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pCS2+IMS-HyPer7

Plasmid

#136469

Purpose

Mammalian expression of mitochondrial intermembrane space targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

Depositor

Vsevolod Belousov

Publication

Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

Insert

HyPer7

Tags

SMAC/DIABLO

Expression

Mammalian

Promoter

CMV, SP6

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pCAG-G-Flamp2

Plasmid

#192782

Purpose

green biosensor for cAMP in mammalian cells

Depositor

Jun Chu

Publication

Liu et al Front Pharmacol. 2022 May 12;13:902290.

Insert

G-Flamp2

Tags

His tag

Expression

Mammalian

Promoter

CAG

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pECE-HA-AMPKalpha2 wt

Plasmid

#31654

Depositor

Anne Brunet

Publication

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

protein kinase, AMP-activated, alpha 2 catalytic subunit (PRKAA2 Human)

Tags

HA (YDVPDYASL)

Expression

Mammalian

Available since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pENTR-ERKKTRClover

Plasmid

#59138

Purpose

ORF encoding ERK KTR mClover flanked by Gateway sequences

Depositor

Markus Covert

Publication

Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

Insert

ERK Kinase Translocation Reporter (MAPK1 Mouse, Human)

Available since

Sept. 3, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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pcDNA-Flag-Tet3

Plasmid

#60940

Purpose

Expresses Tet3 in mammalian cells

Depositor

Yi Zhang

Publication

Wang et al Cell Rep. 2014 Jan 30;6(2):278-84. doi:

Insert

Ten-Eleven Translocation 3

Tags

flag

Expression

Mammalian

Promoter

CMV

Available since

Nov. 10, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pMXs-3XFLAG-EGFP-OMP25

Plasmid

#83354

Purpose

For tagging mitochondria with Sigma FLAG epitopes

Depositor

David Sabatini

Publication

Chen et al Cell. 2016 Aug 25;166(5):1324-1337.e11.

Insert

3XFLAG-EGFP-OMP25

Use

Retroviral

Expression

Mammalian

Available since

Sept. 26, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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MaLionR

Plasmid

#113908

Purpose

Red ATP sensor

Depositor

Tetsuya Kitaguchi

Publication

Arai et al Angew Chem Int Ed Engl. 2018 Jun 27. doi

Insert

MaLionR

Expression

Mammalian

Available since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pRK7-HA-S6K1-KR

Plasmid

#8985

Depositor

John Blenis

Publication

Schalm et al Curr Biol. 2002 Apr 16. 12(8):632-9.

Insert

S6K1 (Rps6kb1 Rat)

Tags

HA

Expression

Mammalian

Mutation

K100R, kinase-inactive

Available since

Jan. 4, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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-1748 human cyclin D1 promoter pGL3Basic

Plasmid

#32726

Depositor

Frank McCormick

Publication

Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

Insert

CCND1 promoter (CCND1 Human)

Use

Luciferase; Reporter gene

Promoter

-1748CCND1 promoter

Available since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pAAVS1-NC-CRISPRi (Gen3)

Plasmid

#73499

Purpose

Constitutive CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus

Depositor

Bruce Conklin

Publication

Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

Insert

dCas9-KRAB

Use

CRISPR

Tags

HA, KRAB, and NLS

Expression

Mammalian

Mutation

D10A, H840A (catalytically deactivated Cas9)

Promoter

CAG

Available since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pT3TS-Tol2

Plasmid

#31831

Depositor

Stephen Ekker

Publication

Balciunas et al PLoS Genet. 2006 Nov 10;2(11):e169. Epub

Insert

Tol2 Transposase

Use

Cloning vector

Promoter

Lac

Available since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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c-myc promoter (Del 4)

Plasmid

#16604

Depositor

Bert Vogelstein

Publication

He et al Science. 1998 Sep 4. 281(5382):1509-12.

Insert

c-myc promoter (Del 4) (MYC Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Del-4 (PvuII->PvuII). The first PvuII site is …

Available since

March 24, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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Green cGull

Plasmid

#86867

Purpose

Green fluorescent probe for cGMP

Depositor

Tetsuya Kitaguchi

Publication

Matsuda et al ACS Sens. 2017 Jan 27;2(1):46-51. doi: 1

Insert

Green cGull

Expression

Mammalian

Promoter

CMV

Available since

Feb. 23, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCMV-lyso-pHoenix

Plasmid

#70112

Purpose

Expression of lyso-pHoenix in mammalian cells for optogenetic acidification of lysosomes

Depositor

Christian Rosenmund

Publication

Rost et al Nat Neurosci. 2015 Nov 9. doi: 10.1038/n

Insert

lyso-pHoenix (Cd63 Halorubrum sodomense, Entacmaea quadricolor, Aequorea victoria, Mouse, Rat)

Tags

Arch3, H+/K+ ATPase beta-subunit, HA-Tag, mKate2,…

Expression

Mammalian

Promoter

CMV

Available since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCIP-Flag-TfebAA

Plasmid

#79014

Purpose

For mammalian expression of a Flag-tagged, constitutively active version (S142A, S211A) of Tfeb.

Depositor

Reuben Shaw

Publication

Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

Insert

Tfeb (Tfeb Mouse)

Tags

Flag

Expression

Mammalian

Mutation

S142A, S211A

Promoter

pCAGGs

Available since

July 15, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pSOUP

Plasmid

#165419

Purpose

The pSOUP helper plasmid needed for replication of all pGreen (Hellens et al., 2000) based vectors in Agrobacterium

Depositor

Wendy Harwood, John Innes Centre - Wheat Transformation and Genome Editing

Publication

Hayta et al Curr Protoc. 2021 Mar;1(3):e58. doi: 10.

Insert

Rep A

Use

Helper plasmid required for replication for all p…

Available since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pcDNA3-Tet1

Plasmid

#60938

Purpose

Expresses Tet1 in mammalian cells

Depositor

Yi Zhang

Publication

Wang et al Cell Rep. 2014 Jan 30;6(2):278-84. doi:

Insert

Ten-Eleven Translocation 1 (Tet1 Mouse)

Tags

flag

Expression

Mammalian

Promoter

CMV

Available since

Nov. 10, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pCMV Flag WT-PTEN

Plasmid

#22231

Depositor

Hong Wu

Publication

Mosessian et al J Biol Chem. 2009 Oct 30;284(44):30159-6

Insert

PTEN (PTEN Human)

Tags

Flag

Expression

Mammalian

Available since

Feb. 19, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pcDNA3.1 EYFP H148Q/I152L

Plasmid

#25872

Depositor

Peter Haggie

Publication

Galietta et al FEBS Lett. 2001 Jun 22. 499(3):220-4.

Insert

EYFP H148Q/I152L

Expression

Mammalian

Mutation

EYFP from pEYFP (Clontech) with H148Q and I152L m…

Available since

Aug. 16, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pLentiPGK DEST H2B-iRFP670

Plasmid

#90237

Purpose

Lentiviral vector to express H2B-iRFP670 under PGK promoter

Depositor

Markus Covert

Publication

Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

Insert

H2B

Use

Lentiviral

Tags

iRFP670

Expression

Mammalian

Promoter

PGK

Available since

March 17, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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