We narrowed to 17,632 results for: URE

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pEGFP-mNFM

Plasmid

#32909

Purpose

Mammalian expression construct driving the expression of a fluorescent fusion protein consisting of EGFP linked to the N-terminus of mouse neurofilament protein M

Depositor

Anthony Brown

Article

Yan et al Cell Motil Cytoskeleton. 2007 Apr;64(4):

Insert

EGFP-mouse NFM fusion (Nefm Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pCDH_Str-KDEL_neomycin

Plasmid

#65307

Purpose

Luminal ER hook only (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Streptavidin fused to a signal sequence at 5' end and to a KDEL motif at 3' end

Use

Lentiviral

Tags

KDEL motif and Signal Sequence

Promoter

CMV

Available Since

Aug. 11, 2015

Availability

Academic Institutions and Nonprofits only

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His6_MBP_FXR1P

Plasmid

#198694

Purpose

Expression of human FXR1 protein in E. coli

Depositor

Simpson Joseph

Article

Edwards et al Sci Rep. 2020 Sep 28;10(1):15858. doi: 1

Insert

FXR1P (FXR1 Human)

Tags

MBP

Expression

Bacterial

Promoter

T7

Available Since

May 18, 2023

Availability

Academic Institutions and Nonprofits only

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NbV5-HALOTAG

Plasmid

#201482

Purpose

Indirect detection of V5-tagged protein using the HaloTag protein labeling technology.

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

NbV5-HALOTAG

Tags

HaloTag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_flGalT-SBP-EGFP

Plasmid

#65272

Purpose

synchronize trafficking of GalT from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

galactosidase

Tags

EGFP and Streptavidin Binding Protein (SBP)

Expression

Mammalian

Promoter

CMV

Available Since

June 19, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-Vgf-T2A-mCherry-CW3SL

Plasmid

#223661

Purpose

Expresses VGF and mCherry in a Cre-dependent manner

Depositor

Oscar Marin

Article

Selten et al Nature. 2025 Apr 30. doi: 10.1038/s41586

Insert

Vgf-T2A-mCherry

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-hM3D(Gq)-mCherry-shVgf-CWB

Plasmid

#223658

Purpose

Expresses hM3D(Gq) and a shRNA targetting VGF in a Cre-dependent manner

Depositor

Oscar Marin

Article

Selten et al Nature. 2025 Apr 30. doi: 10.1038/s41586

Insert

hM3D(Gq)-mCherry and shVGF

Use

AAV and RNAi

Tags

mCherry

Expression

Mammalian

Promoter

hSyn

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pCDH-anti-mCherry(LaM6) PAGER(Gq)

Plasmid

#230016

Purpose

Lentiviral vector expressing anti-mCherry PAGER(Gq) in mammalian cells

Depositor

Alice Ting

Article

Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

Insert

MT1-LaM6-TEVcs-ALFA-hM1D

Use

Lentiviral

Expression

Mammalian

Mutation

W442A on hM1D

Promoter

CMV

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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YFP-NavII-III

Plasmid

#26056

Depositor

Matthew Grubb

Article

Grubb et al Nature. 2010 Jun 24. 465(7301):1070-4.

Insert

Nav1.2 (Scn2a Rat)

Tags

YFP

Expression

Mammalian

Mutation

The insert codes only for the intracellular loop …

Available Since

Oct. 4, 2010

Availability

Academic Institutions and Nonprofits only

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Lepr peak reporter-eGFP

Plasmid

#196584

Purpose

Cis-regulatory region of mouse Lepr gene driven a eGFP reporter with hGFP driven rtTA3

Depositor

Elaine Fuchs

Article

Yuan et al Nature. 2022 Nov 30. doi: 10.1038/s41586

Insert

eGFP

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

SV40

Available Since

Oct. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pBR322-Pdp1_NTD-Cre-HiBiT

Plasmid

#198318

Purpose

Loads HiBiT-tagged Cre into PVCs using Pdp1 packaging domain; also contains Pnf

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

Pdp1_NTD-Cre-HiBiT

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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ITPKA-mNeonGreen

Plasmid

#98883

Purpose

In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

ITPKA

Tags

mNeonGreen

Expression

Mammalian

Promoter

CMV

Available Since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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pBabepuro-HA-GASC1

Plasmid

#24182

Purpose

Mammalian expression of human JMJD2C with HA tag

Depositor

Kristian Helin

Article

Cloos et al Nature. 2006 Jul 20. 442(7100):307-11.

Insert

GASC1 (KDM4C Human)

Tags

HA

Expression

Mammalian

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
Arp2/3_pBIG2abcd

Plasmid

#173683

Purpose

Insect expression of the 7-subunit Human Arp2/3 complex

Depositor

Roberto Dominguez

Article

Zimmet et al Sci Adv. 2020 Jun 5;6(23). pii: 6/23/eaa

Inserts

Arp2
Arp3
ArpC2
ArpC3-FLAG
ArpC4
ArpC5
ArpC1

Expression

Insect

Promoter

polyhedrin

Available Since

Sept. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pMXs-c-Myc-IP

Plasmid

#15921

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

myelocytomatosis oncogene (Myc Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

None

Available Since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only

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pNP1047

Plasmid

#222726

Purpose

pNP555 - IS621 RE-LE TBL-T5, DBL-WT bRNA in cis

Depositor

Patrick Hsu

Article

Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

Insert

IS621 bridge RNA

Tags

sfGFP

Expression

Bacterial

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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B23(9-122)

Plasmid

#23142

Depositor

SeWon Suh

Article

Lee et al Proteins. 2007 Nov 15. 69(3):672-8.

Insert

B23 core (NPM1 Human)

Tags

His

Expression

Bacterial

Mutation

deleted amino acids 1-8

Available Since

Sept. 16, 2010

Availability

Academic Institutions and Nonprofits only
pBEL1092

Plasmid

#195635

Purpose

Expression/purification of 6xHis-TEV-sfGFP

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

sfGFP

Expression

Bacterial

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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enz.099-PKA

Plasmid

#154048

Purpose

Expresses PKA in E. coli

Depositor

Christopher Voigt

Article

Wallace et al Advanced Functional Materials 2020

Insert

Protein kinase cAMP-activated catalytic subunit alpha (PKA) (PRKACA Human)

Tags

His, MBP

Expression

Bacterial

Promoter

T7

Available Since

Dec. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P13

Plasmid

#120024

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mCherry

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mCherry

Use

Pcr template

Tags

mCherry

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMCRi

Plasmid

#207524

Purpose

Plasmid for CRISPRi; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→ Sp dCas9, PEM7 →sgRNA; SmR/SpR

Depositor

Pablo Ivan Nikel

Article

Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.

Insert

xylS (cured of BsaI-sites), Pm→ Sp dCas9, PEM7 →sgRNA; SmR/SpR

Use

Pseudomonas vector

Available Since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pAL101

Plasmid

#22474

Purpose

Expresses APB domain of PIF6 fused to mYFP, for red-light-induced binding to Phy

Depositor

Christopher Voigt

Article

Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

Insert

PIF6APB

Use

Synthetic Biology

Tags

mYFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 25, 2010

Availability

Academic Institutions and Nonprofits only

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pAAV-anti-mCherry(LaM6) PAGER(G12)

Plasmid

#230013

Purpose

Expresses anti-mCherry PAGER(G12) in mammalian cells

Depositor

Alice Ting

Article

Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

Insert

MT1-LaM6-TEVcs-ALFA-hM1Dx12

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-anti-mCherry(LaM6) PAGER(Gq)

Plasmid

#230010

Purpose

Expresses anti-mCherry PAGER(Gq) in mammalian cells

Depositor

Alice Ting

Article

Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

Insert

MT1-LaM6-TEVcs-ALFA-hM1D

Use

AAV

Expression

Mammalian

Mutation

W442A on hM1D

Promoter

CMV

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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flex-ChrimsonR-EYFP-Kv

Plasmid

#135319

Purpose

Cre-dependent expression of soma-targeted ChrimsonR

Depositor

Hongkui Zeng

Article

Hage et al Elife. 2022 Jan 21;11:e71103. doi: 10.75

Insert

ChrimsonR

Use

AAV

Tags

EYFP

Promoter

synapsin

Available Since

Jan. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pJAM1.21_PB-UGCG-PGK-Blast

Plasmid

#205566

Purpose

Express human UGCG

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

UGCG (UGCG Human)

Use

Piggybac

Expression

Mammalian

Mutation

WT

Promoter

EF1

Available Since

Sept. 19, 2023

Availability

Academic Institutions and Nonprofits only

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4xmt-mTurquoise2

Plasmid

#98819

Purpose

In vivo visualization of mitochondria (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

mts

Tags

mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pRN3P_membrane_EGFP

Plasmid

#139402

Purpose

in vitro transcription for mRNA

Depositor

Maria-Elena Torres-Padilla

Article

Borsos et al Nature. 2019 May;569(7758):729-733. doi:

Insert

membrane-EGFP

Use

In vitro transcription

Tags

EGFP

Promoter

T3

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pHR-flag-GFP-Intb1

Plasmid

#205193

Purpose

This vector encodes of a synCAM (Intb1) and GFP

Depositor

Wendell Lim

Article

Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

Insert

synCAM Intb1 and GFP

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-anti-TNFalpha(Ozoralizumab) PAGER(FL)

Plasmid

#230024

Purpose

Expresses anti-TNFalpha PAGER(FL) in mammalian cells

Depositor

Alice Ting

Article

Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

Insert

pAAV-MT3-Ozoralizumab-TEVcs-ALFA-hM4DxcpGFP

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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