We narrowed to 6,183 results for: plasmid dna
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Plasmid#169562PurposeTier-1 vector carrying an internal ribosome entry site (IRES-pA)DepositorTypeEmpty backboneExpressionMammalianAvailable SinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only
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GAK gRNA (BRDN0001146687)
Plasmid#76470Purpose3rd generation lentiviral gRNA plasmid targeting human GAKDepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
GAK gRNA (BRDN0001146263)
Plasmid#76471Purpose3rd generation lentiviral gRNA plasmid targeting human GAKDepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
GAK gRNA (BRDN0001148048)
Plasmid#76472Purpose3rd generation lentiviral gRNA plasmid targeting human GAKDepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
p006kanGFP
Plasmid#58534PurposeGFP from jellyfish Aquorea sp. under T5 promoter with lac operators; kanamycin resistant.DepositorInsertGreen fluorescent protein
ExpressionBacterialPromoterT5 between two lac operatorsAvailable SinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
PB ID1-iCreERT2
Plasmid#139280PurposeHuman ID1-iCreERT2 transgene piggyBac transposonDepositorInsertID1 (ID1 Human)
UseCre/LoxExpressionMammalianMutationReplaced the ID1 protein coding sequence in the t…Available SinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-Puro (PX459) V2.0
Plasmid#62988PurposeCas9 from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)DepositorInserthSpCas9-2A-Puro V2.0
UseCRISPRTags2A-Puro and 3XFLAGExpressionMammalianPromoterCbhAvailable SinceMarch 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Tet-coBxb1-2A-BFP_IRES-iCasp9-2A-Blast_rtTA3
Plasmid#171588PurposeLentivector encoding a DNA recombinase landing pad with Bxb1 integrase and iCasp9 negative selection behind a Tet-inducible promoterDepositorInsertsBxb1 integrase
iCasp9
BFP
rtTA3G
UseLentiviral and Synthetic BiologyExpressionMammalianAvailable SinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ202
Plasmid#86198PurposeMultisite Gateway T-DNA entry plasmid (attR1 & attR2); AtUBQ10 promoter and Hygromycin resistance markerDepositorTypeEmpty backboneUseGateway compatible attr1-attr2 destination vectorExpressionPlantPromoterArabidopsis ubiquitin 10 (AtUbi10)Available SinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCA11-pMa-sgTS3-17xMS2V5-sgTS3
Plasmid#199442PurposeCRISPR donor plasmid to insert 17XMS2V5 into the ribosomal DNA region in dependent of homology armsDepositorInsertHITI donor template
UseCRISPRAvailable SinceMay 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pP2A_NLS_sfGFP
Plasmid#63709PurposeExpressing P2A_NLS_sfGFP with homologous arm of Nanog.DepositorInsertNanog homologous arm, p2A, NLS, sfGFP, Nanog homologous arm
UseJust a donor dna with nanog homologous arm for kn…TagssfGFPAvailable SinceApril 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
LLP783_SpyCatcher-DNMT3A-CO
Plasmid#211776PurposeSpyTag counterpart binding domain, SpyCatcher, fused to DNA methyltransferase DNMT3A, with GFP selectionDepositorInsertTraptavudin-DNMT3A
Tags3xV5ExpressionMammalianMutationLast 300 bp codon-optimised (CO) to detect DNMT3A…PromoterpEF1a and pSV40Available SinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
LLP784_SnoopCatcher-DNMT3A-CO
Plasmid#211777PurposeSnoopTag counterpart binding domain, SnoopCatcher, fused to DNA methyltransferase DNMT3A, with GFP selectionDepositorInsertSpy-EZH2
Tags3xFLAGExpressionMammalianMutationLast 300 bp codon-optimised (CO) to detect EZH2 e…PromoterpEF1a and pSV40Available SinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRpr150-LUC
Plasmid#85700Purposeluciferase reporter driven by a DNA damage responsive cis-regulatory sequence from the reaper locus, containing a p53 binding site (Rpr150 enhancer)DepositorInsertRpR 150 enhancer and hsp70 minimal promoter
UseLuciferaseTagsluciferaseExpressionMammalianPromoterRpR 150 enhancer and hsp70 minimal promoterAvailable SinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
LLP785_Traptavidin-DNMT3A-CO
Plasmid#211778PurposeAviTag counterpart binding domain, Traptavidin, fused to DNA methyltransferase DNMT3A, with GFP selectionDepositorInsertTraptavidin-EZH2
Tags3xV5ExpressionMammalianMutationLast 300 bp codon-optimised (CO) to detect EZH2 e…PromoterpEF1a and pSV40Available SinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
Gal4-HSF4b WT
Plasmid#118358PurposeThis plasmid expresses amino-acids 200-493 of hHSF4b WT fused to the GAL-4 DNA binding domain.DepositorAvailable SinceNov. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJK193
Plasmid#71834PurposeCloned region contains Acetobacter aceti 1023 rpmE-radC(and beyond) regionDepositorInsertsN5-carboxyaminoimidazole ribonucleotide mutase
N5-carboxyaminoimidazole ribonucleotide synthetase
UsePhage lambda cloning vector with genomic dna inse…Mutationadditional N-terminal methionine encoded by this …Available SinceJan. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pCJH027_SC101_pLAC_LbCas12a_Kan
Plasmid#183074PurposeWild-type LbCas12a (Chen et al., 2018) in a plasmid containing a SC101 origin and lac promoter for in vivo interference assay.DepositorInsertLbCas12a
ExpressionBacterialMutationWild-typeAvailable SinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only