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Plasmid#158615PurposeFor protein expression of BhCas12b_v4DepositorInsertNLS-BhCas12b_v4
UseTags6xHIS-TwinStrep-SUMOExpressionBacterialMutationPromoterAvailable sinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pITR-AAV-Rep2Cap9-GFP
Plasmid#199744PurposeExpresses AAV2 Rep and AAV9 Cap, with GFP replacing a region of Cap, within AAV2 ITRs.DepositorInsertAAV9 Cap w/ GFP insert
UseAAVTagsExpressionMammalianMutationReplaced amino acids 356-736 in Cap with GFPPromoterAvailable sinceOct. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
7TFP CDH1 mutant reporter
Plasmid#91703Purposelentiviral luciferase reporter containing CDH1 promoter with mutant E-boxesDepositorInsertCDH1 mutant promoter (CDH1 Human)
UseLentiviral and LuciferaseTagsluciferaseExpressionMammalianMutationmutant E-boxesPromoterCDH1 mutantAvailable sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn-DIO-mCReP
Plasmid#167503PurposeConditional expression of the eIF2alpha phophatase, CReP (Ppp1r15b)DepositorInsertPpp1r15b (Ppp1r15b Mouse)
UseAAV and Cre/LoxTagsMycExpressionMammalianMutationPromoterhSynAvailable sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
eGFP Y93H TC editing reporter
Plasmid#198882PurposeExpress eGFP fluorescence after Y93H correction and mCherry as transfection controlDepositorInsertenhanced green fluorescent protein
UseLentiviralTagsExpressionMammalianMutationY93HPromoterCMVAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPuro3.1(+)_Strep-Lrrk2-Myc
Plasmid#161583Purposeexpression of full-length mouse Lrrk2 with C-terminal Myc tagDepositorInsertLrrk2 (Lrrk2 Mouse)
UseTagsMyc tag and StrepIIExpressionMammalianMutationnonePromoterCMVAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR-CAG_3C-Twin-Strep
Plasmid#113901PurposeMammalian protein expressionDepositorTypeEmpty backboneUseLentiviralTags3C-Twin-StrepExpressionMutationPromoterCAGAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
SMN2E7-Fluc-Nluc-reporter
Plasmid#221005PurposeTransiently express SMN2 dual luciferase reporter. Inclusion of E7 produces in-frame stop codon and Firefly luciferase only, while skipping produces both Firefly and Nanoluc. CMV promoter.DepositorInsertFluc-SMN2E7-STOP-Nluc
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-StrepHA-SUMO2(Q87R)
Plasmid#66868PurposeMammalian expression of SUMO2(Q87R) with a strep and an HA tagDepositorInsertSUMO2 (SUMO2 Human)
UseTagsHA and StrepExpressionMammalianMutationQ87RPromoterCMVAvailable sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-K9 histone methylation reporter
Plasmid#22866DepositorInsertK9 HMR
UseTagsCFP and YFPExpressionMammalianMutationPromoterAvailable sinceJan. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
miRFP718nano-based inflammation reporter
Plasmid#195753PurposeReporter of inflamationDepositorInsertNF-kB-RE-miRFP718nano
UseTagsmiRFP718nanoExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFastbac-Dual-StrepII-BARD1
Plasmid#137166PurposeExpresses human StrepII-tagged BARD1 for purification from baculovirus/insect cell systemDepositorInsertBARD1 (BARD1 Human)
UseTagsStrepIIExpressionInsectMutationfully synthetic, codon optimized for insect expre…Promoterp10Available sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-SpdCas9-SBiT_Strep-Tag-II
Plasmid#198750PurposeExpresses S. Pyogenes dCas9 fused to SmallBiT fragment of split NanoLuc luciferaseDepositorInsertSpdCas9 - SmallBiT
UseTagsStrep-tag IIExpressionBacterialMutationD10A, H840A (nuclease inactivating mutations in &…PromoterT7Available sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-SpdCas9-LBiT_Strep-Tag-II
Plasmid#198751PurposeExpresses S. Pyogenes dCas9 fused to LargeBiT fragment of split NanoLuc luciferaseDepositorInsertSpdCas9 - LargeBiT
UseTagsStrep-tag IIExpressionBacterialMutationD10A, H840A (nuclease inactivating mutations in &…PromoterT7Available sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCVL.Active/Repressed TLR (Sce)
Plasmid#45575PurposeCodes for the Active/Repressed TLR with the target site for I-Sce I nucleaseDepositorInsertseGFP with I-Sce I TS
+3 mCherry
UseLentiviralTagsT2A dislinker and iRFPExpressionBacterial and MammalianMutationembedded I-Sce I TS from 163-185PromoterSFFVAvailable sinceNov. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLV-REPORT(PGK)-12xHRE
Plasmid#172334Purposehypoxic responsive reporterDepositorInsertsmonomeric nuclear Tomato
d2eGFP
HygR
UseLentiviral and Synthetic BiologyTags3x SV40 NLSExpressionMammalianMutationPromoterPGKAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOTTC414 - pAAV EF1a hPREP
Plasmid#59967PurposeAn AAV packaging vector that expresses hPREP under control of the EF1a promoter.DepositorInsertProlyl oligopeptidase (PREP Human)
UseAAVTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRepair-MPP8_mAID-P2A-BFP
Plasmid#175036PurposeHomology Directed Repair construct for C-terminal tagging of mMPP8 with mAID using CRISPR/Cas9DepositorInsertmAID-P2A-BFP
UseHdr donor templateTagsExpressionMutationPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-2xFLAG-2xSTREP-CCND1
Plasmid#172648PurposeExpresses 2xFLAG-2xSTREP-tagged cyclin D1 in mammalian cellsDepositorInsertCCND1 (CCND1 Human)
UseTags2xFLAG-2xSTREPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-2xFLAG-2xSTREP-CDK6
Plasmid#172614PurposeExpresses 2xFLAG-2xSTREP-tagged CDK6 in mammalian cellsDepositorInsertCDK6 (CDK6 Human)
UseTags2xFLAG-2xSTREPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-TMPRSS2-TwinStrep_Zeo
Plasmid#192000PurposeLentiviral vector to generate TwinStrep-tagged TMPRSS2 stable expressing cell lineDepositorInsertTMPRSS2 (TMPRSS2 Human)
UseLentiviralTagsTMPRSS2ExpressionMammalianMutationPromoterAvailable sinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSIN REP5-GFP-GluR2 (Q)
Plasmid#24003DepositorInsertGluR2 (Gria2 Rat)
UseSindbis virus productionTagsGFPExpressionMutationR607QPromoterAvailable sinceApril 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)_Strep-Lrrk2-Myc
Plasmid#161582Purposeexpression of full-length mouse Lrrk2 with C-terminal Myc tagDepositorInsertLrrk2 (Lrrk2 Mouse)
UseTagsMyc tag and StrepIIExpressionMammalianMutationnonePromoterCMVAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHS0663 pET45b(+) KraIscB-1 TwinStrep
Plasmid#176536PurposeBacterial expression of KraIscB-1-TwinStrepDepositorInsertKraIscB-1
UseTagsTwinStrepExpressionBacterialMutationPromoterAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJC5-4-insulator-BREreporter-insulator
Plasmid#171499Purposeinjections to make Bmp reporter mouse transgenic lineDepositorInsertInsulator BRE-MLP:H2B-Turqoise
UseTagsExpressionMammalianMutationPromoterBRE-MLPAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-StrepII-TagRFP-CohI
Plasmid#58710PurposeDetection construct to label dockerin type I containing fusion proteinsDepositorInsertTagRFP-CohI
UseTagsStrepIIExpressionBacterialMutationPromoterT7Available sinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
Ki67∆repeats-mNeonGreen-IRESpuro2
Plasmid#183742PurposeExpresses Ki67∆repeats-mNeonGreenDepositorInsertMKI67(lacking repeats) (MKI67 Human)
UseTagsmNeonGreenExpressionBacterial and MammalianMutationMKI67(lacking repeats)PromoterCMVAvailable sinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMIR-Report-Luc-KLF4-FL
Plasmid#34597DepositorInsertKLF4 (KLF4 Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
SOX2 TALE Repressor (KRAB 1-75)
Plasmid#42945DepositorInsertSOX2 TALE Repressor (KRAB 1-75)
UseLentiviralTags2A-GFPExpressionMammalianMutationPromoterEF-1aAvailable sinceFeb. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
sgRNA1_Tet-inducible Luciferase reporter
Plasmid#64161PurposePhotoactivatable transcription system. Mammalian expression of sgRNA1 to target Tet-inducible-luciferase reporter.DepositorInsertsgRNA1 for Tet-inducible Luciferase reporter
UseCRISPRTagsExpressionMammalianMutationPromoterhuman U6Available sinceJune 23, 2015AvailabilityAcademic Institutions and Nonprofits only