We narrowed to 14,011 results for: flag

1. pLEX-FHH-MAPKAP1RBD

   Plasmid

   #120710

   Purpose

   Expresses Flag-HA-6xHIS-MAPKAP1 RBD fusion protein in mammalian cells & for virus production

   Depositor

   Paul Khavari

   Article

   Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   MAPKAP1 RBD
   

   Use

   Lentiviral

   Tags

   Flag-HA-6xHIS

   Expression

   Mammalian

   Mutation

   Amino acids 279-353

   Promoter

   CMV

   Available Since

   Jan. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNCMV HPV8 E6 del132-136

   Plasmid

   #87658

   Purpose

   Flag/HA tagged HPV8 E6 del132-136

   Depositor

   Karl Munger

   Article

   Meyers et al PLoS Pathog. 2017 Jan 20;13(1):e1006171.

   Insert

   HPV 8E6
   

   Use

   Tags

   Flag and HA

   Expression

   Mammalian

   Mutation

   Deletion of aa132-136

   Promoter

   CMV

   Available Since

   March 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRS314-4M5.3

   Plasmid

   #45830

   Depositor

   Dane Wittrup

   Insert

   4M5.3
   

   Use

   Tags

   FLAG

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available Since

   June 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRS316-4M5.3

   Plasmid

   #45831

   Depositor

   Dane Wittrup

   Insert

   4M5.3
   

   Use

   Tags

   FLAG

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available Since

   June 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. FNpCDNA3

   Plasmid

   #45346

   Depositor

   Robert Oshima

   Type

   Empty backbone

   Use

   Tags

   FLAG and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-U6-racRNA-A

   Plasmid

   #200824

   Purpose

   AAV transfer plasmid encoding a circularized RNA barcode A under U6 promoter and a subcellular localization protein binder under hSyn promoter

   Depositor

   Xiao Wang

   Article

   Shi et al Nature. 2023 Oct;622(7983):552-561. doi:

   Inserts

   U6-racRNA
   V5-PP7cp-M9-NES-T2A-Flag-PP7cp-Far
   

   Use

   AAV

   Tags

   C-Far, Flag, and V5

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTripz NEO DEST

   Plasmid

   #209072

   Purpose

   Modified DOX-ON inducible destination vector with a c-terminal 3X flag tag to be used for LR recombination reactions to inducibly express cDNAs of interest

   Depositor

   Matthew Oser

   Article

   Oser et al Cancer Discov. 2019 Feb;9(2):230-247. do

   Type

   Empty backbone

   Use

   Lentiviral; Gateway destination vector to be used…

   Tags

   3X FLAG

   Expression

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-U6-racRNA-B

   Plasmid

   #200825

   Purpose

   AAV transfer plasmid encoding a circularized RNA barcode B under U6 promoter and a subcellular localization protein binder under hSyn promoter

   Depositor

   Xiao Wang

   Article

   Shi et al Nature. 2023 Oct;622(7983):552-561. doi:

   Inserts

   U6-racRNA
   V5-PP7cp-M9-NES-T2A-Flag-PP7cp-Far
   

   Use

   AAV

   Tags

   C-Far, Flag, and V5

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-U6-racRNA-C

   Plasmid

   #200826

   Purpose

   AAV transfer plasmid encoding a circularized RNA barcode C under U6 promoter and a subcellular localization protein binder under hSyn promoter

   Depositor

   Xiao Wang

   Article

   Shi et al Nature. 2023 Oct;622(7983):552-561. doi:

   Inserts

   U6-racRNA
   V5-PP7cp-M9-NES-T2A-Flag-PP7cp-Far
   

   Use

   AAV

   Tags

   C-Far, Flag, and V5

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-U6-racRNA-D

    Plasmid

    #200827

    Purpose

    AAV transfer plasmid encoding a circularized RNA barcode D under U6 promoter and a subcellular localization protein binder under hSyn promoter

    Depositor

    Xiao Wang

    Article

    Shi et al Nature. 2023 Oct;622(7983):552-561. doi:

    Inserts

    U6-racRNA
    V5-PP7cp-M9-NES-T2A-Flag-PP7cp-Far
    

    Use

    AAV

    Tags

    C-Far, Flag, and V5

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pgLAP4

    Plasmid

    #19705

    Depositor

    Peter Jackson

    Insert

    pgLAP4
    

    Use

    Tags

    Flag, S peptide, and Tev

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFETCh_RAD21

    Plasmid

    #64056

    Purpose

    Homology Arms and 3X Flag with P2A Neo for 3' tagging of human RAD21

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    RAD21 Homology Arms (RAD21 Human)
    

    Use

    Tags

    3X Flag P2A Neo

    Expression

    Mutation

    Promoter

    Available Since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMH-SFB

    Plasmid

    #99391

    Purpose

    Gateway-compatible mammalian expression destination vector for creating SFB N-terminal fusions (S protein tag, Flag tag, Streptavidin binding peptide)

    Depositor

    Michael Huen

    Article

    An et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

    Type

    Empty backbone

    Use

    Tags

    SFB (S protein-FLAG-Streptavidin binding peptide)

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCW57.1-Tet-UPF1WT (pRKB264)

    Plasmid

    #99146

    Purpose

    Inducible lentiviral construct to express FLAG-tagged UPF1wt

    Depositor

    Robert Bradley

    Article

    Feng et al Mol Cell. 2017 Jul 20;67(2):239-251.e6.

    Insert

    UPF1 (UPF1 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    First 127 codons are optimized to reduce GC conte…

    Promoter

    pTight

    Available Since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLexM-SLC7A11_GFP

    Plasmid

    #197995

    Purpose

    Expresses SLC7A11 with an N-terminal FLAG tag and C-terminal TEV-GFP-His8 tag in mammalian cells

    Depositor

    Simon Newstead

    Article

    Parker et al Nat Commun. 2021 Dec 8;12(1):7147. doi:

    Insert

    SLC7A11 (SLC7A11 Human)
    

    Use

    Tags

    FLAG tag and TEV-GFP-His8

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDH-EF1-FHC

    Plasmid

    #64874

    Purpose

    lentiviral vector with Flag, HA tags

    Depositor

    Richard Wood

    Article

    Yousefzadeh et al PLoS Genet. 2014 Oct 2;10(10):e1004654.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTP288

    Plasmid

    #113460

    Purpose

    human Flag tagged Nbs1 in baculovirus transfer vector

    Depositor

    Tanya Paull

    Article

    Lee et al J Biol Chem. 2013 May 3;288(18):12840-51

    Insert

    Nbs1 (NBN Human)
    

    Use

    Tags

    Flag

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available Since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCALNL-NaChBac

    Plasmid

    #125580

    Purpose

    Cre-dependent expression of NaChBac (FLAG-tagged) in mammalian cells.

    Depositor

    Takeshi Imai

    Article

    Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

    Insert

    NaChBac
    

    Use

    Cre/Lox

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. HBT-pcoCas9

    Plasmid

    #52254

    Purpose

    transient expression of pcoCas9 gene in plant cells

    Depositor

    Jen Sheen

    Article

    Li et al Nat Biotechnol. 2013 Aug;31(8):688-91. d

    Insert

    pcoCas9
    

    Use

    CRISPR; Plant expression

    Tags

    FLAG and NLS

    Expression

    Mutation

    Promoter

    hybrid constitutive promoter 35SPPDK

    Available Since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMH-SFB-RNF8

    Plasmid

    #99396

    Purpose

    Mammalian expression construct for S protein-Flag-Streptavidin binding peptide (SFB)-tagged RNF8

    Depositor

    Michael Huen, Junjie Chen

    Article

    Huen et al Cell. 2007 Nov 30;131(5):901-14. Epub 20

    Insert

    RNF8 (RNF8 Human)
    

    Use

    Tags

    SFB (S protein-FLAG-Streptavidin binding peptide)

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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21. pCambia-PUP-IT

    Plasmid

    #186478

    Purpose

    Expresses POI-PUP-IT fusion in Plants

    Depositor

    Jen Sheen

    Article

    Ye et al Nature. 2022 Sep 14. pii: 10.1038/s41586

    Insert

    FLAG-Pup(E)
    

    Use

    Tags

    Triple Flag Tag

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available Since

    Sept. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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22. pcDNA4-ATR-C

    Plasmid

    #53769

    Purpose

    mammalian expression of flag-tagged ATR C-terminal fragment

    Depositor

    Aziz Sancar

    Article

    Unsal-Kacmaz et al Proc Natl Acad Sci U S A. 2002 May 14;99

    Insert

    ATR (ATR Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    C-terminal domain, aa 1398-2644*

    Promoter

    CMV

    Available Since

    July 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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23. pcDNA4-ATR-N

    Plasmid

    #53767

    Purpose

    mammalian expression of flag-tagged ATR N-terminal fragment

    Depositor

    Aziz Sancar

    Article

    Unsal-Kacmaz et al Proc Natl Acad Sci U S A. 2002 May 14;99

    Insert

    ATR (ATR Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    N-terminal domain, aa 1-1409

    Promoter

    CMV

    Available Since

    June 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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24. pcDNA4-ATR-M

    Plasmid

    #53768

    Purpose

    mammalian expression of flag-tagged ATR middle region

    Depositor

    Aziz Sancar

    Article

    Unsal-Kacmaz et al Proc Natl Acad Sci U S A. 2002 May 14;99

    Insert

    ATR (ATR Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    middle domain, aa 593-1700*

    Promoter

    CMV

    Available Since

    July 8, 2014

    Availability

    Academic Institutions and Nonprofits only

25. pLC133

    Plasmid

    #198639

    Purpose

    Expression plasmid for dCas9 in E. coli used in Chip-seq experiments. dCas9 is tagged with a C-terminal 3x FLAG tag. A guide RNA can be cloned using BsaI restriction sites.

    Depositor

    David Bikard

    Article

    Rostain et al Nucleic Acids Res. 2023 Mar 17:gkad170.

    Insert

    dCas9
    

    Use

    Tags

    3x-FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    pTet

    Available Since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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26. pKMD106e - intein-Cas9(S219)

    Plasmid

    #64190

    Purpose

    Expresses intein-Cas9(S219) in mammalian cells

    Depositor

    David Liu

    Article

    Davis et al Nat Chem Biol. 2015 May;11(5):316-8. doi

    Insert

    mammalian codon-optimized S. pyogenes intein-Cas9(S219) - NLS - 3x FLAG
    

    Use

    CRISPR

    Tags

    NLS - 3x FLAG (C terminal on insert)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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27. SpRY

    Plasmid

    #198544

    Purpose

    Expresses human codon-optimized SpRY and blasticidin resistance: EFS promoter-SpRY-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpRY
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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28. pJAF215

    Plasmid

    #52402

    Purpose

    Expresses Flag-tagged mouse Arf1

    Depositor

    Gregory Pazour

    Article

    Follit et al PLoS Genet. 2014 Feb 20;10(2):e1004170.

    Insert

    Arf1 (Arf1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 14, 2014

    Availability

    Academic Institutions and Nonprofits only

29. CAGhP14

    Plasmid

    #84362

    Purpose

    express FLAG-HA tagged human PRDM14

    Depositor

    Danny Reinberg

    Article

    Tu et al Nature. 2016 Jun 8;534(7607):387-90. doi

    Insert

    PRDM14 (PRDM14 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    silent mutations at coding silent 1068CtoT, 1629T…

    Promoter

    CAG

    Available Since

    Feb. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

30. EGFP in pDBEcR-pIND(SP1)/Bla

    Plasmid

    #201557

    Purpose

    Ecdysone-inducible vector with SB ITRs for expressing DBEcR (3xmyc- tagged) and FLAG- EGFP; blasticidin resistance.

    Depositor

    Randy Poon

    Article

    Yeung et al J Cell Sci. 2023 Apr 15;136(8):jcs260754

    Insert

    DBEcR and EGFP
    

    Use

    Unspecified; Sleeping beauty transposon

    Tags

    DBEcR-myc and FLAG-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a and pIMD(SP1)

    Available Since

    July 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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