We narrowed to 10,794 results for: T7

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pMAT11

Plasmid

#112592

Purpose

Used for expression of protein in E. coli as Thrombin and TEV cleavableN-terminal 6His-tag-MBP fusions and without NcoI site within MBP.

Depositor

Marko Hyvönen, Johan Peränen

Article

Peranen et al Anal Biochem. 1996 May 1;236(2):371-3.

Type

Empty backbone

Tags

6His-MBP

Expression

Bacterial

Available Since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pAB167

Plasmid

#101672

Purpose

Opto-T7RNAP*(563), equal expr.

Depositor

Mustafa Khammash

Article

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

Opto-T7RNAP*(563), equal expr.

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pRSF-T20S

Plasmid

#110805

Purpose

expresses Thermoplasma acidophilium 20S proteasome in E. Coli

Depositor

Yifan Cheng

Article

Yu et al Nat Commun. 2020 Sep 11;11(1):4580. doi:

Inserts

PsmB
PsmA

Tags

tev-His

Expression

Bacterial

Mutation

N2D and Q2E

Promoter

T7 and T7 promoter

Available Since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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p5-39

Plasmid

#118088

Purpose

FKBP-TSGGSG-Split C-terminal T7 RNAP expression plasmid

Depositor

Bryan Dickinson

Article

Pu et al Nat Chem Biol. 2017 Apr;13(4):432-438. d

Insert

FKBP-TSGGSG-Split C-terminal T7 RNAP expression plasmid

Use

Synthetic Biology

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pKEW189-MBP-TEV-AcrVA1

Plasmid

#115660

Purpose

Expresses AcrVA1 in bacteria via T7 RNAP, for purification

Depositor

Jennifer Doudna

Article

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

AcrVA1

Tags

MBP-TEV

Expression

Bacterial

Promoter

T7 RNAP

Available Since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pTHSSd_34

Plasmid

#59960

Purpose

PTac GFP expression

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

Insert

sfGFP

Expression

Bacterial

Mutation

N/A

Promoter

PTac

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pAB152

Plasmid

#101663

Purpose

Opto-T7RNAP*(563-F2)

Depositor

Mustafa Khammash

Article

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

Opto-T7RNAP*(563-F2)

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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Fast-GCaMP6f-RS09

Plasmid

#67160

Purpose

genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

Depositor

Samuel Wang

Article

Badura et al Neurophotonics. 2014 Oct;1(2):025008.

Insert

Fast-GCaMP6f-RS09

Tags

6xHis, T7 tag, and Xpress tag

Expression

Bacterial

Mutation

L414T within GCaMP6f

Promoter

T7

Available Since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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pRSETb-Ferritin-Dronpa

Plasmid

#101194

Purpose

Expresses a Ferritin-Dronpa fusion construct in E. coli

Depositor

Peter Dedecker

Article

Duwe et al Chem Commun (Camb). 2017 Jun 29;53(53):7

Insert

Ferritin-Dronpa

Tags

6xHis, T7 tag, and Xpress tag

Expression

Bacterial

Promoter

T7

Available Since

Sept. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pKEW212-MBP-TEV-AcrVA4

Plasmid

#115658

Purpose

Expresses AcrVA4 in bacteria via T7 RNAP, for purification

Depositor

Jennifer Doudna

Article

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

AcrVA4

Tags

MBP-TEV

Expression

Bacterial

Promoter

T7 RNAP

Available Since

Oct. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pAB168

Plasmid

#101671

Purpose

Opto-T7RNAP*(563-F1), equal expr.

Depositor

Mustafa Khammash

Article

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

Opto-T7RNAP*(563-F1), equal expr.

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pAB203

Plasmid

#101675

Purpose

Opto-T7RNAP(563-F1)

Depositor

Mustafa Khammash

Article

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

Opto-T7RNAP(563-F1)

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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Fast-GCaMP6f-RS06

Plasmid

#67159

Purpose

genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

Depositor

Samuel Wang

Article

Badura et al Neurophotonics. 2014 Oct;1(2):025008.

Insert

Fast-GCaMP6f-RS06

Tags

6xHis, T7 tag, and Xpress tag

Expression

Bacterial

Mutation

M374Q within GCaMP6f

Promoter

T7

Available Since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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pAB164

Plasmid

#101667

Purpose

Opto-T7RNAP*(563), half expr.

Depositor

Mustafa Khammash

Article

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

Opto-T7RNAP*(563), half expr.

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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p7XC3GH

Plasmid

#47066

Purpose

FX cloning E.coli expression vector with T7 promoter and C-terminal 3C protease cleavage site, GFP and 10x His tags

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Tags

3C protease site (PreScission site), GFP, and dec…

Expression

Bacterial

Promoter

T7

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pRSETb-ffDronpa-F

Plasmid

#101193

Purpose

A fast switching Dronpa variant

Depositor

Peter Dedecker

Article

Duwe et al Chem Commun (Camb). 2017 Jun 29;53(53):7

Insert

ffDronpa-F

Tags

6xHis, T7 tag, and Xpress tag

Expression

Bacterial

Mutation

K45I, V60A, F173V

Promoter

T7

Available Since

Sept. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pT7_circRAJ31

Plasmid

#69931

Purpose

Expresses RAJ31 riboregulator encased in a permuted intron exon ribozyme, which circularises the riboregulator. Under control of a T7 promoter.

Depositor

Alfonso Jaramillo

Article

Rostain et al Biodes Res. 2020 Sep 12;2020:1916789. do

Insert

pT7_circRAJ31

Use

Synthetic Biology

Promoter

T7

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pAB170

Plasmid

#101669

Purpose

Opto-T7RNAP*(69), half expr.

Depositor

Mustafa Khammash

Article

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

Opto-T7RNAP*(69), half expr.

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pT7_circRAJ31_C873U

Plasmid

#69932

Purpose

Expresses RAJ31 riboregulator encased in a permuted intron exon ribozyme, which circularises the riboregulator. This mutant is deficient in circularisation. Under control of a T7 promoter.

Depositor

Alfonso Jaramillo

Article

Rostain et al Biodes Res. 2020 Sep 12;2020:1916789. do

Insert

pT7_circRAJ31_C873U

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available Since

Feb. 10, 2016

Availability

Academic Institutions and Nonprofits only

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B7N6P4

Plasmid

#163180

Purpose

Inducible expression of UniProt identifier B7N6P4

Depositor

Martin Engqvist

Article

Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

Insert

B7N6P4

Tags

10xHis and T7

Expression

Bacterial

Promoter

T7

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pTHSSd_10

Plasmid

#59950

Purpose

PK1F GFP reporter

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

Insert

sfGFP

Expression

Bacterial

Mutation

N/A

Promoter

PK1F

Available Since

Dec. 19, 2014

Availability

Academic Institutions and Nonprofits only

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pTHSSd_25

Plasmid

#59956

Purpose

PTac K1FR sigma fragment expression

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

Insert

K1FR sigma fragment

Expression

Bacterial

Mutation

N/A

Promoter

PTac

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pTHSSd_26

Plasmid

#59957

Purpose

PTac CGG sigma fragment expression

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

Insert

CGG sigma fragment

Expression

Bacterial

Mutation

N/A

Promoter

PTac

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pTHSSd_42

Plasmid

#59965

Purpose

beta core fragment expression

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

Insert

beta core fragment

Expression

Bacterial

Mutation

N/A

Promoter

PJ23105

Available Since

Dec. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pTHSSd_29

Plasmid

#59959

Purpose

PTac alpha fragment expression

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

Insert

alpha fragment

Expression

Bacterial

Mutation

N/A

Promoter

PTac

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pTHSSd_24

Plasmid

#59955

Purpose

PTac T3 sigma fragment expression

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

Insert

T3 sigma fragment

Expression

Bacterial

Mutation

N/A

Promoter

PTac

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pUC19 gene 60

Plasmid

#49090

Purpose

Template for generating full length gene 60 mRNA by in vitro transcription with T7 RNA pol

Depositor

Nils Walter

Article

Todd et al RNA. 2013 May;19(5):685-700. doi: 10.126

Insert

Bacteriophage T4 gene 60

Promoter

T7

Available Since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pMAT9

Plasmid

#112590

Purpose

Used for expression of protein in E. coli as Thrombin cleavable N-termina 6His-tag-MBP fusions and without NcoI site within MBP.

Depositor

Marko Hyvönen, Johan Peränen

Article

Peranen et al Anal Biochem. 1996 May 1;236(2):371-3.

Type

Empty backbone

Tags

6His-MBP

Expression

Bacterial

Available Since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pTHSSd_9

Plasmid

#59949

Purpose

PT3 GFP reporter

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

Insert

sfGFP

Expression

Bacterial

Mutation

N/A

Promoter

PT3

Available Since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pMAT10

Plasmid

#112591

Purpose

Used for expression of protein in E. coli as Thrombin cleavable N-termina 6His-tag-MBP fusions. Like pMAT9, but without the second BamHI site in the polylinker

Depositor

Marko Hyvönen, Johan Peränen

Article

Peranen et al Anal Biochem. 1996 May 1;236(2):371-3.

Type

Empty backbone

Tags

6His-MBP

Expression

Bacterial

Available Since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pTHSSd_11

Plasmid

#59951

Purpose

PCGG GFP reporter

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

Insert

sfGFP

Expression

Bacterial

Mutation

N/A

Promoter

PCCG

Available Since

Oct. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pUC19_TTE1564

Plasmid

#61000

Purpose

T7-transcription template for Tte mRNA used for in vitro translation

Depositor

Nils Walter

Article

Rinaldi et al Nat Commun. 2016 Jan 19;7:8976. doi: 10.

Insert

TTE1564 transcript

Use

Unspecified

Promoter

T7

Available Since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only

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EBOV Zeo-eGFP minigenome

Plasmid

#249393

Purpose

T7-driven EBOV minigenome vRNA with Zeo-eGFP reporter selection cassette

Depositor

Amy Kistler

Insert

Zeocin-eGFP fusion gene flanked by EBOV 5'UTR and 3'UTR

Expression

Bacterial

Promoter

T7

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pTE5415

Plasmid

#229030

Purpose

Expression vector for 10xHis-phi29 DNA polymerase (wild-type): PT7-lacO-g10leader-p2 wt-T7 terminator, CamR

Depositor

Tobias Erb

Article

Barthel et al bioRxiv 2025.07.08.663768; doi: 10.1101/

Insert

PT7-lacO-g10lead-10xHisTag-phi29 DNAP_wt

Tags

10x His-tag + Factor Xa recognition site

Expression

Bacterial

Promoter

PT7-lacO and T7 promoter

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pTE5444

Plasmid

#231064

Purpose

Expression vector for 10x-phi29 DNA polymerase (PovSak): PT7-lacO-g10leader-10xHis-p2 povsak-T7 terminator, CamR

Depositor

Tobias Erb

Article

Barthel et al bioRxiv 2025.07.08.663768; doi: 10.1101/

Insert

PT7-lacO-g10lead-10xHisTag-phi29 DNAP_PovSak

Tags

10x His-tag + Factor Xa recognition site

Expression

Bacterial

Promoter

PT7-lacO and T7 promoter

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pTE5448

Plasmid

#231065

Purpose

Expression vector for 10x-phi29 DNA polymerase (PovSakOk): PT7-lacO-g10leader-10xHis-p2 povsakok-T7 terminator, CamR

Depositor

Tobias Erb

Article

Barthel et al bioRxiv 2025.07.08.663768; doi: 10.1101/

Insert

PT7-lacO-g10lead-10xHisTag-phi29 DNAP_PovSakOk

Tags

10x His-tag + Factor Xa recognition site

Expression

Bacterial

Promoter

PT7-lacO and T7 promoter

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pJL1-sFLAG-p5-PRB4_R7-9-FETCH1

Plasmid

#210651

Purpose

In vitro expression of polyProline FETCH reporter from the T7 promoter

Depositor

Michael Jewett

Article

Willi et al ACS Synth Biol. 2024 Jul 19;13(7):2253-2

Insert

sFLAG-p5-PRB4_R7-9-FETCH1

Use

Synthetic Biology

Promoter

T7

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pJL1-sFLAG-p1-PRB4_R7-9-FETCH1

Plasmid

#210652

Purpose

In vitro expression of polyProline FETCH reporter from the T7 promoter

Depositor

Michael Jewett

Article

Willi et al ACS Synth Biol. 2024 Jul 19;13(7):2253-2

Insert

sFLAG-p1-PRB4_R7-9-FETCH1

Use

Synthetic Biology

Promoter

T7

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pJL1-sFLAG-a5-PRB4_R7-9A-FETCH1

Plasmid

#210653

Purpose

In vitro expression of polyProline FETCH control from the T7 promoter

Depositor

Michael Jewett

Article

Willi et al ACS Synth Biol. 2024 Jul 19;13(7):2253-2

Insert

sFLAG-a5-PRB4_R7-9A-FETCH1

Use

Synthetic Biology

Promoter

T7

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDA313

Plasmid

#195214

Purpose

Expression of superfolder GFP (sfGFP) by a hybrid T7 promoter with tetO.

Depositor

Rahul Sarpeshkar

Article

Deng et al Front Bioeng Biotechnol. 2022 Sep 28;10:

Insert

sfGFP with tetO and a hairpin

Expression

Bacterial

Promoter

T7

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 10,794 results for: T7