We narrowed to 5,795 results for: plasmid dna
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Plasmid#193998Purposeplasmid encoding myc-tagged OGA(GS-544)C181_NESDepositorInsertmyc-OGA(GS-544)C181_NES
UseTagsmycExpressionMammalianMutationPromoterAvailable SinceJan. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag-Tet1 CD
Plasmid#70129PurposeExpress flag-tagged mouse Tet1 catalytic domain.DepositorInsertTen-Eleven Translocation 1 (Tet1 Mouse)
UseTagsflagExpressionMammalianMutationThe plasmid expresses TET1 catalytic domain.PromoterAvailable SinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag-Tet2 CD
Plasmid#72219PurposeExpress flag-tagged mouse Tet2 catalytic domain.DepositorInsertTen-Eleven Translocation 2 (Tet2 Mouse)
UseTagsflagExpressionMammalianMutationThe plasmid expresses TET2 catalytic domain.PromoterAvailable SinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PBZ-mRuby2
Plasmid#110650Purposeexpresses a PAR-binding (PBZ) domain fused to mRuby2DepositorInsertPBZ domain from APLF, amino acids 371-451 (APLF Human)
UseTagsmRuby2ExpressionMammalianMutationPromoterAvailable SinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EFNB2-RBD-Fc (WT)
Plasmid#200978PurposeMammalian expression plasmid for soluble EFNB2 (wild type) fused to IgG1 FcDepositorInsertEFNB2 (EFNB2 Human)
UseTagsIgG1 FcExpressionMammalianMutationSoluble Receptor-Binding DomainPromoterCMVAvailable SinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
FLAG-HA-BLMP-1-pcDNA3.1-
Plasmid#52512Purposeexpresses C. elegans BLMP-1 in mammalian cells with FLAG-HA tag at N-terminusDepositorInsertBlmp-1 (blmp-1 Nematode)
UseTagsFLAG-HAExpressionMammalianMutationN75S mutation compared GenBank reference sequence…PromoterCMVAvailable SinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sPDGFRalpha-Fc (Legacy)
Plasmid#136437PurposeMammalian expression plasmid for soluble ectodomain of PDGFR-alpha fused to IgG1 FcDepositorInsertPDGFRalpha (PDGFRA Human)
UseTagsFc region of IgG1 (a.a. Pro-217 to Lys-447) and L…ExpressionMammalianMutationExtracellular domain of PDGFR-alpha (a.a. 1-524)PromoterCMVAvailable SinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-H2B-AID-EYFP
Plasmid#47329Purposefor plasmid integration using the Flp-In system. AID at C-terminalDepositorAvailable SinceAug. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
GFP-pcDNA3-PKCgamma-cys1Acys1B
Plasmid#21190DepositorInsertPKCgamma-cys1Acys1B (Prkcg Rat)
UseTagsGFPExpressionMammalianMutationPKCgamma-cys1Acys1B (aa35 to aa152 of rat PKCgamm…PromoterAvailable SinceJuly 15, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT/TO Plk4 ∆24-mCherry
Plasmid#80271Purposemammalian expression plasmid for Plk4 with multi-phospho degron deletedDepositorInsertPlk4 (PLK4 Human)
UseTagsmCherryExpressionMammalianMutationdeletion of multi-phospho degron (amino acids 282…PromoterCMVAvailable SinceAug. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mas-GRK2RH-Nluc
Plasmid#158605PurposePlasmid expressing GRK2(RH)-Nluc in mammalian cellsDepositorInsertmyristic acid attachment peptide (mas; MGSSKSKTSNS) - linker (GIKLGG) - GRK2 RH domain - linker (GGTGGS) - Nluc
UseTagsExpressionMammalianMutationPromoterCMVAvailable SinceJune 29, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3.1(+)/AUG-nLuc-3XFLAG
Plasmid#127299PurposeExpress 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codonDepositorInsertnanoLuciferase
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable SinceJune 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/CUG-nLuc-3XFLAG
Plasmid#127300PurposeExpress 3xFLAG tagged nanoLuciferase (nLuc) from CUG start codonDepositorInsertnanoLuciferase
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable SinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/GUG-nLuc-3XFLAG
Plasmid#127301PurposeExpress 3xFLAG tagged nanoLuciferase (nLuc) from GUG start codonDepositorInsertnanoLuciferase
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable SinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/UUG-nLuc-3XFLAG
Plasmid#127302PurposeExpress 3xFLAG tagged nanoLuciferase (nLuc) from UUG start codonDepositorInsertnanoLuciferase
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable SinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HCMVgL-FLAG (Merlin)
Plasmid#136450PurposeMammalian expression plasmid for HCMV glycoprotein L (Merlin strain) with FLAG tagDepositorInsertglycoprotein L (UL115 Human betaherpesvirus 5)
UseTagsShort SGGSG linker and FLAG tagExpressionMammalianMutationCodon-optimized for human cell expression.PromoterCMVAvailable SinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT/TO Plk4 KD ∆24-mCherry
Plasmid#80272Purposemammalian expression plasmid for kinase dead Plk4 with multi-phospho degron deletedDepositorInsertPlk4 (PLK4 Human)
UseTagsmCherryExpressionMammalianMutationdeletion of multi-phospho degron (amino acids 282…PromoterCMVAvailable SinceAug. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2 mir-1-1 reporter mir30-wt
Plasmid#46695DepositorInsertmir30-wt (MIR30A Human, Synthetic)
UseRNAiTagsV5 and hsa-mir-1-1ExpressionMammalianMutationPromoterCMVAvailable SinceJune 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 JDM16-GFPnb-mCh
Plasmid#235667PurposeExpresses designed inhibitor of Hsp70 tagged with a GFP nano body and mCherryDepositorInsertJDM16-GFPnb-mCh (Mch Synthetic)
UseTagsGFP nanobody and mCherryExpressionMammalianMutationPromoterCMVAvailable SinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mas-PRGRH-Nluc
Plasmid#158608PurposePlasmid expressing PRG(RH)-Nluc in mammalian cellsDepositorInsertmyristic acid attachment peptide (mas; MGSSKSKTSNS) - linker (GIKLGG) - PDZ RhoGEF RH domain - linker (GGTGGS) - Nluc
UseTagsExpressionMammalianMutationPromoterCMVAvailable SinceJuly 20, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3.1-mas-p115RH-Nluc
Plasmid#158607PurposePlasmid expressing p115(RH)-Nluc in mammalian cellsDepositorInsertmyristic acid attachment peptide (mas; MGSSKSKTSNS) - linker (GIKLGG) - p115RhoGEF RH domain - linker (GGTGGS) - Nluc
UseTagsExpressionMammalianMutationPromoterCMVAvailable SinceJune 29, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3.1(+)/AUU-nLuc-3XFLAG
Plasmid#127305PurposeExpress 3xFLAG tagged nanoLuciferase (nLuc) from AUU start codonDepositorInsertnanoLuciferase
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable SinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/AGG-nLuc-3XFLAG
Plasmid#127304PurposeExpress 3xFLAG tagged nanoLuciferase (nLuc) from AGG start codonDepositorInsertnanoLuciferase
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable SinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/ACG-nLuc-3XFLAG
Plasmid#127303PurposeExpress 3xFLAG tagged nanoLuciferase (nLuc) from ACG start codonDepositorInsertnanoLuciferase
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable SinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
miR-223-RFP pCDNA
Plasmid#97142Purposeexpression of miR-223 and TagRFPDepositorInsertmiR-223
UseTagsTagRFPExpressionMammalianMutationPromoterAvailable SinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2 mir-1-1 reporter mir193b-wt
Plasmid#46734DepositorInsertmir193b-wt (MIR193B Human, Synthetic)
UseRNAiTagsV5 and hsa-mir-1-1ExpressionMammalianMutationPromoterCMVAvailable SinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_DNASE2_WT
Plasmid#81815PurposeGateway Donor vector containing DNASE2 , part of the Target Accelerator Plasmid Collection.DepositorAvailable SinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_DNAJC12_WT
Plasmid#81974PurposeGateway Donor vector containing DNAJC12, part of the Target Accelerator Plasmid Collection.DepositorAvailable SinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_DNASE2_p.Y182H
Plasmid#81374PurposeGateway Donor vector containing DNASE2 , part of the Target Accelerator Plasmid Collection.DepositorAvailable SinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_DNAJC12_p.R178C
Plasmid#81301PurposeGateway Donor vector containing DNAJC12 , part of the Target Accelerator Plasmid Collection.DepositorInsertDNAJC12 (DNAJC12 Human)
UseGateway entry vectorTagsExpressionMutationR178CPromoterNoneAvailable SinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3_c-MYC
Plasmid#26818DepositorInsert5'UTR-c-MYC-3'UTR (MYC Human)
UseTagsExpressionMammalianMutationSequence begins from second ATG; Synthetic UTR se…PromoterAvailable SinceNov. 17, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3.1 NL-BCL2L1
Plasmid#113458PurposeNanoLuc-BCL2L1 expression vector (positive control together with pcDNA3.1 PA-mCit-BAD)DepositorInsertBCL2 like 1 (BCL2L1 Human)
UseLuciferaseTagscmyc-NanoLucExpressionMammalianMutationPromoterCMVAvailable SinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MF3958 Heavy chain-SpyTag003
Plasmid#216303PurposeExpresses anti-HER2 MF3958 Heavy chain-SpyTag003 in mammalian cells. To be paired with MF3958 light chain to form the anti-HER2 MF3958 FabDepositorInsertMF3958 Heavy chain-SpyTag003
UseAffinity Reagent/ AntibodyTagsHis6 tag, Signal peptide, and SpyTag003ExpressionMammalianMutationPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-39S Heavy chain-SpyTag003
Plasmid#216296PurposeExpresses anti-HER2 39S Heavy chain-SpyTag003 in mammalian cells. To be paired with 39S light chain to form the anti-HER2 39S FabDepositorInsert39S Heavy chain-SpyTag003
UseAffinity Reagent/ AntibodyTagsHis6 tag, Signal peptide, and SpyTag003ExpressionMammalianMutationPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)Gα15-244-NLuc
Plasmid#207154PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNA15 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2DepositorInsertGNA15 (GNA15 Human)
UseTagsExpressionMammalianMutationNLuc inserted at position E(244)NPromoterAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HCMVgL-FLAG (TB40/E)
Plasmid#136449PurposeMammalian expression plasmid for HCMV glycoprotein L (TB40/E strain) with FLAG tagDepositorInsertglycoprotein L (UL115 Human betaherpesvirus 5)
UseTagsShort SGGSG linker and FLAG tagExpressionMammalianMutationCodon-optimized for human cell expression.PromoterCMVAvailable SinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HCMVgH-8his (Merlin)
Plasmid#136447PurposeMammalian expression plasmid for HCMV glycoprotein H (Merlin strain) extracellular domain with 8his purification tagDepositorInsertglycoprotein H (UL75 Human betaherpesvirus 5)
UseTagsShort GGSG linker and 8his tagExpressionMammalianMutationExtracellular domain (a.a. 1-716). Codon-optimize…PromoterCMVAvailable SinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag-Tet3 CD Mut
Plasmid#72222PurposeExpress flag-tagged mouse Tet3 catalytic domain - catalytic dead mutant.DepositorInsertTen-Eleven Translocation 3
UseTagsflagExpressionMammalianMutationThe plasmid expresses TET3 catalytic domain (cata…PromoterCMVAvailable SinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA_SZ1-RGG-GFP-RGG
Plasmid#124932PurposeExpression of SZ1-RGG-GFP-RGGDepositorInsertSYNZIP1-RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
UseTagsGFPExpressionMammalianMutationPromoterAvailable SinceJune 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-GFP-EBA175-Flag
Plasmid#154970PurposeExpresses N-terminally GFP-tagged EBA175 (residues 1284-1462) from pcDNA3.1. Please note: Plasmid contains a Stop codon prior to the C-terminal Flag tag.DepositorInsertPfEBA175
UseTagsGFP and signal peptide (residues 1 to 32) from TG…ExpressionMammalianMutationinsert codes for EBA175 residues 1284 to 1462PromoterCMVAvailable SinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HCMVgO-8his (Merlin)
Plasmid#136454PurposeMammalian expression plasmid for HCMV glycoprotein O (Merlin strain) with an 8his tagDepositorInsertglycoprotein O (UL74 Human betaherpesvirus 5)
UseTagsLinker and 8his tag (GGSGHHHHHHHH, includes BamHI…ExpressionMammalianMutationCodon-optimized for human cell expression.PromoterCMVAvailable SinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-Lin28A-mCCHC
Plasmid#51372DepositorInsertLin28A (LIN28A Human)
UseTagsFLAGExpressionMammalianMutationCCHC domain mutant (H147A, H169A)PromoterCMVAvailable SinceMarch 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/HP + AUG-nLuc-3XFLAG
Plasmid#127322PurposeExpress 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon from mRNA with hairpin in 5' UTRDepositorInsertnanoLuciferase
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable SinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)Gαq-124-NLuc
Plasmid#207153PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAQ tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2DepositorInsertGNAQ (GNAQ Human)
UseTagsExpressionMammalianMutationNLuc inserted at position F(124)EPromoterAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2 mir-1-1 reporter mir30-5
Plasmid#46692DepositorInsertmir30-5 (MIR30A Human, Synthetic)
UseRNAiTagsV5 and hsa-mir-1-1ExpressionMammalianMutationsee sequence and publicationPromoterCMVAvailable SinceJune 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-mNeonGreen-NUT
Plasmid#204693PurposeThis plasmid allows for inducible expression of full-length NUT tagged with mNeonGreen, in mammalian cells.DepositorAvailable SinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/HP + GUG-nLuc-3XFLAG
Plasmid#127323PurposeExpress 3xFLAG tagged nanoLuciferase (nLuc) from GUG start codon from mRNA with hairpin in 5' UTRDepositorInsertnanoLuciferase
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable SinceJuly 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2 mir-1-1 reporter mir193b-3
Plasmid#46733DepositorInsertmir193b-3 (MIR193B Human, Synthetic)
UseRNAiTagsV5 and hsa-mir-1-1ExpressionMammalianMutationsee sequence and publicationPromoterCMVAvailable SinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2 mir-1-1 reporter mir193b-2
Plasmid#46732DepositorInsertmir193b-2 (MIR193B Human, Synthetic)
UseRNAiTagsV5 and hsa-mir-1-1ExpressionMammalianMutationsee sequence and publicationPromoterCMVAvailable SinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2 mir-1-1 reporter mir193b-1
Plasmid#46731DepositorInsertmir193b-1 (MIR193B Human, Synthetic)
UseRNAiTagsV5 and hsa-mir-1-1ExpressionMammalianMutationsee sequence and publicationPromoterCMVAvailable SinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only