We narrowed to 18,341 results for: met

1. pAAV-EF1α1.1-FLPX-rc [Chronos-GFP]

   Plasmid

   #122102

   Purpose

   AAV-mediated expression of Chronos-GFP under the EF1α1.1 promoter in frt/reversed (Flp-dependent) manner.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   Chronos-GFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a（1.1kb short version)

   Available Since

   Sept. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pRSFDuet-1_VNp-LZ_VNp-LZ-mNeongreen-hGH

   Plasmid

   #182425

   Purpose

   Bacterial expression of Vesicle Nucleating peptide-Leucine Zipper and Vesicle Nucleating peptide-Leucine Zipper-mNeongreen-human growth hormone fusion proteins

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Inserts

   VNp-LZ-mNeongreen-hGH (GH1 Human)
   VNp-LZ
   

   Use

   Tags

   Vesicle Nucleating peptide (VNp) and Vesicle Nucl…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. mIFP24-LaminA-C-18

   Plasmid

   #56267

   Purpose

   Localization: Nuclear Envelope, Excitation: 683, Emission: 704

   Depositor

   Michael Davidson

   Article

   Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

   Insert

   LaminA (LMNA Human)
   

   Use

   Tags

   mIFP24

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 28, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

4. LMP AMPK alpha2 hp1

   Plasmid

   #89490

   Purpose

   shRNA against AMPK alpha2 in mouse/human to knock down protein expression

   Depositor

   Russell Jones

   Article

   Faubert et al Cell Metab. 2013 Jan 8;17(1):113-24. doi

   Insert

   hairpin against mouse/human AMPKalpha2 (PRKAA2, Prkaa2 )
   

   Use

   Mouse Targeting and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   LMP

   Available Since

   May 19, 2017

   Availability

   Academic Institutions and Nonprofits only

5. Fgf4enhLV (FpG12)

   Plasmid

   #69444

   Purpose

   positive control plasmid for lentiviral reporter expression in ESCs

   Depositor

   Lisa Dailey

   Article

   Murtha et al Nat Methods. 2014 May;11(5):559-65. doi:

   Inserts

   minimal FGF4 promoter
   EGFP
   Ubiquitin promoter
   Hygromycin resistance
   FGF4 enhancer (Fgf4 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)

   Plasmid

   #129028

   Purpose

   inactivated control (targeted DNA demethylation),expression of dCas9-hudTET1CD-T2A-mCherry and cloning backbone for sgRNA

   Depositor

   Julia K Polansky

   Article

   Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

   Insert

   dCas9-hudTET1CD, SgRNA cloning site
   

   Use

   Tags

   HA-Tag, NLS and T2A-mCherry

   Expression

   Mammalian

   Mutation

   dCas9 (D10A;H840A), catalytic domain huTET1 inact…

   Promoter

   CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

   Available Since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-P3-Alb-Rosmarinus

   Plasmid

   #183462

   Purpose

   To produce AAV with the P3 promoter to express Alb(mouse)-rosmarinus fusion protein

   Depositor

   Hajime Hirase

   Article

   Wang et al Cell Rep Methods. 2022 Sep 21;2(10):1003

   Insert

   Alb (Alb Mouse)
   

   Use

   AAV

   Tags

   Rosmarinus

   Expression

   Mutation

   Promoter

   Available Since

   May 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. mIFP-LaminA-C-18

   Plasmid

   #56226

   Purpose

   Localization: Nuclear Envelope, Excitation: 683, Emission: 704

   Depositor

   Michael Davidson

   Article

   Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

   Insert

   LaminA (LMNA Human)
   

   Use

   Tags

   mIFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 28, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

9. pAAV-P3-Alb-mCarmine

   Plasmid

   #183464

   Purpose

   To produce AAV with the P3 promoter to express Alb-mCarmine fusion protein

   Depositor

   Hajime Hirase

   Article

   Wang et al Cell Rep Methods. 2022 Sep 21;2(10):1003

   Insert

   Alb (Alb Mouse)
   

   Use

   AAV

   Tags

   mCarmine

   Expression

   Mutation

   Promoter

   Available Since

   May 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. FLINC-AKAR1(TA)-CAAX

    Plasmid

    #87706

    Purpose

    Negative-control mutant of FLINC-AKAR1-CAAX.

    Depositor

    Jin Zhang

    Article

    Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi

    Insert

    FLINC-AKAR1(TA)-CAAX
    

    Use

    Tags

    6xHis, C-terminal targeting sequence from Kras, T…

    Expression

    Mammalian

    Mutation

    Target Thr residue in substrate domain mutated to…

    Promoter

    CMV

    Available Since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. PKAsub-TagRFPT-CAAX

    Plasmid

    #87712

    Purpose

    Encodes C-terminal (substrate) fragment of bimolecular super-resolution PKA activity reporter (bimFLINC-AKAR); targeted to plasma membrane; use in conjunction with Dronpa-FHA1 (Addgene #87711).

    Depositor

    Jin Zhang

    Article

    Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi

    Insert

    PKAsub-TagRFPT-CAAX
    

    Use

    Tags

    6xHis, C-terminal targeting sequence from Kras, T…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pOTTC351 - pAAV CaMKIIa hChR2(H134R)-iRFP

    Plasmid

    #47905

    Purpose

    An AAV vector that expresses channel rhodopsin 2 (H134R) (fused to iRFP) under the CaM Kinase IIa promoter.

    Depositor

    Brandon Harvey

    Article

    Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

    Insert

    hChR2(H134R)
    

    Use

    AAV

    Tags

    iRFP

    Expression

    Mammalian

    Mutation

    H134R

    Promoter

    CaMKIIa

    Available Since

    Sept. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. mIFP12-Farnesyl-5

    Plasmid

    #56251

    Purpose

    Localization: Plasma Membrane, Excitation: 683, Emission: 704

    Depositor

    Michael Davidson

    Article

    Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

    Insert

    Farnesyl (HRAS Human)
    

    Use

    Tags

    mIFP12

    Expression

    Mammalian

    Mutation

    encodes final 20 aa of NM_001130442.1

    Promoter

    CMV

    Available Since

    April 28, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. mIFP12-FilaminA-C-14

    Plasmid

    #56253

    Purpose

    Localization: Actin/Focal Adhesions, Excitation: 683, Emission: 704

    Depositor

    Michael Davidson

    Article

    Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

    Insert

    FilaminA (FLNA Human)
    

    Use

    Tags

    mIFP12

    Expression

    Mammalian

    Mutation

    D2626H

    Promoter

    CMV

    Available Since

    April 28, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. mIFP12-LaminA-C-18

    Plasmid

    #56256

    Purpose

    Localization: Nuclear Envelope, Excitation: 683, Emission: 704

    Depositor

    Michael Davidson

    Article

    Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

    Insert

    LaminA (LMNA Human)
    

    Use

    Tags

    mIFP12

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 28, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

16. pAAV-CAG-NLS-DNMT3A-L-N-spC9-N-intein-hGH

    Plasmid

    #112212

    Purpose

    Targeted DNA methylation

    Depositor

    Xingxu Huang

    Article

    Lu et al Cell Death Dis. 2020 Feb 3;11(2):85. doi

    Insert

    N-terminus of dCas9 and DNMT3A (DNMT3A Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    H840A,N863A

    Promoter

    Available Since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCSDest2-SpCas9-NLS-3XHA-NLS-Zif268

    Plasmid

    #69219

    Purpose

    Expresses wild type SpCas9 fused to Zif268 in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    Zif268 (Egr1 Mouse)
    

    Use

    CRISPR

    Tags

    NLS-3XHA-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. JA412

    Plasmid

    #49935

    Purpose

    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human KLF4 gene and demethylate CpGs adjacent to the binding site

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    Tet1CD KL-1 (KLF4 Human)
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Dec. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pAAV-EF1α1.1-Chronos-GFP

    Plasmid

    #122098

    Purpose

    AAV-mediated expression of Chronos-GFP under the EF1α promoter (1.1kb short version). Using SV40 pA signal.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    Chronos-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α (1.1 kb short version)

    Available Since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. SL357

    Plasmid

    #49936

    Purpose

    Expresses a TALE-TET1FL (full length TET1) fusion protein engineered to bind a site in the human KLF4 gene and demethylate CpGs adjacent to the binding site

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    Tet1FL KL-1 (KLF4 Human)
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Dec. 20, 2013

    Availability

    Academic Institutions and Nonprofits only