We narrowed to 6,346 results for: Rel

1. 3xFLAG-DGKδ2

   Plasmid

   #226506

   Purpose

   Express N-terminal three tandem FLAG epitope tags, followed by an enterokinase cleavage site and human DGKD gene

   Depositor

   Fumio Sakane, Chiaki Murakami

   Article

   Murakami et al J Biol Chem. 2020 Mar 6;295(10):2932-294

   Insert

   DGKD (DGKD Human)
   

   Use

   Tags

   Three tandem FLAG epitope tag and enterokinase cl…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. Rab11 single guide RNA

   Plasmid

   #229683

   Purpose

   guide RNA for Rab11 tagging at the N-terminus in pX459

   Depositor

   Francesca Bottanelli

   Article

   Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

   Insert

   Rab11 guide (RAB11A Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pmch-mTet1-ZF-CD pc3956

   Plasmid

   #201709

   Purpose

   Expresses of mcherry-tagged mTet1-ZF-CD in mammalian cells

   Depositor

   Cristina Cardoso

   Article

   Arroyo et al Nat Commun. 2022 Sep 2;13(1):5173. doi:

   Insert

   Tet1-ZF_CD (Tet1 Mouse)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Zinc finger domain of Tet1 fused to its catalytic…

   Promoter

   CAG

   Available since

   July 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMRX-IP-muGFP-hATG2A mLIR

   Plasmid

   #215506

   Purpose

   Expresses GFP tagged human ATG2A with the mutation in LC3 interacting region.

   Depositor

   Noboru Mizushima

   Article

   Shimizu et al Hum Mol Genet. 2023 Aug 7;32(16):2623-26

   Insert

   Autophagy related 2A (ATG2A Human)
   

   Use

   Retroviral

   Tags

   muGFP

   Expression

   Mammalian

   Mutation

   P656R (natural variant with no functional relevan…

   Promoter

   Available since

   June 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCW57-Cx32D178Y-IRES-GCaMP6s

   Plasmid

   #216796

   Purpose

   Inducible bicistronic lentiviral plasmid for the simultaneous expression of the D178Y mutant form of Connexin 32 and cytosolic GCaMP6s

   Depositor

   Fabio Mammano

   Article

   Tettey-Matey et al Cell Commun Signal. 2024 Dec 5;22(1):589

   Insert

   Cx32D178Y-IRES-GCaMP6s (GJB1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   D178Y mutant form of Connexin 32

   Promoter

   Available since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-CMV-FLEX-SaCas9-U6-sgFAAH

   Plasmid

   #209197

   Purpose

   Mutagenesis of Faah

   Depositor

   Larry Zweifel

   Article

   Balsevich et al Elife. 2023 Apr 11;12:e81919. doi: 10.75

   Insert

   Faah (Faah Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Jan. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pT2/shCol1a1/Scarlet_Seq1.3

   Plasmid

   #206137

   Purpose

   Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

   Depositor

   Maria Castro

   Article

   Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

   Insert

   COL1A1 (Col1a1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pT2/shCol1a1/GFP4_Seq1.3

   Plasmid

   #206136

   Purpose

   Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

   Depositor

   Maria Castro

   Article

   Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

   Insert

   COL1A1 (Col1a1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pb-miRE-tre-3xFLAG-hnRNPU∆RGG

   Plasmid

   #196088

   Purpose

   Expresses mouse hnRNPU lacking RGG domain with a 3xFLAG tag in mammalian cells

   Depositor

   Mauro Calabrese

   Article

   Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

   Insert

   SAF-A (Hnrnpu Mouse)
   

   Use

   Tags

   N-terminal 3xFLAG

   Expression

   Bacterial and Mammalian

   Mutation

   removed last 154 amino acids on cterminal end

   Promoter

   TRE

   Available since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. CAGGS-safb∆dd1-NLS-3XFLAG-V5

    Plasmid

    #196092

    Purpose

    Expresses mouse SAFB lacking the first computationally called disordered domain in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tag

    Depositor

    Mauro Calabrese

    Article

    Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

    Insert

    SAFB (Safb Mouse)
    

    Use

    Tags

    C-terminal 3xFLAG and C-terminal V5

    Expression

    Bacterial and Mammalian

    Mutation

    Removed amino acids 96-285 (predicted disordered …

    Promoter

    CAGGS

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. CAGGS-safb∆dd3-NLS-3XFLAG-V5

    Plasmid

    #196094

    Purpose

    Expresses mouse SAFB lacking the third computationally called disordered domain in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tag

    Depositor

    Mauro Calabrese

    Article

    Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

    Insert

    SAFB (Safb Mouse)
    

    Use

    Tags

    C-terminal 3xFLAG and C-terminal V5

    Expression

    Bacterial and Mammalian

    Mutation

    Removed amino acids 638-925 (predicted disordered…

    Promoter

    CAGGS

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. CAGGS-SAFB-DD3-NLS-3XFLAG-V5

    Plasmid

    #196095

    Purpose

    Expresses only the third computationally called disordered domain of mouse SAFB in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tag

    Depositor

    Mauro Calabrese

    Article

    Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

    Insert

    SAFB (Safb Mouse)
    

    Use

    Tags

    C-terminal 3xFLAG and C-terminal V5

    Expression

    Bacterial and Mammalian

    Mutation

    Contains only AA 619-925 (NLS and predicted disor…

    Promoter

    CAGGS

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. eIF3k sgRNA-2 PX459 plasmid

    Plasmid

    #192256

    Purpose

    expressing sgRNA-2 targeting eIF3k locus closed to stop coden

    Depositor

    Dieter Wolf

    Article

    Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

    Insert

    sgRNA-2 targeting eIF3k locus closed to stop coden (EIF3K Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. eIF3k sgRNA-1 PX459 plasmid

    Plasmid

    #192255

    Purpose

    expressing sgRNA-1 targeting eIF3k locus closed to stop coden

    Depositor

    Dieter Wolf

    Article

    Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

    Insert

    sgRNA-1 targeting eIF3k locus closed to stop coden (EIF3K Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. eIF3f sgRNA-1 PX459 plasmid

    Plasmid

    #192253

    Purpose

    expressing sgRNA-1 targeting eIF3f locus closed to stop coden

    Depositor

    Dieter Wolf

    Article

    Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

    Insert

    sgRNA-1 targeting eIF3f locus closed to stop coden (EIF3F Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. eIF3f sgRNA-2 PX459 plasmid

    Plasmid

    #192254

    Purpose

    expressing sgRNA-2 targeting eIF3f locus closed to stop coden

    Depositor

    Dieter Wolf

    Article

    Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

    Insert

    sgRNA-2 targeting eIF3f locus closed to stop coden (EIF3F Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. eIF3b sgRNA-2 PX459 plasmid

    Plasmid

    #192250

    Purpose

    expressing sgRNA-2 targeting eIF3b locus closed to stop coden

    Depositor

    Dieter Wolf

    Article

    Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

    Insert

    sgRNA-2 targeting eIF3b locus closed to stop coden (EIF3B Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. eIF3b sgRNA-1 PX459 plasmid

    Plasmid

    #192249

    Purpose

    expressing sgRNA-1 targeting eIF3b locus closed to stop coden

    Depositor

    Dieter Wolf

    Article

    Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

    Insert

    sgRNA-1 targeting eIF3b locus closed to stop coden (EIF3B Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCG_SARS-CoV1 C-V5-tag ORF7a

    Plasmid

    #179971

    Purpose

    Mammalian expression vector for SARS-CoV-1 ORF7a, V5-tagged. Sequence codon-optimized.

    Depositor

    Konstantin Sparrer

    Article

    Hayn et al Cell Rep. 2021 May 18;35(7):109126.

    Insert

    SARS-CoV-1 ORF7a (ORF7a Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCG_SARS-CoV1 NSP7 C-V5-tag

    Plasmid

    #179963

    Purpose

    Mammalian expression vector for SARS-CoV-1 Nsp7, V5-tagged. Sequence codon-optimized.

    Depositor

    Konstantin Sparrer

    Article

    Hayn et al Cell Rep. 2021 May 18;35(7):109126.

    Insert

    SARS-CoV-1 Nsp7 (ORF1ab Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    human codon-optimized

    Promoter

    Available since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge