We narrowed to 18,890 results for: Tec

1. pR26 GFP Dest

   Plasmid

   #74283

   Purpose

   Gene targeting vector for the mouse Rosa26 locus, including a loxP flanked stop cassette and GFP, for gateway cloning

   Depositor

   Ralf Kuehn

   Article

   Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

   Insert

   Rosa26 5-homology region
   

   Available Since

   April 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. CP1041-ABEmax

   Plasmid

   #119808

   Purpose

   A-to-G base editor

   Depositor

   David Liu

   Article

   Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

   Insert

   ecTadA-ecTadA*(7.10)-nSpCas9 (CP1041)
   

   Expression

   Mammalian

   Available Since

   May 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. BiP-mCherry

   Plasmid

   #62233

   Purpose

   Hamster BiP/GRP78/HSPA5 fused to mCherry. Useful for studying BiP mobility and trafficking in live cells.

   Depositor

   Erik Snapp

   Article

   Lai et al Mol Biol Cell. 2010 Jun 15;21(12):1909-2

   Insert

   hamster BiP-mCherry-KDEL
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   cmv

   Available Since

   March 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCfB6633

   Plasmid

   #106162

   Purpose

   EasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6677 into Yarrowia lipolytica chromosomal location IntE_1, amp resistance

   Depositor

   Irina Borodina

   Article

   Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

   Insert

   unknown
   

   Expression

   Yeast

   Available Since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1(+)-ExRai-AKAR

   Plasmid

   #118405

   Purpose

   cpGFP-based excitation-ratiometric biosensor for monitoring Protein Kinase A activity.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   ExRai-AKAR
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCS2+ GFP-2xESP3I-IRES-mCherry

   Plasmid

   #230996

   Purpose

   Protein stability reporter construct for transient expression in mammalian cells. Stability of N-terminal GFP-fusion protein can be assessed by flow cytometry by normalizing to mCherry expression.

   Depositor

   Michael Rape

   Article

   Manford et al Cell. 2020 Oct 1;183(1):46-61.e21. doi:

   Type

   Empty backbone

   Tags

   AcGFP1

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAB1619 hU6-BpiI-Cas13bt1-DR

   Plasmid

   #176314

   Purpose

   hU6-BpiI-Cas13bt1-DR (crRNA expression)

   Depositor

   Feng Zhang

   Article

   Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103

   Insert

   Cas13bt1 crRNA direct repeat
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTlpA_mCherry_Txe/Axe

   Plasmid

   #229147

   Purpose

   Toxin-Antitoxin (Txe/Axe)

   Depositor

   Shrikrishnan Sankaran

   Article

   Dey et al Microb Biotechnol. 2023 Jun;16(6):1264-1

   Insert

   mCherry, Txe/Axe
   

   Expression

   Bacterial

   Promoter

   PtlpA (mCherry), Txe/Axe (Native)

   Available Since

   Jan. 29, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTK194

   Plasmid

   #59563

   Purpose

   TC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP XbaI attB1 Kozak mKate opal attB2 AscI (truncated E1) 3'UTR Poly A I-SceI

   Depositor

   Ron Weiss

   Article

   Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

   Insert

   SGP XbaI attB1 Kozak mKate opal attB2 AscI
   

   Available Since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDEST-hisMBP-AsCpf1-EC

    Plasmid

    #79007

    Purpose

    Expressing his-MBP tagged AsCpf1 (E.coli codon optimized)

    Depositor

    Jin-Soo Kim

    Article

    Hur et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

    Insert

    AsCpf1
    

    Tags

    6xHis-MBP-NLS and NLS-HA

    Expression

    Bacterial

    Promoter

    tac promoter

    Available Since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAF-HdrB3

    Plasmid

    #184908

    Purpose

    carrying CRISPR/Cas9 system for genome editing in Acidithiobacillus ferridurans

    Depositor

    Radhakrishnan Mahadevan

    Article

    Chen et al Environ Sci Technol. 2023 Aug 22;57(33):

    Insert

    Cas9-sgRNA-homology arms
    

    Use

    CRISPR; Broad-host-range

    Expression

    Bacterial

    Available Since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. U6-spTRE3G-CMV-mTagBFP2

    Plasmid

    #102854

    Purpose

    A plasmid encoding TRE3G sgRNA (for SpCas9) and mTagBFP

    Depositor

    Michael Lin

    Article

    Zhou et al ACS Chem Biol. 2017 Sep 29. doi: 10.1021

    Insert

    sp-TRE3G-sgRNA; mTagBFP2
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6 promoter

    Available Since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMJA284= pSin-Pur-MCS-Puro

    Plasmid

    #116877

    Purpose

    Lentiviral transfer vector containing a multible cloning site

    Depositor

    Marcos Alcocer

    Article

    Wang et al Biotechnol Lett. 2019 Oct;41(10):1095-11

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-Cas9-NRTH

    Plasmid

    #136925

    Purpose

    Expresses Cas9-NRTH in mammalian cells

    Depositor

    David Liu

    Article

    Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

    Insert

    Cas9-NRTH
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    see manuscript

    Promoter

    CMV

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMM114

    Plasmid

    #127211

    Purpose

    BeYDV replicon with constitutive Luc expression, WUS2 and IPT, sgRNA targeting PDS

    Depositor

    Daniel Voytas

    Article

    Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

    Insert

    WUS2, IPT, sgRNA, Luc
    

    Expression

    Plant

    Mutation

    codon optimized CDSs

    Available Since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAtetO_6xHis-Bst-LF

    Plasmid

    #145799

    Purpose

    Plasmid for the aTc-inducible expression of the large-fragment of Bst DNAP

    Depositor

    Andrew Ellington

    Article

    Bhadra et al mSphere. 2021 May 19;6(3):e00911-20. doi

    Insert

    Bst-LF
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    tet PA

    Available Since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. UPB-stomach (UPB1)

    Plasmid

    #180784

    Purpose

    Unique Projection Barcode (UPB1)-Stomach for Projection-seq

    Depositor

    Rui Chang

    Article

    Zhao et al Nature. 2022 Mar;603(7903):878-884. doi:

    Insert

    humanized ChR2-H134R (partial)
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. PrimeRoot.v2C-FLP

    Plasmid

    #201740

    Purpose

    For DNA insertion test in protoplast

    Depositor

    Caixia Gao

    Article

    Sun et al Nat Biotechnol. 2023 Apr 24. doi: 10.103

    Insert

    ePPE,FLP
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBJ1

    Plasmid

    #167130

    Purpose

    A gentamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pfrr promoter

    Depositor

    Akira Mine

    Article

    Matsumoto et al Plant Commun. 2021 Jul 20;3(1):100227. d

    Insert

    luxCDABE
    

    Use

    Luciferase

    Expression

    Bacterial

    Promoter

    Frr promoter

    Available Since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. NbV5-HALOTAG

    Plasmid

    #201482

    Purpose

    Indirect detection of V5-tagged protein using the HaloTag protein labeling technology.

    Depositor

    Patrick Giguère

    Article

    Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

    Insert

    NbV5-HALOTAG
    

    Tags

    HaloTag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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