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pET28a-TS2126 RNA Ligase

Plasmid

#119941

Purpose

Express TS2126 RNA ligase in E. coli

Depositor

Takashi Ito

Article

Miura et al Nucleic Acids Res. 2019 May 22. pii: 549

Insert

TS2126 RNA ligase

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 23, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a-mOct4 Full length(FL)

Plasmid

#206394

Purpose

Expresses Oct4 with a 6xHis tag for protein purification

Depositor

Ralf Jauch

Insert

Oct4 (Pou5f1 Mouse)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a-2xMreBN-mCherry-His

Plasmid

#133626

Purpose

Expression vector for mCherry fused to a tandem repeat of the E. coli MreB membrane targeting sequence (MTS) (N-terminal fusion) and a C-terminal His-tag for purification.

Depositor

Petra Schwille

Article

Ramm et al Nat Commun. 2018 Sep 26;9(1):3942. doi:

Insert

2xMreB-mCherry-His

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-SENP7 (catalytic domain)

Plasmid

#16360

Depositor

Guy Salvesen

Article

Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

Insert

human SENP7 catalytic domain (SENP7 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Amino Acid 640 to 984 catalytic domain of human S…

Promoter

Available since

Dec. 19, 2007

Availability

Academic Institutions and Nonprofits only
pET28a-6XHis-SUMO-SIRT2

Plasmid

#102622

Purpose

Expresses a truncation (aa38-356) of human SIRT2 isoform 1 in bacteria for purification

Depositor

Hening Lin

Article

Jing et al Cancer Cell. 2016 Mar 14;29(3):297-310.

Insert

SIRT2 (SIRT2 Human)

Use

Tags

6XHis-SUMO

Expression

Bacterial

Mutation

Deleted amino acids 1-37 and 357-389

Promoter

Available since

Feb. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-SENP2 (catalytic domain)

Plasmid

#16357

Depositor

Guy Salvesen

Article

Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

Insert

human SENP2 catalytic domain (SENP2 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Amino Acid 365 to 590 catalytic domain of human S…

Promoter

Available since

Dec. 19, 2007

Availability

Academic Institutions and Nonprofits only

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Pet28a-His-tag-BS2

Plasmid

#220347

Purpose

E. coli expression vector to purify BS2

Depositor

Bryan Dickinson

Article

Pani et al Nat Chem. 2024 Apr 9. doi: 10.1038/s4155

Insert

BS2

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

Available since

June 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pET28a-SENP1 (catalytic domain)

Plasmid

#16356

Depositor

Guy Salvesen

Article

Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

Insert

human SENP1 catalytic domain (SENP1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Amino Acid 419 to 644 catalytic domain of human S…

Promoter

Available since

Dec. 19, 2007

Availability

Academic Institutions and Nonprofits only

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pet28a-ClbB-NRPS-NHis

Plasmid

#49217

Purpose

NRPS module of ClbB containing CAT domains with N-terminal 6His tag

Depositor

Emily Balskus

Article

Brotherton et al J Am Chem Soc. 2013 Mar 6;135(9):3359-62

Insert

ClbB-NRPS

Use

Tags

6x His tag and T7 tag

Expression

Bacterial

Mutation

contains aa4-1080 of reference sequence NP_754362…

Promoter

T7

Available since

March 26, 2014

Availability

Academic Institutions and Nonprofits only

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pET28a(+) NRP1 b1 (273-427)

Plasmid

#162734

Purpose

Bacterial expression of the b1 domain from the human Neuropilin 1 receptor (NRP1). With an N-terminal 6His tag and thrombin cleavage site.

Depositor

Brett Collins

Article

Daly et al Science. 2020 Oct 20. pii: science.abd30

Insert

Human NRP1 b1 domain residues 273-427

Use

Tags

Expression

Bacterial

Mutation

Codon optimised

Promoter

Available since

April 27, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pET28a-His6-ODC-Ctag

Plasmid

#163614

Purpose

Expresses truncated human Ornithine Decarboxylase in bacterial cells for binding to Ornithine Decarboxylase Antizyme.

Depositor

Mark Howarth

Article

Scheu et al Nat Commun. 2021 Jan 29;12(1):717. doi:

Insert

ODC (ODC1 Human)

Use

Tags

C-tag and His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pF709-pET28a-Hs-FL-ETFbeta-NHis wt

Plasmid

#85110

Purpose

expression of recombinant human full-length ETFbeta wt with N-terminal 6xHis-tag

Depositor

Pal Falnes

Article

Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

Insert

human full-length ETFbeta (ETFB Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

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His6-SUMO-pET28a-EfaCas1

Plasmid

#112793

Purpose

To express enterococcus faecalis Cas1 protein in E. coli

Depositor

Ailong Ke

Article

Xiao et al Nature. 2017 Oct 5;550(7674):137-141. do

Insert

EfaCas1

Use

Tags

His6-SUMO

Expression

Bacterial

Mutation

Promoter

Available since

July 20, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a(+)-SpdCas9-SBiT_Strep-Tag-II

Plasmid

#198750

Purpose

Expresses S. Pyogenes dCas9 fused to SmallBiT fragment of split NanoLuc luciferase

Depositor

Maarten Merkx

Article

van der Veer et al ACS Cent Sci. 2023 Mar 15;9(4):657-667.

Insert

SpdCas9 - SmallBiT

Use

Tags

Strep-tag II

Expression

Bacterial

Mutation

D10A, H840A (nuclease inactivating mutations in &…

Promoter

T7

Available since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a-SARS-CoV-2 NSP13

Plasmid

#176492

Purpose

Expresses the N-terminal His6-tagged SARS-CoV2 helicase (NSP13) in E coli.

Depositor

Xiaodong Cheng

Article

White et al J Phys Chem Lett. 2020 Nov 5;11(21):9144

Insert

SARS-CoV-2 NSP13 (ORF1ab SARS-CoV)

Use

Tags

His6 and T7

Expression

Bacterial

Mutation

I570 from SARS-CoV NSP13 was mutated to V to conv…

Promoter

T7

Available since

Jan. 10, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pET28a(+)-SpdCas9-LBiT_Strep-Tag-II

Plasmid

#198751

Purpose

Expresses S. Pyogenes dCas9 fused to LargeBiT fragment of split NanoLuc luciferase

Depositor

Maarten Merkx

Article

van der Veer et al ACS Cent Sci. 2023 Mar 15;9(4):657-667.

Insert

SpdCas9 - LargeBiT

Use

Tags

Strep-tag II

Expression

Bacterial

Mutation

D10A, H840A (nuclease inactivating mutations in &…

Promoter

T7

Available since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a T7pCONS TIR-1 sfGFP

Plasmid

#154463

Purpose

pET28a with a design flaw in the T7 promoter fixed, and a synthetically evolved TIR. Contains the coding sequence for sfGFP

Depositor

Daniel Daley

Article

Shilling et al Commun Biol. 2020 May 7;3(1):214. doi: 1

Insert

super folder GFP

Use

Tags

His-thrombin

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a SUMO Nup53xl 1-320

Plasmid

#85998

Purpose

bacterial expression of his6 and SUMO tagged Xenopus laevis Nup53

Depositor

Wolfram Antonin

Article

Vollmer et al EMBO J. 2012 Oct 17;31(20):4072-84. doi:

Insert

Xenopus laevis Nup53 (nup35.L Frog)

Use

Tags

SUMO

Expression

Bacterial

Mutation

codon optimzed for bacterial expression

Promoter

T7

Available since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a(+) 6xHis-3xFlag-IFIT5

Plasmid

#53561

Purpose

bacterial expression of IFIT5

Depositor

Kathleen Collins

Article

Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

Insert

IFIT5 (IFIT5 Human)

Use

Tags

3xFLAG, 6xHIS, and TEV site

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 29, 2014

Availability

Academic Institutions and Nonprofits only
pET28a-SdrG_N2N3-HIS-ybbr

Plasmid

#101238

Purpose

SdrG N2 and N3 domains with C-terminal ybbr tag for covalent immobilization

Depositor

Hermann Gaub

Article

Milles et al Science. 2018 Mar 30;359(6383):1527-1533

Insert

SdrG_N2N3

Use

Tags

H3V 3C, HIS, and ybbr

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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