We narrowed to 27,285 results for: Tat
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pcDNA3-FLAG-HA-CAD S1900A
Plasmid#46240PurposeFLAG-HA-CAD with S1900 mutated to alanineDepositorInsertcarbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (Cad Mouse)
UseTagsFLAG and HAExpressionMammalianMutationSerine 1900 changed to Alanine (S1900A)PromoterCMVAvailable sinceNov. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
3021_pETcon-SARS-CoV-2-RBD_K417N_E484K_N501Y
Plasmid#184406Purposeyeast surface display of the SARS-CoV-2 Beta variant RBDDepositorInsertSARS-CoV-2 Beta Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)
UseTagsHA and c-MycExpressionYeastMutationK417N+E484K+N501YPromoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti PA-mCit-NL
Plasmid#113452PurposeLentiviral ProteinA-mCitrine-NanoLuc hybrid-fusion control expression vector (positive control)DepositorInsertProteinA-mCitrine-NanoLuc
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterhUBbCAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCR8/GW/TOPO-ELK1
Plasmid#98616PurposeGateway entry TOPO TA cloning vector for ELK1DepositorInsertELK1 (Elk1 Mouse)
UseTopo ta cloning vectorTagsExpressionMutationQ230RPromoterAvailable sinceSept. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-AR-S81E
Plasmid#171224PurposeMammalian expression of un-tagged human androgen receptor phosphorylation mutant: AR-Ser81Glu (AR-S81E)DepositorInserthuman androgen receptor Ser81Glu mutant (AR Human)
UseTagsExpressionBacterial and MammalianMutationAR-Ser81Glu (AR-S81E)PromoterCMVAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
RCAS-sgATG7
Plasmid#75228PurposeAn adaption on the RCAS/tv-a somatic cell gene transfer system, for use in combination with an existing Cas9 background in the cell/mouse of interest. Depositors: Jane Fraser/Noor GammohDepositorInsertAutophagy Related 7 (Atg7 Mouse)
UseRetroviralTagsExpressionMutationPromoterU6Available sinceJune 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-RIα(D/D+linker)-EGFP
Plasmid#181842PurposeEGFP-tagged docking/dimerization (D/D) and linker region of PKA RIα; for mammalian expression.DepositorInsertRIα-(D/D+linker)EGFP (PRKAR1A Human)
UseTagsEGFPExpressionMammalianMutationContains only residues 12-112 of PKA RIα.PromoterCMVAvailable sinceJune 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-hDDB2-T338M-V5
Plasmid#167470Purposeectopic expression of hDDB2 in mammalian cellsDepositorInsertDDB2 (DDB2 Human)
UseTags6xHis and V5ExpressionMammalianMutationT338MPromoterCMVAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMY-Ly6G-Rluc_miR
Plasmid#163339PurposeRetroviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase and expression of a Ly6G surface markerDepositorInsertLy-6G (Ly6g Mouse)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterLTRAvailable sinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-hDDB2-K244E-V5
Plasmid#167471Purposeectopic expression of hDDB2 in mammalian cellsDepositorInsertDDB2 (DDB2 Human)
UseTags6xHis and V5ExpressionMammalianMutationK244EPromoterCMVAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pXPR_BRD003-sgSNAI1-2097
Plasmid#115453PurposeConstitutive lentiviral expressionDepositorInsertsgSNAI1-2097 (SNAI1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
plko-tet-on-puromycin-shTRX1-259
Plasmid#110920PurposeTo knockdown Thioredoxin-1 following doxycycline additionDepositorInsertTRX1 (TXN Human)
UseLentiviralTagsExpressionMutationPromoterunknownAvailable sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag4A-MMP8 (P78S)
Plasmid#29546DepositorInsertMetalloproteinase protein 8 (MMP8 Human)
UseTagsFLAGExpressionBacterial and MammalianMutationP78SPromoterAvailable sinceJuly 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLV-PLXNB2-decto
Plasmid#86238PurposeLentivirus for expression of human Plexin-B2 with deletion of ectodomainDepositorInsertPlexin-B2 (PLXNB2 Human)
UseLentiviralTagsExpressionMammalianMutationPLXNB2 with deletion of extracellular domainPromoterhPGKAvailable sinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-PLXNB2-dVTDL
Plasmid#86239PurposeLentivirus for expression of human Plexin-B2 with deletion of C-terminal VTDL aminoacidsDepositorInsertPlexin-B2 (PLXNB2 Human)
UseLentiviralTagsExpressionMammalianMutationPLXNB2 with deletion of last 4 aminoacidsPromoterhPGKAvailable sinceJune 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUDR217
Plasmid#113872Purposeexpression of Cas9 programming sgRNA9 and sgRNA10 targetting MPH2-3 and MAL11 respectivelyDepositorInsertsgRNA9-MPH2-3 sgRNA10-MAL11
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceSept. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEN_hU6miR-G12-E
Plasmid#25757PurposeEntry vector with human U6 promoter driving mouse G alpha 12 miR30-based shRNA.DepositorInsertG alpha 12 miR-shRNA (Gna12 Mouse)
UseEntry vectorTagsExpressionMutationPromoterAvailable sinceJuly 14, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-hDIXDC1 S592A
Plasmid#61223PurposeMammalian expression vector encoding long isoform of human DIXDC1DepositorInserthuman DIXDC1 (DIXDC1 Human)
UseTagsFLAGExpressionMammalianMutationChanged Serine 592 to AlaninePromoterCMVAvailable sinceJan. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
Myc-Idax DBM
Plasmid#68554PurposeThis plasmid expresses full length IDAX containing a DNA binding mutation.DepositorInsertIDAX-DBM (Cxxc4 Mouse)
UseTagsMycExpressionMammalianMutationT162A, H164A and Q165A mutationsPromoterEF1aAvailable sinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO/SH/GW-Mst2
Plasmid#84300PurposeExpresses a tetracycline-inducible SH-tagged (streptavidin-binding peptide and HA tag) version of Mst2. Used in conjunction with Flp-In cells to generate single copy transgene.DepositorInsertMst2 (STK3 Human)
UseTagsHA, SBPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDEST-EGFP-Cx43ML-N1
Plasmid#49860PurposeEncodes human connexin 43 with all internal methionines mutated to leucine and a C_terminal EGFP fusion tagDepositorInsertConnexin 43 (GJA1 Human)
UseTagsEGFPExpressionMammalianMutationAll internal Methionines mutated to Leucine. No s…PromoterCMVAvailable sinceDec. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEF5/FRT/DEST-3xHA/mGli2 P5-6D
Plasmid#51254PurposeEncodes N-3xHA-TEV-mouseGli2-C; amino acids 923,956 mutated to AspDepositorInsertGli2 (Gli2 Mouse)
UseFlpin systemTags3xHA-TEVExpressionMammalianMutationamino acids 923,956 mutated to AspPromoterAvailable sinceFeb. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
FUW TetO NET1A-V5 WT
Plasmid#69828PurposeExpresses NET1A-V5 in mammalian cells in a doxycycline inducible mannerDepositorInsertNeuroepithelial cell-transforming gene 1 protein A (NET1 Human)
UseLentiviralTagsv5ExpressionMammalianMutationPromoterminimal CMV promoter with tetracycline operatorAvailable sinceApril 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
PHAGE-P CMVt N-HA hE6-AP I (AA 1-500)
Plasmid#119312Purposelentiviral vector for expression of HA-Tagged E6-AP I AA 1-500 in mammalian cellsDepositorInsertE6-AP I (UBE3A Human)
UseLentiviralTagsHAExpressionMutationOnly AA 1-500PromoterCMVAvailable sinceJuly 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
PHAGE-P CMVt N-HA hE6-AP I (AA 1-376)
Plasmid#119311Purposelentiviral vector for expression of HA-Tagged E6-AP I AA 1-376 in mammalian cellsDepositorInsertE6-AP I (UBE3A Human)
UseLentiviralTagsHAExpressionMutationOnly AA 1-376PromoterCMVAvailable sinceJuly 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
PHAGE-P CMVt N-HA hE6-AP I (AA 481-852)
Plasmid#119316Purposelentiviral vector for expression of HA-Tagged E6-AP I AA 481-852 in mammalian cellsDepositorInsertE6-AP I (UBE3A Human)
UseLentiviralTagsHAExpressionMutationOnly AA 481-852PromoterCMVAvailable sinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-CCR5(R5gp120-Hi)
Plasmid#98954PurposeMammalian expression plasmid for myc-tagged human CCR5 mutant R5gp120-HiDepositorInsertCCR5 (CCR5 Human)
UseTagsSignal/leader sequence from Influenza A virus HA …ExpressionMammalianMutationR5gp120-Hi variant: P8L, I9E, I12M, S17E, P19D, Q…PromoterCMVAvailable sinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
TRPM7 gRNA (BRDN0001146152)
Plasmid#76111Purpose3rd generation lentiviral gRNA plasmid targeting human TRPM7DepositorInsertTRPM7 (TRPM7 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pXPR_BRD003-sgRBFOX1-2083
Plasmid#115451PurposeConstitutive lentiviral expressionDepositorInsertsgRBFOX1-2083 (RBFOX1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
B-eSpCas9
Plasmid#126761PurposeExpression plasmid for human codon-optimized increased fidelity Blackjack-eSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than eSpCas9.DepositorInsertB-eSpCas9
UseCRISPRTags3xFLAG and NLSExpressionMammalianMutationK848A; K1003A; R1060A; amino acids 1005-1013 repl…PromoterCBhAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV_3xgRNA;PTENA, p53B,SMAD4A_CAG_FlPO_synthPA
Plasmid#68346PurposeUsed to produce AAV expressing the FlpO recombinase and pig gRNA towards 3 tumor suppressors: PTEN, p53 and SMAD4DepositorInserts3xgRNA;PTENA, p53B,SMAD4A
FlPO
UseAAV; Flp/frtTagsExpressionMammalianMutationPromoterCAG and U6Available sinceDec. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
B-evoSpCas9
Plasmid#126765PurposeExpression plasmid for human codon-opt. increased fidelity Blackjack-evoSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than evoSpCas9.DepositorInsertB-evoSpCas9
UseCRISPRTags3xFLAG and NLSExpressionMammalianMutationM495V; Y515N; K526E; R661Q; amino acids 1005-1013…PromoterCBhAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSN67
Plasmid#100124Purposeexpresses putative KLF4 DBD with a 6xHis tagDepositorInsertmCherry-P2A-putative KLF4 DBD with a 6xHis tag (KLF4 Human)
UseLentiviralTagsExpressionMammalianMutationInsert corresponds to the DBD of KLF4PromoterAvailable sinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1Xpress-BcrGAP R1090A
Plasmid#38176DepositorInsertBCR GAP domain encoding residues 1004-1271 with R1090A point mutation (BCR Human)
UseTagsXpressExpressionMammalianMutationaa 1004-1271 with R1090A point mutationPromoterAvailable sinceSept. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
AcrIIC1X* (AcrIIC1X-mCherry chimera)
Plasmid#128114PurposeExpresses AcrIIC1(N3F/D15Q/A48I) fused to mCherry; mediates potent inhibition of both, N. meningitidis as well as S. aureus Cas9DepositorInsertAcrX* (AcrIIC1 N3F/D15Q/A48I fused to mCherry)
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pURD214
Plasmid#113871Purposeexpression of Cas9 programming sgRNA5 and sgRNA2 targetting HXT13-15-16 and HXT2 respectivelyDepositorInsertsgRNA5 HXT13-15-16 sgRNA2-HXT2
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceSept. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.2/V5-DEST NET1A S46A
Plasmid#69818PurposeExpresses NET1A-V5 in mammalian cellsDepositorInsertNeuroepithelial cell-transforming gene 1 protein A (NET1 Human)
UseTagsV5ExpressionMammalianMutationS46APromoterCMVAvailable sinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
hnRNPA2_LC_P298L_S285C
Plasmid#104469Purposeexpress His tagged P298L S285C hnRNPA2 LCDepositorInserthnRNPA2 (HNRNPA2B1 Human)
UseTagsHisExpressionBacterialMutationP298L S285CPromoterAvailable sinceJan. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
SLC30A10-delta105-107
Plasmid#65205PurposeConstitutive expression of SLC30A10 delta 105-107 in mammalian cellsDepositorInsertSLC30A10 (SLC30A10 Human)
UseTagsFlagExpressionMammalianMutationdelta 105-107 amino acidsPromoterCMVAvailable sinceMay 19, 2015AvailabilityAcademic Institutions and Nonprofits only
