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pSLQ8629 pHR-HREYBTATA-RR12EE345L-C term (406) dLbCpf1-2XNLS-miniVPR-mCherry-EFS-Puro-WPRE

Plasmid

#140227

Purpose

Hypoxia-inducible expression of leucine zipper with C terminal dCpf1 (Cas12a) 406-split half fused to miniVPR. Constitutive EFS-driven puromycin resistance.

Depositor

Stanley Qi

Article

Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

Insert

zipper with dLbCpf1 (Cas12a) C terminal half (406) fused to miniVPR

Use

Lentiviral

Expression

Mammalian

Promoter

HRE (Hypoxia inducible)

Available Since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

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MO70-hJunctate - EF-mutant

Plasmid

#79597

Purpose

Transient and retroviral expression

Depositor

Yousang Gwack

Article

Srikanth et al Proc Natl Acad Sci U S A. 2012 May 29;10

Insert

junctate (ASPH Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

77DAD79 to 77AAA79

Promoter

CMV

Available Since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only
Sox2-SE-RGM-eGFP targeting vector

Plasmid

#133045

Purpose

Targeting vector of RGM-eGFP with homologous arms around Sox2 super-enhancer DMR

Depositor

Rudolf Jaenisch

Article

Song et al Mol Cell. 2019 Aug 15. pii: S1097-2765(1

Insert

Sox2-SE-5arm-RGM-Sox2-SE-3arm

Use

Mouse Targeting

Available Since

Nov. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/TO PP1gamma-2*Strep

Plasmid

#113506

Purpose

Expresses dual Strep-tagged PP1gamma in mammalian cells

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

PP1gamma (PPP1CC Human)

Tags

Strep-Strep

Expression

Mammalian

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO.1P MUTYH_2

Plasmid

#160786

Purpose

Suppress MUTYH

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shMUTYH_2

Use

Lentiviral

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1P MUTYH_1

Plasmid

#160785

Purpose

Suppress MUTYH

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shMUTYH_1

Use

Lentiviral

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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p5065 pcDNA3.1/V5-His-TOPO hBrd4 (888-1362)

Plasmid

#14444

Depositor

Peter Howley

Article

You et al Cell. 2004 Apr 30. 117(3):349-60.

Insert

Brd4 (888-1362) (BRD4 Human)

Expression

Mammalian

Mutation

amino acids 888-1362

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
ArpC2_pLib

Plasmid

#173679

Purpose

ArpC2 subunit of the Human Arp2/3 complex in a library vector for the biGBac system of insect expression

Depositor

Roberto Dominguez

Article

Zimmet et al Sci Adv. 2020 Jun 5;6(23). pii: 6/23/eaa

Insert

ArpC2 (ARPC2 Human)

Expression

Insect

Promoter

polyhedrin

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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ADAP1

Plasmid

#163905

Purpose

Full length mouse ADAP1 for purification from insect cells

Depositor

Martin Loose

Article

Duellberg et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

Insert

ADAP1 (Adap1 Mouse)

Tags

Cleavable 6 x HIS tag

Expression

Insect

Promoter

polyhedrin

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pPN013

Plasmid

#91587

Purpose

Express sgRNA targeting human CSMD1

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

CSMD1 (CSMD1 Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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mRuby2-Alpha-Actinin-19

Plasmid

#55890

Purpose

Localization: Alpha Actinin, Excitation: 559, Emission: 600

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

Alpha-Actinin (ACTN1 Human)

Tags

mRuby2

Expression

Mammalian

Promoter

CMV

Available Since

March 12, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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NG1301 pCI-TPI-4MS2-SMG5(short)-4H

Plasmid

#65806

Purpose

NMD reporter mRNA

Depositor

Niels Gehring

Article

Boehm et al Cell Rep. 2014 Oct 23;9(2):555-68. doi:

Insert

TPI (TPI1 Human)

Tags

4 MS2 stem loops, SMG5 3'UTR shortened versi…

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only

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P63-sp64 Poly A

Plasmid

#59435

Purpose

synthetic poly A transcripts of VZV

Depositor

Randall Cohrs

Article

Cohrs et al J Virol. 2000 Dec;74(24):11464-71.

Insert

VZV ORF 63

Use

Makes synthetic transcripts that contains poly a

Tags

none

Mutation

none

Promoter

SP6

Available Since

Sept. 2, 2014

Availability

Academic Institutions and Nonprofits only
pCDNA3.1Xpress-Abr-HisC

Plasmid

#32509

Depositor

John Groffen, Nora Heisterkamp

Article

Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

Insert

ABR (ABR Human)

Tags

HISC

Expression

Mammalian

Available Since

Feb. 13, 2012

Availability

Academic Institutions and Nonprofits only
miR290-SE-RGM-eGFP targeting vector

Plasmid

#133046

Purpose

Targeting vector of RGM-eGFP with homologous arms around Mir290 super-enhancer DMR

Depositor

Rudolf Jaenisch

Article

Song et al Mol Cell. 2019 Aug 15. pii: S1097-2765(1

Insert

miR290-SE-5arm-RGM-miR290-SE-3arm

Use

Mouse Targeting

Expression

Mammalian

Available Since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

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mTFP1-Gamma-Tubulin-17

Plasmid

#55485

Purpose

Localization: Centrosomes, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

Gamma-Tubulin (TUBG1 Human)

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pSFG-TFII-I-GFP Y248&249F

Plasmid

#22196

Depositor

Ananda Roy

Article

Desgranges et al Mol Cell Biol. 2005 Dec . 25(24):10940-5

Insert

TFII-I (GTF2I Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Changed Tyrosines 248 and 249 to Phenylalanines. …

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only
pAG252

Plasmid

#33206

Depositor

David Bartel

Article

Garcia et al Nat Struct Mol Biol. 2011 Sep 11;18(10):

Insert

MAP4K4 3'UTR (MAP4K4 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Promoter

SV40 early enhancer/promoter

Available Since

Jan. 10, 2012

Availability

Academic Institutions and Nonprofits only
P29-sp64 Poly A

Plasmid

#59434

Purpose

synthetic poly A transcripts of VZV

Depositor

Randall Cohrs

Article

Cohrs et al J Virol. 2000 Dec;74(24):11464-71.

Insert

VZV ORF 29

Use

Makes synthetic transcripts that contains poly a

Tags

none

Mutation

none

Promoter

SP6

Available Since

Sept. 2, 2014

Availability

Academic Institutions and Nonprofits only
pGEX SH2D4B-SH2

Plasmid

#46482

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

SH2D4B (Sh2d4b Mouse)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 311-431

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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