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psigma2-EGFP

Plasmid

#53610

Purpose

Expresses sigma2 of adaptor protein of AP2

Depositor

Tom Kirchhausen

Article

Kural et al Cell Rep. 2012 Nov 29;2(5):1111-9. doi:

Insert

sigma2 (Ap2s1 Rat)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pET16b-His-ArgRS

Plasmid

#124104

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Argin+I4 ligase (argS Escherichia coli)

Tags

10x-His

Expression

Bacterial

Available Since

June 17, 2019

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP

Plasmid

#21181

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

Insert

enhanced green flourescent protein

Use

Drosophila expression

Available Since

June 23, 2009

Availability

Academic Institutions and Nonprofits only

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T7-CMVtrans-FFLuc-polyA

Plasmid

#101156

Purpose

Template for T7 RNAP production of RNA transcript for expression of firefly luciferase in a eukaryotic mammalian cell free lysate.

Depositor

Marcel Bruchez

Article

Canady et al J Am Chem Soc. 2015 Aug 19;137(32):10268

Insert

Firefly luciferase

Use

Luciferase

Promoter

T7 promoter

Available Since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-mGFP-Actin

Plasmid

#21948

Depositor

Ryohei Yasuda

Article

Murakoshi et al Brain Cell Biol. 2008 Aug . 36(1-4):31-4

Insert

betaActin (ACTB Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Sept. 3, 2009

Availability

Academic Institutions and Nonprofits only

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pAcBac1.tR4-MbPyl

Plasmid

#50832

Purpose

baculovirus-based delivery system that enables the efficient incorporation of unnatural amino acids into proteins in mammalian cells

Depositor

Peter Schultz

Article

Chatterjee et al Proc Natl Acad Sci U S A. 2013 Jul 16;11

Inserts

two-copy Pyl tRNA cassette
MbPyl
two copy Pyl tRNA cassette

Use

Baculoviral

Expression

Insect

Promoter

CMV and U6 and H1

Available Since

Feb. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pEB2-mScarlet

Plasmid

#104006

Purpose

Plasmid encoding mScarlet

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mScarlet

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to DsRed (MSKGE AVIKEF… N6A, R…

Promoter

proC

Available Since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HA Arf1 DN T31N

Plasmid

#10833

Depositor

Thomas Roberts

Article

Furman et al J Biol Chem. 2002 Mar 8. 277(10):7962-9.

Insert

Arf1 T31N (ARF1 Human)

Tags

HA

Expression

Mammalian

Mutation

T31N. Defective in GTP binding. Dominant negati…

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

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pMXs-3XFLAG-EGFP-OMP25

Plasmid

#83354

Purpose

For tagging mitochondria with Sigma FLAG epitopes

Depositor

David Sabatini

Article

Chen et al Cell. 2016 Aug 25;166(5):1324-1337.e11.

Insert

3XFLAG-EGFP-OMP25

Use

Retroviral

Expression

Mammalian

Available Since

Sept. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pKM201

Plasmid

#13078

Depositor

Kenan Murphy

Article

Murphy et al BMC Mol Biol. 2003 Dec 13;4:11. doi: 10.

Insert

Ptac-gam-bet-exo operon

Expression

Bacterial

Available Since

Oct. 7, 2006

Availability

Academic Institutions and Nonprofits only

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L4440gtwy

Plasmid

#11344

Depositor

Guy Caldwell

Insert

gateway cassette

Use

RNAi

Expression

Worm

Available Since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

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pmCherry-C1-RanQ69L

Plasmid

#30309

Depositor

Jay Brenman

Article

Kazgan et al Mol Biol Cell. 2010 Oct 1. 21(19):3433-4

Insert

RanQ69L (RAN N/A)

Tags

FLAG-Cherry

Expression

Mammalian

Mutation

Glutamine 69 is changed to Leucine

Available Since

July 7, 2011

Availability

Academic Institutions and Nonprofits only

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AAV-U6-dgRNA-CAG-MPH

Plasmid

#106259

Purpose

Expresses MPH co-activator from the CAG promoter and one U6-driven dgRNA in AAV backbone

Depositor

Juan Belmonte

Article

Liao et al Cell. 2017 Dec 14;171(7):1495-1507.e15.

Insert

MS2-P65-HSF1 (HSF1 Synthetic, Human)

Use

AAV and CRISPR

Expression

Mammalian

Promoter

CAG

Available Since

May 2, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-BE2

Plasmid

#73020

Purpose

Expresses BE2 in mammalian cells

Depositor

David Liu

Article

Komor et al Nature. 2016 Apr 20. doi: 10.1038/nature

Insert

BE2

Expression

Mammalian

Promoter

CMV

Available Since

April 14, 2016

Availability

Academic Institutions and Nonprofits only

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JDS246

Plasmid

#43861

Purpose

Expresses mammalian codon optimized Cas9 nuclease with C-term 3X FLAG from CMV and T7 promoters

Depositor

Keith Joung

Insert

mammalian codon-optimized streptococcus pyogenes Cas9 - 3X Flag

Use

CRISPR

Tags

3X FLAG

Expression

Mammalian

Promoter

CMV

Available Since

March 8, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO-ChRmine-mScarlet-WPRE

Plasmid

#130998

Purpose

Double floxed ChRmine-mScarlet under the control of Ef1a promoter

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Has Service

AAV1 and AAV5

Insert

ChRmine

Use

AAV

Tags

mScarlet

Promoter

Ef1a

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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scFv-sfGFP-DNMT3A1

Plasmid

#102278

Purpose

The plasmid encodes a single-chain antibody that binds to the GCN4 peptide from the SunTag system, and is fused to a DNA methyltransferase DNMT3A1

Depositor

Margaret Goodell

Article

Huang et al Genome Biol. 2017 Sep 18;18(1):176. doi:

Insert

DNMT3A1 (DNMT3A Human)

Use

Lentiviral

Tags

scFV-sfGFP

Expression

Mammalian

Available Since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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Hygro-Cas9 donor

Plasmid

#86883

Purpose

Donor vector for genomic targeting of a Tetracycline-inducible Cas9 cassette to the human AAVS1/PPP1R12C locus with hygromycin selection

Depositor

Danwei Huangfu

Article

Shi et al Cell Stem Cell. 2017 Feb 8. pii: S1934-5

Insert

hSpCas9

Use

CRISPR

Tags

3xFlag, Nucleoplasmin NLS, and SV40 NLS

Expression

Mammalian

Available Since

March 2, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti6-DEST PINK1-V5 WT

Plasmid

#13320

Depositor

Mark Cookson

Article

Beilina et al Proc Natl Acad Sci U S A. 2005 Apr 19. 1

Insert

PINK1 (PINK1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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pT7-NSP1SA11

Plasmid

#89168

Purpose

Express Rotavirus SA11 NSP1 in mammalian cells

Depositor

Takeshi Kobayashi

Article

Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

Insert

Rotavirus SA11 NSP1

Expression

Mammalian

Promoter

T7 promotor

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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