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Plasmid#198049PurposeMoClo Golden Gate Level 1 Position 6. Overexpression cassette of Growth-Regulating Factor 4 (GRF4) plus GRF-Interacting Factor 1 (GRF4-GIF1). Improves in vitro wheat regeneration and transformation.InsertZmUbiP::TaGRF4-GIF1::NosT
UseSynthetic BiologyPromoterZea mays (maize) ubiquitin promoterAvailable SinceApril 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGADT7-AP-1 σ1B
Plasmid#198335PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-1 σ1B fusion protein in yeast (yeast two-hybrid assays)DepositorInsertAP-1 σ1B
TagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastMutationExpresses a LELQMNRRY nonapeptide after the last …PromoterADH1Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGADT7-AP-1 σ1A
Plasmid#198334PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-1 σ1A fusion protein in yeast (yeast two-hybrid assays)DepositorInsertAP-1 σ1A
TagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastMutationExpresses a LELQMNRRY nonapeptide after the last …PromoterADH1Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO/TARG1
Plasmid#172580PurposeFor generation of FlpIn lines with untagged TARG1DepositorAvailable SinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
Mito-CoilE-mEm
Plasmid#181994PurposeCoilE genetically encoded tag on Mito-mEmerald, to be used with CoilR labeled with a reporterDepositorInsertMito-CoilE-mEm
TagsmEmeraldExpressionMammalianAvailable SinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
Antares2-N1
Plasmid#120869PurposePiggybac transposon plasmid for live animal optical imagingDepositorInsertAkaluc
ExpressionMammalianPromoterCMV PromoterAvailable SinceFeb. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-TK-Firefly-cjCD69
Plasmid#122279PurposeLentiviral vector for stable expression of Firefly luciferase.DepositorInsertsFirefly luciferase
cjCD69 3'UTR
UseLentiviral and LuciferaseExpressionMammalianAvailable SinceApril 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-TK-Firefly-cjMGA
Plasmid#122280PurposeLentiviral vector for stable expression of Firefly luciferase.DepositorInsertsFirefly luciferase
cjMGA 3'UTR
UseLentiviral and LuciferaseExpressionMammalianAvailable SinceApril 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-TK-Firefly-cjPACS1
Plasmid#122281PurposeLentiviral vector for stable expression of Firefly luciferase.DepositorInsertsFirefly luciferase
cjPACS1 3'UTR
UseLentiviral and LuciferaseExpressionMammalianAvailable SinceApril 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-TK-Firefly-cjSTK4
Plasmid#122282PurposeLentiviral vector for stable expression of Firefly luciferase.DepositorInsertsFirefly luciferase
cjSTK4 3'UTR
UseLentiviral and LuciferaseExpressionMammalianAvailable SinceApril 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-TK-Firefly-cjTP53RK
Plasmid#122283PurposeLentiviral vector for stable expression of Firefly luciferase.DepositorInsertsFirefly luciferase
cjTP53RK 3'UTR
UseLentiviral and LuciferaseExpressionMammalianAvailable SinceApril 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-TK-Firefly-cjYTHDC1
Plasmid#122284PurposeLentiviral vector for stable expression of Firefly luciferase.DepositorInsertsFirefly luciferase
cjYTHDC1 3'UTR
UseLentiviral and LuciferaseExpressionMammalianAvailable SinceApril 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
P302-FBP_1
Plasmid#139692PurposeT-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway, without recycling gene CPHDepositorInsertp35S_Short_TMV 5':AsNpga:tAtug7, AtUbi10:NnH3H:AtuOCS, pCmYLCV:NnHisps:tHsp, pAtuMas:NnLuz:trbcSE9
UseLuciferaseExpressionPlantPromotervariousAvailable SinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
P313-FBP_8
Plasmid#139699PurposeT-DNA encoding Fungal Bioluminescent Pathway Components, control pathway, missing H3HDepositorInsertpLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pCmYLCV:NnH3H:AtuOcs, pCmYLCV:NnHisps:tHsp, pAtuMas:Nn Luz:trbcSE9
UseLuciferaseExpressionPlantPromotervariousAvailable SinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046858
Plasmid#145658PurposeBacterial Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferaseDepositorInsertSARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;HAExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046775
Plasmid#145636PurposeBacterial Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferaseDepositorInsertSARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;MycExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046827
Plasmid#145625PurposeBacterial Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferaseDepositorInsertSARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;HAExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046823
Plasmid#145634PurposeBacterial Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferaseDepositorInsertSARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;MycExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046816
Plasmid#145622PurposeBacterial Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferaseDepositorInsertSARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;FLAG_2ExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046824
Plasmid#145613PurposeBacterial Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferaseDepositorInsertSARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;FLAG_2ExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046791
Plasmid#145586PurposeBacterial Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferaseDepositorInsertSARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;StrepIIExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
psiCHECK3-TMED3-Reverse-Complement
Plasmid#136018PurposeThe Reverse Complement of TMED3 3'UTR inserted into the 3'UTR of Renilla luciferase in the psiCHECK3 plasmidDepositorAvailable SinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK3-ZC3H15-Reverse-Complement
Plasmid#136019PurposeThe Reverse Complement of ZC3H15 3'UTR inserted into the 3'UTR of Renilla luciferase in the psiCHECK3 plasmidDepositorAvailable SinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
MtMscL delta49
Plasmid#118342PurposeM. tuberculosis MscL (delta49) in pET19bDepositorInsertMscL (mscL Mycobacterium tuberculosis)
Tags6x His Tag and Thrombin Cleavage siteExpressionBacterialMutationChanged Glutamic Acid (Glu) 102 to Stop CodonPromoterT7 PromoterAvailable SinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)-LUCIDRS-MTX
Plasmid#113925PurposePlasmid encoding the bacterial expression of the red-shifted LUCID semi-synthetic BRET biosensor for methotrexateDepositorInsertLUCID_RS-MTX
UseLuciferaseExpressionBacterialPromoterT7Available SinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFL-SV40-PACT 5'-UTR
Plasmid#115351Purposefirefly luciferase (Fluc) reporter containing the 5’-UTR of PACTDepositorAvailable SinceSept. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJOG659
Plasmid#105410PurposeSynthetic biologyDepositorInsertLuciferase CDS fused to 3xHA tag
UseSynthetic BiologyMutationBsaI/ BpiI restriction sites removedAvailable SinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJFRC81-3xVAS4-IVS-Syn21-GFP-P10
Plasmid#104615PurposeExpresses GFP upon binding of TALE4 to VAS4DepositorInsert3xVAS4
ExpressionInsectPromoterhsp70Available SinceJan. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGL3_zfh1_CP-candidate_luc+
Plasmid#86391PurposeLuciferase validation vector, where candidates are inserted at the position of the core promoter. The candidates responsiveness to the upstream enhancer is reflected by luciferase activityDepositorInsertD. melanogaster zfh1 enhancer
UseLuciferaseAvailable SinceMarch 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Scmh1-3'UTR
Plasmid#61785PurposeLuciferase reporter containing the 3'UTR of mouse Scmh1DepositorInsertmouse Scmh1 3'UTR
UseLuciferaseAvailable SinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only