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1025

Plasmid

#160235

Purpose

FlightN-TetR(OFF)-VP16-Sv40-attB

Depositor

Omar Akbari

Article

Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.

Inserts

Tetracycline repressor
Flightin promoter

Tags

VP16

Expression

Insect

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pCellFree_G06

Plasmid

#67140

Purpose

Gateway destination vector for cell-free expression with C-term mCherry 8xHis tag

Depositor

Kirill Alexandrov

Article

Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

Type

Empty backbone

Use

Cell-free expression

Tags

HIS and mCherry

Available Since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pJet1.2 Tev-Halo tag

Plasmid

#117124

Purpose

C-terminal Tev Halo-Tag

Depositor

Stephan Gruber

Article

Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

Insert

Tev-Halo-tag Tag

Use

Golden gate donor vector for gene targeting in ba…

Tags

Tev-Halo-Tag

Available Since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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P0050::rpaA (KmR)

Plasmid

#102321

Purpose

drives expression of rpaA from an rpaA-independent promoter (kanamycin resistance casette)

Depositor

Erin O'Shea

Article

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

RpaA with p0050 promoter (+ kanamycin resistance casette)

Use

Cloning vector

Promoter

-

Available Since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pET29b_Qcat9

Plasmid

#45137

Depositor

Christopher Petzold

Article

Batth et al Metab Eng. 2014 Sep 6. pii: S1096-7176(1

Insert

E-coli-pathways-QconCAT9

Tags

6XHis

Expression

Bacterial

Promoter

T7

Available Since

Aug. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pET29b_Qcat10

Plasmid

#45138

Depositor

Christopher Petzold

Article

Batth et al Metab Eng. 2014 Sep 6. pii: S1096-7176(1

Insert

E-coli-pathways-QconCAT10

Tags

6XHis

Expression

Bacterial

Promoter

T7

Available Since

Aug. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pET29b_Qcat3

Plasmid

#45131

Depositor

Christopher Petzold

Article

Batth et al Metab Eng. 2014 Sep 6. pii: S1096-7176(1

Insert

E-coli-pathways-QconCAT3

Tags

5XHis

Expression

Bacterial

Promoter

T7

Available Since

Aug. 7, 2014

Availability

Academic Institutions and Nonprofits only

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mRuby2-NT-C1

Plasmid

#54561

Purpose

Localization: C1 Cloning Vector, Excitation: 559, Emission: 600

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Type

Empty backbone

Tags

mRuby2-NT

Expression

Mammalian

Available Since

June 22, 2015

Availability

Industry, Academic Institutions, and Nonprofits
AP568-3

Plasmid

#70048

Purpose

co-expression of Cas9 and crRNA 589 target sequence

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2015 Sep;201(1):47-54. doi: 10

Insert

sgRNA for dpy‐10

Use

CRISPR

Expression

Worm

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-Flag-YAP-R89A

Plasmid

#33058

Depositor

Kunliang Guan

Article

Li et al Genes Dev. 2010 Feb 1;24(3):235-40.

Insert

YAP (YAP1 Human)

Tags

Flag

Expression

Mammalian

Mutation

R89A

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
pMK388 (mClover-BSD)

Plasmid

#121189

Purpose

C-terminal tagging

Depositor

Masato Kanemaki

Article

Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

Insert

mClover-BSD

Use

CRISPR

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pCellFree_G09

Plasmid

#67143

Purpose

Gateway destination vector for cell-free expression with N-term 8xHis sfGFP tag

Depositor

Kirill Alexandrov

Article

Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

Type

Empty backbone

Use

Cell-free expression

Tags

His and sfGFP

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-Flag-YAP-L91A

Plasmid

#33064

Depositor

Kunliang Guan

Article

Li et al Genes Dev. 2010 Feb 1;24(3):235-40.

Insert

YAP (YAP1 Human)

Tags

Flag

Expression

Mammalian

Mutation

L91A

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
TAL2368

Plasmid

#36779

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

NCOR1-TALEN_Left (Ncor1 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
p202_LTJ_sgRNACD45.2_R1

Plasmid

#82672

Purpose

sgRNA targeting murine CD45.2 region 1. Co-expresses SpCas9-2A-EGFP.

Depositor

Lukas Jeker

Article

Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

Insert

sgRNA targeting mouse CD45.2 region 1

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCRII-SNORD115-11

Plasmid

#45890

Depositor

Stefan Stamm

Article

Falaleeva et al Clin Med Insights Case Rep. 2013 May 5;6

Insert

SNORD115 without flanking exons and intron (SNORD115-11 Human)

Use

Unspecified

Available Since

Aug. 5, 2013

Availability

Academic Institutions and Nonprofits only
pET-TALE-mCherry-15b

Plasmid

#138287

Purpose

Express tTALE-mCherry

Depositor

Kyubong Jo

Article

Shin et al Sci Rep. 2019 Nov 20;9(1):17197. doi: 10

Insert

7-mer tTALE-mCherry

Use

TALEN

Tags

6x His-tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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pHPdestVenus

Plasmid

#24568

Depositor

Ingrid Lohmann

Article

Boy et al Genesis. 2010 Apr 22. [Epub ahead of pri

Insert

Use

Reporter construct

Tags

Venus

Available Since

May 19, 2010

Availability

Academic Institutions and Nonprofits only

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pYPQ141-ZmUbi-RZ-Aa3.8.5

Plasmid

#158407

Purpose

Express single gRNA with Aa3.8.5 scaffold (with four MS2 binding sites) under ZmUbi promoter

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

Maize ubiquitin 1

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pTRE/WT SP with 5'UTR

Plasmid

#89330

Purpose

Inducible expression of WT spastin M1 and M87 in mammalian cells

Depositor

Peter Baas

Article

Solowska et al J Neurosci. 2014 Jan 29;34(5):1856-67. d

Insert

spastin (SPAST Human)

Expression

Mammalian

Promoter

Tet-responsive P tight

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pVD_85

Plasmid

#149604

Purpose

(parent plasmid pCFB257) pX - 3 -LoxP -KlLEU2 -pTEF1 - XylR

Depositor

Michael Jensen

Article

Ambri et al ACS Synth Biol. 2020 Feb 21;9(2):218-226

Insert

XylR

Use

Synthetic Biology

Tags

SV40

Expression

Yeast

Promoter

TEF1

Available Since

Dec. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pFA-MNase-CaURA3

Plasmid

#162461

Purpose

DNA of the 3xFLAG epitope-MNase module for C. albicans. The PacI-AscI 3xFLAG-MNase fragment was cloned in the PacI-AscI-digested pFA-TAP-CaURA3

Depositor

Adnane Sellam

Article

Tebbji et al mSphere. 2020 Oct 14;5(5). pii: 5/5/e006

Insert

3FLAG-Mnase

Expression

Yeast

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pFA-MNase-CaHIS1

Plasmid

#162462

Purpose

DNA of the 3xFLAG epitope-MNase module for C. albicans. The PacI-AscI 3xFLAG-MNase fragment was cloned in the PacI-AscI-digested pFA-TAP-Ca HIS1

Depositor

Adnane Sellam

Article

Tebbji et al mSphere. 2020 Oct 14;5(5). pii: 5/5/e006

Insert

3FLAG-Mnase

Expression

Yeast

Available Since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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MXS_Tet

Plasmid

#62422

Purpose

MXS_chaining vector with Tet

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

Tetracycline inducible promoter

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pTC208

Plasmid

#70017

Purpose

TALENs targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based onTomato Yellow Leaf Curl Virus

Depositor

Daniel Voytas

Article

Cermak et al Genome Biol. 2015 Nov 6;16(1):232. doi:

Insert

Nuclease (TALENs) + Donor + GVR

Use

TALEN

Expression

Plant

Available Since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pET29b_Qcat5

Plasmid

#45133

Depositor

Christopher Petzold

Article

Batth et al Metab Eng. 2014 Sep 6. pii: S1096-7176(1

Insert

E-coli-pathways-QconCAT5

Tags

6XHis

Expression

Bacterial

Promoter

T7

Available Since

Jan. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pET29b_Qcat2

Plasmid

#45130

Depositor

Christopher Petzold

Article

Batth et al Metab Eng. 2014 Sep 6. pii: S1096-7176(1

Insert

E-coli-pathways-QconCAT2

Tags

6XHis

Expression

Bacterial

Promoter

T7

Available Since

Dec. 12, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pET29b_Qcat6

Plasmid

#45134

Depositor

Christopher Petzold

Article

Batth et al Metab Eng. 2014 Sep 6. pii: S1096-7176(1

Insert

E-coli-pathways-QconCAT6

Tags

6XHis

Expression

Bacterial

Promoter

T7

Available Since

Aug. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pMLS719

Plasmid

#188894

Purpose

miniMos Arm sgRNA expression vector

Depositor

Erik Jorgensen

Article

Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

Insert

PU6(K09B11.12)::miniMos Arm sgRNA

Expression

Worm

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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TAL2218

Plasmid

#36677

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

MUTYH-TALEN_Left (Mutyh Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only