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We narrowed to 156,048 results for: ins

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Showing: 7021 - 7050 of 156048 results

  1. pETM33_N_CTD

    Plasmid
    #156484
    Purpose
    Bacterial expression of Sars-CoV2 N_CTD protein with His-tag and GST-tag
    Depositor
    Ylva Ivarsson
    Article
    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:
    Insert
    N_CTD (N Synthetic, Severe acute respiratory syndrome coronavirus 2)
    Tags
    His-GST
    Expression
    Bacterial
    Mutation
    Gene insert is codon optimized for Ecoli.
    Promoter
    T7/LacO
    Available Since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    156484-plasmid-map-sequence-id-307735

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  2. pETM33_N_NTD

    Plasmid
    #156483
    Purpose
    Bacterial expression of Sars-CoV2 N_NTD protein with His-tag and GST-tag
    Depositor
    Ylva Ivarsson
    Article
    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:
    Insert
    N_NTD (N Synthetic, Severe acute respiratory syndrome coronavirus 2)
    Tags
    His-GST
    Expression
    Bacterial
    Mutation
    Gene insert is codon optimized for Ecoli.
    Promoter
    T7/LacO
    Available Since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    156483-plasmid-map-sequence-id-307745

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  15. pB80-KIF1A(1-365)-VVDfast-GFP-SSPB(micro)

    Plasmid
    #174631
    Purpose
    Opto-kinesin for optogenetic heterodimerization to iLID via SSPB(micro)
    Depositor
    Lukas Kapitein
    Article
    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133
    Insert
    KIF1A(1-365)-VVDfast-GFP-SSPB(micro) (Kif1a Synthetic, Mouse)
    Tags
    GFP-SSPB(micro)
    Expression
    Mammalian
    Mutation
    mmKIF1A(aa1-365): Pro202Ala; ncVVD(aa37-184): Ile…
    Promoter
    Chicken beta-actin
    Available Since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    174631-plasmid-map-sequence-id-346982

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  17. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-B103 (LM2963)

    Plasmid
    #208960
    Purpose
    A variant CE1 construct with B103 DNA polymerase, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-B103-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A)
    Promoter
    CMV and T7
    Available Since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208960-plasmid-map-sequence-id-415786

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  20. pKK-mEGFP-TEV

    Plasmid
    #105777
    Purpose
    Expression of your protein of interest in fusion with green fluorescent protein at the N-terminus (cleavable by TEV). mEGFP is an EGFP A206K mutant with reduced dimerization (PMID: 11988576,22869113).
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    mEGFP-TEV
    Expression
    Mammalian
    Available Since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105777-plasmid-map-sequence-id-203366

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  21. pKK-TEV-mCherry

    Plasmid
    #105796
    Purpose
    Expression of your protein of interest in fusion with red fluorescent protein at the C-terminus (cleavable by TEV), (PMID: 15558047). mCherry descends from the first truly monomeric red FP mRFP1.
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    TEV-mCherry
    Expression
    Mammalian
    Available Since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105796-plasmid-map-sequence-id-203387

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  24. pETM33_Nsp7

    Plasmid
    #156476
    Purpose
    Bacterial expression of Sars-CoV2 Nsp7 protein with His-tag and GST-tag
    Depositor
    Ylva Ivarsson
    Article
    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:
    Insert
    Nsp7 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
    Tags
    His-GST
    Expression
    Bacterial
    Mutation
    Gene insert is codon optimized for Ecoli.
    Promoter
    T7/LacO
    Available Since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    156476-plasmid-map-sequence-id-307742

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  28. p-mCherry-N1-iChloC_CA

    Plasmid
    #98171
    Purpose
    slow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with blue to green light, inactivation max with 605 nm (accelarates closure to ms). Codon optimized for mammalian expression.
    Depositor
    Peter Hegemann
    Article
    Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.
    Insert
    Synthetic construct iChloC_C128A gene
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    E83Q, E90R, E101S, C128A, T159C, D156N
    Promoter
    CMV (+enhancer)
    Available Since
    July 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    98171-plasmid-map-sequence-id-182725

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  29. pETM33_Nsp8

    Plasmid
    #156477
    Purpose
    Bacterial expression of Sars-CoV2 Nsp8 protein with His-tag and GST-tag
    Depositor
    Ylva Ivarsson
    Article
    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:
    Insert
    Nsp8 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
    Tags
    His-GST
    Expression
    Bacterial
    Mutation
    Gene insert is codon optimized for Ecoli.
    Promoter
    T7/LacO
    Available Since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    156477-plasmid-map-sequence-id-307732

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  30. PF3D7_1136200-bio

    Plasmid
    #47730
    Purpose
    Expresses enzymatically monobiotinylated full-length PF3D7_1136200 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised PF3D7_1136200
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin replaced…
    Promoter
    CMV
    Available Since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47730-plasmid-map-sequence-id-68870

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Showing: 7021 - 7050 of 156048 results