We narrowed to 9,318 results for: control

1. pRDA_832

   Plasmid

   #216085

   Purpose

   Cas9 [Sp] knockout targeting CD47, positive control

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Insert

   CD47 guide
   

   Use

   CRISPR and Lentiviral; Positive controls

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRDB_166

   Plasmid

   #216093

   Purpose

   Cas9 [Sp] CRISPRi targeting CD55, positive control

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Insert

   CD55 guide
   

   Use

   CRISPR and Lentiviral; Positive controls

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBMN-HA-NAP1-L226Q/L233Q (delta-TBK1)

   Plasmid

   #208869

   Purpose

   For mammalian expression of NAP1 (delta-TBK1)

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al bioRxiv September 25, 202

   Insert

   NAP1 (AZI2 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   L226Q/L233Q (disrupts TBK1 binding)

   Available Since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX-4T1-MBP-TEV-NAP1_delta-NDP52 (S37K/A44E)

   Plasmid

   #208872

   Purpose

   For expression in E. coli of NAP1 (delta-NDP52)

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al bioRxiv September 25, 202

   Insert

   NAP1 (AZI2 Human)
   

   Tags

   MBP-TEV

   Expression

   Bacterial

   Mutation

   S37K/A44E (disrupts NDP52 binding)

   Available Since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX-4T1-MBP-TEV-NAP1_delta-TBK1 (L226Q/L233Q)

   Plasmid

   #208873

   Purpose

   For expression in E. coli of NAP1 (delta-TBK1)

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al bioRxiv September 25, 202

   Insert

   NAP1 (AZI2 Human)
   

   Tags

   MBP-TEV

   Expression

   Bacterial

   Mutation

   L226Q/L233Q (disrupts TBK1 binding)

   Available Since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pB-Neo-mCherry-mDYNLL1

   Plasmid

   #210336

   Purpose

   Overexpression of mDYNLL1

   Depositor

   Li Yu

   Article

   Jiao et al Cell. 2021 May 27;184(11):2896-2910.e13.

   Insert

   mDYNLL1 (Dynll1 Mouse)
   

   Tags

   mCherry

   Expression

   Mammalian

   Available Since

   Feb. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. MTOR-F2108L_DARIC(1)_CD19-scFv_AAV6_Donor

   Plasmid

   #211905

   Purpose

   Vector for AAV6 donor production. Targeted CD19-scFv DARIC transgene integration to the MTOR locus with the dominant rapamycin resistance MTOR-F2108L mutation via homology-directed repair.

   Depositor

   Yannick Doyon

   Article

   Levesque et al bioRxiv 2023.09.14.557485

   Insert

   DARIC-CD19-scFv
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   hPGK1

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. SVA-lncRNA AK057321 shRNA 1S scramble

   Plasmid

   #202837

   Purpose

   Lentiviral vector that expresses a scrambled control shRNA for SVA-lncRNA AK057321 shRNA 1S

   Depositor

   Matthew Anderson

   Article

   Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

   Insert

   SVA-lncRNA AK057321 shRNA 1S scramble control
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   6

   Available Since

   July 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. SVA-lncRNA AK057321 shRNA 2S scramble

   Plasmid

   #202839

   Purpose

   Lentiviral vector that expresses a scrambled control shRNA for SVA-lncRNA AK057321 shRNA 2S

   Depositor

   Matthew Anderson

   Article

   Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

   Insert

   SVA-lncRNA AK057321 shRNA 2S scramble control
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   July 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. Dr-FGFR1

    Plasmid

    #184005

    Purpose

    Dr-FGFR expression in mammalian cells

    Depositor

    Vladislav Verkhusha

    Article

    Leopold et al J Mol Biol. 2020 Jun 12;432(13):3749-376

    Insert

    Dr-FGFR1 (FGFR1 Human)
    

    Tags

    Deinococcus radiodurans bacterial phytochrome

    Expression

    Mammalian

    Available Since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pT2h70CRET(#325)

    Plasmid

    #184060

    Purpose

    cyclofen-inducible CRE activation in zebrafish transient transgenic

    Depositor

    Sophie Vriz

    Article

    Feng et al Sci Rep. 2017 Aug 23;7(1):9195. doi: 10.

    Insert

    CRE-ERT2
    

    Use

    Zebrafish transient transgenesis

    Mutation

    ERT2: G400V, M543A, L544A, deleted 1-281;

    Promoter

    Zf-Hsp70-4

    Available Since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJET-62-SFFV-T2A-PuroR-polyA-R11b

    Plasmid

    #179893

    Purpose

    Donor with homologous arms for Rab11b to knock in SFFV-T2A-Puromycin resistance-PolyA cassette (for knock out)

    Depositor

    Patrick Caswell

    Article

    Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

    Insert

    RAB11B, member RAS oncogene family (RAB11B Human)
    

    Use

    CRISPR; Donor for homologous based knock in

    Expression

    Bacterial and Mammalian

    Promoter

    SFFV

    Available Since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJET-64-SFFV-T2A-BlaR-polyA-R11b

    Plasmid

    #179895

    Purpose

    Donor with homologous arms for Rab11b to knock in SFFV-T2A-Blasticidin resistance-PolyA cassette (for knock out)

    Depositor

    Patrick Caswell

    Article

    Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

    Insert

    RAB11B, member RAS oncogene family (RAB11B Human)
    

    Use

    CRISPR; Donor for homologous based knock in

    Expression

    Bacterial and Mammalian

    Promoter

    SFFV

    Available Since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pmiRGLO Dual-Luciferase-PDAP1-3'UTR-Mut2x

    Plasmid

    #182254

    Purpose

    Human PDAP1 3` UTR region mutated in 2x binding site for miR-150 cloned downstream of luciferase reporter gene

    Depositor

    Silvia Monticelli

    Article

    Chirichella et al PLoS Biol. 2022 Feb 10;20(2):e3001538. d

    Insert

    3' UTR region of PDAP1 gene mutated in 2 binding sites for mir-150
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pmiRGLO Dual-Luciferase-PDAP1-3'UTR-Mut1x

    Plasmid

    #182253

    Purpose

    Human PDAP1 3` UTR region mutated in 1x binding site for miR-150 cloned downstream of luciferase reporter gene

    Depositor

    Silvia Monticelli

    Article

    Chirichella et al PLoS Biol. 2022 Feb 10;20(2):e3001538. d

    Insert

    3' UTR region of PDAP1 gene mutated in 1 binding site for mir-150
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pmiRGLO Dual-Luciferase-PDAP1-3'UTR-Mut3x

    Plasmid

    #182255

    Purpose

    Human PDAP1 3` UTR region mutated in 3x binding site for miR-150 cloned downstream of luciferase reporter gene

    Depositor

    Silvia Monticelli

    Article

    Chirichella et al PLoS Biol. 2022 Feb 10;20(2):e3001538. d

    Insert

    3' UTR region of PDAP1 gene mutated in 3 binding sites for mir-150
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBICI_high ori

    Plasmid

    #179273

    Purpose

    Expresses CuCBP and BaSP in E. coli BL21(DE3)

    Depositor

    Bernd Nidetzky

    Article

    Schwaiger et al Microb Cell Fact. 2022 Apr 9;21(1):61. d

    Inserts

    Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
    Cellobiose phosphorylase from Cellulomonas uda
    

    Use

    Synthetic Biology

    Tags

    His Tag and Strep - Tag II

    Expression

    Bacterial

    Promoter

    T7 lacO

    Available Since

    April 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDUBI

    Plasmid

    #179274

    Purpose

    Expresses CcCDP monocistronically and CuCBP and BaSP bicistronically

    Depositor

    Bernd Nidetzky

    Article

    Schwaiger et al Microb Cell Fact. 2022 Apr 9;21(1):61. d

    Inserts

    cellodextrin phosphorylase
    cellobiose phosphorylase
    C8077_RS00435 sucrose phosphorylase [ Bifidobacterium adolescentis (C8077_RS00435 Bifidobacterium alodescentis)
    

    Use

    Synthetic Biology

    Tags

    6xHIS and Strep-Tag II

    Expression

    Bacterial

    Mutation

    sucrose phosphorylase

    Promoter

    T7 lacO

    Available Since

    April 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDNA5-FRT-TO-KIF1A(1-365)-VVDfast-mVenus-SSPB(micro)_P2A_iLID-mCherry-RAB11

    Plasmid

    #174646

    Purpose

    Optogenetic coupling of RAB11 to opto-kinesin to induce anterograde transport of recycling endosomes. Compatible with Flp-in TREX system.

    Depositor

    Lukas Kapitein

    Article

    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    KIF1A(1-365)-VVDfast-mVenus-SSPB(micro)-P2A-iLID-mCherry-RAB11
    

    Expression

    Mammalian

    Mutation

    mmKIF1A(aa1-365): Pro202Ala; mVenus: Met1Del, Thr…

    Promoter

    CMV (with TetOn)

    Available Since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pB80-KIF1A(1-365)-mVenus-SSPB(micro)

    Plasmid

    #174635

    Purpose

    Non-processive monomeric KIF1A for optogenetic heterodimerization to iLID via SSPB(micro)

    Depositor

    Lukas Kapitein

    Article

    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    KIF1A(1-365)-mVenus-SSPB(micro) (Kif1a Mouse, Synthetic)
    

    Tags

    mVenus-SSPB(micro)

    Expression

    Mammalian

    Mutation

    mmKIF1A(aa1-365): Pro202Ala; mVenus: Met1Del, Thr…

    Promoter

    Chicken beta-actin

    Available Since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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