We narrowed to 11,735 results for: pcdna

1. pcDNA5/FRT/TO GFP HSPH3

   Plasmid

   #19482

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

   Insert

   HSPH3 (HSPA4L Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 7, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-Ha-Brca1 S1423A/S1524A

   Plasmid

   #32539

   Depositor

   Michael Kastan

   Article

   Xu et al Cancer Res. 2002 Aug 15;62(16):4588-91.

   Insert

   BRCA1 (BRCA1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S1423A/S1524A

   Promoter

   CMV/rabbit B-Globin

   Available since

   Oct. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

3. FLAG-KLF12/pcDNA3.1-Neo

   Plasmid

   #81069

   Purpose

   Expression of epitope-tagged human KLF12 in mammalian cells.

   Depositor

   Julian Downward

   Article

   Godin-Heymann et al Oncogene. 2016 Jun 23;35(25):3324-34. do

   Insert

   FLAG-KLF12 (KLF12 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3.1(+)Gα15-244-NLuc

   Plasmid

   #207154

   Purpose

   Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNA15 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

   Depositor

   Dominique Schols, Tom Van Loy

   Article

   Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

   Insert

   GNA15 (GNA15 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   NLuc inserted at position E(244)N

   Promoter

   Available since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3 c-SRC (250-536)

   Plasmid

   #42208

   Depositor

   Robert Lefkowitz

   Article

   Miller et al J Biol Chem. 2000 Apr 14;275(15):11312-9

   Insert

   c-SRC (250-536) (SRC Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   contains amino acids 250-536

   Promoter

   CMV

   Available since

   Feb. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

6. IRE1b(K536A).9E10.pCDNA3

   Plasmid

   #21881

   Depositor

   David Ron

   Insert

   IRE1b (Ern2 Mouse)
   

   Use

   Tags

   Myc (9E10)

   Expression

   Mammalian

   Mutation

   K536A

   Promoter

   Available since

   Nov. 10, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. GFP Cav2.2 HA D122A pcDNA3

   Plasmid

   #206120

   Purpose

   expression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and an exofacial double HA tag in domain II and a D122A mutation that disrupts the interaction with ?2?-1

   Depositor

   Annette Dolphin

   Article

   Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

   Insert

   cacna1b (CACNA1B Rabbit)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D122A

   Promoter

   CMV

   Available since

   Nov. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Cav2.2 HA E1658A (EIVA) pcDNA3

   Plasmid

   #206114

   Purpose

   expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and an E1658A mutation in the P loop of Domain IV which reduces trafficking to the cell surface

   Depositor

   Annette Dolphin

   Article

   Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

   Insert

   cacna1b (CACNA1B Rabbit)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E1658A

   Promoter

   CMV

   Available since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Cav2.1 EIVA (E1707A) pcDNA3

   Plasmid

   #206125

   Purpose

   expression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and a mutation in the P loop of Domain IV to to abolish divalent cation binding

   Depositor

   Annette Dolphin

   Article

   Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

   Insert

   cacna1a (Cacna1a Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E1707A

   Promoter

   CMV

   Available since

   Oct. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Cav2.1 HA EIA (E320A) pcDNA3

    Plasmid

    #206124

    Purpose

    expression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and a mutation in the P loop of Domain I to abolish divalent cation binding

    Depositor

    Annette Dolphin

    Article

    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

    Insert

    cacna1a (Cacna1a Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E320A

    Promoter

    CMV

    Available since

    Oct. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3-HA-REDD1 77-232 (#426)

    Plasmid

    #99921

    Purpose

    mammalian expression

    Depositor

    James Brugarolas

    Article

    Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

    Insert

    REDD1 (DDIT4 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    N-terminal deletion; encodes AA 77-232

    Promoter

    Available since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA5/FRT/TO GFP DNAJB5

    Plasmid

    #19500

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

    Insert

    DNAJB5 (DNAJB5 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.1-deltaA1-Lock-VWF

    Plasmid

    #124824

    Purpose

    Expresses multimeric human VWF protein without A1 domain and Lock A2 domain. This protein has C-terminal c-Myc tag.

    Depositor

    Sriram Neelamegham

    Article

    Zhang et al Blood Adv. 2019 Apr 9;3(7):957-968. doi:

    Insert

    VWF without A1 domain and Lock A2 domain (VWF Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3 WT MYOC eGLuc2 FLAG

    Plasmid

    #192820

    Purpose

    Expresses wild-type myocilin with a C-terminal enhanced Gaussia luciferase and FLAG tag

    Depositor

    John Hulleman

    Article

    Nakahara et al Curr Eye Res. 2022 Jun;47(6):918-922. do

    Insert

    myocilin (MYOC Human)
    

    Use

    Tags

    FLAG and enhanced Gaussia luciferase (M43I/M110I)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA5/FRT/TO HIS DNAJB9

    Plasmid

    #19559

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

    Insert

    DnaJ (Hsp40) homolog, subfamily B, member 9 (DNAJB9 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA5-hygro-eGFP-HEC1

    Plasmid

    #114027

    Purpose

    Inducible expression of eGFP-tagged HEC1 in FLPin Trex cell lines

    Depositor

    Geert Kops

    Article

    Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

    Insert

    eGFP-HEC1 - Wildtype (NDC80) (NDC80 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3-FLAG-LplA(AAG)

    Plasmid

    #61307

    Purpose

    Contains FLAG epitope for immunofluorescence detection of resorufin ligase for mammalian cell expression

    Depositor

    Alice Ting

    Article

    Liu et al Proc Natl Acad Sci U S A. 2014 Oct 28;11

    Insert

    E. coli lipoic acid ligase
    

    Use

    Tags

    6xHis, Flag, T7 tag, and Xpress epitope

    Expression

    Mammalian

    Mutation

    E20A, F147A, H149G

    Promoter

    CMV

    Available since

    March 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3-PKCsub-YPet-NES

    Plasmid

    #138207

    Purpose

    Encodes C-terminal (substrate) fragment of FRET-based bimolecular PKC activity reporter (bimCKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES

    Depositor

    Jin Zhang

    Article

    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

    Insert

    PKCsub-YPet-NES
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCDNA5/FRT_CMV_NES-Caprola_06-mEGFP

    Plasmid

    #194684

    Purpose

    CMV driven expression of the calcium recorder Caprola_06 in mammalian cell lines fused to mEGFP

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_06-mEGFP
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCDNA3-human C-MYC-V394D

    Plasmid

    #74162

    Purpose

    Mammalian expression of human cMyc VD mutant

    Depositor

    Martine Roussel

    Article

    Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

    Insert

    cMyc (MYC Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    V394D

    Promoter

    CMV

    Available since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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