We narrowed to 18,370 results for: Tec

1. Donor_eGP2AP_RC

   Plasmid

   #133784

   Purpose

   Library reporter vector/integration vector. Contains library cloning sites MluI and SpeI upstream of a terminator and downstream of a BxB1 attB site. Library integration occurs through BxB1 site.

   Depositor

   Sriram Kosuri

   Article

   Davis et al Cell Syst. 2020 Jul 22;11(1):75-85.e7. d

   Type

   Empty backbone

   Use

   Tags

   eGFP-P2A-Puro

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pconst #4-RFP

   Plasmid

   #81078

   Purpose

   constitutive promoter expressing RFP, biobrick construction

   Depositor

   Paul Adams, Jay Keasling

   Article

   Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

   Insert

   RFP
   

   Use

   P15a origin, constitutive promoter, kanr

   Tags

   Expression

   Mutation

   Promoter

   Constitutive Promoter - BBa_J23107

   Available since

   Aug. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOCC114

   Plasmid

   #118904

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal sortase site

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-HIS6-TEV-GG-NotI-ccdB-AscI-stop-HindIII cassette
   

   Use

   Tags

   HIS6, cleavable with TEV protease, two glycines

   Expression

   Insect

   Mutation

   Promoter

   polH

   Available since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. YPRCΔ15Ω1

   Plasmid

   #101710

   Purpose

   GoldenBraid vector pDGB2Ω1, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YPRCΔ15 solo LTR loci

   Depositor

   Elena Caro Bernat

   Article

   Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

   Type

   Empty backbone

   Use

   Yeast expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. U6-spTRE3G-CMV-mTagBFP2

   Plasmid

   #102854

   Purpose

   A plasmid encoding TRE3G sgRNA (for SpCas9) and mTagBFP

   Depositor

   Michael Lin

   Article

   Zhou et al ACS Chem Biol. 2017 Sep 29. doi: 10.1021

   Insert

   sp-TRE3G-sgRNA; mTagBFP2
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter

   Available since

   Nov. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTH

   Plasmid

   #56659

   Purpose

   Dual-expression vector for P. tricornutum

   Depositor

   Philip Weyman

   Article

   Weyman et al Plant Biotechnol J. 2014 Oct 10. doi: 10

   Insert

   KanR
   

   Use

   Synthetic Biology; Diatom expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEU3-NII-FLAGLICNot

   Plasmid

   #140184

   Purpose

   Used for producing recombinant proteins by wheat germ-based cell-free translation, contains T7 promoter, FLAG-tag and NotI restriction site

   Depositor

   Tamás Mészáros

   Article

   Nagy et al BMC Biotechnol. 2020 Mar 14;20(1):17. do

   Type

   Empty backbone

   Use

   Expression vector for a cell-free system

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   T7

   Available since

   June 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJL1-PD-4xDQNAT

   Plasmid

   #128391

   Purpose

   In vitro expression of H. influenzae protein D with C terminal 4xDQNAT glycosylation sites

   Depositor

   Michael Jewett

   Article

   Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

   Insert

   Protein D
   

   Use

   Tags

   4xDQNAT glycosylation tag and 6xHis tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDEST-hPDI

   Plasmid

   #60555

   Purpose

   enhance solubility and correct disulfide bond-formation of fused protein expressed in E.coli

   Depositor

   Han Choe

   Article

   Song et al Appl Biochem Biotechnol. 2013 May;170(1)

   Type

   Empty backbone

   Use

   Tags

   His6-hPDI

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCellFree_G03 RUVBL1

    Plasmid

    #67091

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    RUVBL1 (RUVBL1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. CP1300-ABEmax

    Plasmid

    #119810

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

    Insert

    ecTadA-ecTadA*(7.10)-nSpCas9 (CP1300)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pML3009

    Plasmid

    #193256

    Purpose

    Gamma-aminobutyric acid sensor expressing eYFP reporter

    Depositor

    Lauren Andrews

    Article

    Lebovich et al Front Bioeng Biotechnol. 2022 Aug 25;10:

    Inserts

    gabR
    eYFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    synthetic promoter P2 and synthetic promoter PGab…

    Available since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLPC-Flag-TRF2(delta)iDDR

    Plasmid

    #44577

    Depositor

    Eros Lazzerini Denchi

    Article

    Okamoto et al Nature. 2013 Feb 6. doi: 10.1038/nature1

    Insert

    TRF2ΔiDDR (TERF2 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    iDDR domain (aa 407-431) has been deleted

    Promoter

    Available since

    May 16, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pCMV-NES-MBP-cATOM(Ras)

    Plasmid

    #209711

    Purpose

    Mammalian expression of c-ATOM biosensor for HRas with nuclear export signal

    Depositor

    Stewart Loh

    Article

    Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

    Insert

    NES-MBP-cATOM(Ras)
    

    Use

    Tags

    NES-MBP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDYU1G/mRuby3–histone H1; γ-tubulin-mEGFP

    Plasmid

    #154303

    Purpose

    Expresses mRuby3–histone H1 and γ-tubulin-mEGFP from actin15 promoter in Dictyostelium discoideum

    Depositor

    Shigehiko Yumura

    Article

    Kondo et al Biotechniques. 2020 Feb;68(2):91-95. doi

    Inserts

    histone H1
    γ-tubulin
    

    Use

    Dictyostelium expression

    Tags

    mEGFP and mRuby3

    Expression

    Mutation

    Promoter

    actin15

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. enAsCas12a-mSA

    Plasmid

    #182954

    Purpose

    Express enAsCas12a-mSA

    Depositor

    Wen Xue

    Article

    Liang et al Nat Commun. 2022 Jan 21;13(1):437. doi:

    Insert

    enAsCas12a-mSA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAFNF-tdTomato

    Plasmid

    #125575

    Purpose

    Flp-dependent expression of tdTomato in mammalian cells.

    Depositor

    Takeshi Imai

    Article

    Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

    Insert

    tdTomato
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. LR (bact: HyPer)

    Plasmid

    #162069

    Purpose

    Used for the diffuse visualization of H2O2 production in cells.

    Depositor

    Philipp Niethammer

    Article

    Enyedi et al Nat Cell Biol. 2013 Sep;15(9):1123-30. d

    Insert

    beta actin 2 (actb2 Zebrafish)
    

    Use

    Gateway donor vector

    Tags

    HyPer

    Expression

    Mutation

    Promoter

    T3

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pVH613

    Plasmid

    #169602

    Purpose

    Tier-2 vector encoding PCREm4-driven Nluc and PmPGK1-driven Fluc expression (PCREm4-Nluc-pA::A2-pA::PmPGK1-Fluc-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    cAMP response element-controlled NanoLuc Luciferase expression cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PCREm4 / PPGK

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDEST-hPDIb′a′

    Plasmid

    #60556

    Purpose

    enhance solubility and correct disulfide bond-formation of fused protein expressed in E.coli

    Depositor

    Han Choe

    Article

    Song et al Appl Biochem Biotechnol. 2013 May;170(1)

    Type

    Empty backbone

    Use

    Tags

    His6-hPDIb'a' tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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