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pSB19-PapRS#34-OmpA

Plasmid

#231105

Purpose

Vector for bacterial expression of a protein carrying the non-canonical amino acid p-(phenylazo)-L-phenylalanine (encoding the OmpA signal sequence for periplasmic secretion)

Depositor

Arne Skerra

Article

Mayrhofer et al Nat Commun. 2024 Dec 18;15(1):10693. doi

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pEIAV-SIN6.1 CBLacZ-LucWm1A

Plasmid

#44167

Depositor

John Olsen

Article

Patel et al J Gene Med. 2013 Jan;15(1):51-62. doi: 1

Inserts

LacZ
firefly luciferase

Use

Lentiviral and Luciferase

Promoter

CB, CMV enhancer/chicken beta-actin promoter

Available Since

April 24, 2013

Availability

Academic Institutions and Nonprofits only

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pGL2_169

Plasmid

#199101

Purpose

Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pIJ101 ORI plus NGS barcode 15, gentamicin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available Since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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pFPNL1

Plasmid

#61651

Purpose

Gateway vector for generating nucleus-targeted fluorescent protein fusions in fungi

Depositor

Mark Farman

Article

Gong et al Mol Plant Microbe Interact. 2014 Nov 12.

Insert

monomeric red fluorescent protein

Expression

Plant

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only
pGL2_173

Plasmid

#199105

Purpose

Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pBBR1-UP ORI plus NGS barcode 21, gentamicin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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mTagRFP-pBAD

Plasmid

#54539

Purpose

Localization: Protein Expression Vector , Excitation: 555, Emission: 584

Depositor

Dmitriy Chudakov, Michael Davidson, Dorus Gadella

Article

Merzlyak et al Nat Methods. 2007 Jul;4(7):555-7. Epub 2

Type

Empty backbone

Tags

6xHis and mTagRFP

Expression

Bacterial

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pAJM.717

Plasmid

#108517

Purpose

PTet*-YFP Reporter. aTc sensor for use in Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

PTet*-YFP

Expression

Bacterial

Available Since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pAJM.716

Plasmid

#108518

Purpose

PBAD-YFP Reporter. Ara sensor for use in Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

PBAD-YFP

Expression

Bacterial

Available Since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pGL2_168

Plasmid

#199100

Purpose

Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

RSF1010 ORI plus NGS barcode 14, gentamicin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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pFA6a-mScarlet-H-mNeonGreen-kanMX (pKBJ009)

Plasmid

#173456

Purpose

Contains mScarletH-mNeonGreen tandem fluorescent protein timer

Depositor

Anton Khmelinskii

Article

Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

Insert

mScarlet-H-mNeonGreen

Expression

Yeast

Promoter

no promoter

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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Cip-pAct1-Mnase-SAT1

Plasmid

#162557

Purpose

For the C. auris MNase control strain, the URA3 auxotrophy marker of the CIp-pACT1-3xFLAG-MNase-SV40-CYC was replaced by SAT1

Depositor

Adnane Sellam

Article

Tebbji et al mSphere. 2020 Oct 14;5(5). pii: 5/5/e006

Insert

3FLAG-Mnase-SV40

Expression

Yeast

Promoter

Actine

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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MXS_BiTet

Plasmid

#62423

Purpose

MXS_chaining vector with BiTet

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

Bidirectional tetracycline inducible promoter

Use

Synthetic Biology

Available Since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

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mTagBFP2-Vinculin-23

Plasmid

#55334

Purpose

Localization: Focal Adhesions, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

Vinculin (VCL Human)

Tags

mTagBFP2

Expression

Mammalian

Mutation

V234L in Vinculin.

Promoter

CMV

Available Since

Oct. 22, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pSC101-GFPmut2-mScarlet-I TAG

Plasmid

#208188

Purpose

nonsense mistranslation, TAG stop

Depositor

Tanel Tenson

Article

Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

Inserts

GFPmut2
mScarlet-I

Use

Reporter

Expression

Bacterial

Mutation

Insertion 6TAG*(stop) 7AGT(Ser)

Promoter

Ptet+dnaK P1

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pSC101-GFPmut2-mScarlet-I TGA

Plasmid

#208189

Purpose

nonsense mistranslation, TGA stop

Depositor

Tanel Tenson

Article

Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

Inserts

GFPmut2
mScarlet-I

Use

Reporter

Expression

Bacterial

Mutation

Insertion 6TGA*(stop) 7AGT(Ser)

Promoter

Ptet+dnaK P1

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pSC101-GFPmut2-mScarlet-I TGATC

Plasmid

#208190

Purpose

nonsense mistranslation, TGA stop variant 2

Depositor

Tanel Tenson

Article

Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

Inserts

GFPmut2
mScarlet-I

Use

Reporter

Expression

Bacterial

Mutation

Insertion 6TGA*(stop) 7TCT(Ser)

Promoter

Ptet+dnaK P1

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pSC101-GFPmut2-mScarlet-I T7

Plasmid

#208191

Purpose

frameshift mistranslation, T7

Depositor

Tanel Tenson

Article

Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

Inserts

GFPmut2
mScarlet-I

Use

Reporter

Expression

Bacterial

Mutation

Frameshift insertion 6TTTTTTT

Promoter

Ptet+dnaK P1

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pSC101-GFPmut2-mScarlet-I A5

Plasmid

#208192

Purpose

frameshift mistranslation, A5

Depositor

Tanel Tenson

Article

Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

Inserts

GFPmut2
mScarlet-I

Use

Reporter

Expression

Bacterial

Mutation

Frameshift insertion 6AAAAA

Promoter

Ptet+dnaK P1

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pSC101-GFPmut2-mScarlet-I A4

Plasmid

#208193

Purpose

frameshift mistranslation, A4

Depositor

Tanel Tenson

Article

Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

Inserts

GFPmut2
mScarlet-I

Use

Reporter

Expression

Bacterial

Mutation

Frameshift insertion 6AAAA

Promoter

Ptet+dnaK P1

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pSC101-GFPmut2-mScarlet-I A6T

Plasmid

#208194

Purpose

frameshift mistranslation, A6T

Depositor

Tanel Tenson

Article

Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

Inserts

GFPmut2
mScarlet-I

Use

Reporter

Expression

Bacterial

Mutation

Frameshift insertion 6AAAAAAT

Promoter

Ptet+dnaK P1

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pSC101-GFPmut2-mScarlet-I C3T

Plasmid

#208195

Purpose

frameshift mistranslation, C3T

Depositor

Tanel Tenson

Article

Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

Inserts

GFPmut2
mScarlet-I

Use

Reporter

Expression

Bacterial

Mutation

Frameshift insertion 6CCCT

Promoter

Ptet+dnaK P1

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pSC101-GFPmut2-mScarlet-I 6Trp7Ser

Plasmid

#208186

Purpose

mistranslation GFP and mScarlet-I positive control with Trp insertion

Depositor

Tanel Tenson

Article

Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

Inserts

GFPmut2
mScarlet-I

Use

Reporter

Expression

Bacterial

Mutation

Insertion 6Trp7Ser

Promoter

Ptet+dnaK P1

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pHH0103_RAB5A_Ras

Plasmid

#109935

Purpose

Protein expression and purification of RAB5A_Ras

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

RAB5A_Ras

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pTn7-SCOUT13.G

Plasmid

#201001

Purpose

pLv1-pUC18R6KT-miniTn7-Tc-sfGFP, TcR, ApR

Depositor

Philip Poole

Article

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Insert

superfolder GFP

Expression

Bacterial

Promoter

J23104

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pC4-RHE-PAS (FRB-PAS)

Plasmid

#80002

Purpose

miRFP PAS domain fused to FRB

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

FRB

Tags

PAS domain of miRFP

Expression

Mammalian

Promoter

CMV

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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p1.1

Plasmid

#162737

Purpose

Protein expression in CHO cells

Depositor

Ivan Vorobiev

Article

Orlova et al BMC Biotechnol. 2014 Jun 14;14:56. doi:

Type

Empty backbone

Expression

Mammalian

Promoter

CHO EEF1A1 (Translation Elongation Factor 1 Alpha…

Available Since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

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TC1078-30

Plasmid

#164878

Purpose

30bp(mismatch17) cas13d gRNA for 1405 editing

Depositor

Tian Chi

Article

Liu et al EMBO J. 2020 Nov 16;39(22):e104748. doi:

Insert

gRNA for TC1405

Expression

Mammalian

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pBRHDAd-TKVenus

Plasmid

#26422

Depositor

Kohnosuke Mitani

Article

Suzuki et al Proc Natl Acad Sci U S A. 2008 Sep 16. 1

Insert

HSV-TK, CS2-Venus

Use

Adenoviral

Expression

Mammalian

Available Since

Jan. 31, 2011

Availability

Academic Institutions and Nonprofits only

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MXS_CMV

Plasmid

#62417

Purpose

MXS_chaining vector with CMV

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

CMV Promoter

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pERB217

Plasmid

#61500

Purpose

Const. Halotag-GFP-Mito + mCherry-DHFR

Depositor

David Chenoweth, Michael Lampson

Article

Ballister et al Nat Commun. 2014 Nov 17;5:5475. doi: 10.

Inserts

HaloTag-GFP-Mito
mCherry-DHFR

Tags

HaloTag and mCherry

Expression

Mammalian

Available Since

Feb. 19, 2015

Availability

Academic Institutions and Nonprofits only

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