Tags

    3x GCN4 peptide, 3xHA, and NLS

    Expression

    Mammalian

    Mutation

    D10A, N580A

    Promoter

    Tetracycline-dependent promoters

    Available Since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. CamKII-LGI1-T380A-pHluorin

    Plasmid

    #185543

    Purpose

    Expresses mutant T380A LGI1-pHluorin under the CamKII promoter

    Depositor

    Jaime de Juan-Sanz

    Article

    Cuhadar et al Cell Rep. 2024 May 28;43(5):114186. doi:

    Insert

    LGI1 (Lgi1 Rat)
    

    Use

    Lentiviral

    Tags

    pHluorin

    Expression

    Mammalian

    Mutation

    changed threonine 380 for alanine

    Promoter

    CamKII

    Available Since

    May 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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