We narrowed to 14,621 results for: RING
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Plasmid#115205PurposeLentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S291A/S293A into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')DepositorInsertCR (CRISPR/Cas9-resistant)-PDHA2S291A/S293A (PDHA2 Human)
UseLentiviralMutationc.321C>T (silent when translated to protein, c…PromoterEF1alphaAvailable SinceMarch 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLD-puro-Cc-CR-PARK7WT-VA
Plasmid#115187PurposeLentiviral transduction and expression of CRISPR/Cas9-resistant PARK7WT into any mammalian cell (when using sgRNA seq agtacagtgtagccgtgatg)DepositorInsertCR (CRISPR/Cas9-resistant)-PARK7WT (PARK7 Human)
UseLentiviralTagsVersatile affinity tag (2XStreptactin II-6XHis-3X…Mutationc.150G>A (silent when translated to protein, c…PromoterCMVAvailable SinceMarch 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLD-puro-Cc-CR-PARK7V51G-VA
Plasmid#115188PurposeLentiviral transduction and expression of CRISPR/Cas9-resistant PARK7V51G into any mammalian cell (when using sgRNA seq agtacagtgtagccgtgatg)DepositorInsertCR (CRISPR/Cas9-resistant)-PARK7V51G (PARK7 Human)
UseLentiviralTagsVersatile affinity tag (2XStreptactin II-6XHis-3X…Mutationc.150G>A (silent when translated to protein, c…PromoterCMVAvailable SinceMarch 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLD-puro-Cc-CR-PARK7C53A-VA
Plasmid#115189PurposeLentiviral transduction and expression of CRISPR/Cas9-resistant PARK7C53A into any mammalian cell (when using sgRNA seq agtacagtgtagccgtgatg)DepositorInsertCR (CRISPR/Cas9-resistant)-PARK7C53A (PARK7 Human)
UseLentiviralTagsVersatile affinity tag (2XStreptactin II-6XHis-3X…Mutationc.150G>A (silent when translated to protein, c…PromoterCMVAvailable SinceMarch 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLD-puro-Cc-CR-PARK7H126A-VA
Plasmid#115190PurposeLentiviral transduction and expression of CRISPR/Cas9-resistant PARK7H126A into any mammalian cell (when using sgRNA seq agtacagtgtagccgtgatg)DepositorInsertCR (CRISPR/Cas9-resistant)-PARK7H126A (PARK7 Human)
UseLentiviralTagsVersatile affinity tag (2XStreptactin II-6XHis-3X…Mutationc.150G>A (silent when translated to protein, c…PromoterCMVAvailable SinceMarch 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLD-puro-Cc-CR-PARK7E163K-VA
Plasmid#115191PurposeLentiviral transduction and expression of CRISPR/Cas9-resistant PARK7E163K into any mammalian cell (when using sgRNA seq agtacagtgtagccgtgatg)DepositorInsertCR (CRISPR/Cas9-resistant)-PARK7E163K (PARK7 Human)
UseLentiviralTagsVersatile affinity tag (2XStreptactin II-6XHis-3X…Mutationc.150G>A (silent when translated to protein, c…PromoterCMVAvailable SinceMarch 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLEX307_CR-PDHA2
Plasmid#115199PurposeLentiviral transduction and expression of a CRISPR/Cas9-resistant PDHA2 into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')DepositorInsertCR (CRISPR/Cas9-resistant)-PDHA2WT (PDHA2 Human)
UseLentiviralMutationc.321C>T (silent when translated to protein, c…PromoterEF1alphaAvailable SinceMarch 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLEX307_CR-PDHA2S291A
Plasmid#115203PurposeLentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S291A into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')DepositorInsertCR (CRISPR/Cas9-resistant)-PDHA2S291A (PDHA2 Human)
UseLentiviralMutationc.321C>T (silent when translated to protein, c…PromoterEF1alphaAvailable SinceMarch 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
HS_NAM-B1
Plasmid#154064PurposeLevel M, P1 Golden Gate vector. OsAct::Hyg-nosT/HvHSP17::Cre-hspT/ZmUbi::loxP-GUS-loxP-NAM-B1-nosTDepositorInsertsHygromycin phosphotransferase
Cre recombinase
LoxP-flanked GUS and TtNAM-B1
UseCre/Lox and Synthetic Biology; Golden gateExpressionBacterial and PlantMutationThe TtNAM-B1 gene sequence was domesticated to re…PromoterHvHSP17, OsAct, and ZmUbiAvailable SinceSept. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-3-TRC8 513-664
Plasmid#185352PurposeRING domain-containing GST fusion protein for ubiquitination assays.DepositorAvailable SinceAug. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 T21R-F8.2(S54L+T127A)-T2A-mCherry
Plasmid#225588PurposeMammalian expression vector for TRIM21 RING-F8.2(S54L+T127A) anti-tau degrader with a self-cleaving T2A-mCherry fluorescent expression reporterDepositorInsertT21R-F8.2(S54L+T127A)-T2A-mCherry
TagsmCherryExpressionMammalianPromoterCMVAvailable SinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-SFFV-tagBFP-2A-ICP0-FxE
Plasmid#235539PurposeTo express tagBFP-2A-ICP0-FXE (ICP0 ∆106-149)DepositorInsertICP0-FxE
UseLentiviralMutationDeletion of the RING domain, ∆106-149.Available SinceJuly 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn T21R-F8.2 (S54L+T127A)-T2A-mCherry
Plasmid#225590PurposeAAV transgene plasmid with hSyn promoter for expression of TRIM21 RING-F8.2(S54L+T127A) anti-tau degrader with a self-cleaving T2A-mCherry fluorescent expression reporterDepositorInsertT21R-F8.2 (S54L+T127A)-T2A-mCherry
UseAAVTagsmCherryExpressionMammalianPromoterhSynAvailable SinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4TO T21R-F8.2 (S54L+T127A)-T2A-mCherry
Plasmid#225584PurposeMammalian expression vector for Doxycycline inducible expression of TRIM21 RING-F8.2(S54L+T127A) anti-tau degrader with a self-cleaving T2A-mCherry fluorescent expression reporterDepositorInsertT21R-F8.2 (S54L+T127A)-T2A-mCherry
TagsmCherryExpressionMammalianMutationT21R-F8.2 (S54L+T127A)PromoterCMVAvailable SinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMAL-PIAL2M-sim1
Plasmid#67908PurposeInducible expression of a mutant of PIAL2M where the SIM interacting motif 1 (VFDL 425-428) was substituted with alanines.DepositorInsertPIAL2 (AT5G41580 Mustard Weed)
TagsMBPExpressionBacterialMutationE281-A496; VEDL 425-428 AAAPromoterP-lacAvailable SinceAug. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV5D HA-PPM1H_M flap
Plasmid#236718PurposeExpresses PPM1H with PPM1M flap domain sequenceDepositorArticleTagsHAExpressionMammalianMutationPPM1H flap domain replaced with PPM1M flap domainPromoterCMVAvailable SinceMay 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV5D HA-PPM1M_H flap
Plasmid#236719PurposeExpresses PPM1M with PPM1H flap domain sequenceDepositorArticleTagsHAExpressionMammalianMutationPPM1M flap domain replaced with PPM1H flap domainPromoterCMVAvailable SinceMay 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRosettav2
Plasmid#115557PurposeTo determine correct antibiotic selection conditions in mammalian cells; standard for titering experimentsDepositorInsertHygromycin-Blasticidin-Puromycin-EGFP
UseLentiviralExpressionMammalianAvailable SinceOct. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSAH031
Plasmid#90330PurposePromoterless iLOV gene for recombineering, flanked by BsmbI restriction sites.DepositorInsertiLOV
TagsNonePromoterNoneAvailable SinceJuly 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
ATSR
Plasmid#50052Purposeexpresses t.gondii VWA+TSR1 domains in mammalian cellsDepositorInsertMIC2-ATSR
TagsHISExpressionMammalianMutationS158APromoterChick beta-actin promoterAvailable SinceFeb. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
proATSR
Plasmid#50053PurposeExpresses T.gondii prodomain+VWA+TSR1 domains in mammalian cellsDepositorInsertMIC2-proATSR
TagsHISExpressionMammalianMutationS158APromoterchick beta-actin promoterAvailable SinceDec. 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
(P5)efe
Plasmid#251384PurposeGene encoding Ethylene Forming Enzyme (Pseudomonas syringae)DepositorInsertefe
ExpressionBacterialAvailable SinceMarch 23, 2026AvailabilityAcademic Institutions and Nonprofits only -
pET-C
Plasmid#213496PurposeEncodes Node C of the three-node Turing circuitDepositorInsertslambda cI 5G6GP
mCherry
UseSynthetic BiologyTagsASV tag and LVA tagAvailable SinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
A5TSR
Plasmid#50358Purposeexpresses T.gondii MIC2(67-593) domains in mammalian cellsDepositorInsertMIC2-A5TSR
TagsHISExpressionMammalianMutationS158A, N357S, N463S, N470DPromoterCHICK beta-actin promoterAvailable SinceMarch 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
A6TSR
Plasmid#50359Purposeexpresses T.gondii MIC2(67-660) domains in mammalian cellsDepositorInsertMIC2-A6TSR
TagsHISExpressionMammalianMutationS158A, N357S, N463S, N470DPromoterCHICK beta-actin promoterAvailable SinceMarch 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCC1R
Plasmid#213497PurposeEncodes the receptor array and inducible aiiA gene of the three-node Turing circuitDepositorInsertsUseSynthetic BiologyAvailable SinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
MIC2
Plasmid#50050Purposeexpresses toxoplasma gondii MIC2 extracellular domain in mammalian cellsDepositorInsertMIC2
TagsHIS, SBP, and TEVExpressionMammalianMutationS158A, N357S, N463S, N470DPromoterCMVAvailable SinceApril 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
m2ap
Plasmid#50051Purposeexpresses toxoplasma gondii MIC2 associated protein (m2ap) in mammalian cellsDepositorInsertM2AP
TagsHISExpressionMammalianMutationstarts at aa47; N127R, S158A, S177N, N357S, N463S…PromoterCMVAvailable SinceMarch 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHelper_NoMutL_V2_gentR
Plasmid#205293PurposeHelper plasmid for ORBIT in E. coli. Expresses RecT for oligo recombineering and Bxb-1 for pInt incorporation. Has sacB for counter selection.DepositorArticleInsertCspRecT / Bxb-1
UseSynthetic BiologyAvailable SinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHelper_Ec1_V1_gentR
Plasmid#205291PurposeHelper plasmid for ORBIT in E. coli. Expresses RecT for oligo recombineering and Bxb-1 for pInt incorporation. Has sacB for counter selection.DepositorArticleInsertCspRecT / Bxb-1
UseSynthetic BiologyAvailable SinceAug. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHelper_Ec1_V1_ampR
Plasmid#205292PurposeHelper plasmid for ORBIT in E. coli. Expresses RecT for oligo recombineering and Bxb-1 for pInt incorporation. Has sacB for counter selection.DepositorArticleInsertCspRecT / Bxb-1
UseSynthetic BiologyAvailable SinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pL2Cas9
Plasmid#98841PurposeGenome engineering of virulent phagesDepositorInserttracr/Cas9
Available SinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pETM6
Plasmid#49795PurposeePathBrick vector for pathway engineering in E. coli.DepositorTypeEmpty backboneTagsS-tagExpressionBacterialPromoterT7Available SinceJan. 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDM4
Plasmid#49796PurposeePathBrick vector for pathway engineering in E. coli.DepositorTypeEmpty backboneTagsS-tagExpressionBacterialPromoterT7Available SinceJan. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pACM4
Plasmid#49797PurposeePathBrick vector for pathway engineering in E. coli.DepositorTypeEmpty backboneTagsS-tagExpressionBacterialPromoterT7Available SinceJan. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRSM3
Plasmid#49799PurposeePathBrick vector for pathway engineering in E. coli.DepositorTypeEmpty backboneTagsS-tagExpressionBacterialPromoterT7Available SinceFeb. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMB413a
Plasmid#20058DepositorInsertSperm Whale Myoglobin
ExpressionBacterialAvailable SinceJan. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCOM4
Plasmid#49798PurposeePathBrick vector for pathway engineering in E. coli.DepositorTypeEmpty backboneTagsS-tagExpressionBacterialPromoterT7Available SinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-TRP1-PGAL1-GFP (F64L, S65T, V163A)
Plasmid#53206PurposeN-terminal chromosomal tagging with stable GFP under control of GAL promoterDepositorInsertGAL1 promoter (GAL1 Budding Yeast)
UseYeast genomic targetingTagsGFP (F64L, S65T, V163A) and TRP1PromoterGAL1 promoterAvailable SinceJune 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-PGAL1-GFP (F64L, S65T, V163A)
Plasmid#53205PurposeN-terminal chromosomal tagging with stable GFP under control of GAL promoterDepositorInsertGAL1 promoter (GAL1 Budding Yeast)
UseYeast genomic targetingTagsGFP (F64L, S65T, V163A) and kanMX6PromoterGal1 promoterAvailable SinceMay 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMOD4 RT-G
Plasmid#19184DepositorInsertrpsL tetA
UseRecombineeringTags50 nucleotides of FLAG-GFPAvailable SinceJan. 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-HIS3MX6-PGAL1-GFP (F64L, S65T, V163A)
Plasmid#53207PurposeN-terminal chromosomal tagging with stable GFP under control of GAL promoterDepositorInsertGAL1 promoter (GAL1 Budding Yeast)
UseYeast genomic targetingTagsGFP (F64L, S65T, V163A) and HIS3MX6PromoterGAL1 promoterAvailable SinceMay 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNitET-SacB-kan
Plasmid#107692PurposeExpression of the phage Che9c RecET system for Recombineering in mycobacteriaDepositorInsertsChe9 phage RecET
PNit2-NitR
SacRB
ExpressionBacterialAvailable SinceMay 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDF-B-pC
Plasmid#213495PurposeEncodes Node B of the three-node Turing circuitDepositorUseSynthetic BiologyTagsASV tag and LVA tagAvailable SinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKM342
Plasmid#71486PurposeContains loxP-hyg-loxP cassette for recombineering; HygR, AmpR.DepositorInsertloxP-hyg-loxP cassette
UseSource of loxp-hyg-loxp for pcr amplification.ExpressionBacterialAvailable SinceApril 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKM343
Plasmid#71487PurposeContains loxP-hyg-cat-loxP cassette for recombineering; CamR, HygR.DepositorInsertloxP-hyg-cat-loxP
UseSource of loxp-hyg-cat- loxp for pcr amplificatio…ExpressionBacterialAvailable SinceJune 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMOD4 galK-G
Plasmid#19182DepositorAvailable SinceJan. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
pBBR1-kan-Ptet-RecETh1h2h3h4_agrob6
Plasmid#180428PurposeGenome engineering of AgrobacteriumDepositorInsertRecETh1h2h3h4_agrob6
ExpressionBacterialAvailable SinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCOLA-A-pC-tac
Plasmid#213494PurposeEncodes Node A of the three-node Turing circuitDepositorAvailable SinceFeb. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJA252
Plasmid#21512DepositorInsertpmex-5 (w/o ATG)
UseMultisite gatewayAvailable SinceAug. 11, 2009AvailabilityAcademic Institutions and Nonprofits only