We narrowed to 6,994 results for: ef1a

1. pLenti-H3.3(K36M)-3AID-HA-2A-mTurquoiseBSD

   Plasmid

   #225703

   Purpose

   Lentiviral expression of AID degron tagged H3.3(K36M) and mTurquoise fused BlasticidinR

   Depositor

   Lacra Bintu

   Article

   Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

   Insert

   H3.3 (K36M) (H3-3A, H3-3B Human)
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   3xAID HA and T2A-mTurquoise

   Expression

   Mammalian

   Mutation

   K36M

   Promoter

   pEF1A

   Available since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAR014 Lenti pEF-dEnAsCas12a-NLS-VPR-3XHA-T2A-BSD

   Plasmid

   #195548

   Purpose

   dCas12a effector fusion for manipulating transcription

   Depositor

   Michael Bassik

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   Lenti pEF-dEnAsCas12a-NLS-VPR-3XHA-T2A-BSD
   

   Use

   CRISPR and Lentiviral

   Tags

   3XHA

   Expression

   Mammalian

   Mutation

   Promoter

   pEF1a

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. lenti.DTR.GFP

   Plasmid

   #201962

   Purpose

   Lentiviral vector that expresses diptheria toxin receptor (DTR) C-terminally fused to EGFP

   Depositor

   Bernhard Schmierer

   Article

   Fernandez Moro et al Nat Commun. 2023 Aug 18;14(1):5024. doi:

   Insert

   Diptheria Toxin Receptor
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAR028 Lenti pEF-dEnAsCas12a-NLS-ZNF705F KRAB-3XHA-T2A-BSD

   Plasmid

   #195545

   Purpose

   dCas12a effector fusion for manipulating transcription

   Depositor

   Michael Bassik

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   Lenti pEF-dEnAsCas12a-NLS-TopKRAB-3XHA-T2A-BSD
   

   Use

   CRISPR and Lentiviral

   Tags

   3XHA

   Expression

   Mammalian

   Mutation

   Promoter

   pEF1a

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLX_314-HRI-V5

   Plasmid

   #202434

   Purpose

   Expression of HRI

   Depositor

   William Hahn

   Article

   Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

   Insert

   HRI (EIF2AK1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJT282 Lenti pEF-dCas9-3XFLAG-N+Z+F-T2A-tagBFP-P2A-Blast

   Plasmid

   #187337

   Purpose

   dCas9 effector fusion for manipulating transcription

   Depositor

   Lacra Bintu, Michael Bassik

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   dCas9-3XFLAG-N+Z+F-T2A-tagBFP-P2A-Blast
   

   Use

   CRISPR and Lentiviral

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   pEF1a

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAR045 Lenti pEF-dEnAsCas12a-NLS-QNZF-3XHA-T2A-BSD

   Plasmid

   #195549

   Purpose

   dCas12a effector fusion for manipulating transcription

   Depositor

   Michael Bassik

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   Lenti pEF-dEnAsCas12a-NLS-QNZF-3XHA-T2A-BSD
   

   Use

   CRISPR and Lentiviral

   Tags

   3XHA

   Expression

   Mammalian

   Mutation

   Promoter

   pEF1a

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pPV-TetO-SpCas9-GR-iC-ERiH (CRONUS-Hygro)

   Plasmid

   #100597

   Purpose

   piggyBac vector expressing Dual-regulated Cas9 (Hygro resistance)

   Depositor

   Akitsu Hotta

   Article

   Ishida et al Sci Rep. 2018 Jan 10;8(1):310. doi: 10.1

   Inserts

   CRISPR Cas9 fused with human Glucocorticoid Receptor
   mCherry
   rtTA-M2
   

   Use

   Piggybac vector

   Tags

   Expression

   Mammalian

   Mutation

   Codon-optimized for human codon usage

   Promoter

   Dox-inducible TetO promoter and Human EEF1A1 prom…

   Available since

   April 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pPV-TetO-SpCas9-GR-iC-ERiP (CRONUS-Puro)

   Plasmid

   #100596

   Purpose

   piggyBac vector expressing Dual-regulated Cas9 (Puro resistance)

   Depositor

   Akitsu Hotta

   Article

   Ishida et al Sci Rep. 2018 Jan 10;8(1):310. doi: 10.1

   Inserts

   CRISPR Cas9 fused with human Glucocorticoid Receptor
   mCherry
   rtTA-M2
   

   Use

   Piggybac vector

   Tags

   Expression

   Mammalian

   Mutation

   Codon-optimized for human codon usage

   Promoter

   Dox-inducible TetO promoter and Human EEF1A1 prom…

   Available since

   April 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAR012 Lenti pEF-dEnAsCas12a-NLS-ZNF10 KRAB-3XHA-T2A-BSD

    Plasmid

    #195550

    Purpose

    dCas12a effector fusion for manipulating transcription

    Depositor

    Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    Lenti pEF-dEnAsCas12a-NLS-ZNF10 KRAB-3XHA-T2A-BSD
    

    Use

    CRISPR and Lentiviral

    Tags

    3XHA

    Expression

    Mammalian

    Mutation

    Promoter

    pEF1a

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLenti-H3.3(K27M)-3AID-HA-2A-mCherryBSD

    Plasmid

    #225698

    Purpose

    Lentiviral expression of AID degron tagged H3.3(K27M) and mCherry fused BlasticidinR

    Depositor

    Lacra Bintu

    Article

    Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

    Insert

    H3.3 (K27M) (H3-3A, H3-3B Human)
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    3xAID HA and T2A-mCherry

    Expression

    Mammalian

    Mutation

    K27M

    Promoter

    pEF1A

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJT218 lenti pEF-rTetR(SE-G72P)-3XFLAG-VP64-T2A-mCherry-BSD-WPRE

    Plasmid

    #188758

    Purpose

    Lentiviral backbone containing VP64 as a fusion with rTetR(SE-G72P)-3XFLAG

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    VP64
    

    Use

    Lentiviral

    Tags

    rTetR(SE-G72P)-3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    pEF1a

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAR013 Lenti pEF-dEnAsCas12a-NLS-VP64-3XHA-T2A-BSD

    Plasmid

    #195547

    Purpose

    dCas12a effector fusion for manipulating transcription

    Depositor

    Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    Lenti pEF-dEnAsCas12a-NLS-VP64-3XHA-T2A-BSD
    

    Use

    CRISPR and Lentiviral

    Tags

    3XHA

    Expression

    Mammalian

    Mutation

    Promoter

    pEF1a

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTRE-HRI-V5-IRES-BFP

    Plasmid

    #202435

    Purpose

    Lentiviral vector expression of HRI and TagBFP

    Depositor

    William Hahn

    Article

    Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

    Insert

    HRI (EIF2AK1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. p1.1-mCherry

    Plasmid

    #162772

    Purpose

    Fluorescent reporter for CHO expression studies

    Depositor

    Ivan Vorobiev

    Article

    Kovnir et al Bull Exp Biol Med. 2017 Jun;163(2):245-2

    Insert

    red fluorescent protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    consensus Kozak sequence (GCCGCCATGG) added befor…

    Promoter

    CHO EEF1A1 (Translation Elongation Factor 1 Alpha…

    Available since

    Aug. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p1.2-GS-eGFP

    Plasmid

    #162771

    Purpose

    Fluorescent reporter for CHO expression studies

    Depositor

    Ivan Vorobiev

    Article

    Kovnir et al Bull Exp Biol Med. 2017 Jun;163(2):245-2

    Insert

    enhanced green fluorescent protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    consensus Kozak sequence (GCCGCCATGG) added befor…

    Promoter

    CHO EEF1A1 (Translation Elongation Factor 1 Alpha…

    Available since

    Aug. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLEX307_PDHA2S293A

    Plasmid

    #115197

    Purpose

    Lentiviral transduction and expression of PDHA2S293A into any mammalian cell

    Depositor

    Mohan Babu

    Article

    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

    Insert

    PDHA2S293A (PDHA2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    p.S293A

    Promoter

    EF1alpha

    Available since

    March 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTF-ACE2s

    Plasmid

    #162786

    Purpose

    The extracellular domain of Angiotensin converting enzyme 2 (ACE2) expression

    Depositor

    Ivan Vorobiev

    Article

    Kolesov et al Diagnostics (Basel). 2022 Feb 3;12(2). p

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Use

    Tags

    10xHis, FLAG, and hTPA leader

    Expression

    Mammalian

    Mutation

    Promoter

    CHO EEF1A1 (Translation Elongation Factor 1 Alpha…

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. DNMT3ACD-CRY2-EGFP

    Plasmid

    #82556

    Purpose

    Encodes DMNT3 under LITE2.0 system

    Depositor

    Joseph Irudayaraj

    Article

    Lo et al Sci Rep. 2017 Feb 9;7:42047. doi: 10.103

    Inserts

    DNMT3A (DNMT3A Human)
    CRY2 (CRY2 Mustard Weed)
    EGFP
    

    Use

    AAV

    Tags

    2A, NLS(alpha-imp), and NLS-VP64

    Expression

    Mammalian

    Mutation

    CRY2PHR (N-terminal photolyase homology region do…

    Promoter

    EF-1alpha

    Available since

    Sept. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TET1CD-CRY2-EGFP

    Plasmid

    #82555

    Purpose

    Encodes TET1 under LITE2.0 system

    Depositor

    Joseph Irudayaraj

    Article

    Lo et al Sci Rep. 2017 Feb 9;7:42047. doi: 10.103

    Inserts

    TET1 (TET1 Human)
    CRY2 (CRY2 Mustard Weed)
    EGFP
    

    Use

    AAV

    Tags

    2A, NLS(alpha-imp), and NLS-VP64

    Expression

    Mammalian

    Mutation

    CRY2PHR (N-terminal photolyase homology region do…

    Promoter

    EF-1alpha

    Available since

    Sept. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLKO.1-shDnd1-No7

    Plasmid

    #70068

    Purpose

    TRC lentiviral vector for shRNA against mouse Dnd1 #7

    Depositor

    Thomas Tuschl

    Article

    Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

    Insert

    Dnd1 (Dnd1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Nov. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

22. pJT350 Lenti pEF-dCas9-3XFLAG-MBDa-T2A-tagBFP-P2A-Blast

    Plasmid

    #187347

    Purpose

    dCas9 effector fusion for manipulating transcription

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    dCas9-3XFLAG-MBDa-T2A-tagBFP-P2A-Blast
    

    Use

    CRISPR and Lentiviral

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    pEF1a

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pJT351 Lenti pEF-dCas9-3XFLAG-P66_CC-T2A-tagBFP-P2A-Blast

    Plasmid

    #187348

    Purpose

    dCas9 effector fusion for manipulating transcription

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    dCas9-3XFLAG-P66_CC-T2A-tagBFP-P2A-Blast
    

    Use

    CRISPR and Lentiviral

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    pEF1a

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pJT256 Lenti pEF-dCas9-3XFLAG-N+F-T2A-tagBFP-P2A-Blast

    Plasmid

    #187332

    Purpose

    dCas9 effector fusion for manipulating transcription

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    dCas9-3XFLAG-N+F-T2A-tagBFP-P2A-Blast
    

    Use

    CRISPR and Lentiviral

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    pEF1a

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pJT257 Lenti pEF-dCas9-3XFLAG-Z+F-T2A-tagBFP-P2A-Blast

    Plasmid

    #187333

    Purpose

    dCas9 effector fusion for manipulating transcription

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    dCas9-3XFLAG-Z+F+N-T2A-tagBFP-P2A-Blast
    

    Use

    CRISPR and Lentiviral

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    pEF1a

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pJT329 Lenti pEF-rTetR(SE-G72P)-3XFLAG-N+Z+F-T2A-mCherry-BSD-WPRE

    Plasmid

    #187339

    Purpose

    rTetR effector fusion for manipulating transcription

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    rTetR(SE-G72P)-3XFLAG-N+Z+F-T2A-mCherry-BSD-WPRE
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    pEF1a

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pJT252 Lenti pEF-dCas9-3XFLAG-ZN473_TAD-T2A-tagBFP-P2A-Blast

    Plasmid

    #187328

    Purpose

    dCas9 effector fusion for manipulating transcription

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    dCas9-3XFLAG-ZN473_TAD-T2A-tagBFP-P2A-Blast
    

    Use

    CRISPR and Lentiviral

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    pEF1a

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pJT253 Lenti pEF-dCas9-3XFLAG-FOXO3_TAD-T2A-tagBFP-P2A-Blast

    Plasmid

    #187329

    Purpose

    dCas9 effector fusion for manipulating transcription

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    dCas9-3XFLAG-FOXO3_TAD-T2A-tagBFP-P2A-Blast
    

    Use

    CRISPR and Lentiviral

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    pEF1a

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pJT254 Lenti pEF-dCas9-3XFLAG-NCOA3_TAD-T2A-tagBFP-P2A-Blast

    Plasmid

    #187330

    Purpose

    dCas9 effector fusion for manipulating transcription

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    dCas9-3XFLAG-NCOA3_TAD-T2A-tagBFP-P2A-Blast
    

    Use

    CRISPR and Lentiviral

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    pEF1a

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pJT255 Lenti pEF-dCas9-3XFLAG-N+Z-T2A-tagBFP-P2A-Blast

    Plasmid

    #187331

    Purpose

    dCas9 effector fusion for manipulating transcription

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    dCas9-3XFLAG-N+Z-T2A-tagBFP-P2A-Blast
    

    Use

    CRISPR and Lentiviral

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    pEF1a

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge