159,589 results

1. WT GLUT1-FLAG

   Plasmid

   #200094

   Purpose

   WT human GLUT1 with a C-terminal 3xFLAG tag in pQCXIP vector

   Depositor

   Jason MacGurn

   Article

   Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

   Insert

   GLUT1 (SLC2A1 Human)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. CMV-Hylight-T152E

   Plasmid

   #193448

   Purpose

   A binding-dead fluorescent biosensor for fructose 1,6-bisphosphate (FBP) for negative control experiments (mammalian expression vector)

   Depositor

   Richard Goodman

   Article

   Koberstein et al Proc Natl Acad Sci U S A. 2022 Aug 2;119

   Insert

   Binding-Dead Hylight
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Threonine 67 (ACT) mutated to a Glutamic Acid (GA…

   Promoter

   CMV

   Available since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. Fucci(CA) hCdt1-iRFP hGeminin-TagBFP2

   Plasmid

   #190181

   Purpose

   Fluorescent reporter vector to visualize all of cell cycle phases.

   Depositor

   Toshiro Sato

   Article

   Ohta et al Nature. 2022 Jul 7. pii: 10.1038/s41586-

   Inserts

   iRFP
   hCdt1(1/100)Cy(-) (Cdt1 Synthetic)
   TagBFP2
   hGeminin(1/110)
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3puro-mGFP-FMR1 isoform1-sh1Resistant

   Plasmid

   #225462

   Purpose

   Express mEGFP-fusion protein of FMR1 isoform 1

   Depositor

   Christine Mayr

   Article

   Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

   Insert

   FMR1 isoform 1 (FMR1 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJMP1339

   Plasmid

   #119271

   Purpose

   for cloning new sgRNAs

   Depositor

   Carol Gross, Jason Peters, Oren Rosenberg

   Article

   Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

   Insert

   dcas9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D10A and H840A

   Promoter

   Available since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti6MFV SLC10A1

   Plasmid

   #194130

   Purpose

   Expresses NTCP (SLC10A1) protein in mammalian cells

   Depositor

   Jun Liu

   Insert

   SLC10A1 (SLC10A1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. MSCV IRES Luciferase

   Plasmid

   #18760

   Depositor

   Scott Lowe

   Type

   Empty backbone

   Use

   Luciferase and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 3, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFB2A

   Plasmid

   #195283

   Purpose

   Bicistronic DTR and Hygromycin resistance for positive/negative selection

   Depositor

   Scott Lowe

   Article

   Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

   Insert

   DTR 2A Hygro (HBEGF Synthetic, Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   Jan. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRK172-6xHis-0N4R-P301S-tau-HiBiT

   Plasmid

   #187835

   Purpose

   Expression of 6xHis-tagged P301S tau of 0N4R isoform with a C-terminal HiBiT tag for bimolecular luminescence with LgBiT.

   Depositor

   William McEwan

   Article

   Tuck et al Cell Rep. 2022 May 3;39(5):110776. doi:

   Insert

   6XHis-0N4R-P301S-tau-HiBiT
   

   Use

   Tags

   6xHistidine and HiBiT

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. Lenti-multi-CRISPR

    Plasmid

    #85402

    Purpose

    Lentiviral vector for the delivery of multiple sgRNAs targeting different genes with constitutive Cas9 expression

    Depositor

    Qin Yan

    Article

    Cao et al Nucleic Acids Res. 2016 Nov 2;44(19):e14

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYD1

    Plasmid

    #73447

    Purpose

    expression vector designed for expression, secretion, and display of proteins on the extracellular surface of Saccharomyces cerevisiae cells

    Depositor

    Dane Wittrup

    Article

    Kieke et al Protein Eng. 1997 Nov;10(11):1303-10.

    Type

    Empty backbone

    Use

    Tags

    6xHis, AGA2, T7 peptide, V5 tag, and Xpress tag

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDONR221_SLC1A2

    Plasmid

    #131901

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC1A2 (SLC1A2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pCap-101-Hgy

    Plasmid

    #227508

    Purpose

    Capture plasmid with hygromycin resistance and pIJ101 replicon

    Depositor

    Chengzhang Fu

    Article

    Xie et al Science. 2024 Dec 13;386(6727):eabq7333.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-hSyn-EGFP

    Plasmid

    #50465

    Purpose

    EGFP under the control of human synapsin promoter

    Depositor

    Bryan Roth

    Has Service

    AAV PHP.eB, AAV Retrograde, AAV Retrograde trial size, AAV1, AAV1 trial size, AAV2, AAV2 trial size, AAV5, AAV5 trial size, AAV8, AAV8 trial size, AAV9, and AAV9 trial size

    Insert

    EGFP
    

    Use

    AAV

    Tags

    N/A

    Expression

    Mutation

    Promoter

    human Synapsin 1

    Available since

    March 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. VCP(wt)-EGFP

    Plasmid

    #23971

    Depositor

    Nico Dantuma

    Article

    Tresse et al Autophagy. 2010 Feb 22. 6(2):.

    Insert

    Valosin containing protein (VCP Human)
    

    Use

    Tags

    6xHis, EGFP, and HA

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Available since

    Feb. 26, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSpCas9(BB)-2A-Blast

    Plasmid

    #118055

    Purpose

    Used for expression of sgRNA in mammalian cells

    Depositor

    Ken-Ichi Takemaru

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGDP1 NDM-1

    Plasmid

    #112883

    Purpose

    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

    Depositor

    Gerard Wright

    Article

    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

    Insert

    NDM-1 (blaNDM-1 Klebsiella pneumoniae)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Pbla

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMito-iRFP670

    Plasmid

    #45462

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

    Insert

    iRFP670
    

    Use

    Tags

    human cytochrome C oxidase subunit VIII

    Expression

    Mammalian

    Mutation

    T2A, S45T, V116I, D124E, M170L, K180M, D202L, I20…

    Promoter

    CMV

    Available since

    June 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBJ FLAG-hPar1

    Plasmid

    #53226

    Purpose

    mammalian expression of human Par1 with N-terminal FLAG tag

    Depositor

    Shaun Coughlin

    Article

    Ishii et al J Biol Chem. 1993 May 5. 268(13):9780-6.

    Insert

    Par1 (F2R Human)
    

    Use

    Tags

    FLAG and bovine prolactin signal sequence (MDSKGS…

    Expression

    Mammalian

    Mutation

    Deletion of aa1-39 (native signal peptide)

    Promoter

    SV40

    Available since

    Aug. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRel-Hyg

    Plasmid

    #227582

    Purpose

    Release plasmid with Cas9 working cassette, hygromycin resistance, and pSG5 replicon

    Depositor

    Chengzhang Fu

    Article

    Xie et al Science. 2024 Dec 13;386(6727):eabq7333.

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge