We narrowed to 9,202 results for: sacs

1. pRS406 ATG1-ATG7-3'UTR

   Plasmid

   #166847

   Purpose

   Expresses Atg1 with ATG7 3'UTR in yeasts.

   Depositor

   Daniel Klionsky

   Article

   Gatica et al Mol Cell. 2019 Jan 17;73(2):314-324.e4.

   Insert

   ATG1 (ATG1 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ATG1 Promoter

   Available since

   April 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRS405 VAC8-G2A,C4A,C5A,C7A-RFP

   Plasmid

   #166842

   Purpose

   Expresses a myristoylation and palmitoylation mutant Vac8-RFP in yeasts, mutations are Gly2Ala, Cys4Ala, Cys5Ala, Cys7Ala

   Depositor

   Daniel Klionsky

   Article

   Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

   Insert

   VAC8 (VAC8 Budding Yeast)
   

   Use

   Tags

   RFP

   Expression

   Yeast

   Mutation

   G2A, C4A, C5A, C7A

   Promoter

   VAC8 promoter

   Available since

   April 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRS405 VAC8-G2A,C4A,C5A,C7A-GFP

   Plasmid

   #166839

   Purpose

   Expresses a myristoylation and palmitoylation mutant Vac8-GFP in yeasts, mutations are Gly2Ala, Cys4Ala, Cys5Ala, Cys7Ala

   Depositor

   Daniel Klionsky

   Article

   Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

   Insert

   VAC8 (VAC8 Budding Yeast)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   G2A, C4A, C5A, C7A

   Promoter

   VAC8 promoter

   Available since

   April 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRS405 VAC8-C4A,C5A,C7A-GFP

   Plasmid

   #166838

   Purpose

   Expresses a palmitoylation mutant Vac8-GFP in yeasts, mutations are Cys4Ala, Cys5Ala, Cys7Ala

   Depositor

   Daniel Klionsky

   Article

   Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

   Insert

   VAC8 (VAC8 Budding Yeast)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   C4A, C5A, C7A

   Promoter

   VAC8 promoter

   Available since

   April 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNTI751 pPGK1-GCN4(UTR)-ZEM(TF)

   Plasmid

   #164916

   Purpose

   Expresses the ZEM transcription factor under the translational control of the yeast GCN4 5'UTR

   Depositor

   Nicholas Ingolia

   Article

   Muller et al Science. 2020 Dec 11;370(6522). pii: 370

   Insert

   GCN4(UTR)-ZEM(TF)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   PGK1 (yeast)

   Available since

   Feb. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. CTMP

   Plasmid

   #129560

   Purpose

   cytoplasmic marker

   Depositor

   Inaki Ruiz-Trillo

   Article

   Kozyczkowska et al Curr Biol. 2021 Sep 27;31(18):4104-4110.

   Insert

   mCherry
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX-2T-RNAPII-A2-14-HA

   Plasmid

   #160689

   Purpose

   Expression of GST-RNA Polymerase II C-terminal domain fusion protein. Contains an S-to-A mutation in position 2 of 14 of the consensus heptapeptide repeats shown to be phosphorylated by Cdc2 kinase.

   Depositor

   Jeffry Corden

   Article

   Patturajan et al J Biol Chem. 1998 Feb 20;273(8):4689-94.

   Insert

   RNA polymerase II CTD
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Contains an S-to-A mutation in position 2 of 14 o…

   Promoter

   Available since

   Nov. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMJB1-RPL1B-Cl

   Plasmid

   #160430

   Purpose

   Expresses "cre-less" yeast RPL1B tagged with mCherry under the GAL1 promoter

   Depositor

   Gary Stormo

   Article

   Roy et al Commun Biol. 2020 Dec 11;3(1):761. doi:

   Insert

   RPL1B
   

   Use

   Tags

   mCherry

   Expression

   Yeast

   Mutation

   Removed introns, shuffled codons to vary nucleoti…

   Promoter

   S. cerevisiae GAL1

   Available since

   Nov. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-Gal4-RNF169UBD

   Plasmid

   #119013

   Purpose

   Mammalian expression of fusion protein of yeast Gal4 with human RNF169 Ubiquitin binding domain and BFP expression

   Depositor

   Ralf Kuehn

   Article

   Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

   Insert

   Gal4 (GAL4 Budding Yeast)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDUAL-Pnmt1-Ost4-GFP

    Plasmid

    #154368

    Purpose

    Yeast expression of Ost4

    Depositor

    Wanzhong He

    Article

    Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

    Insert

    Ost4 (ost4 Fission Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. p415 Glo3 R59K Δ2x+ve

    Plasmid

    #112655

    Purpose

    Expression S.cerevisiae

    Depositor

    Blanche Schwappach

    Article

    Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

    Insert

    Glo3 R59K Δ2x+ve
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    R59K, mutations (R223E, K224E, K225E, K233E, K234…

    Promoter

    Met25

    Available since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. p415 GFP-Glo3 Δ2x+ve

    Plasmid

    #112662

    Purpose

    Expression S.cerevisiae

    Depositor

    Blanche Schwappach

    Article

    Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

    Insert

    GFP- Glo3 Δ2x+ve
    

    Use

    Tags

    sfGFP

    Expression

    Yeast

    Mutation

    Mutations (R223E, K224E, K225E, K233E, K234E, K23…

    Promoter

    Met25

    Available since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pYPS1-Venus

    Plasmid

    #133118

    Purpose

    Plasmid contains a reporter gene for Crz1. The 1000bp promoter of the gene pYPS1 driving a Venus.

    Depositor

    Hana El-Samad

    Article

    Chen et al Cell Syst. 2020 Sep 4. pii: S2405-4712(2

    Insert

    pYPS1-YFP
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCMK2-Venus

    Plasmid

    #133119

    Purpose

    Plasmid contains a reporter gene for Crz1. The 1000bp promoter of the gene pCMK2 driving a Venus.

    Depositor

    Hana El-Samad

    Article

    Chen et al Cell Syst. 2020 Sep 4. pii: S2405-4712(2

    Insert

    pCMK2-YFP
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGYP7-Venus

    Plasmid

    #133120

    Purpose

    Plasmid contains a reporter gene for Crz1. The 1000bp promoter of the gene pGYP7 driving a Venus.

    Depositor

    Hana El-Samad

    Article

    Chen et al Cell Syst. 2020 Sep 4. pii: S2405-4712(2

    Insert

    pGYP7-YFP
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. YCplac33-NAT_NDT1

    Plasmid

    #140593

    Purpose

    Express NDT1 in S. cerevisiae

    Depositor

    David Sabatini

    Article

    Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

    Insert

    NDT1 with 5' and 3' UTRs (YIA6 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    NDT1 promoter and 3'UTR

    Available since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRS314-U6-G96C/A97C/U100C/U101C

    Plasmid

    #82328

    Purpose

    U6 telestem mutant (G96C/A97C/U100C/U101C) for in vivo studies

    Depositor

    Aaron Hoskins

    Article

    Rodgers et al Nucleic Acids Res. 2016 Dec 15;44(22):10

    Insert

    U6 RNA with G96C/A97C/U100C/U101C mutation (snR6 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    U6 telestem mutant (A34G/A35G/G96C/A97C/U100C/U10…

    Promoter

    Available since

    Sept. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRS314-U6-A34G/A35G/G96C/A97C

    Plasmid

    #81238

    Purpose

    U6 telestem mutant (A34G/A35G/G96C/A97C) for in vivo studies

    Depositor

    Aaron Hoskins

    Article

    Rodgers et al Nucleic Acids Res. 2016 Dec 15;44(22):10

    Insert

    U6 RNA with A34G/A35G/G96C/A97C mutation (snR6 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    U6 telestem mutant (A34G/A35G/G96C/A97C)

    Promoter

    Available since

    Sept. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDUAL-P41nmt1-Sad1-GFP

    Plasmid

    #154366

    Purpose

    Yeast expression of Sad1

    Depositor

    Wanzhong He

    Article

    Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

    Insert

    Sad1 (sad1 Fission Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDUAL-P41nmt1-Nup124-GFP

    Plasmid

    #154364

    Purpose

    Yeast expression of Nup124

    Depositor

    Wanzhong He

    Article

    Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

    Insert

    Nup124 (nup124 Fission Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pDUAL-Pnmt1-Ost4-GFP-MTalpha

    Plasmid

    #154370

    Purpose

    Yeast expression of Ost4

    Depositor

    Wanzhong He

    Article

    Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

    Insert

    Ost4 (ost4 Fission Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pDUAL-P41nmt1-Sad1-GFP-MTn

    Plasmid

    #154367

    Purpose

    Yeast expression of Sad1

    Depositor

    Wanzhong He

    Article

    Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

    Insert

    Sad1 (sad1 Fission Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pGI3EM29

    Plasmid

    #135489

    Purpose

    Agrobacterium T-DNA with Spun H2Bpr driving hph-mCitrine

    Depositor

    Nicolas Buchler

    Article

    Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

    Insert

    hph SpunH2A/Bpr hph-mCitrine
    

    Use

    Tags

    mCitrine

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    SpunH2A/Bpr (divergent)

    Available since

    May 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGI3EM30

    Plasmid

    #135490

    Purpose

    Agrobacterium T-DNA with Spun H2Bpr driving hph-mClover3

    Depositor

    Nicolas Buchler

    Article

    Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

    Insert

    hph SpunH2A/Bpr hph-mClover3
    

    Use

    Tags

    mClover3

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    SpunH2A/Bpr (divergent)

    Available since

    May 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pGI3EM31

    Plasmid

    #135491

    Purpose

    Agrobacterium T-DNA with Spun H2Bpr driving hph-mCerulean3

    Depositor

    Nicolas Buchler

    Article

    Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

    Insert

    hph SpunH2A/Bpr hph-mCerulean3
    

    Use

    Tags

    mCerulean3

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    SpunH2A/Bpr (divergent)

    Available since

    May 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pEPOR1CB0015

    Plasmid

    #117551

    Purpose

    Level 1 Golden Gate Cassette: SaCas9 expression cassette for dicotyledonous plants

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    2x35S+5'UTR OMEGA (pICH51288) + SaCas9 (no stop codon) (pEPOR0SP0012) + YFP (pICSL50005) + Nos (pICH41421)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    2x35S+5'UTR OMEGA (pICH51288)

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pSTAP-seq_fly-4xUAS

    Plasmid

    #125149

    Purpose

    Vector to measure the activity of CP candidates in response to a tethered (4xUAS) GAL4-DBD-COF by determining the abundance of transcripts originating from each candidate in fly cells

    Depositor

    Alexander Stark

    Article

    Haberle et al Nature. 2019 Jun;570(7759):122-126. doi:

    Insert

    4 x upstream activating sequence (UAS)
    

    Use

    Stap-seq screening vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pRS-ATG1-ATG7 3'UTR(406)

    Plasmid

    #122070

    Purpose

    Expressing ATG1 with ATG7 3'UTR

    Depositor

    Daniel Klionsky

    Article

    Liu et al PLoS Biol. 2019 Apr 11;17(4):e3000219. d

    Insert

    serine/threonine protein kinase ATG1 (ATG1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    The native 3'UTR was replaced by ATG7 3'…

    Promoter

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pGBDU-Atg17(F317D, I325A)

    Plasmid

    #122275

    Purpose

    For Yeast-two-hybrid analysis

    Depositor

    Daniel Klionsky

    Article

    Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

    Insert

    ATG17
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Phenylalanine 317 to Aspartic acid; Isoleucine 32…

    Promoter

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pEPOR2KN0078

    Plasmid

    #117645

    Purpose

    Level2 MoClo construct, containing level1 plant expression cassettes for eSaCas9(pEPOR1CB0113), sgRNA{AT1G73460}(pEPOR1CB0099) and sgRNA{AT1G73460}(pEPOR1CB0110) to Protein kinase superfamily protein.

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    [35S:eSaCas9(pEPOR1CB0113) ] +[AtU6-26:sgRNA]
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge